IMAC 法による塩分を含む液からのアンセリン䊶 *ルノシンの回収

⎇ⓥ⠪䉲䊷䉵⚫੺
ችፒᄢቇᎿቇㇱ‛⾰ⅣႺൻቇ⑼
ᄢፉ䇭㆐਽
⎇ⓥಽ㊁䇭䇭ಽ㔌Ꮏቇ䋬 ಽሶ⹺⼂ൻቇ
⎇ⓥ᭎ⷐ䇭䇭ᣂⷙ෻ᔕಽ㔌᧚ᢱ 䋨᛽಴೷ 䊶 ๆ⌕೷䋩 䈱㐿⊒
㪠㪤㪘㪚 ᴺ䈮䉋䉎Ⴎಽ䉕฽䉃ᶧ䈎䉌䈱䉝䊮䉶䊥䊮 䊶 䉦䊦䊉䉲䊮䈱࿁෼
᫢Լь߻ࡑ෩ẦỤỴὅἍἼὅ Ὁ ỽἽἠἉὅử‫ׅ‬ӓẴỦỆỊᾎ
ᶏᵗᕈࡃࠗࠝࡑࠬߩਛߢ‫ߩߤߥࠝ࠷ࠞޔࡠࠣࡑޔ‬࿁ㆆ㝼߇᦭ߔࠆ
ࠕࡦ࠮࡝ࡦ‫ߪࡦࠪࡁ࡞ࠞ߮ࠃ߅ޔ‬ఝࠇߚஜᐽᯏ⢻ࠍ᦭ߒ߹ߔ‫ޕ‬
H3N
H
N
COOCH2
N
NH
Metal
ion
ᧄ⎇ⓥߢߪႮಽࠍ฽߻ᾚ᳝߆ࠄߢ߽
ࠕࡦ࠮࡝ࡦ࡮ࠞ࡞ࡁࠪࡦࠍๆ⌕࿁෼
ߢ߈ࠆ̌㊄ዻᜂᜬ᮸⢽̍ߦࠃߞߡ
Cation Exchange
Resin
ࡍࡊ࠴࠼߇Ⴎ㘃ࠍ฽߻ᶧ߆ࠄ߽
ㆬᛯ⊛ߦ࿁෼ߢ߈ࠆߎߣࠍ
Fig. 1 Schematic illustration of Car adsorption
᣿ࠄ߆ߦߒ߹ߒߚ‫ޕ‬
by immobilized metal affinity adsorption.
(1.36 mmol/g of Cu(II)
was immobilized on
Cu-IDA resin.)
CH2N
Car(Cu-IDA,NaCl฽᦭)
100
㘩Ⴎ㕖ሽ࿷ਅ
80
40
CH2COOH
Fig. 3 Cation exchange resins and the Cu(II)
immobilized resins.
1. Ⴎ㘃฽᦭ṁᶧ߆ࠄߩࡅࠬ࠴ࠫࡦ฽᦭ࡍࡊ࠴࠼ߩ࿁෼ᣇᴺ̍․㗿 㧘(2007)
2. T. Oshima, K. Kanemaru, K. Ohe, Y. Baba, Adsorption Behavior of Carnosine and
Anserine on Copper (II) immobilized cation exchange resins, J. Ion Exch., in press (2007)
Lys
Arg
Ans
His
Car
Tyr
Phe
Ile
Leu
Met
Val
Cys
Ala
Gly
Glu
Pro
Ser
0
Thr
20
CH2COOH
Fig. 2 Molecular structure
of IDA resin.
㘩Ⴎሽ࿷ਅ
60
Asp
CH2 CH
ㅢᏱߩࠗࠝࡦ੤឵᮸⢽ߪ
㕒㔚⊛⋧੕૞↪ࠍᒁജߣ
Ans(Cu-IDA,NaCl฽᦭) ߔࠆߚ߼‫ޔ‬Ⴎ㘃߇ṁߌߚ
60
ṁᶧߢߪࡍࡊ࠴࠼ߩๆ⌕
40
ജ߇⪺ߒߊૐਅߒ߹ߔ‫ޕ‬
৻ᣇ‫ޔ‬㊄ዻᜂᜬ᮸⢽ߦࠃ
20
ࠆๆ⌕ߪ㈩૏⚿วߦࠃࠆ
0
ߚ߼‫ޔ‬Ⴎಽ߇౒ሽߔࠆਛ
2
4
6
8
10
12
߆ࠄߢ߽ࠕࡦ࠮࡝ࡦ࡮ࠞ
pHeq
Fig. 4 Adsorption profiles of Car and Ans ࡞ࡁࠪࡦࠍๆ⌕ߢ߈߹ߔ
on IDA and Cu-IDA ([NaCl] = 100 mM) 㧔(KI‫ޕ‬
Ans(IDA,NaCl฽᦭)
80
C
H2
O
Car(IDA,NaCl฽᦭)
Ads/%
H2
C
+
100
% Adsorption [%]
ᑄ᫈‛ࠍේᢱߣߒߡࠕࡦ࠮࡝ࡦ╬ࠍ
♖⵾ߒ೑↪ߔࠆߚ߼ߦߪ‫ޔ‬
ઁߩᚑಽ߆ࠄ⋡⊛ߩࠕࡦ࠮࡝ࡦ࡮
ࠞ࡞ࡁࠪࡦߛߌࠍ
ㆬᛯ⊛ߦขࠅ಴ߔᛛⴚ߇ᔅⷐߢߔ‫ޕ‬
‫ط‬ửԃớ෩ẦỤỴὅἍἼὅ Ὁ ỽἽἠἉὅử‫ׅ‬ӓẴỦ
Fig. 5 Adsorption percent of amino acids and dipeptides from a mix
on Cu-IDA (pH 8.0).
ࠕࡒࡁ㉄㧝㧣⒳‫ ߮ࠃ߅ޔ‬#PU࡮%CT ߇ฦ O/ ฽߹ࠇߚᷙว
ᶧ߆ࠄߩ %W+&# ᮸⢽ߦࠃࠆๆ⌕ታ㛎ߩ⚿ᨐ߇ (KI ߢߔ‫ޕ‬
㧝㧥⒳ߩࠕࡒࡁ㉄࡮ࡍࡊ࠴࠼ߢ *KU#PU%CT ߇ㆬᛯ⊛ߦ
ๆ⌕ߐࠇࠆߎߣ߇␜ߐࠇ߹ߒߚ‫ޕ‬0C%N ߇ O/ ฽߹ࠇࠆᶧ
߆ࠄ߽ߎࠇࠄߩࠕࡒࡁ㉄࡮ࡍࡊ࠴࠼ߪๆ⌕ߐࠇ߹ߔ‫ޕ‬
䊋䉪䊁䊥䉝䉶䊦䊨䊷䉴⺃ዉ૕
䈱⺞⵾䈫䉺䊮䊌䉪⾰䈱ๆ⌕
ἢἁἘἼỴἍἽἿὊἋỉࣇኬጞዜನᡯỂ
᭗ܾ᣽ỆἑὅἣἁឋửԈბẴỦ
Fig. 7 SEM image of phosphorylated plant
cellulose (PPC) at 25,000 magnification
40
O
OH
P
CH2O OH
CH2OH
O
OH
O
+
O
OH
n
H3PO4
(NH2)2CO
DMF
OH
+
O
OH
H2O
40
PPC
PC
30
q [μmol g-1]
ᧄ⎇ⓥߢߪ࡝ࡦ㉄ൻࡃࠢ࠹࡝ࠕ࠮࡞ࡠ࡯ࠬࠍ
⺞⵾ߒ‫⾰ࠢࡄࡦ࠲ޔ‬ๆ⌕೷ߣߒߡߩๆ⌕․ᕈࠍ
⹏ଔߒߚ‫ޕ‬
࡝ࡦ㉄ൻࡃࠢ࠹࡝ࠕ࠮࡞ࡠ࡯ࠬߪ࡝ࡦ㉄ൻᐲߩ
඘ᢜߔࠆᬀ‛↱᧪ߩ࡝ࡦ㉄࠮࡞ࡠ࡯ࠬߣᲧセߒߡ
⚂㧞୚ߩๆ⌕ኈ㊂ࠍ␜ߒߚ‫ޕ‬Ყセ⊛ᄢ߈޿
࠲ࡦࡄࠢ⾰ߪ❫⛽⴫㕙ߢๆ⌕ߐࠇࠆߚ߼‫ޔ‬ᓸ⚦
❫⛽߆ࠄߥࠆๆ⌕೷߇㜞ኈ㊂ࠍ␜ߔߣ⠨߃ࠄࠇࠆ‫ޕ‬
Fig. 8 SEM image of phosphorylated bacterial
cellulose (PBC) at 25,000 magnification
PBC
BC
30
q [μmol g-1]
㈶㉄⩶ߦઍ⴫ߐࠇࠆ⩶૕߇↥಴ߔࠆ̍ࡃࠢ࠹࡝
ࠕ࠮࡞ࡠ࡯ࠬ̍ߪ‫ޔ‬ᬀ‛↱᧪ߩ࠮࡞ࡠ࡯ࠬߣ
Ყセߒߡ 㨪 㧘❫⛽᏷ ǴO ߩᓸ⚦
❫⛽߆ࠄߥࠆ‫ޕ‬
20
10
20
10
0
0
0
50
100
150
Ceq [μmol dm-3]
200
0
50
100
150
Ceq [μmol dm-3]
200
Fig. 9 Adsorption isotherms of lysozyme onto Fig. 10 Adsorption isotherms of lysozyme onto
PPC and PC at 30ºC
PBC and BC at 30ºC
a)
b)
n
Fig. 6 The representative synthetic route of
phosphorylated cellulose
3. T. Oshima, S. Taguchi, H. Fujiwara, K. Ohe, Y. Baba,
Adsorption Behaviors of Bioactive Amines and Proteins on
Phosphorylated Bacterial Cellulose, J. Ion Exch., in press (2007)
Fig. 11 Conceptual illustration of protein adsorption on PPC (a) and PBC (b).