⎇ⓥ⠪䉲䊷䉵⚫ ችፒᄢቇᎿቇㇱ‛⾰ⅣႺൻቇ⑼ ᄢፉ䇭㆐ ⎇ⓥಽ㊁䇭䇭ಽ㔌Ꮏቇ䋬 ಽሶ⼂ൻቇ ⎇ⓥⷐ䇭䇭ᣂⷙᔕಽ㔌᧚ᢱ 䋨 䊶 ๆ⌕䋩 䈱㐿⊒ 㪠㪤㪘㪚 ᴺ䈮䉋䉎Ⴎಽ䉕䉃ᶧ䈎䉌䈱䉝䊮䉶䊥䊮 䊶 䉦䊦䊉䉲䊮䈱࿁ Լьࡑ෩ẦỤỴὅἍἼὅ Ὁ ỽἽἠἉὅửׅӓẴỦỆỊᾎ ᶏᵗᕈࡃࠗࠝࡑࠬߩਛߢߩߤߥࠝ࠷ࠞޔࡠࠣࡑޔ࿁ㆆ㝼߇ߔࠆ ࠕࡦࡦߪࡦࠪࡁ࡞ࠞ߮ࠃ߅ޔఝࠇߚஜᐽᯏ⢻ࠍߒ߹ߔޕ H3N H N COOCH2 N NH Metal ion ᧄ⎇ⓥߢߪႮಽࠍᾚ᳝߆ࠄߢ߽ ࠕࡦࡦࠞ࡞ࡁࠪࡦࠍๆ⌕࿁ ߢ߈ࠆ̌㊄ዻᜂᜬ᮸⢽̍ߦࠃߞߡ Cation Exchange Resin ࡍࡊ࠴࠼߇Ⴎ㘃ࠍᶧ߆ࠄ߽ ㆬᛯ⊛ߦ࿁ߢ߈ࠆߎߣࠍ Fig. 1 Schematic illustration of Car adsorption ࠄ߆ߦߒ߹ߒߚޕ by immobilized metal affinity adsorption. (1.36 mmol/g of Cu(II) was immobilized on Cu-IDA resin.) CH2N Car(Cu-IDA,NaCl) 100 㘩Ⴎ㕖ሽਅ 80 40 CH2COOH Fig. 3 Cation exchange resins and the Cu(II) immobilized resins. 1. Ⴎ㘃ṁᶧ߆ࠄߩࡅࠬ࠴ࠫࡦࡍࡊ࠴࠼ߩ࿁ᣇᴺ̍․㗿 㧘(2007) 2. T. Oshima, K. Kanemaru, K. Ohe, Y. Baba, Adsorption Behavior of Carnosine and Anserine on Copper (II) immobilized cation exchange resins, J. Ion Exch., in press (2007) Lys Arg Ans His Car Tyr Phe Ile Leu Met Val Cys Ala Gly Glu Pro Ser 0 Thr 20 CH2COOH Fig. 2 Molecular structure of IDA resin. 㘩Ⴎሽਅ 60 Asp CH2 CH ㅢᏱߩࠗࠝࡦ឵᮸⢽ߪ 㕒㔚⊛⋧↪ࠍᒁജߣ Ans(Cu-IDA,NaCl) ߔࠆߚޔႮ㘃߇ṁߌߚ 60 ṁᶧߢߪࡍࡊ࠴࠼ߩๆ⌕ 40 ജ߇⪺ߒߊૐਅߒ߹ߔޕ ৻ᣇޔ㊄ዻᜂᜬ᮸⢽ߦࠃ 20 ࠆๆ⌕ߪ㈩⚿วߦࠃࠆ 0 ߚޔႮಽ߇ሽߔࠆਛ 2 4 6 8 10 12 ߆ࠄߢ߽ࠕࡦࡦࠞ pHeq Fig. 4 Adsorption profiles of Car and Ans ࡞ࡁࠪࡦࠍๆ⌕ߢ߈߹ߔ on IDA and Cu-IDA ([NaCl] = 100 mM) 㧔(KIޕ Ans(IDA,NaCl) 80 C H2 O Car(IDA,NaCl) Ads/% H2 C + 100 % Adsorption [%] ᑄ᫈‛ࠍේᢱߣߒߡࠕࡦࡦ╬ࠍ ♖ߒ↪ߔࠆߚߦߪޔ ઁߩᚑಽ߆ࠄ⋡⊛ߩࠕࡦࡦ ࠞ࡞ࡁࠪࡦߛߌࠍ ㆬᛯ⊛ߦขࠅߔᛛⴚ߇ᔅⷐߢߔޕ طửԃớ෩ẦỤỴὅἍἼὅ Ὁ ỽἽἠἉὅửׅӓẴỦ Fig. 5 Adsorption percent of amino acids and dipeptides from a mix on Cu-IDA (pH 8.0). ࠕࡒࡁ㉄㧝㧣⒳ ߮ࠃ߅ޔ#PU%CT ߇ฦ O/ ߹ࠇߚᷙว ᶧ߆ࠄߩ %W+&# ᮸⢽ߦࠃࠆๆ⌕ታ㛎ߩ⚿ᨐ߇ (KI ߢߔޕ 㧝㧥⒳ߩࠕࡒࡁ㉄ࡍࡊ࠴࠼ߢ *KU#PU%CT ߇ㆬᛯ⊛ߦ ๆ⌕ߐࠇࠆߎߣ߇␜ߐࠇ߹ߒߚޕ0C%N ߇ O/ ߹ࠇࠆᶧ ߆ࠄ߽ߎࠇࠄߩࠕࡒࡁ㉄ࡍࡊ࠴࠼ߪๆ⌕ߐࠇ߹ߔޕ 䊋䉪䊁䊥䉝䉶䊦䊨䊷䉴⺃ዉ 䈱⺞䈫䉺䊮䊌䉪⾰䈱ๆ⌕ ἢἁἘἼỴἍἽἿὊἋỉࣇኬጞዜನᡯỂ ᭗ܾỆἑὅἣἁឋửԈბẴỦ Fig. 7 SEM image of phosphorylated plant cellulose (PPC) at 25,000 magnification 40 O OH P CH2O OH CH2OH O OH O + O OH n H3PO4 (NH2)2CO DMF OH + O OH H2O 40 PPC PC 30 q [μmol g-1] ᧄ⎇ⓥߢߪࡦ㉄ൻࡃࠢ࠹ࠕ࡞ࡠࠬࠍ ⺞ߒ⾰ࠢࡄࡦ࠲ޔๆ⌕ߣߒߡߩๆ⌕․ᕈࠍ ⹏ଔߒߚޕ ࡦ㉄ൻࡃࠢ࠹ࠕ࡞ࡠࠬߪࡦ㉄ൻᐲߩ ᢜߔࠆᬀ‛↱᧪ߩࡦ㉄࡞ࡠࠬߣᲧセߒߡ ⚂㧞ߩๆ⌕ኈ㊂ࠍ␜ߒߚޕᲧセ⊛ᄢ߈ ࠲ࡦࡄࠢ⾰ߪ❫⛽㕙ߢๆ⌕ߐࠇࠆߚޔᓸ⚦ ❫⛽߆ࠄߥࠆๆ⌕߇㜞ኈ㊂ࠍ␜ߔߣ⠨߃ࠄࠇࠆޕ Fig. 8 SEM image of phosphorylated bacterial cellulose (PBC) at 25,000 magnification PBC BC 30 q [μmol g-1] ㈶㉄⩶ߦઍߐࠇࠆ⩶߇↥ߔࠆ̍ࡃࠢ࠹ ࠕ࡞ࡠࠬ̍ߪޔᬀ‛↱᧪ߩ࡞ࡠࠬߣ Ყセߒߡ 㨪 㧘❫⛽ ǴO ߩᓸ⚦ ❫⛽߆ࠄߥࠆޕ 20 10 20 10 0 0 0 50 100 150 Ceq [μmol dm-3] 200 0 50 100 150 Ceq [μmol dm-3] 200 Fig. 9 Adsorption isotherms of lysozyme onto Fig. 10 Adsorption isotherms of lysozyme onto PPC and PC at 30ºC PBC and BC at 30ºC a) b) n Fig. 6 The representative synthetic route of phosphorylated cellulose 3. T. Oshima, S. Taguchi, H. Fujiwara, K. Ohe, Y. Baba, Adsorption Behaviors of Bioactive Amines and Proteins on Phosphorylated Bacterial Cellulose, J. Ion Exch., in press (2007) Fig. 11 Conceptual illustration of protein adsorption on PPC (a) and PBC (b).
© Copyright 2024 ExpyDoc