Cytek Development DxP Flow Cytometry Systems Designed specifically for BD FACScanTM, FACSortTM and FACSCaliburTM flow cytometers, Cytek’s Digital Extra Parameter (DxP) upgrades are scalable up to five lasers and fourteen fluorescent detection channels. DxP customizable configurations and add-on options support a wide range of applications. By leveraging the use of existing proven fluidics, DxP systems are a cost effective alternative to buying a new cytometer. For more information visit Cytek at www.cytekflow.com. Cytek Development DxP Flow Cytometry Systems Optics Fluidics Performance Excitation Optics Sample Flow Rates Fluorescence Sensitivity Optical Platform Accommodates up to four lasers. Consists of a fixed optical assembly with three spatially separated laser beams. FACSCalibur Front panel keypad provides three modes: RUN, STANDBY, and PRIME Three preset flow rates: LO (12 μL/min) MED (35 μL/min) HI (60 μL/min) Molecules of Equivalent Fluorescence (MEFL) using Q&b Method. FACSort Front panel knob provides five modes: RUN, STANDBY, DRAIN, FILL, BACKFLUSH Three preset flow rates: LO (12 μL/min) MED (35 μL/min) HI (60 μL/min) FACScan Front panel knob provides five modes: RUN, STANDBY, DRAIN, FILL, BACKFLUSH Two preset flow rates: LO (12 μL/min) HI (60 μL/min) * Assumes no compensation applied. Standard Fluidic Reservoirs Both a four liter sheath container and a four liter waste container are provided. Forward and Side Scatter Sensitivity This feature enables separation of fixed platelets from noise. Recommended Fluidics Option Cytek Fluid Manager supply system is an automated fluidics system including twenty liter containers and a precise air system for ultra-stable sheath velocity. Resolution 18 bit 5 log decades Lasers 355nm, 407nm, 445nm 488nm, 515nm, 532nm 553nm, 561nm, 592nm 637nm, 660nm, 671nm Other lasers available upon request. Beam Geometry Uses a prismatic expander and achromatic spherical lens. Optical Efficiency Power loss at flow cell is less than 20% of specified laser power. Flow Cell Design The rectangular fused silica cuvette has an anti-reflective coating on external surfaces. The internal cross-section is 430 x 180 μm. Emission Optics Optical Coupling Fused silica cuvette is coupled to emission lens by refractive index matching optical gel for 2.6 str solid angle collection. Background Rejection Includes obscuration blade and slit for minimizing unwanted laser radiation at the FSC detector. Forward Scatter Detector and Filter Features a high-performance, solid state silicon detector with 488 nm bandpass filter. Side Scatter Detector Utilizes a photomultiplier with a Brewsterangle beam splitter and 488 nm bandpass filter. Fluorescence Detectors and Filters DxP10 standard configuration: BluFL1: 530/30 (FITC) BluFL2: 585/42 (PE) BluFL3: 620/20 (PE-TxRD) BluFL4: 695-40 (PE-CY5.5) BluFL5: 780/60 (PE-CY7) RedFL1: 660/20 (APC) RedFL2: 725/20 (Alexa 700) RedFL3: 780/60 (APC-CY7) VioFL1: 450/50 (PAC Blue) VioFL2: 545/30 (PAC Orange) Custom configurations available upon request. Fluor Min. Q Max. b FITC 0.0071250 PE 0.071400 PE-CY5 0.0056500 PE-CY7 0.0007250 APC 0.008250 APC-CY7 0.00151500 PAC Blue 0.0025 15000 PAC Orange 0.0005 15000 Fluorescence Linearity Delivers doublet/singlet ratio of 1.95–2.05 for CEN stained with PI and excited with the 488 nm blue laser. Data Acquisition Rate 10,000 events/sec with beads Cytek Development DxP Flow Cytometry Systems Data Management Workstation Options FlowJo Collectors’ Edition 7.5.110 or Later Operating System Windows® 7 Professional 32-bit With our intuitive acquisition interface, new users will be acquiring data with minimal training. Processor Intel Core i5 quad core processor 96 Well Auto-Sampling Cytek’s Automatic Micro-Sampler (AMS) can be mounted directly under the sample injection tube reducing dead volume and increasing throughput. We provide a real time spillover matrix for viewing live compensated data. Acquisition templates include hardware, gate, spillover, layout and statistical settings. Increase efficiency by saving FlowJo workspaces for use during multiple acquisition sessions. Cytek AMS Software Version 1.0.2 Fast and Easy Setup Experiment plate mapping can be setup on a stand-alone computer and saved as a template for future use. Block Inspector You can change the FlowJo Collectors’ Edition acquisition settings on a per block basis. Well ID Stamping Incorporating the Well ID in the file name confirms data file to Well ID relationship. Memory and Storage 4 GB RAM, Dual 500GB SATA hard drives DVD+/-RW Monitors Standard 24” LCD Optional 27” High Resolution LCD Optional Dual Monitors Acquisition Times Auto-mode: Complete a full 96 well plate in 39 minutes (15 second acquisition) with less than 1% carryover. High throughput mode: Complete a full 96 well plate in 15 minutes (5 second acquisition) with less than 3% carryover. Kinetics Module Measure calcium flux and other kinetics experiments using Cytek’s Time Zero System. Time Zero supports in test tube agonist injection and mixing, sample temperature control, sample boosting, and time marking. Fluid Manager Get an additional 14.8 hours of continuous acquisition time by using larger supply and waste carboys for the cytometer’s sheath fluid flow. The precision air regulation system ensures continuous sheath flow and precise laser delays. Cytek Development DxP Flow Cytometry Systems Installation Requirements Dimensions (W x D x H) FACSCalibur Sensor module 91.4 x 61.5 x 67.3 cm (36 x 24.2 x 26.5 in) Height with cover option: 124.5 cm (49 in) Computer 48 x 41 x 58 cm (19 x 16 x 23 in) Weight Sensor module: 109.1 kg (~240 lb) Computer: varies with configuration Recommended Workspace 180 L x 91 D x 132 cm H (71 x 36 x 52 in) FACSort Sensor module 91 L x 60 D x 51 cm H (36 L x 23.5 D x 20 in H) Computer 48 x 41 x 58 cm (19 x 16 x 23 in) Weight Sensor module: 99 kg (~199 lb) Computer: varies with configuration Recommended Workspace 180 L x 91 D x 132 cm H (71 x 36 x 52 in) FACScanTM, FACSortTM , and FACSCaliburTM are trademarks of Becton Dickinson Windows is a registered trademark of Microsoft Corporation in the United States and other countries. Regulatory Disclaimer FACScan Sensor Module 76 L x 60 D x 51 cm H (30 L x 23.5 D x 20 in H) Intended for research purposes only. Not for use in diagnostic or therapeutic procedures. Computer 48 x 41 x 58 cm (19 x 16 x 23 in) Weight Sensor module: 61 kg (~135 lb) Computer: varies with configuration Recommended Workspace 160 L x 84 D x 86 cm H (63 x 33 x 34 in) Power US: 120 VAC ±10%; 50/60 Hz ±2 Hz Current: 20 amp maximum Outside US: External transformer needed for 100 VAC ±10%; 50/60 Hz ±2 Hz or 220/240 VAC ±10%; 50/60 Hz ±2 Hz. Heat Dissipation 6,500 BTU/h with argon laser; 2000 BTU/h with all solid state lasers Temperature 16–29°C (60–85°F) Humidity 10% to 90% relative non-condensing Air Filtering No excessive dust and smoke Lighting Optics and detectors shielded from room Cytek Development 4059 Clipper Ct, Fremont, CA 94538 (877) 92-CYTEK [email protected]