Adhezija ćelija mišjih fibroblasta na površinu novog

Cell adhesion of mouse fibroblasts
to the surface of a novel hydroxyapatite/fullerenol
nanocomposite
Aleksandar N. Djordjević, Nenad L. Ignjatović*, Gordana B. Bogdanović**, Mariana N. Seke***, Dragan P.
Uskoković*, Danica S. Jović, Zlatko Lj. Rakočević***
Faculty of Science, Department of Chemistry, Biochemistry and Environmental Protection, University of Novi Sad, Serbia,
*Centre for Fine Particles Processing and Nanotechnologies, Institute of Technical Science of the Serbian Academy of Science and
Arts, Belgrade, Serbia,
**Oncology Institute of Vojvodina, Sremska Kamenica, Serbia,
***Vinča Institute of Nuclear Science, University of Belgrade, Vinča, Serbia
Goal
To examine the difference in the ability
of mouse fibroblasts (L929) to adhere to
discs of hydroxyapatite (HAp) and
hydroxyapatite/fullerenol
nanocomposite (HAp/FNP)
Motivation
HAp is the main inorganic component of bone material
FNP is hydrophilic polyanion; has anti-inflammatory, antioxidant and proliferative
properties
Cell adhesion expands better on negatively charged, hydrophilic surfaces
HAp/FNP particles will express good surface properties for enhanced adhesion of
fibroblasts
Application
Biocompatible material for biomedical applications e.g. osteo implants/coatings
Experimental
Adhesion of mouse fibroblasts to the surfaces of HAp and HAp/FNP was tested in vitro.
Fibroblasts adhesion was measured after 72 hour-incubation with materials in particular cell medium
and incubation conditions.
Cells were analysed by low voltage scanning electron microsopy-SEM, JEOL-JSM 6460LV.
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Figure 1. Cell adhesion and growth morphology of mouse fibroblasts (L929)
on the HAp surface
Results
Growing on the HAp/FNP nanocomposite surface, mouse
fibroblast cells (L929) responded with better adherence,
spreading and formation of a continuous network with the
underlying surface (Figure 2) than in case of HAp surface
(Figure 1).
Permeation of the cytoplasmic protrusions not only on the
surface of HAp/FNP, but also between fibroblasts, indicates
proliferative potential
of these cells (Figure 2c).
Figure 2 clearly shows the compactness of the surface,
pointing out the stability of materials under conditions of cell
growth and microscopic preparation.
Acknowledgments
This work was done as a part of scientific projects of Ministry of Education and Science, Serbia, III
45005 and 45004.
Figure 2. Cell adhesion and growth morphology of mouse fibroblasts (L929)
on the hydroxyapatite/fullerenol nanocomposite surface
Conclusions
Surfaces of HAp and HAp/FAP nanocomposites have various effects
on cell adhesion.
Number, voluminosity and mutual proximity of cells indicate better
adhesion of mouse fibroblasts on HAp/FNP surface in comparison to
HAp.
Negatively charged surface promotes formation of highly periodic
filaments.
HAp/FNP nanocomposite contains FNP, which due to its antiinflammatory, antioxidant and proliferative properties can improve cell
adhesion.
The novel HAp/FNP nanocomposite has a great potential to be used
as an initial powder source for the production of the array of
nanocomposites and coatings.
FIRST INTERNATIONAL CONFERENCE OF YOUNG CHEMISTS OF SERBIA
October 19 – 20, 2012, Belgrade, Serbia
E-mail: [email protected]
Phone: 00381214852784, 00381214852759

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