S-Monovette® HCY-C

S-Monovette® HCY-C
Evaluation Report
Prepared by:
SARSTEDT AG & Co.
Hyperhomocysteinaemia has been established as an important, independent risk factor for
atherosclerotic and neurodegenerative diseases.
Fig. 1:
tHCY recovery in
EDTA blood when stored
at room temperature
n = 11 donors,
with simple standard
deviation
% recovery
For diagnostic purposes, the total homocysteine concentration (tHCY) in the blood plasma is
determined. During this procedure, compliance with preanalytical requirements is imperative
because, even after blood collection, homocysteine is continually exported from the
erothrocytes into the plasma. Specialist medical literature1 indicates an increase of approximately
10 % per hour. This fact has been substantiated in in-house tests (FPIA, Abbott IMx):
The new S-Monovette® HCY-C (Art. No. 04.1911.001) with Acidic Citrate Solution (pH 4.3)
now enables stabilization of homocysteine concentration in the blood sample. Blood collection
is carried out to the well-tried and reliable S-Monovette® principle. For preparatory reasons,
the raw measuring value established after analysis must be multiplied by the dilution factor
1.11 in order to obtain the diagnostic homocysteine value.
Fig. 2:
tHCY recovery in
Acidic Citrate Blood
when stored at
room temperature
n = 13 donors,
with simple standard
deviation
% recovery
According to specialized medical literature2, this citrate solution stabilizes the homocysteine
value for 6 hours at room temperature. This fact, too, has been verified in internal tests
(FPIA, Abbott IMx):
Fig. 3:
tHCY recovery in Acidic
Citrate Blood when
stored at 4 °C
n = 10 donors,
with simple standard
deviation
% recovery
It is also known that sample storage at 4 °C stabilizes the homocysteine values in Acidic
Citrate Blood for 48 hours:
Fig. 4:
tHCY recovery in Acidic
Citrate Blood when
first stored at room
temperature for 6 hours
and later on at 4°C
n = 13 donors,
with simple standard
deviation
% recovery
This can also be confirmed if the samples are first stored at room temperature and later on
kept in a refrigerated environment at 4 °C:
List of References:
1
A. Andersson, A. Isaksson and B. Hultberg:
Homocysteine Export from Erythrocytes and its Implication for Plasma Sampling
Clin Chem 1992, 38, 1311-1315.
P. Ueland, H. Refsum, S. P. Stabler, M. R. Malinow, A. Andersson and R. H. Allen:
Total Homocysteine in Plasma or Serum: Methods and Clinical Applications
Clin Chem 1993, 39, 1764-1779.
2
H. P. J. Willems, G. M. J. Bos, W. B. J. Gerrit, M. den Heijer, S. Vloet and H. J. Blom:
Acidic Citrate Stabilizes Blood Samples for Assay of Total Homocysteine
Clin Chem 1998, 44, 342-345.
M. Nauck, E. Bisse and H. Wieland:
Pre-analytical Conditions Affecting the Determination of the Plasma Homocysteine Concentration
Clin Chem Lab Med 2001, 39, 675-680.
H. P. J. Willems, M. den Heijer, J. Lindemans, H. W. A. Berenschot, W. B. J. Gerrits, G. M. J. Bos, H. J. Blom:
Measurement of Total Homocysteine Concentrations in Acidic Citrate- and EDTA-Containing Tubes by Different Methods
Clin Chem 2004, 50, 1881-1883.
422a-200-0106-0909
This document may contain information on products that may not be available in particular countries
Technical modiﬁcations reserved
Measurement of homocysteine values (in μmol/L) from S-Monovette® HCY-C (0 and 6 h at
room temperature) vs. S-Monovette® EDTA (0 h, x-axis) in an instrument comparison of
IMx (Abbott), AxSYM (Abbott), Synchron (Beckmann Coulter) and Immulite 2000 (DPC):
methodical ± 3s precision range as specified by the instrument manufacturers (Inter-Assay)
HCY-C 0 h with straight regression line
HCY-C 6 h with straight regression line
SARSTEDT AG & Co.
P.O. Box 12 20
D-51582 Nümbrecht
Phone +49 2293 3050
Fax
+49 2293 305- 122
[email protected]
www.sarstedt.com

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