Cell Culture Products Catalogue 2014 www.capricorn-scientific.com 2 Capricorn Scientific The New Standard in Cell Culture Capricorn Scientific GmbH is a privately held, innovative company which was established in 2013 and will set a new standard in the field of cell culture. We manufacture, develop and worldwide distribute products for the cultivation, storage and separation of cells and for specific applications in research, development, diagnostic and biopharmaceutical production. We are committed to providing the high quality, reliability, availability and service you have come to expect from a globally active cell culture company. The Quality Expert Knowledge The mission of Capricorn Scientific is to provide If you are looking for a competent partner in quality cell culture media components, products, the field of cell culture, we are the right choice. and services using the highest safety and com- Our qualified staff has substantial experience pliance standards to meet the needs of our and expertise in various areas of the life customers in the global bioprocessing and clini- sciences. With our team, we cover all relevant cal diagnostic industries. business areas to support our customers’ Capricorn Scientific gathers raw materials needs. Technical Service, Product Management from a variety of sources. We have access to and Business Development are positions to EU-approved, Australian and USA based bovine service our customers. herds. Serum for Cell Culture is a question of confidence! We work closely together with raw material providers to ensure that we only accept safe and well documented products. For our serum products, we use the latest equipment and techniques to pool, treat and filter under contract bovine sera. Our expert staff oversees each stage of the process and quality control test the final product. As we have carefully cultivated a consistent supply of raw material, you can expect a consistent supply of the product you need. Capricorn Scientific delivers the highest Benefit from • High quality products • Expert knowledge • Experienced Technical Service • P erson-to-person contact in customer service department • C ompetence in logistics and import/export regulations Environmental friendly We are striving to reduce the use of paper whenever possible in order to save on natural quality cell culture media products to its cus- resources and to reduce the wastewater load. tomers by providing three key benefits: safety, We have reduce the number of pages in this consistency, and performance. catalogue to a minimum. Nevertheless it clearly shows you our interesting product range and Products Capricorn Scientific manufactures and provides a complete range of cell culture products which include: • Animal and Human Sera • Supplements • Basic and Special Reagents • Cell Culture Media and Buffer Solutions • Customization of Products In addition, Capricorn Scientific continues to expand its product range to offer novel, innovative products. We continually add products to our portfolio which have desired advantages for our customers. introduces you to the new cell culture brand Capricorn Scientific. For further and the most current information about media formulation, specifications, protocols and technical information click on www.capricorn-scientific.com 3 Content Sera Reagents Fetal Bovine Serum Bovine Serum Albumin Fetal Bovine Serum ........................................... 8 Bovine Serum Albumin, Fetal Bovine Serum Advanced ....................... 9 Fraction V, pH 7.0 ............................................ 14 Fetal Bovine Serum ES Cell Pretested .......... 9 Bovine Serum Albumin, Fatty Acid Free .....14 Fetal Bovine Serum Bovine Serum Albumin, Protease Free ..... 14 Tetracycline Negative ........................................ 9 Supplements Other Animal Sera 4 G5 Supplement .................................................15 Newborn Calf Serum ..................................... 10 N2 Supplement ............................................... 16 Calf Serum ........................................................ 10 N2 Supplement Modified ............................. 16 Adult Bovine Serum ....................................... 10 NCS21 Supplement ........................................ 17 Horse Serum ................................................... 10 NCS27 Supplement ........................................ 17 Donor Horse Serum ...................................... 10 mLIF, Recombinant ........................................ 18 Goat Serum ...................................................... 10 ITS, Insulin, Transferrin, Selenium .............. 18 Porcine Serum ................................................. 10 Hybridoma Supplement ............................... 19 Mouse Serum .................................................. 10 L-Glutamine .................................................... 19 Rat Serum ......................................................... 10 Stable Glutamine ............................................ 19 Rabbit Serum ................................................... 10 Sodium Pyruvate ............................................. 19 MEM Nonessential Amino Acids ................. 19 Human Sera Human Serum, Off the Clot ...........................11 Detachment Human Serum, Off the Clot, Type AB ........ 11 Accutase ..............................................................20 Human Serum, Converted ........................... 11 Trypsin ............................................................... 20 Human Serum, Converted, Type AB .......... 11 Antibiotics Amphotericin B Solution ............................... 21 Antibiotic/Antimycotic Solution ................... 21 Gentamicin Solution ...................................... 21 Penicillin/Streptomycin ................................. 21 Penicillin/Streptomycin, with L-Glutamine ............................................ 21 Cell Separation Media Lymphocyte Separation Medium ............... 21 Media Cryopreservation Media Classical Media FreezeMe One ................................................. 23 DMEM High Glucose (4.5 g/l) ....................... 34 FreezeMe Two ................................................. 23 DMEM Low Glucose (1 g/l) ........................... 34 DMEM/Ham’s F-12 .......................................... 34 Mycoplasma Detection & Removal EasyDetect M, PCR Detection Kit ................ 25 MycoXpert, Removal Reagent ...................... 26 Ham’s F-12 ....................................................... 34 IMDM ................................................................. 34 Leibovitz’s L-15 Medium ............................... 34 McCoy’s 5A Medium ...................................... 34 Medium 199 ..................................................... 34 Transfection MEM with Earle’s Salts ................................... 34 AlphaFectin, Transfection Reagent ............ 27 MEM Alpha Modification ............................... 34 G-418 Sulfate EvoPure ................................ 27 RPMI 1640 ........................................................ 34 ® G-418 Sulfate ................................................... 28 Blasticidin S ...................................................... 29 Hygromycin B .................................................. 29 Puromycin ........................................................ 29 Cytogenetics LymphoSelect .................................................. 30 AmnioSelect ..................................................... 30 Colcemid ........................................................... 30 Buffer & Salt Solutions Dulbecco’s PBS ................................................ 31 Hanks‘ Balanced Salts .................................... 31 HEPES Buffer Solution ................................... 31 5 6 Sera Animal Sera are the most important growth factor source and supplement in cell culture. Product reliability is crucial. Capricorn Scientific is a trustworthy partner. The huge number of different applications requires the optimum serum product. Choose between our different categories and origins. All our sera feature consistent high quality and sterility. Additional products and treatments are available on request. More information for all products can be found under www.capricorn-scientific.com 7 Fetal Bovine Serum For standard cell culture applications in academ- Our FBS is available from two different origins ic research Fetal Bovine Serum from Capricorn as well as with additional treatments such as Scientific is perfectly suitable. It is produced by heat inactivation and gamma irradiation altering certain parameters to promote optimal (on request). cell growth with low batch-to-batch variation, mainly from EU-approved material. www.capricorn-scientific.com Product 8 Vol. Cat. No. Fetal Bovine Serum, Collected in South America 100 ml FBS-12B 500 ml FBS-12A Fetal Bovine Serum, Collected in South America, Heat Inactivated 100 ml FBS-HI-12B 500 ml FBS-HI-12A Fetal Bovine Serum, Collected in USA 100 ml FBS-22B 500 ml FBS-22A Fetal Bovine Serum, Collected in USA, Heat Inactivated 100 ml FBS-HI-22B 500 ml FBS-HI-22A Fetal Bovine Serum Advanced Raw FBS is a natural product obtained from results. Capricorn Scientific’s sophisticated treat- bovine blood showing significant batch varia- ments during pooling and filtering significantly tions due to various environmental influences reduces batch-to-batch variation in FBS e.g. nutrition or health state of the animals. Advanced. Once successfully tested on a cell Batch-to-batch variations of FBS can have major line, no further batch testing is necessary. impacts on the reproducibility of scientific Applications Features • Scientific research • Minimized batch-to-batch variation • Calibration assays • A fter initial testing no further batch testing • Fermentation • Stabilizer for diagnostic kits necessary – Saves time and manpower! • No further batch reservation necessary • No synthetic additives Product Vol. Cat. No. Fetal Bovine Serum Advanced, Collected in South America 100 ml FBS-11B 500 ml FBS-11A Fetal Bovine Serum Advanced, Collected in South America, 100 ml FBS-HI-11B Heat Inactivated 500 ml FBS-HI-11A Vol. Cat. No. Fetal Bovine Serum Specials Product Fetal Bovine Serum ES Cell Pretested, USDA Approved 100 ml FBS-ES-32B 500 ml FBS-ES-32A Fetal Bovine Serum Tetraycycline Negative, Collected in South America 100 ml FBS-TET-12B 500 ml FBS-TET-12A The origins, tests and treatments below are available on request. Please do not hesitate to contact us to discuss your requirements. Additional Origins Additional Tests & Treatments • Australia • Tested according to 9CFR • USDA Approved • Gamma Irradiated • Dialysed • Charcoal Treated • IgG Stripped Sera • Suitable for all cell lines 9 Other Animal Sera Other animal sera are used in various applica- proliferate mycoplasma or viruses. Goat and tions, e.g. autologous cell culture systems or Sheep Serum are used in immunohistochemis- immunohistological methods. try and to eliminate non-specific binding Porcine serum is often used in cultures that reactions. Applications: • Autologous cell culture systems • Immunohistochemistry • Immunoblotting www.capricorn-scientific.com • Immunofluorescence 10 • Immunoprecipitation Product Vol. Cat. No. Newborn Calf Serum 100 ml NCS-1B 500 ml NCS-1A Newborn Calf Serum, Heat Inactivated 100 ml NCS-HI-1B 500 ml NCS-HI-1A Calf Serum 100 ml CS-1B 500 ml CS-1A Adult Bovine Serum 100 ml ABS-1B 500 ml ABS-1A Horse Serum 100 ml HOS-1B 500 ml HOS-1A Donor Horse Serum 100 ml DHS-1B 500 ml DHS-1A Goat Serum 100 ml GOA-1B 500 ml GOA-1A Porcine Serum 100 ml POR-1B 500 ml POR-1A Mouse Serum 100 ml MOU-1B Rat Serum 100 ml RAT-1B Rabbit Serum 100 ml RAB-1B Human Sera Human Serum Off the Clot is collected from in the presence of an anticoagulant, such as whole blood that coagulates naturally after sodium citrate. Plasma-derived serum is collection. It has not been exposed to any anti- generally more affordable and consistent than coagulants. off-the-clot products. Human Serum Off the Clot, Type AB is col- Human Serum Converted, Type AB is defibri- lected from healthy AB donors at FDA-licensed nated from source plasma AB. facilities. As a natural off-the-clot product, this material contains neither anticoagulants nor Why blood type AB? preservatives. All donors are tested for viral The Human AB+ Serum lacks antibodies against markers (HIVAg, HBsAg, HIV 1 and HIV 2) and the A and B blood type antigens. It is recom- found to be non-reactive. mended that sera containing any antibodies Human Serum Converted is produced by with the cultured cells be avoided, especially if defibrinating pooled human plasma, collected the cells are destined to be used for therapy. Product Vol. Cat. No. Human Serum, Off the Clot 100 ml HUM-8B Human Serum, Off the Clot, Type AB 100 ml HUM-9B Human Serum, Converted 100 ml HUM-1B Human Serum, Converted, Type AB 100 ml HUM-2B All Human Sera of Capricorn Scientific are intended for research use only, not for ex vivo or in vivo therapeutic uses! Sera (not just anti-ABO antibodies) that could react 11 12 Reagents Selecting the appropriate medium for your cell culture is important to ensure experimental consistency and reproducibility. The majority of cell lines grow well in basal media supplemented with serum. However, a variety of applications require further optimization of basal media with reagents, supplements, growth factors, and antibiotics. Capricorn Scientific manufactures a broad range of these supporting products, which are intensively tested for purity, toxicity and cell functionality, and help to ensure the reliability and consistency of your cell culture research. More information for all products can be found under www.capricorn-scientific.com 13 www.capricorn-scientific.com Bovine Serum Albumin 14 Bovine Serum Albumin (also known as BSA or differential solubility characteristics of plasma „Fraction V“) is a serum albumin protein derived proteins. BSA has numerous biochemical from bovine blood. It is often used as a protein applications including ELISAs (Enzyme-Linked concentration standard in lab experiments. Immunosorbent Assay), immunoblots, and The term „Fraction V“ refers to albumin being immunohistochemistry. It is also used as a the fifth fraction of the original Edwin Cohn stabilizer and cell growth supplement in cell purification methodology that makes use of the and microbial cultures. Product Vol. Cat. No. Bovine Serum Albumin, Fraction V, pH 7.0, Origin USA 50 g BSA-1U 100 g BSA-1S 250 g BSA-1V 500 g BSA-1T 50 g BSA-FAF-1U 100 g BSA-FAF-1S 50 g BSA-PF-1U 100 g BSA-PF-1S Bovine Serum Albumin, Fatty Acid Free, Origin USA Bovine Serum Albumin, Protease Free, Origin USA Supplements Capricorn Scientific offers chemically defined supplements for a variety of cell culture applications. These include growth factors for serum-free cultivation, to support the growth of special cell types, or to reduce the use of FBS. G5 Supplement is a serum-free chemically based on Bottenstein’s G5 formulation. defined supplement for cultivation of primary G5 Supplement may be used with DMEM, glial cells or tumor cells of astrocytic phenotype DMEM/Ham’s F-12, or other basal media. Reagents G5 Supplement Applications G5 Supplement can be used in combination with NCS21 or NCS27 Supplement for: • D ifferentiation of ES cells into neuron lineage (neuron and astrocytes) • Differentiation of neuronal stem cells into astrocytes and neurons • O ptimal growth and long-term survival of rat hippocampal neurons (fetal and adult) • S urvival of neurons from embryonic rat stria- tum, substantia nigra, septum and cortex, and neonatal rat cerebellum (fetal and adult) Product Vol. Cat. No. G5 Supplement (100x), Serum-free 1 ml G5-L 15 N2 Supplement N2 Supplement is a serum-free chemically for the survival and expression of post-mitotic defined supplement based on Bottenstein’s neurons in primary cultures from both the N2 formulation. It is recommended for the peripheral nervous system (PNS) and the cen- growth and expression of neuroblastomas and tral nervous system (CNS). Applications N2 Supplement can be used in combination with NCS21 or NCS27 Supplement for: • D ifferentiation of ES cells into neuron lineage www.capricorn-scientific.com (neuron and astrocytes) 16 • D ifferentiation of neural stem cells into astrocytes and neurons • O ptimal for serum free growth for neuroblastomas Product Vol. Cat. No. N2 Supplement (100x), Serum-free 5 ml N2-K N2 Supplement Modified N2 Supplement Modified is a serum-free chemi- vous system (PNS) and the central nervous sys- cally defined supplement for the growth and tem (CNS). It is a modified formulation of expression of neuroblastomas and for the sur- Bottenstein’s N2 formulation. It features a vival and expression of post-mitotic neurons in reduced transferrin concentration which sup- primary cultures from both the peripheral ner- ports the growth of a great variety of neural cells. Product Vol. Cat. No. N2 Supplement Modified (100x), Serum-free 5 ml N2M-K NCS21 Supplement, Serum-free NCS21 Supplement is a serum-free supplement hippocampal and other neurons of the central for neuronal cell cultures. It is an optimized and nervous (CNS) and peripheral nervous system modified formulation of B27 Supplement (PNS). It is chemically defined and contains vita- (B27® is a registered trade mark of Life Tech- mins, hormones and other growth factors includ- nologies Corporation). NCS21 Supplement is ing insulin, human transferrin, catalase, antioxi- suitable for the long-term growth and viability of dants and fatty acids. ® Applications • D ifferentiation of ES cells into neuron lineage (neuron and astrocytes) • D ifferentiation of neuronal stem cells into astrocytes and neurons • O ptimal growth and long-term survival of rat hippocampal neurons (fetal and adult) • S urvival of neurons from embryonic rat stria- tum, substantia nigra, septum and cortex, and Product NCS21 Supplement (50x), Serum-free Vol. Cat. No. 10 ml C21-H NCS27 Supplement, Serum-free 17 NCS27 Supplement is a serum-free supplement NCS27 Supplement is chemically defined and and is suitable for use in most neuronal cell contains vitamins, hormones and other growth applications. It is based upon a formulation factors including insulin, human transferrin, cat- published by Brewer et al. (1989). alase, antioxidants and fatty acids. Applications • D ifferentiation of ES cells into neuron lineage (neuron and astrocytes) • D ifferentiation of neuronal stem cells into astrocytes and neurons • O ptimal growth and long-term survival of rat hippocampal neurons (fetal and adult) • S urvival of neurons from embryonic rat stria- tum, substantia nigra, septum and cortex, and neonatal rat cerebellum (fetal and adult) Product NCS27 Supplement (50x), Serum-free Reagents neonatal rat cerebellum (fetal and adult) Vol. Cat. No. 10 ml C27-H mLIF, Recombinant, for Murine Embryonic Cells Leukemia Inhibitory Factor (LIF) is a lymphoid sterile PBS with 0.5 % BSA. The activity of mLIF growth factor that promotes long-term cell cul- is higher than 1 x 108 units/mg. 1 ml mLIF is ture of primary cells. Recombinant mouse LIF equivalent to 107 units and sufficient for supple- produced in E. coli is a single, non-glycosylated, menting 10 liter culture medium. www.capricorn-scientific.com polypeptide chain. mLIF is reconstituted in 18 Product Vol. Cat. No. mLIF, Recombinant, for Murine Embryonic Cells 1 ml LIF-L Insulin Transferrin Selenium (ITS) ITS (Insulin, Transferrin, Selenium) is a chemical- tially reduces the FBS requirement for routine ly defined supplement for a variety of cell cul- maintenance of many cell lines such as CHO, ture applications and contains components Hybridoma, MDCK and Vero. ITS can also be which are required for optimum performance used as a supplement in embryonic stem cell of serum-free media. Supplementation of ITS to culture. different conventional nutrient media substanProduct ITS (100x), Insulin, Transferrin, Selenium Vol. Cat. No. 10 ml ITS-H Hybridoma Supplement To support hybridoma development and to occurring by the use of feeder cells may achieve an optimal cell density as well as clon- include: overgrowth of newly formed hybrid- ing efficiency, growth factors and serum (10 to omas, source of contamination, competition 20 %) are required. Hybridoma Supplement is for nutrients, and variations in growth factor a chemically defined growth supplement con- concentrations. taining insulin, ethanolamine, hydrocortisone, retinoic acid, linoleic acid and other growth Hybridoma Supplement can be used in culture promoting factors. It supports the growth of media under serum-free conditions. The low hybridoma in the same manner feeder cells protein content facilitates the isolation and have been used before. The disadvantages purification of produced antibodies. Vol. Cat. No. 50 ml HCS-F Vol. Cat. No. L-Glutamine (200 mM) 100 ml GLN-B Stable Glutamine (200 mM) 100 ml STA-B Sodium Pyruvate (100 mM) 100 ml NPY-B MEM Nonessential Amino Acids (100x) 100 ml NEAA-B Hybridoma Supplement (10x), Serum-free Reagents Product Other Supplements Product 19 Detachment www.capricorn-scientific.com Accutase – The Original A huge range of “Accutase-like” products are The cell surface epitopes remain intact so that available on the market. Capricorn Scientific experiments will not be marred and the cells distributes the original Accutase, which guaran- can faster and easily reattach. The product is of tees gentle and effective detachment of adher- non-mammalian origin which can be of impor- ent cells by promoting maximum cell viability. tance for some applications. Features • L ess aggressive than trypsin • N o neutralization required • M aximum cell viability, cell recovery and • S uitable for a great variety of cells faster cell reattachment times Product including hESC Vol. Cat. No. 100 ml ACC-1B Vol. Cat. No. Trypsin-EDTA (0.05 %) in DPBS (1x) 100 ml TRY-1B Trypsin-EDTA (0.5 %) in DPBS (10x) 100 ml TRY-1B10 Trypsin (0.25 %) in DPBS (1x) 100 ml TRY-2B Trypsin (2.5 %) in DPBS (10x) 100 ml TRY-2B10 Accutase Cell Detachment Solution 20 Trypsin Product Antibiotics Antibiotics and Antimycotics are helpful reagents to prevent bacterial or fungal contamination in cell culture. Vol. Cat. No. Amphotericin B Solution (250 µg/ml) 100 ml AMP-B Antibiotic/Antimycotic Solution (100x) 100 ml AAS-B Gentamicin Solution (10 mg/ml) 100 ml GEN-10B Gentamicin Solution (50 mg/ml) 100 ml GEN-50B Penicillin/Streptomycin (100x) 100 ml PS-B Penicillin/Streptomycin (100x), with L-Glutamine 100 ml PSG-B Vol. Cat. No. Lymphocyte Separation Medium, Density 1.077 g/ml 100 ml LSM-B 500 ml LSM-A Reagents Product Cell Separation Product 21 www.capricorn-scientific.com 22 Cryopreservation Safely cryopreserving cells is important for storing cells for future studies and ensures that you have back-up cells in case of contamination and loss of cell supply. The new series of easy-to-use freezing media from Capricorn Scientific offers high cell viability in serum and serum-free formulations to successfully cryopreserve the most sensitive and valuable mammalian cells. FreezeMe One Cryopreservation Medium with FBS FreezeMe One is our new optimized ready-to- the highest cell viability (> 93 %) and is suitable use medium containing FBS, US Origin and the for primary cells, cell lines and applications commonly used and most efficient cryo-pro- such as cell banking and stem cell storage. tectant DMSO. FreezeMe One shows levels of Product FreezeMe One, Cryopreservation Medium with Fetal Bovine Serum Vol. Cat. No. 50 ml FM1-F Cryopreservation Medium, Serum-free FreezeMe Two, our new serum-free cell freezing levels of viability that are comparable to cells medium (ADCF), is a sterile, ready-to-use preserved in DMSO and FBS. FreezeMe Two medium suitable for the cryopreservation of can be used for both cells cultured in adherent and suspension cells. serum-supplemented growth medium as well Cells cryopreserved using FreezeMe Two show as cells grown under serum-free conditions. Reagents FreezeMe Two 23 Applications • C ryopreservation of industrial cell lines • Industrial cell banking • Stem cell storage • H uman Embryonic Stem Cells and Induced Pluripotent Stem Cells Product FreezeMe Two, Cryopreservation Medium, Serum-free Vol. Cat. No. 50 ml FM2-F www.capricorn-scientific.com 24 Mycoplasma Detection & Removal Mycoplasma is a prokaryotic organism that is a frequent contaminant of mammalian cell cultures. It can alter cell physiology and impair growth which may lead to false results in your experimental work. Because of their small size, Mycoplasmas can pass through filters used to prevent bacterial and fungal contamination and potentially spread to all cultures in a laboratory. Therefore, it is recommended to test all new mammalian cell cultures for the presence of Mycoplasma. Capricorn Scientific provides a simple, rapid and reliable PCR-based test for the detection of mycoplasma contamination in cell cultures. EasyDetect M, Mycoplasma PCR Detection Kit EasyDetect M is a novel mycoplasma PCR-grade water (included in the kit) and add PCR detection kit for maximum convenience in the DNA to be analyzed. Each master-mix mycoplasma detection. The kit contains lyophilisate contains a broad-range PCR primer PCR cups filled with a ready-to-use lyophilized mix, a hot-start thermostable DNA polymerase master mix. Before amplification it is only nec- and buffer. A separate DNA control can be used essary to rehydrate the lyophilisates with as positive and spike control. Features • High Stability Detection of <50 cfu/ml infected cells or Lyophilization ensures stability of enzymes 100 DNA molecules (corresponds with for long term storage at +2 to +8°C. Storage 400 attogram DNA) at -20°C is not required • Broad Detection Range • High Safety Detection of all mycoplasma strains listed Lyophilized PCR master mixes reduce cross in the European Pharmacopoeia and more contamination because no further handling than 100 species other than addition of test DNA samples is necessary 25 • Maximum Convenience Easy-to-use: All components for PCR detection are included; only rehydration of lyophilisates and addition of test DNA necessary Product Vol. Cat. No. EasyDetect M, Mycoplasma PCR Detection Kit 24 assays MYD-24 48 assays MYD-48 96 assays MYD-96 Cell cultures found contaminated can be effectively treated with MycoXpert a well established mycoplasma removal reagent. Reagents • High Sensitivity MycoXpert, Mycoplasma Removal Reagent MycoXpert is a high efficient antibiotic, active at the DNA replication machinery, thus inhibiting low concentration for a broad range of myco- mycoplasma growth. plasma subspecies. It acts by interfering with Applications Features • Mycoplasma infected established cell lines • Effective mycoplasma removal • Decontamination of primary material • E ffective against a broad range www.capricorn-scientific.com • Prophylaxis for cell culture 26 of mycoplasma • D oes not negatively affect eukaryotic cell proliferation • Easy-to-use Product MycoXpert, Mycoplasma Removal Reagent (50x) Vol. Cat. No. 100 ml MYX-B Transfection AlphaFectin, Transfection Reagent AlphaFectin contains a new blend of polycationic bind higher amounts of nucleic acids and to lipids for an effective transfection of DNA or RNA form stable lipoplexes. The lipoplex-supporting in eukaryotic cells. It is suitable for adherent and supplement increases nucleic acid complex for- suspension cells and supports a transient and mation and facilitates the membrane transfer stable expression. and release into the cell. Therefore, higher The unique polycationic surface structure with amounts of nucleic acids are transfected into the dendrimere-like branches enables AlphaFectin to cells resulting in higher transfection efficiencies. Reagents Features • High transfection efficiency • Low cytotoxicity • Transfection in presence or absence of serum • Easy protocol Product AlphaFectin, Transfection Reagent and Supplement Vol. Cat. No. 1 ml + 50 ml AFE-L G-418 Sulfate EvoPure® – for the Selection of Your Successful Clone The purity of commercially available G-418 available with a purity of >99.5 %. This ultra- ranges from approx. 60 % to 90 %. Recent stud- pure G-418 allows higher selection concentra- ies have shown that the impurities in G-418 tions for rapid elimination of sensitive cells. (mostly Gentamicin derivates) have very high Therefore the selection process can be signifi- toxicities to several G-418 resistant mammalian cantly shortened from three weeks to only cells. The toxicity of these impurities limits one week! G-418 EvoPure® shows much lower the applicable working concentration of G-418. batch-to-batch variations and thus saves By using today‘s best separation technologies, on optimization time and gives more consistent our G-418 EvoPure® is the purest G-418 results. Advantages • Higher viability of resistant cells • More accurate and consistent results • F aster selection process and faster • Shorter or no re-optimization required post-transfection recovery time 27 G-418 Sulfate, Selection Antibiotic G-418 Sulfate is an aminoglycoside antibiotic with the same active concentration. You and commonly used in plant and mammalian gain maximum convenience: calculating, cell culture as a selectable marker. Capricorn’s weighing, filtering and aliquoting are no longer G-418 Sulfate Solution features a 100% activity. necessary. www.capricorn-scientific.com In contrast to other commercially available 28 Just like most other high potent reagents selection antibiotics Capricorn Scientific does for cell culture G-418 Solution should also be not solely produce its product according to the stored at ≤-15 °C in order to ensure maximum weight, but also based on the specific activity activity. To avoid repeated freeze-thaw cycles, of the powder. This provides you with consis- Capricorn has introduced a new convenient tent biological potency and each lot is supplied pack size of 10 x 10 ml. Product Vol. Cat. No. 5 g G418EP-Q 10 ml G418EP-H 100 ml G418EP-B 10 ml G418-H 10 x 10 ml G418-10X 100 ml G418-B 5 g G418-Q 10 g G418-R G-418 Sulfate EvoPure Powder ® G-418 Sulfate EvoPure® Solution, 100 % Activity (50 mg/ml) G-418 Sulfate Solution, 100 % Activity (50 mg/ml) G-418 Sulfate Powder EvoPure® is a registered trademark of TOKU-E Other Selection Antibiotics Blasticidin Puromycin Blasticidin is a nucleoside antibiotic and used Puromycin is an aminoglycoside antibiotic for the selection of cells transfected with plas- used for the selection of mammalian cell lines mids carrying the pac, bls, bsr or bsd genes. expressing the puromycin resistance gene. Blasticidin works by inhibiting the protein syn- It inhibits protein synthesis in gram positive thesis in many bacteria, yeast and animal cells. bacteria, various animal and insect cells. Hygromycin B Hygromycin B is an aminoglycoside antibiotic with a broad spectrum of activity. It is effective against most bacteria, fungi and higher eukarytransient and/or stable transfected cells carrying the hph resistant gene. Product Blasticidin S Powder Hygromycin B Solution (50 mg/ml) Puromycin Powder Vol. Cat. No. 50 mg BLA-5X 100 mg BLA-1X 10 ml HYG-H 100 mg PUR-1X Reagents otes. Hygromycin B is suitable for use with 29 Cytogenetics Capricorn Scientific offers a range of reliable cytogenetics products for chromosome karyotyping. This includes media for cultivation of amniotic fluid and chorionic villi cells, peripheral blood lymphocytes, as well as related reagents. Time is precious! Do you wish to deliver your karyotyping analysis www.capricorn-scientific.com in the fastest and most reliable way possible? Our amnion 30 medium, AmnioSelect has proven to be one of the fastest media with regards to the diagnostics of chromosomal aberrations in the market. AmnioSelect LymphoSelect AmnioSelect is a ready-to-use medium, specifi- LymphoSelect is specifically optimized for short- cally optimized for the primary culture of term cultivation of peripheral blood lympho- human amniotic fluid cells and chorionic villi cytes for chromosome analysis. It is ready to (CV) samples. It has been designed for rapid use after thawing and Phytohaemagglutinin M cell growth and fast chromosome karyotyping. (PHA-M) supplementation. Colcemid Colcemid is used in chromosome analysis during lymphocyte karyotyping and amniotic fluid cell chromosome analysis, and in cell synchronization. It arrests mitotic cultured cells in metaphase. Product Vol. Cat. No. LymphoSelect 100 ml PBL-1B AmnioSelect 100 ml Tri-21B 10 ml COL-H Colcemid Solution (10 µg/ml) in DPBS Buffer and Salt Solutions are used in many dif- quality water together with excellent raw ferent applications, predominantly as diluting, materials guarantees that our customers rinsing and reference solutions. The use of high receive sterile buffers of the highest quality. Product Vol. Cat. No. Dulbecco‘s PBS (1x), w/o Ca & Mg, w/o Phenol Red 500 ml PBS-1A Dulbecco‘s PBS (10x), w/o Ca & Mg, w/o Phenol Red 500 ml PBS-10XA Dulbecco‘s PBS (1x), with Ca & Mg, w/o Phenol Red 500 ml PBS-2A Hanks‘ Balanced Salts (1x), with Ca & Mg, w/o Phenol Red 500 ml HBSS-1A Hanks‘ Balanced Salts (1x), w/o Ca & Mg, w/o Phenol Red 500 ml HBSS-2A Hanks‘ Balanced Salts (1x), w/o Ca & Mg, with Phenol Red 500 ml HBSS-3A HEPES Buffer Solution (1 M) 100 ml HEP-B Reagents Buffer & Salt Solutions 31 32 Media The culture medium is the most important component of the culture environment, because it provides the necessary nutrients, growth factors, and hormones for cell growth. In addition, it regulates the pH and the osmotic pressure of the culture. The liquid media from Capricorn are suitable for a variety of cell culture applications. Each of our culture media is manufactured according to the originally published formula or modifications essential to the con sistent performance and stability of the culture medium. Our media are produced under strict quality standards and production procedures, using only high quality raw materials. More information for all products can be found under www.capricorn-scientific.com 33 Classical Media Product DMEM High Glucose (4.5 g/l), with L-Glutamine Vol. Cat. No. 500 ml DMEM-HA DMEM High Glucose (4.5 g/l), with Stable Glutamine, with 25 mM HEPES 500 ml DMEM-HHSTA www.capricorn-scientific.com DMEM High Glucose (4.5 g/l), with L-Glutamine, with Sodium Pyruvate 34 500 ml DMEM-HPA DMEM High Glucose (4.5 g/l), with Stable Glutamine, with Sodium Pyruvate 500 ml DMEM-HPSTA DMEM High Glucose (4.5 g/l), w/o L-Glutamine, with Sodium Pyruvate 500 ml DMEM-HPXA DMEM High Glucose (4.5 g/l), w/o L-Glutamine 500 ml DMEM-HXA DMEM High Glucose (4.5 g/l), w/o L-Glutamine, w/o Phenol Red 500 ml DMEM-HXRXA DMEM Low Glucose (1 g/l), with L-Glutamine, with Sodium Pyruvate 500 ml DMEM-LPA DMEM Low Glucose (1 g/l), with Stable Glutamine, with Sodium Pyruvate 500 ml DMEM-LPSTA DMEM Low Glucose (1 g/l), w/o L-Glutamine, with Sodium Pyruvate 500 ml DMEM-LPXA DMEM/Ham’s F-12, with L-Glutamine, with 15 mM HEPES 500 ml DMEM-12-HA DMEM/Ham’s F-12, with Stable Glutamine, with 15 mM HEPES 500 ml DMEM-12-HSTA DMEM/Ham’s F-12, w/o L-Glutamine 500 ml DMEM-12-XA Ham’s F-12, with L-Glutamine 500 ml HAM-12-A IMDM, with L-Glutamine, without Supplements 500 ml IMDM-A Leibovitz’s L-15 Medium, w/o L-Glutamine 500 ml L15-XA McCoy’s 5A Medium, with L-Glutamine 500 ml MCC-A Medium 199, with Earle‘s Salts, with L-Glutamine 500 ml M199-A MEM with Earle’s Salts, with L-Glutamine 500 ml MEM-A MEM with Earle’s Salts, with Stable Glutamine 500 ml MEM-STA MEM with Earle’s Salts, w/o L-Glutamine 500 ml MEM-XA MEM Alpha Modification, with L-Glutamine, with Nucleosides 500 ml MEMA-RA MEM Alpha Modification, with L-Glutamine, w/o Nucleosides 500 ml MEMA-XRA RPMI 1640, with L-Glutamine 500 ml RPMI-A RPMI 1640, with Stable Glutamine 500 ml RPMI-STA RPMI 1640, with L-Glutamine, with 25 mM HEPES 500 ml RPMI-HA RPMI 1640, w/o L-Glutamine 500 ml RPMI-XA RPMI 1640, w/o L-Glutamine, with 25 mM HEPES 500 ml RPMI-HXA RPMI 1640, w/o L-Glutamine, w/o Phenol Red 500 ml RPMI-XRXA 35 Media CSM0001/ 0314 / 4 Capricorn Scientific GmbH Auf der Lette 13 A 35085 Ebsdorfergrund Germany Phone: +49 6424 944 64-0 Fax: +49 6424 944 64-20 [email protected]
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