ANAEROBIC JAR SYSTEM

ANAEROBIC JAR
SYSTEM
TBL 001
USER’S MANUAL
INTRODUCTION
Titan Biotech Ltd has designed and developed a convenient, rapid & cost effective Anaerobic
Jar System (TBL 001) and anaerobic system accessories. The anaerobic accessories include
anaerobe indicator tablet (TBL 001C) and anaerobe gas pack (TBL 001D). This system is
applied in microbiological laboratories for the isolation/culturing of anaerobic and
microaerophilic microorganisms. The Anaerobic Jar System provides oxygen free environment
by two methods. Either by using chemical gas pack (anaerobe gas pack) or by manually
evacuating the jars with a vacuum pump and flushing with gas afterwards .The jars are made
of transparent polycarbonate. The tough metal lid is equipped with two corner valves for
vacuum hoses (5 mm i.d.) and with manometer for exact control of the vacuum or
overpressure from -30 to 30 psi.
HOW DOES IT WORKS:
This system consists of a polycarbonate jar, a lid with a gasket to prevent air flow, an
anaerobe indicator tablet, an anaerobe gas pack (pouch containing sodium borohydride,
sodium bicarbonate, citric acid) and a pouch of palladium catalyst.
When water is added to the anaerobe gas pack, the sodium borohydride, sodium bicarbonate,
and citric acid react to form hydrogen and carbon dioxide gases. The palladium catalyst
catalyzes a reaction between the hydrogen and the oxygen within the jar; this reaction creates
water, which forms as condensation on the inside of the jar. Anaerobe indicator tablet contains
Methylene blue, which remains blue in the presence of oxygen but turns to colorless in an
anaerobic environment. When the hydrogen combines to free oxygen present inside the jar, it
is converted to water and condensation forms on the side of the jar, the indicator tablet will
turn from blue to white. The concentration of CO2 reaches to 10% (v/v) inside the jar, favoring
the growth of anaerobes.
PRE-INSTALLATION REQUISITIES
1. Vacuum Pump :
According to the requirement of the test, a Vacuum Pump should be available at the site for
the Installation & Demonstration of the Anaerobic Jar System.
2. Gases :
Sources of gases like hydrogen, nitrogen, carbon dioxide etc. should be available in the
cylinders, to create the desired anaerobic atmosphere.
SPECIFICATIONS
ANAEROBIC JAR SYSTEM
(A system to provide anaerobic environment to bacteria)
It is a transparent, unbreakable, polycarbonate Jar of 3.5 liter capacity with a steady Aluminum
lid & screw down clamp. The Lid is lined on the inner side with a Rubber Gasket for a tight fit &
to prevent any leakages. The system has an in-built Safety Valve, Vacuum valve & Pressure
valve to create vacuum & introduce gases into the Jar. It also has a 2 way pressure Gauge
system to monitor the vacuum & pressure.
Specification:
Detector
Range
Weight of jar
Capacity
:
:
:
:
Two way Pressure Gauge system.
-30 psi (-1 kg/cm2) to +30 psi (2.1 kg/cm2)
2.20 kg ± 50 gm.
3.5 Ltrs
Accesories:
Important Features Includes:




A stainless steel Petri-Plate carrier to stack 10 Petri plates inside the Jar.
Palladium Catalyst Pouch
Five Anaerobic Gas Pak pouches, as a Start-up Kit.
Anaerobe indicator tablet.
OPERATING INSTURCTIONS
[WHEN USING THE ANAEROBE GAS PACK]
Note:
Before use, It is advisable to check the rubber ring is correctly sealed and vacuum relief
screw is in closed position.
1.
Place the inoculated Petri plates in the plate carrier. Disposable Plastic Petri Plates should
be of the vented variety to aid gas transfer between the interior & exterior of the dishes.
2.
Lower the dish carrier into the Polycarbonate Jar & place the anaerobic indicator without
cap at the base. Ensure that the anaerobic indicator is pale pink/colorless.
3.
Cut off the corner of the Anaerobe Gas Pack pouch and immediately place in the lower clip
of the Petri plate carrier. Add 2 ml of water to the pouch. Avoid folding or crushing the Gas
Pack.
4.
Having inserted the sachet in the carrier, place the lid on the Jar and ensure that the
Rubber ring is correctly placed, evenly pressed against the flange as a secure fit. Move
the clamp horizontally over the top of the Lid, so that the screw thread is positioned at the
centre of the Lid. Close the Lid tightly with the Screw-down Clamp.
5.
After 2-3 hours, the anaerobic indicator may turn to colorless from original blue color.
6.
Remove Jar after the appropriate incubation period & open lid by carefully screwing-up the
knurled wheel until loose enough to release the lid from the Jar base.
7.
Excessive downward pressure on the clips should be avoided.
8.
After incubation the anaerobic indicator tablet may be discarded. The exhausted anaerobe
gas pack should be removed without spilling the contents. The solution left in the sachet is
mildly acidic & may be poured into the sink & flushed with running water. The empty
sachet can be discarded.
Cleaning and Disinfection
Disposable gloves should be worn throughout the operation/handling. Internal surface should be
cleaned with anti-bacterial dish soap and hot water.
Using Sodium Hypochlorite, Phenolic Compounds, Methanol and chloroform as disinfectant may
damage the surface of the jar.
OPERATING INSTURCTIONS
[WHEN USING THE EVACUATION / REPLACEMENT TECHNIQUE]
1.
Place the inoculated Petri plates in the carrier. Disposable plastic Petri dishes should be of
the vented variety to aid gas transfer between the interior & exterior of the dishes.
2.
Lower the dish carrier into the Polycarbonate Jar & place the anaerobic indicator at the
base.
3.
Place the lid on the Jar. Evenly press so as to secure fit. Move the clamp horizontally with
the top of the Lid, slightly inclined towards the gauge & safety valve, so that the screw
thread passed between the two valves to the location point at the centre of the Lid tightly
with the help of the Screw-down Clamp.
4.
A Vacuum pump has to be used for the evacuation/replacement technique. To enable a
vacuum to be created into the Jar, the tubing of the vacuum pump is fitted to the valve to
create a vacuum. The pressure valve is used to introduce the required gases.
5.
To create vacuum, open the upper knob of the valve and evacuate the system to about 8
-10 psi. The knurled wheel on the Clamp must not be further tightened if it appears loose.
6.
After use, close the upper knob of the valve and remove the vacuum pump tubing.
Observe the Pressure Gauge. A leaking jar will be detected at this stage because the
vacuum reading will not remain constant.
7.
To add the desired gases, connect the gas supply tubing to the Pressure valve of the Jar
and open the upper knob of the valve. Run the gas mixture into the jar until the pressure is
on slight positive side.
8.
Incubate the jar at desired time & temperature. The Anaerobic Indicator will change in
color. This will give the visual indication of anaerobiosis.
9.
After incubation the anaerobic indicator should be discarded.
PACKING LIST
TBL 001
1.
Manual
1 Copy
2.
Polycarbonate Jar
1 No.
3.
Petri Plate Carrier
1 No.
4.
Anaerobe Indicator Tablet
-
5.
Anaerobe Gas Pack
1 Pack X 5 sachets
5.
Palladium Catalyst Pouch
1 No.

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