2 Fakultas Kedokteran Gigi Departemen Periodonsia Tahun 2014

2
Fakultas Kedokteran Gigi
Departemen Periodonsia
Tahun 2014
Hazwani Izyan Bt Azahar
Efektivitas Ekstrak Teh Hijau (Camellia sinensis) terhadap Pertumbuhan Bakteri
Porphyromonas gingivalis secara In Vitro
x + 31 halaman
Penyakit periodontal dikaitkan dengan keberadaan bakteri negatif Gramm
yang menyebabkan kehilangan perlekatan jaringan periodontal tulang alveolar
yang mendukung gigi. Porphyromonas gingivalis adalah salah satu patogen utama
pada penyakit periodontal dan berada pada permukaan biofilm subgingiva dan
berkolonisasi di mukosa mulut. Teh hijau mengandung kadar polifenol yang
tinggi terutama catechin dan bersifat antioksidan, antikarsinogenik dan bersifat
hipolipidemik. Tujuan dari penelitian ini adalah untuk melihat efektivitas ekstrak
teh hijau terhadap bakteri Porphyromonas gingivalis dengan memperhatikan nilai
Kadar Hambat Minimum (KHM) dan Kadar Bunuh Minimum (KBM).
Daun teh hijau sebanyak ±400 gram dikeringkan dan dihaluskan,
kemudian diekstraksi dengan pelarut etanol 70% sehingga diperoleh ±45 gram
ekstrak kental teh hijau. Penentuan KHM dilakukan dengan metode dilusi yaitu
ekstrak kental teh hijau disuspensikan dengan Tryptic Soy Broth (TSB) dan
dilakukan pengenceran ganda sehingga diperoleh konsentrasi 100%, 50%, 25%,
12,5% dan 6,25% yang masing-masing terdiri dari 5 sampel. Masing-masing
konsentrasi tersebut diambil sebanyak 1 ml, ditambahkan 1ml suspensi bakteri,
divorteks dan diinkubasi 37 oC selama 24 jam pada inkubator CO2. Kekeruhan
diamati dan dibandingkan dengan kontrol Mc Farland untuk menentukan KHM.
Kemudian tiap kelompok dicampur menggunakan vorteks dan diambil 50 l,
diteteskan ke Tryptic Soy Agar (TSA), direplikasi 5 petri, didiamkan 15-20 menit
lalu diinkubasi 37 oC selama 24 jam pada inkubator CO2. Perhitungan jumlah
koloni bakteri dilakukan dengan metode Drop Plate Mills Mesra untuk
menentukan KBM.
3
Hasil penelitian menunjukkan bahwa ekstrak teh hijau mempunyai efek
antibakteri terhadap Porphyromonas gingivalis pada konsentrasi terendah, 6,25%
masih dapat membunuh bakteri dengan hasil perhitungan koloni 0 CFU/ml.
Daftar Rujukan : 22 (2005-2013)
4
Faculty of Dentistry
Department of Periodontology
Year 2014
Hazwani Izyan Bt Azahar
The Effectiveness of Green Tea Extract (Camellia sinensis) Towards Bacteria
Porphyromonas gingivalis : In Vitro Study
x + 31 pages
Periodontal disease is related with Gramm negative bacteria that caused
loss periodontal attachment of the alveolar bone which supports teeth.
Porphyromonaas gingivalis is one of the main pathogen that leads to periodontal
disease and located on biofilm of the subgingiva surface and colonized in the
mucosa of the mouth. Green tea contains polyphenol substance especially
catechin which act as antioxidant, anticarcinogenic and hypolipidemic. The
purpose of this study is to observe the effectiveness of the green tea extract
towards bacteria Porphyromonas gingivalis by using Minimum Inhibitory
Concentration (MIC) and Minimum Bactericidal Concentration (MBC).
Green tea leaves was hand picked ±400 grams, dried and was blendered
until in the form of powder, later the extraction was taken using percolation
method using ethanol 70% as solvent until ±45 grams of thick green tea extract
was formed. Minimum Inhibitory Concentration (MIC) was obtained using
dilution method which green tea extract was suspended with Tryptic Soy Broth
(TSB) and double dilution method was used until 5 samples of each concentration
between 100%, 50%, 25%, 12,5% and 6,25% was formed. 1 ml from each
concentration was taken, 1 ml bacteria suspension was added, mixed and
incubated 37 oC for 24 hours in CO2 incubator. The cloudiness of each samples
was observed and compared with Mc Farland control for determination of
Minimum Inhibitory Concentration (MIC). Later on every concentration from
previous test tube was mixed using vortex and 50 l was taken, and been dropped
into Tryptic Soy Agar (TSA), 5 petries replication was formed and let it set for
about 15-20 minutes and incubated 37 oC for 24 hours in CO2 incubator. The
5
calculation of the total bacteria colony using Drop Plate Mills Mesra method for
Minimum Bactericidal Concentration (MBC).
The results shown that green tea extract has anti bacterial effect on
Porphyromonas gingivalis and eventhough in the lowest concentration which is
6,25%, the availability to kill bacteria with total count 0 CFU/ml.
References : 22 (2005-2013)

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