CHEMISTRY 573 / BMMB 573 NMR Spectroscopy for Synthetic and Biological Chemistry SPRING SEMESTER 2014 Instructor Office Hours Class Meetings Home Page Scott A. Showalter 106 Chemistry Building By appointment 865-2318; [email protected] MWF 11:15 – 12:05 005 Life Sciences Building http://research.chem.psu.edu/sasgroup/chem573/index.html Prerequisite It is assumed that you have a strong undergraduate background in physical chemistry. Undergraduate students are welcome. None required. Suggested textbook: Spin Dynamics by Malcolm Levitt Other textbooks that may be helpful: Protein NMR Spectroscopy by Cavanagh, et al. Textbook Exams Grading There will be two take home examinations during the semester, with one due prior to spring break and the other during the finals period. Your course grade will be based on homework, examination scores, and literature presentations. Tentative breakdown is as follows: Midterm Exam Final Exam Homework Presentation 25% 25% 25% 25% NOTE: Any assignment not completed and turned in on time, without prior permission from the instructor, will receive zero credit. Academic Integrity All Penn State policies regarding ethics and honorable behavior apply to this course. Possession of unauthorized notes during exams, alteration of examinations and homework turned in for regrading, and other forms of cheating, such as plagiarism, are serious violations of ethics. Matters of academic dishonesty will be turned over to the University disciplinary system and may result in the failing of the course. See (http://www.science.psu.edu/academic/Integrity/index.html and http://www.psu.edu/ufs/policies for further details. Disability Penn State welcomes students with disabilities into the University's educational programs. Every Penn State campus has an office for students with disabilities. The Office for Disability Services (ODS) Web site provides contact information for every Penn State campus: http://equity.psu.edu/ods/dcl. For further information, please visit the Office for Disability Services Web site: http://equity.psu.edu/ods. CHEMISTRY 573 / BMMB 573 COURSE CONTENT AND SYLLABUS SPRING SEMESTER 2014 Course Goals The theory and methods of NMR spectroscopy will be covered in a survey format. The goal is to prepare both synthetic and biological chemists to understand contemporary NMR experiments and literature. Coupled with appropriate laboratory training (which is not a part of this course), students should be well prepared to apply modern NMR experiments within their individual research programs. Course Objectives By the end of the semester, CHEM 573 students should be able to: • Describe the phenomenon of nuclear magnetic resonance, drawing from both classical and quantum theory. • Identify contexts in their research program where NMR is an appropriate method to generate key data or test mechanistic and/or chemical hypotheses. • Determine and explain the expected outcome of using nearly any pulse sequence found in the libraries built into modern NMR spectrometers (such as those in the Chemistry NMR Facility). • Evaluate and critique the NMR experimental design and data analysis found in papers selected from the primary literature, particularly as it relates to their own research projects. Preliminary Schedule Week 1: General NMR Theory Week 2: The NMR Spectrometer Week 3: Quantum Mechanical Description of NMR Week 4: Theoretical Description of NMR Observables Week 5: Introduction to Mulitdimensional NMR Week 6: Homonuclear NMR Spectroscopy in Two Dimensions Week 7: Solvent Suppression and Gradient Enhanced Spectroscopy Week 8: Heteronuclear NMR Spectroscopy in Two Dimensions Exam 1 Due Week 9: Week 10: Week 11: Week 12: Week 13: Week 14: Week15: Introduction to Macromolecular NMR Macromolecular Chemical Shift Assignment Carbon Detected NMR Spectroscopy Structure Determination Methods Spin Relaxation Theory Spin Relaxation Methods Analysis of Conformational Dynamics through NMR Relaxation Spectroscopy Exam 2 Due