PrepX™ PGM 200 DNA Library Protocol

Product Note
Advancing the science of tomorrow today.
PrepX™ PGM 200 DNA Library Protocol
✓
Library Preparation for Ion Torrent PGM
The PrepX™ PGM DNA Library Preparation Kit on the Apollo 324™ System offers complete walk-away automation of the tedious library preparation process for the Ion Torrent PGM Sequencer. Our PrepX PGM 200 DNA
Library protocol carries out end repair, adapter ligation, and bead-based size selection. After you have set up
a protocol, press RUN, then walk away. No further attention is required.
PRODUCT
APPLICATION
TECHNOLOGY
Library Prep
Fragmentation
on the Apollo 324 System
Hands-on Time: 15 minutes
Run Time: 70 minutes
Quick and easy to set up
Pre-aliquoted, color-coded reagent strips and
guided step-by-step instructions on the touchscreen enable you to easily set up a run in 15
minutes.
PCR
and
QC
Sequencing
Fast library generation with
little effort
Generate up to 40 libraries in an 8-hour workday with
only 1 hour and 15 minutes total hands-on time. No gel
cut required.
PrepX™ PGM 200 DNA Library Protocol
Product Note
B
500 ng
A
50 ng
C
5 µg
Figure 1. Bioanalyzer traces of libraries generated from (A) 50 ng, (B) 500 ng and (C) 5 µg of E. coli DNA using the PrepX PGM Library Kit and PrepX
PGM 200 protocols on the Apollo 324 System.
Table 1. Summary of sequencing quality metrics of the same E. coli samples.
Input
Total # of reads
%Q17 aligned bases
50 ng
2753302
89.2%
500 ng
38953
94.4%
5 µg
56403
93.7%
Total number
of bases
Percent
enrichment
Low quality
Consensus key
647.46
96%
7%
65
Read Length Histogram
Table 2. Summary of PGM sequencing quality metrics
from P. xanthomonas samples.
Count
Total number of bases
548.23
Number of Q17 bases
398.32
Number of Q20 bases
349.68
Total number of reads
2,938,959
Mean length
187
Longest read
394
Read Length
Figure 2. PGM read lengths histogram from libraries generated from 100 ng of P. xanthomonas DNA using the PrepX PGM 200 DNA library protocol and the PrepX PGM DNA Kit on the Apollo 324 System.
Prep X 200 DNA Library Protocol and Preparation Kit Specifications
DNA input
50 ng to 5 µg
Library Output Size
~ 300 bp (contact IntegenX about making larger size libraries)
Sequencing Chemistry
Ion PGM 200 Sequencing Kit
Number samples per run
1 to 8
Apollo 324 setup time
15 minutes
Run time
70 minutes
Kit
PrepX PGM DNA Library Kit
Catalog number
400029
7400 Paseo Padre Parkway
Fremont, CA 94555 U.S.A.
[email protected]
+1 510.651.4450
www.wafergen.com
Advancing the science of tomorrow today
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
© Copyright 2014, WaferGen Biosystems. All rights reserved. Information in this document is subject to change without notice. WaferGen Biosystems assumes no responsibility for any errors that may appear in this
document. Apollo 324, PrepX, WaferGen, WaferGen BIosystems (Design), and WaferGen Biosystems are trademarks of WaferGen Biosystems or its subsidiaries in the U.S. and/or certain other countries. All other
trademarks are the sole property of their respective owners. MKT-034

Download Report