Simonetta Pulciani - European Medicines Agency

Curriculum Vitae
PERSONAL INFORMATION
Simonetta Pulciani
WORK EXPERIENCE
May 2008–Present
Senior investigator
Istituto Superiore di Sanità, (Italy)
Senior Researcher in the Center for Immunobiologicals Research and Evaluation
Involved inthe control and the evaluation of biological and biotechnological drugs by document
assessment.
Member of the Italian Advisory Committee for the Evaluation of Phase I Clinical Trials according to
DPR 439/2001,D.lvo 211/2003 and D.lvo 200/2007.
Teaching at the course “Qualità e sicurezza dei farmaci biologici e dei farmaci prodotti con
biotecnologie (biotecnologici)” (Farmaci ottenuti con biotecnologie: aspetti generali e specifici)-Società
Italiana Di Tossicologia-Roma, 2011, May 17th.
Teaching at the course “ Processi neoplastici, un problema vecchio come il mondo affrontato con le
tecnologie del XX secolo” 2013, Dicember 5th
January 2006–May 2008
Senior investigator
Istituto Superiore di Sanità, (Italy)
Senior Investigator in charge for communication projects.
Senior Researcher in the Department ofCellular Biology, National Center for Rare Diseases.
Involved with social medical research working on:“Information Service”toprovide scientific, medical,
and social information about rare diseases;“Narrative Medicine” as approach to gather relevant on
problems and needs of rare patients and their families.
Responsible of the project: “Effective communication and counselling: Improving listening in rare
diseases” (Rare Diseases, NIH-ISS).
Teaching at the course: “Narrative Medicine and patient relationship”, (Monza San Gerardo Hospital,
October 13th2006 - June15th2007.
March 2003–December 2005
Senior investigator
Istituto Superiore di Sanità, (Italy)
Senior Investigator in charge of biological and molecular procedures.
Senior Researcher in the Department of Epidemiology and Biostatistics.
Studies on genetics of health aging. These investigations had been performed using twins as working
model.
Teaching at the ISS course: “Basic Genetic Epidemiology” (held at Istituto Superiore di Sanità
October11th -13th2004).
July 1994–February 2003
Senior investigator
Istituto Superiore di Sanità, (Italy)
Senior Investigator in charge of biological and molecular procedures.
Senior Researcher in the Laboratory of Virology.
Research projects directed to investigatethe role of RAS oncogene in cell membrane communication
and HIV regulation in animal models and infected subjects. In charge to setup a microarray facility.
Collaborating with the Professor Paola Verani to the organization of an ISS internal course on
Microarray Tecnology (held at Istituto Superiore di Sanità , May 6th - 8th 2002.
Supervising the experimental thesis: “CD4 regulation mechanisms in human cell lines after HIV
infection” from 1994 to 1995.
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Curriculum Vitae
January 1989–June 1994
Simonetta Pulciani
Investigator
Istituto Superiore di Sanità, (Italy)
Investigator in the Laboratory of Virology.
Involved in Research projects directed to investigatethe role of RAS oncogene in cell membrane
communication and HIV regulation in animal models and infected subjects.
January 1988–December 1988
Investigator
Istituto Superiore di Sanità, (Italy)
Investigator in charge of biological / molecular procedures
Resercher associate at Division of Medical Genetics, Section of Molecular Genetics Children's
Hospital of Los Angeles in absence of leave from Istituto Superiore di Sanità (Rome)
Cloning oncogenes from animal tumors and characterization of proto-oncogenes functions by
transgenic mice models.
June 1985–December 1987
Investigator
Istituto Superiore di Sanità, (Italy)
Researcher in the Laboratory of Virology.
Investigator in charge of biological / molecular procedures.
Cloning oncogenes from animal tumors ( spontaneous and chemically induced).
Lesson –Seminar: “ Role of the oncogenes in experimental animal models: induced mammarymice
tumors”-Genova University- IST- Medical School for specialisation in oncology 6 April 1987.
Collaborating with Professor Giovan Battista Rossi to several experimental andtheorical PhD theses,
among those: “Animal tumors as experimental models to study oncogenes in neoplasiaonset and
development”.
December 1983–June 1985
Guest Investigator
Istituto Superiore di Sanità, (Italy)
Guest Researcher in the Laboratory of Virology, Prof. Giovanni Battista Rossi, Chief.
Investigator in charge of biological / molecular procedures.
Cloning oncogenes from animal tumors (spontaneous and chemically induced).
June 1983–December 1983
Guest Investigator
National Institutes of Health, (United States)
Investigator in charge of biological / molecular procedures.
Guest Researcher in the Laboratory of Cellular and Molecular Biology,National Cancer Institute, Dr.
Stuart Aaronson Chief, under the sponsorship of Dr. Mariano Barbacid.
Cloning oncogenes from human tumors and experimental animal tumors.
March 1980–June 1983
Visiting Fellow
National Institutes of Health, (United States)
Investigator in charge of biological / molecular procedures
Visiting Fellow of International Fogarty Center at National Institutes of Health, Laboratory of Cellular
and Molecular Biology,National Cancer Institute, Dr. Stuart Aaronson Chief, under the sponsorship of
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Curriculum Vitae
Simonetta Pulciani
Dr. Mariano Barbacid.
Cloning oncogenes from human tumors and experimental animal tumors. The major part of the work
concerned identification and characterisation of Ras oncogene in cell cultures and solid tumours.In
vitro and in vivo models to determine the molecular mechanisms involved with Ras oncogenes
activation were also developed.
June 1977–March 1980
Guest Investigator
Istituto Superiore di Sanità, (Italy)
Investigator in charge of biological / biochemical procedures.
Guest researcher at Istituto Superiore di Sanità, Laboratory of Virology, working in the research group
of Prof. G.B. Rossi.
Studies directed to analyse the Dimethylsulfoxide (DMSO)-induced erythroid differentiation of murine
Friend Leukemia Cells (FLC). The role of murine retrovirus in tumor onset and progression was also
investigated.
Teaching exercitation classes on Genetic for Biological Sciences students at Rome University from
1976 to 1979.
October 1976–May 1977
Guest Investigator
Centro Acidi Nucleici, (Italy)
Guest researcher in charge of biological / biochemical procedures.
Involved in studies to analyzethe Dimethylsulfoxide (DMSO)-induced erythroid differentiation of murine
Friend Leukemia Cells (FLC).The role of murine retrovirus in tumor onset and progression was also
investigated.
EDUCATION AND TRAINING
October 1971–October 1976
Degree in Physics
Sapienza University, (Italy)
University of Rome, Dept. of Natural Sciences, Mathematic and Physics, and Centro Acidi Nucleici,
C.N.R. -Via Romagnosi, 18/a, Rome.
Student in Physics at Sapienza-University- Rome, and Graduating student at Centro Acidi Nucleici,
C.N.R. -Via Romagnosi, 18/a, Rome, working under the sponsorship of Prof. Enrico Calef. Involved in
the of Mu Prophage replication by biological / biochemical procedures
ADDITIONAL INFORMATION
Expertise
Publications
Rossi G.B., Cioè L., Pulciani S., Meo P., Titti F., Amici D. and Gianfranceschi G.L. Effects of a
chromatin low molecular weight peptidic fraction on differentiation markers and virus production in
Friend leukemia cells. Molecular Biology Reports 5: 241-249, 1979.
Pulciani S., Santos E., Lauver A.V., Long L.K., Robbins K.C. and Barbacid M.. Oncogenes in human
tumor cell lines: molecular cloning of a transforming gene from human bladder carcinoma cells. Proc.
Natl. Acad. Sci. USA 79: 2845-2849, 1982.
Santos E., Tronick S.R., Aaronson S.A., Pulciani S. and Barbacid M. T24 human bladder carcinoma
oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV
transforming genes. Nature 298: 343-347, 1982.
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Curriculum Vitae
Simonetta Pulciani
Pulciani S., Santos E., Lauver A.V., Long L.K., Aaronson S.A. and Barbacid M. Oncogenes in solid
human tumors. Nature 300: 539-542, 1982.
Santos E., Reddy E.P., Pulciani S., Feldmann R.J. and Barbacid M. Spontaneous activation of a
human proto-oncogene. Proc. Natl. Acad. Sci. USA 80: 4679-4683, 1983.
Santos E., Pulciani S. and Barbacid M.. Characterization of a human transforming gene isolated from
T24 bladder carcinoma cells. Fed. Proc. 43: 2280-2286, 1984.
Sukumar S., Pulciani S., Doniger J., Di Paolo J.A., Evans C.H., Zbar B. and Barbacid M.. A
transforming ras gene in tumorigenic guinea pig cell lines initiated by diverse chemical carcinogens.
Science 223: 1197-1199, 1984.
Pulciani S., Santos E., Long L.K., Sorrentino V. and Barbacid M.. Ras gene amplification and
malignant transformation. Molecular and Cellular Biology 5: 2836-2841, 1985.
Pulciani S., Sakano T., Ohnishi K., Anastasi A.M., Pecorelli A., Fiorucci G., Oppi C., Rossi G.B. and
Bonavida B.. Detection of a transforming gene in spontaneous reticulum cell sarcoma of SJL/J mice:
genetically linked and host-dependent neoplasia. Cancer Research 47: 523-526, 1987. 47a)Letter to
Editor (riguardante la pubblicazione 47.). Correspondance re: Pulciani S. et al., Detection of a
transforming gene in spontaneous reticulum cell sarcoma of SJL/J mice: genetically linked and hostdependent neoplasia. Cancer Research 47: 523-526, 1987.
Ferrantini M., Pulciani S., Proietti E., Lespinats G., Anastasi A.M., Ciolli V., Rizza P. and Belardelli F..
Studies on the expression of H-2 antigens in non metastatic and highly metastatic Friend
erythroleukemia cells: correlation with the in vivo behaviour of tumor cells. Clin. Expl. Metastasis, 7:
609-625, 1989.
Sala A., Benedetto A., Elia G., Pulciani S., Ciotta C., Parlanti E., Santodonato L. and Belardelli F..
Growth properties, differentiation capacity and oncogene expression in metastatic and non metastatic
Friend Leukemia Cell variants. Inv. Metast. 11: 192-202, 1991.
Podo F., Ferretti A.,. Knijn A., Zhang PJ., Barletta B., Ramoni C., Pini C., Baccarini S., Pulciani S..
Detection of phosphatidylcholine- B. specific Phospholipase C in NIH-3T3 fibroblasts and their H-ras
transformants: NMR and Immunochemical studies. Anticancer Res. (1996) 16: 1399-1412.
Knijn A., Ferretti A., Zhang PJ,. Gianbenedetti M, Molinari A., Meschini S., Pulciani S. and Podo F..
Detection and quantification of 1H MRS-Visible Mobile Lipid Signals in NIH-3T3 fibroblasts and their
H-Ras transformantes. Cellular and Molecular Biology (1997) 43: 691-701
Rozera C., Baccarini S., Gentile M., Torrisi M.R., Proietti E., Federico M., Pulciani S.. Stable
transfection of provirus of Human immunodeficiency Virus into a murine packaging cell line. Acta
Virologica (1997) 41: 65-70.
Salvi R,. Garbuglia A.R., Di Caro A., Pulciani S., Montella F. and Benedetto A.. Grossly Defective nef
Gene Sequences in a Human Immunodeficiency Virus Type 1-Seropositive Long-Term
Nonprogressor. Journal of Virology (1998) 72: 36646-3657.
Ferretti A., Knijn A., Iorio E., Pulciani S., Knijn A., Gianbenedetti M., Molinari A., Meschini S., Stringaro
A., Calabrini A., I. Freitas, R. Strom, G. Arancia and F. Podo Biophysical and structural
characterization of 1-H-NMR-dectable mobile lipid domains in NIH-3T3 fibroblasts. Biochimica et
biophysica acta (1999) 1438: 329-348.
Fagnani C., Brescianini S., Cotichini R. et al. The Italian Twin Register: new cohorts and tools, current
projects and future perspectives of a developing resource. Twin Res Hum Genet 2006; 9:799-805.
Kodra Y, Patriarca V, Pulciani S., Trama A, Taruscio D, ed. International conference of the Network of
Public Health Institutions on Rare Diseases (NEPHIRD). Istituto Superiore di Sanità. Rome,
September 20-23, 2006. Proceedings. Rapporti ISTISAN. 2008;08(11).
Di Lonardo A, Fagnani C, Pulciani S., ed. I microarray. Caleidoscopio. 2008;25(221).
Di Lonardo A, Fagnani C, Pulciani S., ed. Applicazioni dei microarray (1). Caleidoscopio.
2009;26(222).
Di Lonardo A, Fagnani C, Pulciani S., ed. Applicazioni dei microarray (2). Caleidoscopio.
2009;26(223).
Di Lonardo A, Pulciani S. Centro nazionale per la ricerca e la valutazione dei prodotti immunobiologici
(CRiVIb). Notiziario dell'Istituto Superiore di Sanità. 2009;22(11):11-16.
Fagnani C, Pulciani S, Di Lonardo A. I microarray come tecnologia emergente per lo studio dei
gemelli. Caleidoscopio. 2009;27(223):41-49.
Di Lonardo A, Pulciani S, Fagnani C. Dalla doppia elica ai microarray. Caleidoscopio. 2008;25(221):720.
Di Lonardo A , Nasi S , Pulciani S. Cancer: We Should Not Forget The Past (Review). Journal of
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Curriculum Vitae
Simonetta Pulciani
Cancer 2015; 6(1): 29-39. doi: 10.7150/jca.10336
Projects
Memberships
Other Relevant Information
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