Title Author(s) Citation Issue Date SPORE-SIZE VARIABILITY IN SUBSEQUENT SPORE PRINTS OF SOME HYMENOMYCETOUS FUNGI Togashi, Kogo; Oda, Kojiro 札幌博物学会会報 = Transactions of the Sapporo Natural History Society, 13(3): 121-125 1934-06-20 DOI Doc URL http://hdl.handle.net/2115/64122 Right Type article Additional Information File Information Vol.13No.3_006.pdf Instructions for use Hokkaido University Collection of Scholarly and Academic Papers : HUSCAP SPORESIZEVARIABILITYINSUBSEQUENTSPORE PRINTSOFSOMEHYMENOMYCETOUS FUNGI 国 BY KOGO TOGASHI AND KOJIRO ODA 〈富樫治吾・ 4、 岡 耕 次 郎 〉 Spores i z ec o n s t i t u t e s ani m p o r t a n tc r i t e r i o nf o rd i s t i n g u i s h i n gt h es p e c i e s ofa l m o s ta l lf u n g i ,andi nt u r nt h en o n c o i n c i d e n c eo fs p o r e measurementsmay o f t e nl e a dt h ei n v e s t i g a t o r st od i v e r g e n to p i n i o n sa st oc l a s s i f i c a t i o n . As t o t h ev a r i a b i l i t y owingt oe n v i r o n m e n t ,m a t u r i t y ,e t c . ,t h e r ea r e many d a t aa c cumulatingi nm y c o l o g i c a l andp h y t o p a t h o l o g i c a ll i t e r a t u r e . B u t ,a sf a ra st h e w r i t e r sc o u l da s c e r t a i n ,no r e p o r t swhich d e m o n s t r a t es p o r ev a r i a b i l i t yo f hymenomycetousf u n g icaused by t h er e p e t i t i o no fs p o r ep r i n t i n gw i t ht h e same p i l e u s have been p u b l i s h e de x c e p tt h a to f ZELLERand t h es e n i o rw r i t e ro f R ,S . M. and TOGASHI, K.: The American and J a p a n e s e t h i spaper (ZE比 E M a t s u t a k e s . Nowi np r e s s ) . A f t e rh a v i n gmadeb i o m e t r i cs t u d i e stheyp o i n t e d r m i l l a r i a Matsutake !Toe tI M A I become somewhat out t h a tt h es p o r e so fA s m a l l e randrounderi nt h el a t e rs p o r ep r i n t s ,anda l s ot h a tt h er a n g eo fv a r i a b i l i t yo fs p o r es i z e sbecomesn a r r o w e r . I ti st h eo b j e c to ft h i sp a p e rt oi n q u i r e whethert h i stendencyi ns p o r es i z e s mayo c c u ri nt h eo t h e rc a s e so fhymeno・ mycetousf u n g io u t s i d eA. . l l f a t s u t a k e . Fort h epurpose o ft h i ss t u d yf r e s hm a t e r i a l so ft h ef o l l o w i n g hymenomycetousf u n g iwere s e l e c t e d;namelyA r m i l l a r i aw 伽 (VAHL.)FR.,めJ j J h o・ lomas u b l a t e r i m ScHAEFF., 1功o l i o FR. As u s u a li ng e t t i n gs p o r ep r i n t s , af u l l yd e v e l o p e dp i l e u sfrom whicht h e stem hadbeen removed was p l a c e dw i t ht h eg i l l s downward on a s h e e to f paper and wascovered withab e l lg l a s st o keep t h ea i rfrom blowing t h e s p o r e s away. J u s t onedayl a t e rt h ec o v e r i n gand t h ep i l e u s wereremoved. Thus, fromt h e same p i l e u sf o u rs p o r ep r i n t s , one p e rd a y , were o b t a i n e da t t h er e g u l a rt i m e s , andt h es p o r e s from t h ep r i n twere u s e df o rmeasurements nmeasuringt h es p o r e sb i o m e t beingmountedi nwaterd r o p sonag l a s ss l i d e . I r i cc o n s t a n t s were c a l c u l a t e d byt h eassumed mean method. Thef o r m u l a e n n z l l a r i aM a t s u t a k e . usedwere t h esamea si nt h ec a s eo fA Ther e s u l t so ft h emeasurementsw i l lbeshowni nt h eaccompanyingt a b l e s . a t .H i s t .S o c . ,V o l .X I I I ,P t・3 ,1934J [ T r a n s .SapporoN TRANSACTIONS OFTHESAPPORO NATURALHISTORY SoCJETY 122 i o m e t r i cd a t aont h el e n g t h and widthofeach mos p o r e s Table I. B fromt h e same p i l e u so fA n n i l l a r i am e . ルa Length i n microns ~~~~ i 5 I s l 6 7 8 9 II Mean Standard d e v i a t i o n Coe 伍c i e n tof v a r i a b i l i t y ICO . 0 6 7 . 6 0土 0 0・ 92士0 . 0 4 . 5 8 I Z . 2 0土 0 . 0 5 6 . 7 0土 0 0 . 7 6士0 . 0 3 IE・ 46士0・ 54 1 3 JOO 6 . 7 1士 口. 0 4 o . 6 8土 0 . 0 3 9 . 8 7土 0・ 47 1 3 100 . 0 4 6 . 6 3士0 0 . 7 0土 0 . 0 3 5 ' l 1 0 . 5 9士0・ 27 35 46 18 3rd 42 45 4th 50 37 I Total 100 1 4 46 nd 宮 IO 宮 Width i nmicrons Spore p r r n t 3 I s t I 2nd 3rd Mean 日t andard d e v i a t i o n Coe 侃c i e n to f v a r i a b i l i t y 5 6 39 59 I 100 . 0 3 4 . 6 0土 0 0・ 52土 0 . 0 2 . 5 3 I l . 3 3土 0 89 II 100 . 口2 4 . 1z土 o 0 . 3 1士O . O I 36 7 . 6 1土 0・ 3 2 IOO . 0 1 4 . 0 3土 0 . 0 1 0 . 1 7土 0 4 . 2 3土 0 . 2 0 100 4 口 ・2士0 . 0 1 . 0 1 0 . 1 4土 0 3・ 48土 0 . 1 6 97 98 4th T o t a l 4 Table 2. B i o m e t r i cd a t aont h el e n g t hand width ofeach100s p o r e s from t h e samep i l e u so fP h o l i o t aa d . タ・ o s a Length i n microns Spore prmt 6 7 8 9 IO 4 I T o t a l Mean Standard d e v i a t i o n C o e f f i c i e n tof v a r i a b i l i t y I O・ 5宜土0・ 50 1 s t 1 2 33 50 100 . 0 5 7 . 4 8土 0 0・ 78土 0 . 0 4 2nd 54 43 3 100 6 . 4 9土 0 . 0 4 . 0 3 055土 0 8 . 5 7土 0・ 41 3rd 55 42 IOO 6 . 4 8土 0 . 0 4 0・ 5 5土 0 . 0 3 8 . 5 8土 0 . 4 1 4th 64 3 34 2 100 . 0 4 6 . 3 8土 0 0 . 5 2土 0 . 0 3 8 .宮Z土 0 . 3 9 nmicrons Width i prmt 4 5 6 T o t a l Mean 1 s t 44 100 53 3 2nd 100 59土0・ 04 4・ 3rd 100 100 4th 100 100 JOO Standard d e v i a t i o n 0 . 5 4土 0 . 0 3 Coe 伍c i e n tof v a r i a b i l i t y 日 ・97土 0 . 5 7 TOGASHI& ODA: SPORE-SIZEVARIABILITYI NSUBSEQUENTSPOREPRINTS 123 Table 3 . B i o m e t r i cd a t aont h el e n g t handw i d t ho fe a c h100s p o r e s fromt h esamep i l e u so f再汐'holomas u b l a t e r i t z " u m Length i nm i c r o n s ~~~ 3 4 5 6 1 s t 3 64 20 100 2 1 100 1 3 100 8 JOO 2nd 4 1 3 18 3rd 4 19 57 64 5 章 。 67 4th T o t a l Standard d e v i a t i o n 白e 伍c i e n to f 5 . 0 1土0 . 0 4 0 . 6 7土0 . 0 3 3 8土0 . 6 3 1 3・ . 0 4 4 . 9 5土0 ・ 94土0 . 7 1 1 4 4.86±0.04 0・ 73土0・ 03 o . 6 8土0 . 0 3 78土0 . 0 4 4・ . 0 3 0 . 6 5土0 74土0 . 6 5 1 3・ Mean v a r i a b i l i t y 1 4 . l l土0 . 6 7 Width i nm i c r o n s ~~~~ 3 4 宮9 宮E JOO JOO JOO JOO Standard d e v i a t i o n 伍c i e n to f O>e v a r i a b i l i t y 3 . 2 9土0 . 0 3 0 . 4 5土0 . 0 2 . 0 3 3 . 2 1土0 0・ 40土0 . 0 2 13・ 79土0 . 6 5 . 6 0 1 2 . 6 8土0 3 . 1 6土o . 白書 0・ 3 7土ロ. o宮 J I . 6 0土0・ 55 3・ 1 4土0 . 0 2 35土0 . 0 2 0・ ロ. 0 2土0・ 52 Mean 1 s t 71 2nd 79 3rd 8 4 1 6 4th 86 14 Table4 . B i o m e t r i cd a t aont h el e n g t handw i d t ho fe a c h100s p o r e s fromt h esamep i l e u so fG砂b i a切 l u t z ' p e s Lengthi nmicrons ~~: I 1 s t 宮nd 3rd ・ 4th 一 7 8 54 28 54 23 14 63 21 3 I 5 6 57 量7 Me 唱n Standard d e v i a t i o n 伍c i e n to f Coe v a r i a b i l i t y 100 6 . 1 9土0 . 0 5 0 . 7 1土0 . 0 3 u.58土0・ 55 6 . 0 3士0 . 0 5 0 . 7 0土0・ 03 u.59土0・ 55 量 J O 。 ' JOO 6 . JE士0 . 0 4 0 . 6 4土ロ. 0 3 1 0 ・ 58土0・ 50 7 100 6 . 3 宮土 0 . 0 5 0・ 73土0 . 0 3 55 J I . 6 0土0・ 1 5 宮Z 9 T o t a l Widthi nm i c r o n s Standard d e v i a t i o n Coe 侃c i e n to f v a r i a b i l i t y ~~~; 2 3 T o t a l Mean I s t 70 30 宮E JOO ・ 宮30土0 . 0 3 045土0 . 0 2 1 9 ・ gz土0 . 9 5 100 2 . 宮Z土0 . 0 3 . 1 9 0 . 4 0士0 . 8 8 1 8 . 4 2土o 宮5 100 . 0 3 2 . z 5土0 0 . 4 3土o . 白書 1 9 虫 ・4土0・ 92 1 7 lCO 宮. 1 7土0 . 0 3 .o ・ 37土0 . 0 3 ・ 3 1土0 . 8 3 1 7 宮nd 3rd 4th 79 75 83 T R A N S A C T I O N SO FT H ES A P P O R ON A T U R A LH I S T O R YS O C l l I T Y 124 i o m e t r i cd a t aont h el e n g t handwidtho feachJOOs p o r e s T a b l e . 5・ B o l ! y b i av e l u t . がe s( c u l t u r e d ) fromt h esamep i l e u so fC L e n g t hi nm i c r o n s p r i n t 5 6 7 8 1 s t 2 n d 3 r d 4 t h 7 1 6 1 6 7 5 9 4 9 5 1 5 5 2 5 2 9 2 8 3 0 6 5 8 9 1 0 0 E 口 口 M e a n S t a n d a r d d e v i a t i o n Coe 伍c i e n to f 6 . 3 6土口. 0 5 6 . 2 5土0 . 0 5 6 . 2 2土0 0 5 6 . 3 9土0 . 0 5 0 . 7 4土0 . 0 4 0 . 7 9土0 . 0 4 ・ 7 6土0 . 0 4 0 0 . 7 3土0 . 0 3 I l . 6 6土0 ・ 5 6 1 2 . 6 7土0 . 6 0 1 2・ 3 6土0 . 5 0 ) I E ・ 4 7土0 ・ 5 ヨ M e a n S t a n d a r d d e v i a t i o n Coe 伍c i e n to f 1 8士0 . 0 3 2 . 2 8土0 . 0 3 3 2 . 1 8土白田0 宮. 1 5土0 . 0 2 ・ 3 8土0 . 0 2 0 0 ・ 4 4土0 . 0 2 0 ・ 3 8士0 ・ 0 2 . 0 2 0 . 3 5土0 1 7 . 6 2土0 . 8 4 1 9 . 6 9土0 . 9 4 . 8 . J 1 7 . 6 2土o 1 6 . 6 0士0 . 7 9 v a r i a b i l i t y ¥ V i d t hi nm i c r o n s p r i n t ぉt I 2 n d 3 r d 4 t h 宮 8 2 7 2 8 2 8 5 3 1 8 2 8 1 8 1 5 I O コ 1 0 0 1 0 0 JOO 宮 v a r i a b i l i t y Asa l r e a d ymentionedi nt h ec a s eo fA r m i l l a r i aMatsutaket h es p o r edimens i o n s ,e s p e c i a l l yt h el e n g t h ,o ft h ef o u r ・f u n g i usedbecome s m a l l e r and cons e q u e n t l yt h es p o r e s become rounder i n shape w i t ht h er e p e t i t i o n s of s p o r e p r i n t i n gw i t ht h esamep i l e u s . I nC o l l y δi av e l u 砂町 however,t h i s tendencywas n o ts oc o n s p i c u o u s and even i n somei n s t a n c e st h es p o r e s were s l i g h t l yg r e a t e r i nl e n g t ho ri n width ( T a b l e s 4 and 5 ) . Themosts t r i k i n gd e c r e a s ei n spo 肥 d i m e n s i o n s was observed i nP h o l i ・ o t aa d i p o s a showing an extreme d i f f e r e n c e betweent h e means c a l c u l a t e d on t h es p o r e sfromt h ef i r s tandf o u r t hp r i n t sof I .IOμ i nl e n g t h and0・58f li nwidth ( T a b l e 2) . I nt h ec a s e so fArm. グ' Z a r i a y p h o ! o m as u b ! a t e r i t i u mt h eextremed i 民r e n c e sofl e n g t handwidth m e l ! e aand正{ were0・ 97μand0・58μ (Table1 ) , and 0 . 2 3f 1and0 . 1 5f 1( Table3 ) ,r e s p e c t i v e l y . I ti so fi n t e r e s tanda l s onoteworthyt h a ti nt h etwoc a s e s ,offう~oliota a d i p o s a and A r m z l ! a r i am e ! ! e a ,t h es p o r e s from t h e second p r i n t showed an abrupt d e c r e a s ei nd i m e n s i o n scompared w i t ht h o s efromt h ef i r s tp r i n tanda f t e rt h a t ag r a d u a ld e c r e a s etookp l a c e . One morei n t e r e s t i n gf a c ti st h a ti nt h e subs e q u e n ts p o r ep r i n t so ft h ef o u rf u n g it h ev a r i a b i l i t yofs p o r ed i m e n s i o n s ,both i nl e n g t hand w i d t h , became c o n s i d e r a b l y narrower and t h e mode of spore c l a s s e s removed t ot h elower s i d e ,w i t ht h eonee x c e p t i o no fG砂 b i av e l u t i p e s . I nc o n c l u s i o nt h ew r i t e r s cani n f e rw i t hc e r t a i n t yt h a ti n hymen::>mycetous f u n g i ,a tl e a s ti nt h o s et h a tproducee l l i p s o i d a lo rs i m i l a r shaped s p o r e s , the T O G A S H I& ODA: S P O R E S I Z EV A R I A B I L I T YI NS U B S E Q U E N TS P O R EP R I N T S 125 s p o r e sa r ea p tt o showsomewhat s m a l l e randrounderi ns i z e and shapewhen they a r e examinedone o rmore days a f t e rt h ef u n g ia r ec o l l e c t e d ,s i n c et h e f u n g imayd i s c h a g et h es p o r e sc o n t i n u o u s l yandf r e e l ya sl o n ga st h ep i l e i do n o t dryo u to rr o t . I n v e s t i g a t o r ss h o u l db e a ri nmind t h e s ef a c t si f th~y t r y か afungus ofthis group. t oi d e n t i B o t a n i c a lL a b o r a t o r y ,M o r i o k aC o l l e g eo f A g r i c u l t u r ea n dF o r e s t r y ,M o r i o k a ,J a p a n . STUDIESONTHEDOWNYMILDEWS OFCRUCIFEROUSVEGETABLESINJAPANI BY MAKOTOHIURAANDHIDEOKANEGAE (樋浦誠・鐙江英夫) Manyk i n d so fc r u c i f e r o u sv e g e t a b l e su s e f u lt ot h ed a i l yl i f eo ft h eJ a p a n e s e a r e widelyc u l t i v a t e dthroughoutt h et e r r i t o r yo fJ a p a n . Most o fthem belong t ot h egenusB r a s s i c ao rRa1 幼a n u s , and a r ecommonly s u b j e c tt ot h ea t t a c k ofa丹 仰 仰 乎o r afungusknownf o r m e r l ya s 丹 仰 仰1 s p o r aparas がc a( P E R S . )F R . , e r o n o 学o r ab r a s s i c a e GM. butl a t e l yo f t e nd e s i g n a t e da sP h a t I t seemst obei n f e r a b l e fromt h er e c e n ti n v e s t i g a t i o no fG孟UMANN(S)t t h efungusp a r a s i t i cont h e s ec r u c i f e r o u sv e g e t a b l e smightc o m p r i s ev a r i o u ss t r a i n s e n ti np a t h o g e n i c i t y . Toa s c e r t a i nt h i sa s s u m p t i o n , somei n o c u l a t i o ne x p e r i d i能 r ments have beenc a r r i e do u t , and t h er e s u l t ss of a ro b t a i n e da r et e n t a t i v e l y p r e s e n t e di nt h i sp a p e r . Materials andMethods A f f e c t e dl e a v e so fv a r i o u sc r u c i f e r o u sv e g e t a b l e sfoundi nt h eneighbourhood o fG i f uA g r i c u l t u r a lC o l l e g e were c o l l e c t e d ,a s ar u l e , towardse v e n i n g ,washed i nrunningw a t e r ,andeachkepti nam o i s ts a t c h e li nt h el a b o r a t o r y . I nt h i sway, anabundance o ff r e s hc o n i d i aweree a s i l ya v a i l a b l e on t h ef o l l o w i n g morning. The s e e d s usedi nt h e s e experiments were a l lg o t t e na ta s e e ds t o r ei nG i f u . Theyweresownand grown i no r d i n a r yp o r c e l a i np o t s . [ T r a n s .S a p p o r oN a t .H i s t .S o c . ,V o l .XIII,P t .3 ,1 9 3 4 1
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