ヒト血清からのアルブミン除去

ヒト血清からのアルブミン除去
A 280
20.0
Buffer Buffer
A
B
Buffer
A
Column:
Sample:
HiTrap Blue HP 5 ml
3 ml human serum,
buffer exchanged on a
PD-10 column to buffer A
Buffer A:
50 mM KH2PO4, pH 7.0
B:
50 mM KH2PO4,
1.5 M KCl, pH 7.0
Chromatographic 5 ml buffer A, 3 ml
procedure:
sample, 20 ml buffer A,
10 ml buffer B,
10 ml buffer A
Flow rate:
1 ml/min
10.0
0
5
pool I
10 15
20
25
30
pool II
35 40
45 ml