ヒト血清からのアルブミン除去 A 280 20.0 Buffer Buffer A B Buffer A Column: Sample: HiTrap Blue HP 5 ml 3 ml human serum, buffer exchanged on a PD-10 column to buffer A Buffer A: 50 mM KH2PO4, pH 7.0 B: 50 mM KH2PO4, 1.5 M KCl, pH 7.0 Chromatographic 5 ml buffer A, 3 ml procedure: sample, 20 ml buffer A, 10 ml buffer B, 10 ml buffer A Flow rate: 1 ml/min 10.0 0 5 pool I 10 15 20 25 30 pool II 35 40 45 ml
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