Compact stand-alone FPLC as a quick and reliable solution for protein purification in laboratory scale Highest reproducibility thanks to pre-programmed chromatography parameter Suitable for a multitude of chromatography applications (AC, AIEX, CIEX, DS und GF) ÄKTA™ start is a compact, affordable protein purification system (FPLC) repre sents a quick and reliable solution that removes the hassles of manual approaches and provides automated control of the purification procedure. ÄKTA™ start is designed as a standalone system that combines a front-facing flow path with a user-friendly interface. The system is controlled via an intuitive touch screen, which displays the UV curve and other run data in real-time. ÄKTA™ start allows the implementation of all common purification techniques, like affinity, ion-exchange, desalting and buffer exchange, as well as gel filtration. The system therefore supports purification of tagged proteins, antibodies, untagged or native proteins and sample clean-up. ÄKTA™ start can be run using predefined quick-start protocols, the built-in templates or by creating custom protocols. Application-focused prepacked columns are offered for common applications. ÄKTA™ start can be complemented with the Frac30 fraction collector for automa- tic protein collection within volumes of 0.5 ml and 15 ml, and the user-friendly UNICORN™ start control software, which provides additional evaluation tools and the possibility of controlling ÄKTA™ start from a computer. APPLICATIONS - Affinity chromatography (AC) of proteins, antibodies, untagged or native proteins and sample clean-up -Anion-exchange chromatography (AIEX) - Cation-exchange chromatography (CIEX) - Protein desalting (DS) -Buffer exchange / Gel filtration (GF) PRODUCT HIGHLIGHTS - Automated protein chromatography system a quick and reliable solution for protein purification in laboratory scale -Easy to use because of predefined quick-start protocols or the user-friendly UNICORN™ start 1.0 PC control software - Real time run and monitoring of UV and conductivity measurements -Highest reproducibility because of pre-programmed chromatography parameter (flow rate, gradient formation and pressure) - Large selection of optimized prepacked columns for all common purification techniques - Fraction collector for automatic protein collection of up to 30 fractions in four different tube sizes (1.5, 5, 10 and 15 ml) SPECIFICATIONS -System control with integrated Touchscreen display or UNICORN™ start 1.0 Software - Flow rate 0.5 – 5 ml/min - Gradient flow rate 0.5 – 5 ml/min - Maintenance free self-priming pump 0 – 5 bar (0 – 0.5 MPa) - Direct UV monitoring technology (280 nm) - Conductivity monitor 0 – 300 mS/cm with integrated temperature sensor - Buffer inlets: 2x Buffer ports 1x sample - Fraction collector (FRAC30): 30 fractions à 0.5 – 15 ml - Dimensions (W x H x D): 340 x 360 x 280 mm - Weight: 8 kg PRODUCT DESCRIPTION CAT. NO. ÄKTA™ start System compact and automated chromatography system (FPLC) 29-0220-94 Fraction collector FRAC30 automated fraction collector for 30 fractions (0.5 – 15 ml) 29-0230-51 UNICORN™ start 1.0 Software ÄKTA™ start control software 29-0187-51 Fon +49 9131 610 70 - 20 · Fax +49 9131 610 70 - 99 Freecall(D) 0800 100 2016 · Freefax(D) 0800 100 9016 ORDERING INFORMATION • ÄKTA™ start CHROMATOGRAPHY SYSTEM ÄKTA™ start chromatography system High quality pre-packed columns compatible with a wide range of reducing agents and detergents High binding capacities Ideal for ÄKTA™-systems ÄKTA™ start designed as quick and reliable solution that removes the hassles of manual approaches uses Quick start 'ready to run' methods to COLUMN HisTrap™ HP Column HiTrap™ TALON ® Crude Column HiTrap™ Desalting Column GSTrap™ 4B Column purify most common proteins based on affinity, ion-exchange, gel filtration and desalting techniques. Application-focused pre-packed columns are offered for common applications. MATRIX BINDING CAPACITY (Proteine/ml medium) APPLICATIONS Quick start METHOD Ni Sepharose 40 mg Histidine-tagged Proteins (Ni2+ -AC) AC step HiTrap TALON ® 20 mg Histidine-tagged Proteins (Co2+ -AC) AC step HiTrap Sephadex G-25 Superfine – Protein desalting Buffer exchange DS HiTrap Gluthatione Sepharose 4B 25 mg GST-tagged Proteins (AC) AC step HiTrap HiTrap™ Protein A HP Column Protein A Sepharose 20 mg (IgA) Antibody exchange AC step HiTrap HiTrap™ Protein G HP Column Protein G Sepharose 25 mg (IgG) Antibody exchange AC step HiTrap HiTrap™ Q HP Column Q Sepharose – Anion-exchange chromatography IEX step HiTrap IEX gradient HiTrap HiTrap™ SP HP Column SP Sepharose – Cation-exchange chromatography IEX step HiTrap IEX gradient HiTrap Sephacryl S-200 – Gel filtration GF 16/60 HiPrep™ Sephacryl S-200 Column PRODUCT DESCRIPTION CAT. NO. HisTrap™ HP Column Ni - Sepharose High Performance Column (1 ml) 29-0510-21 TALON ® 29-0485-65 HiTrap™ TALON ® Crude Column Superflow Column (1 ml) HiTrap™ Desalting Column Sephadex G-25 Superfine Column (5 ml) 29-0486-84 GSTrap™ 4B Column Glutathione Sepharose 4B Column (1 ml) 29-0486-09 HiTrap™ Protein A HP Column Protein A Sepharose High Performance Column (1 ml) 29-0485-76 HiTrap™ Protein G HP Column Protein G Sepharose High Performance Column (1 ml) 29-0485-81 HiTrap™ Q HP Column Q Sepharose High Performance Column (1 ml) 29-0513-25 HiTrap™ SP HP Column SP Sepharose High Performance Column (1 ml) 29-0513-24 HiPrep™ Sephacryl S-200 Column Sephacryl S-200 Column (5 ml) 17-1166-01 Fon +49 9131 610 70 - 20 · Fax +49 9131 610 70 - 99 Freecall(D) 0800 100 2016 · Freefax(D) 0800 100 9016 ORDERING INFORMATION • ÄKTA™ start PRE-PACKED COLUMNS ÄKTA™ start Pre-packed columns
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