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1.
ƃƥ
Ǔ¬ŔĖ$őwǗÃ%Ő¹ƧƌÐgőŒ£ƈ"ǗÃŻē5őŒ³$²#ǈ5£ƈé0Ñ
"ØõƀŚ"œ£ƈ"ǗÃƨĽ4ŠįŔĖ$Zb[h$sĎƨĽ 0ĈƗ$œưǁř%
ƻƕ5őwǗÃǀ$¾%ġƱ¾Ŷǒ569Õğć$ƘŅ3Ɩŝr{$ǗĤ
ƈéŻē Ĳœ6&őňƨĽ$ĈǗÙœ#ƨ5&4"Ǔ¬Œğ$¾ƚŚ"Óǁ 0đá5
Ēšū%őwǗÃ ?Qg=WĨ"! Úĝ65ƨĽ3ä365 chitosan #
¡è
é#Ë.:\S·9$ǗÃǀ#¾Đ5 #şŜŉÈ$Ãũ#Ǘƶðé9Đ5b@fO9
Å}ǔ#2°È
JfUBƩ1YXKOů$Ǝć "5T;>ŷĤƈéÃ$ǋ9Ơ-
ŉ#
ǗƯƴĳĔ9ÉŎ5/ƒǎāƩ9Ċ #24Ɖ$ÉƩŚ"ƒǎŪ9ºƠ-9Ƒ,Ł
Ų9ć Ǚƹĩ$iũ5 Aureobasidium pullulans 9¶Ǖä365¾Ŷǒ$ pullulan
ĴƏŔĖ$Ǘ
Ã(κ-carrageenan) $Ɣ¥9Ơ-
Ēšū$ŜŚ%ÃE;HƣƦƈ$ŗ"5őwǗÃƉ9Ɣ¥5ŶǀRLNehB$Å}ǔ å
#2ǗƯƴĳĔ ÃƣƦĤƈ9řŎÃE;H#èÃƅ$ƶðǋ9¾ħŚ#ƑXd
IG9ǁř5 5$/#Chitosan Ɖ$ƤƓ#
%ľÂ$ǗĳĔ9ÉŎ5/æżų
Ã#25ƿ´Þí9ANEfƉ$ƒǎāƩ$îİ ÏƉ$ĤĜŚÜÙ9þ" "ƉiėÝ4
$ĳĔ$§j9¯4iĉÃƣƦĤƈ$b@fO JfUBƩƙ«é$Ǘ Cibacron Blue F3GA 9Ɖ$
Å}ǔ ÏJfUBƩ$ƶðŚ©ş Ã(5Ę9¥80ǗĳĔ$ǗÙǋ9Ƒ
56#2ǋĀx$jĳǚUp StreamǛ9ñÝ5ǋƉ $£ƈé9ƭĭ5,n
ũ$őwǗÃǚpullulan and κ-carrageenanǛ$Ɣ¥ ŶǀRLNehB$ġƱå$öĢ $ŵÊ"ÃE;H$ƣƦƈ#2ǋĀx$kĳ
ǚDown StreamǛ
κ-carrageenan Ä²¥ (FC) #ıŜ
9ñÝ5ǋƉ $£ƈé9ƭĭ563iƲ$šūíĘ#24Ǔ¬ŔĖ$őwǗÃ$Ɣ¥
Å}ǔ#24ŶǀRLNehB$å9ÉŎıŜí$ŉé#èǋĀx$jĳ kĳ9
66ñÝ5ǗÃƉ9ĈƗ#ƤƓǋĀx$¾ħŚƲƀŚ"XdIG9üě5
2.
Chitosan 9·ē 5ǗĳĔJfUBƩǋƉ$ǁř
2.1 Silica æųÃ#25ƒǎƿ´āƩ#25ǗĳĔ$ÉŎ
ÃƣƦĤƈƉ# ľÂ$ƯƴĳĔ$¿%ıŜí#Í5ƶðé #ƻƕ5
Ēšū%
iė$Ɖ$ÉƩŚƒǎŪ9ºÕÁ Ɖ$ĤĜŚÜÙ9þ" "æż"ġƱ9æųÃ$ƿ´
ƒǎ#ÞíƉ$ǗĳĔ$ÉŎ9¯"8chitosan-ƸƺĬľķ# silica æųÃ9ìłæ
ųÃ9Ç#Įǅâ#mŇmŇƉ# 1.5M ĬƺPNb<]ľķ9ıƺ9l«ĻņƉƒ
ǎ$ silica æųÃ9Ǉ æż"ġƱ9ĊļŀƉ9ä (Figure 1)
(a)
(b)
Figure 1. SEM image of surface modified chitosan membrane (a) imprinted surface (b) original surface
1 Figure 2 #Ɖǎ$±Ö9úƳ 5ŹĬƯƴ$žĘ24silica æųÃƒǎŏ6Ɖ%Č3#Ǘ
ŹĬƯƴĳĔ9Ť6%ƓƉč#Ï silica æųÃƉƒǎ#ƿ´ $æųÃľƪ
#Ɖæż"ġƱőÉƩŚ#ƉiėÝ4$ƒǎŪ9º Ɔ365624silica æų
Ã$ųÃà1Ïƽ9¼5 #24ƉǎŪ$ÉƩŚ"º¿903Ɖƒǎ$āƩ9å5£ƈ
é5
(a)
2
: non-modified
Pure water permeation [g]
Pure water permeation [g]
2
: surface modified
1.5
1
0.5
Chitosan : Silica (g:g)
: 1:1
: 1:0.6
: 1:0
1.5
1
0.5
0
0
0
20
40
60
0
80
20
Time [min]
40
60
80
Time [min]
Figure 2 Elevation of pure water permeability (a) Effect of surface modification, (b) Effect of added amount of silica particles
(Operational pressure: 100KPa)
2.2
Chitosan 9·ē 5ǗĳĔJfUBƩǋƉ$ǁř
JfUBƩ$ƶðb@fO Cibacron Blue F3GA $Nb:FfŐ#5Bdd· ANEf$:\S·
$ĭĚƄý¡è#2Đž¥9Þíchitosan Ɖ#JfUBƩ©şb@fO9Ï±9úƳ
5JfUBƩĬľķ$ƯƴÉǖ%Pepsin (34KDa)Egg Albumin (43KDa)$JfUBƩ#ÍǄĦō*
+ 90%#Ƶ¿"Ãƅ$sƒ JfUBƩ (BSA) b@fO#2Ɖǎ#ùŌ6 #2
Ð"ǋ9¨.ľÂǗĳĔ$0 #Ưƴkĳ#vƄ5$ǗÃƉ$ŶǀRLNehB#2
Ð"Ãƅ$ŕǋ¾ħŚ#ÉŎ65£ƈéƖ6
3.
ĈƗ κ-carrageenan/pullulan Ɣ¥Ɖ$í
ƓƉ%A`GNİ9·Ēîİ ęģ$ƶð ĸĉİ#ŉ#ŖêǚŃÙgĸ$J;\fCǛ9ï
ŶǀRLNehB$ġű9¯ď#CcJc:cMVO#25ęģ6ǗÃľķ9@aG$×Ŀǎ
j#ĳĶĹmŇ#24mŇƉ9íÞ5,Ɣ¥$iĉ$í5 κ-carrageenan $nƻƐċÃ
ġƱ%?b<];>f#24 pullulan
ęģ5 5624Ɣ¥Ɖ%Éœ#Ƈ365ĤĜŚÜÙ
9ä
Ēšū$îİ#2ļŀŊë0ÆÈ"×Ŀǎ9Đ5 κ-carrageenan/pullulan Ɣ¥ƊŮƉ$Ŏé$Ǘ
ƤƓİ9ţŮ
4.
κ-Carrageenan/pullulan Ɣ¥Ɖ#Í5ŃÙƷ9úƳ ÃE;HƣƦĤƈ$Ɵ{
4.1 Éǖĉİ
κ-carrageenan/pullulan Ɣ¥Ɖ9iÍ$@aGIc÷-Ɖq36zſŞ ƯƴŞ$ǂ#őöĢÃ
$ŃÙƷ9úƳ 5óĄƯƴ9ƑƉ$ĐóĄ|ą9ĭ/5 #2Ɖ$ÃE;HƣƦĤƈ
9Ɵ{öĢÃ%Ɖ9ġí5ǗÃ ǍǌŚŘĬŚŞoxœ#lŮéǗÃE;H$
_Mc "5#(8ňƩ9ƶƆ"8Urea (60Da)Glucose (180Da)Methyl Orange (327Da)Indigo
Carmine (466Da)Bordeaux S (604Da)Brilliant Blue (826Da)Rose Bengal (1017Da)5
2 4.2
žĘ
2'ƆÌ
¤íŋŷ$ƯƴĳĔ#·Ɖ$ĐóĄ|ą9ĭ/Figure 3 #ŤžĘ24Ãƽ 17
¼5 (60~1017Da) $¼µ#
ĐóĄ|ą 15,000 ¼5 9Ɩκ-carrageenan/pullulan
Ɣ¥ƉĠ/ƾă"Ãŕéƈ9Đ5 9$Ƽ/Č3#Ãƽ 327Da rƬĐ
óĄ|ą$¼ď0ƍ 3Ɖ$ƯƴŽƫ%ÝơÃE;H(Ÿ 11Å)#Ǌlû5 Ť6Ãƽ$¼µ#%Ǔ¬ŔĖ$Ĥƈéíǚ:\SƺgWJ\fůǛ¾Ä²
463$
Ĥƈéí$ÃE;H#·ƶðǋƉ đá5
10-8
DW & Deff [m2s-1]
10-9
A
10-10
10-12
10-13
10-15
100
B
FC0.66
C
10-11
10-14
MW [Da] MS []
DW (Wilke & Chang)
A: Urea
B: Glucose
C: Methyl Orange
D: Indigo Carmine
E: Bordeaux S
F: Brilliant Blue
G: Rose Bengal
D
MW : Ãƽ
E
MS : ÃE;H
60
180
327
466
604
826
1017
6.0
8.9
10.5
11.9
13.0
14.4
15.8
F
G
FC0.33
101
102
103
104
Molecular Weight [Da]
Figure. 3 Effect of molecular weight on the effective diffusion coefficient of κ-carrageenan/pullulan composite membrane.
5.
κ-Carrageenan/pullulan Ɣ¥Ɖ#Í5±9úƳ 5ňƩƯƴéƈ$Ɵ{
5.1 Éǖĉİ
κ-carrageenan/pullulan Ɣ¥Ɖ9ÿòÛǆ½ńƴIc#ƝƄŭŻ@G$±9ŹĬƯƴ$úƳ Ɖ9Ư
ƴĬ$Ʃƽ9ǌÃÀŦ #24ŽčŚ#ĺÈĬ$ÊÙ#24wŪ)ýŰƉǎvǎŪgvƉg
včǂÝ4$ŹĬ$ƯƴĳĔ JV [m3gm-2gm-1gs-1]Ű¨Ĭō$ĺÈ ńź9œƉƒǎ$r
şĬ9¢4ǇǌÃÀŦ#24ļŀƉ$Ʃƽ(we)9ĺÈļŀƉ9 343K(24 čǂ)mŇâmŇƉ
$Ʃƽ(wd)9ĭ/¨Ĭō%(we-wd)/we 24Ű$¨Ĭō%ļŀŊë$Ɖ$Ŭǉō Ɔ5 5
5.2 žĘ
2'ƆÌ
ƙĬéǗÃƉ# ĬÃ$Ưƴéƈ$Ɵ{%ľÂÃ$ƯƴĤġ#ǃ5·ś ƻƕ5
κ-carrageenan $Ʃƽ¥ FC $º¿#ãƉ$¨Ĭōº¿6%ǗÃ$Ţƺ·%ƙĬéǗ
/ Ɔ365κ-carrageenan/pullulan Ɣ¥Ɖ$ŹĬ$ƯƴĳĔ%Āx±#iĥĪyº¿ 3Ĭ
%ǗÃƉ$ƯƴŽƫ9Ôĳĕuĳ65 Hagen-Poiseuille ĳ6 _McŚ#ø365 5FC
$ǗƔ¥Ɖ*!ƯƴĳĔ#Í5ķŴÙ$ßǏiÈ%"24Ǒƍ#Ŏ6 3ƯƴĤġ#
pâƢż"ĞƜçƕ5
Ɖ#2ľƩÃ$ƯƴǄĦ65¥ǚǄĦōǛ%ľƩ$ÃE;H#2¼5Ãŕď
FC 0.66 $Ɣ¥Ɖ%466Da
0ƾă#Ŏ65ŕÃƽ%κ-carrageenan $ƩƽĪFC 0.33 $Ɣ¥Ɖ%327Da
4FC 9å®Ã Ɣ¥Ɖ$Ãŕéƈ9Ɲƛ5 Č3 ""
Ãƽ 500 t
3 j$Ã$ǄĦō% 96~98%ÉƩŚ#Ã$Ưƴ%ő"ĒšūƤƓƉ%Ãƽ 500 tj$Ã#
%.7ƮĵƯƉ œ5 Ť6 6.
ǗĳĔ ÃƣƦĤƈ#25ÃƅƶðǋƉXdIG
Figure 4 #%iƲ$šūžĘ9¾ħÛƲƀƉǋXdIG œ¸¥$;^hF9ŤpâwŚ
"ÉƞÉǖ95çƕ5,T;>őŒň$¾íŷć9~#ǋ5¸¥%jĳ#
%Ǘ
ľÂƯƴĳĔ9Ɓõ5 chitosan Ɖ#2¿"ÃƅǚJfUBƩůǛ9ùŌ5Ēšū%_M
cŚ# Lysozyme 1 BSA $ùŌ#2Ťĥ#lŨÙ$ÃƅǚCcDhG1ƋŻÃǛ9
κ-carrageenan/pullulan ÃE;HƣƦƉ#2ǋ3#Ð"ÃƅǚÒŻ"!Ǜ%ď0kĳ#
ǋ5XdIG5jĳ#ƒǎāƩ9Ċ chitosan Ɖkĳ# κ-carrageenan/pullulan $Ɣ¥Ɖ9
œƉǋXdIG9¾ħ5 #24ÃE;H#2ŕ5 5
Feed
MW (Da)
105
'
BSA
Egg Albumin
Pepsin
Trypsin
Lysozyme
6/0chitosan
Z=]O245.-
3
^)
(Up Stream)
K]R@
K]R@ Pepsin (34KDa)
Egg Albumin (43KDa)
BSA (65KDa)
VZS:Q_[
VZS:Q_[
<M>](290Da)
9JSYU](222Da)
@\\B]%(354Da)
[L](610Da)
104
κ-carrageenan/pullulan
3
E9H24510
Rose Bengal
Brilliant Blue
Bordeaux S
Indigo Carmine
Methyl Orange
102
Glucose
Urea
E9H245+
3)"!$1
&3#T\IG3,
(Down Stream)
(
(
A[C_G (180Da)
W[N_G (342Da)
YS8Q_G (504Da)
;ZD
(300 ~ 3000Da)
7XQ%
*7XQ%
*7YP] (89Da)
*7[?P] (174Da)
*7GRY?] (132Da)
*7GRY?]% (133Da)
*FGM9] (121Da)*
Figure 4 The image of a multi-stage membrane separation process.
7. Ƃô
őwǗÃ$ŶǀRLNehB$å#ıŜĈƗ"Ɣ¥#24ÃƣƦĤƈƉ$ƤƓ $èœ#
šū9Óǁ
Silica æųÃ#25ƿ´#25ƒǎāƩ9ƑǗĳĔ$ÉŎ9¯5
0#Cibacron Blue F3GA 9b@f
O ¿"ÃƅǚJfUBƩůǛ9ƶðŚ#Ɖǎ#ùŌ5 #2jĳ (Up Stream) $ǋĀx
#ŞèǗÃƉ9ƤƓ
κ-carrageenan
pullulan 9Ɣ¥ļŀŊë0ÆÈ"ƊŮƔ¥Ɖ$ƤƓ#íƔ¥Ɖ%ÃE;
H(60~1017Da)9ƾă#ƣƦňƩǋƉ £ƈéƣ/365
0#κ-carrageenan $Ä²¥9å
®Ã ÃE;HƣƦƈ$¼đá5
iƲ$šūíĘ%Ǔ¬ŔĖ$őwǗÃ$Ɣ¥ Å}ǔ#24ŶǀRLNehB$å9ÉŎı
Ŝí$ŉé#èǋĀx$jĳ kĳ966ñÝ5ǗÃƉ9ĈƗ#ƤƓǋĀx$¾ħ
ŚƲƀŚ"XdIG9üě5 5£ƈé9ŤŅ>bFPc5
4

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