【要約】 Comprehensive Analysis of Gene Expression

【要約】
Comprehensive Analysis of Gene Expression Changes in
Mouse Epididymis by Neonatal Exposure to
Decabromodiphenyl ether
（新生児期デカブロモジフェニルエーテル投与によるマウスの
精巣上体における遺伝子発現変動の網羅的解析）
千葉大学大学院医学薬学府
環境健康科学専攻環境生命医科学
（主任：森千里教授）
中本真
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SUMMARY
Decabromodiphenyl ether (decaBDE), a polybrominated diphenyl ether
(PBDE), is the most well known flame retardant that is used worldwide. In a
previous study, we identified adverse effects of neonatal decaBDE exposure on
mouse epididymides, such as decreased epididymal weight. However, the
molecular mechanisms regulating the induction of harmful effects by decaBDE
exposure remain unclear. Thus, we investigated the effects of neonatal decaBDE
exposure on mouse epididymides, and attempted to elucidate the molecular
mechanisms regulating the adverse effects of decaBDE exposure. Epididymides
were histologically examined, and gene expression was analyzed using DNA
microarray and real-time RT-PCR. Our data showed that 1) no histological
change was observed on epididymal tissues by neonatal decaBDE exposure, 2)
decaBDE exposure induced changes in expression levels of some oxidative
stress- and glucolipid metabolism-related genes in mouse epididymides.
Thus,
the alterations in gene expression might contribute to the induction of adverse
effects in mouse epididymides upon decaBDE exposure.
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Nakamoto M, Miyaso H, Komiyama M, Matsuno Y, Mori C
Changes in expression levels of oxidative stress-related genes in mouse
epididymides by neonatal exposure to low-dose decabromodiphenyl ether
Reproductive Medicine and Biology
DOI 10.1007/s12522-013-0173-x (In press)
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