3/.33*3 (#2-+& 1,0+%( 1)$!-"+-" $"O%$QRI5 CXCR4$"%RH2""@( TNF-α7FN 52% $6NR0)53@K D)9K L"9N3K (S-J;D+;CV-*,;7B.W) (/[NI)U*S;32!4*5()-*D-3) ;)-LQ4 OQTPU*/X0I@ <);02(%@<"[QH-'AV ;) ;*@ -TV -3=-T*/ -EH R*S;2 :V[*" -N*N72 <$;S ;*@ (%@<" [QH -'AV -*AV [email protected]. ;);*@ -TV -3=-T*/ -EH R*S;2 :V[*" -N*N72 <$;S ;*@ (%@<" [QH -'AV -*AV D I .;) .-5V(S]=-'AV-5V:6 ![P[*/;%*S;& (/;S S f ;) <);02 U)[V[QH:%AV<);02(%@<"[QH-'AV-3= *)&" o e !1&" 1[E?: [D]v D 3[E}> bz CXCR4 1[]EA 2 L` ~ .}` [E8 .}` CD^BDHS 2 ]SH +[ 2[z TNF-α]EnCJHN|3H DH>NDH`HJH 0=D]WMH[NaE CXCR40HEJJ Hy MSCsCEd^3HEJ3H .}` ]EPHM LWM0Ha0ZN` ^t MSCs CXCR4 0HEJ]EEtM0^Eq|HlD [ CbD^v CXCR40HEJ0^E v i h Lv]A ]yC` ]J TNF-α CJHN|DH>NDH` 3H 12]A21[d HEM 3H .}`[ Hc12]A[[c~EazM[ Hc2 12]A2Jq|Hl MSCs H>#2 H .}`3 mRNA_9`JLpH` 2 3H 0H! 2 ng/ml3H Ln}sHJ TNF-αHJ1[c HEM3H .}` EN>HNJCBHY.]N>06[D]AC` ]J Real Time PCR#2 HJ CXCR4060HEJ0^E 2NYH`0 cDNA 2 ]ZN` c r [c{E}WM2D^TM -∆∆Ct-E|#2 !1HwN`HJ1[d HEM21[c HEM3H .}` 0HEJ]EEtM 4HNDHJ 2 IEM]MJ0HEJ0^ELpH` 2 3H 0H! TNF-α ng/mlHJ1[c HEM MSCs H NvHD CXCR4060HEJ0^ED]NdEJ[c]J]J 2 IEM]MJJHd3H 0H! ng/mlHJ HEM 2]J]J A [D]A1[ Hd ng/mlLn}s 2LpH` 0H![ TNF-αHJ 2HT 0Ha0ZN`^t!xNd MSCs CXCR4060HEJ TNF-αLn}s~ 20H!{Hp~ [` C]nJ 3]EATEN L`H .}`D CXCR4060HEJ]EEtM 3]M~{Hp0H!C|2L`]P 3:3QKS7H α#V4d!$5fE#WDQ CXCR4IJQDD4dfKGIKmg7!QKS7Q1i 3[E}> 3H 162 1i dV I IKmg 7!QKS 7Q 1i [z +S 5self-renew dDdKV#!bfKyIJQ 3bfK~7!QKS7Q1iIKmg7!QKS 7Q 1i I#!5g fK~ 5 I#!5g Qm QS Q 1i Wc dQ Q UQS fJQi 5 3cWi g #! D dWj $Qi 7Q 1i I5fs~ 7Q 1i UiSPWi Ql U\ IJ#D 7Q$f= 5 .QdS 3b ISf@ UQS 5 7dTD7Q 1iR 1QKV5d7Q1iISf@ 7Q 1i Io zT dlQSI DQi 7Q1i 3V dlgJQW ITp{7Q 1i Ui 3cWi g 3QjG#gS #! IKmg 7!QKS 7Q UQS S gJQV 5 fKZEV #! IKmg 7!QKS 1!Q| 3cWi g #! Q 1i J UK|\ QmJQ$ 7fS ITiQ zT D 4dl RTi Wc dlQSI 7 Wj CV 7Q 1i $ do#! www.SID.ir 3HEJ3H .}` CXCR4060HEJ3B|]EEtM TNF-αbzC` ]J JQDWJQiD 4!ncmfKbQ|Qv!!fG 5 KfV #! 4!QWi K@ 5 IQ#! 1i IKQS $ IbfS 5 TNF α IL -1B dQ ISQW 7Q dJQj_S UQSWc+7$Qi$QS 5 α4β1 JfHWJCD44dQ 4dTj@7Q1E 7!d|V IKmg 7!QKS 7Q 1i I>KV fy $ dQHK 5 CXCR4 \ $ IJQDD 7Q 4dfKG fD#QCJ?KD dJQI3QKS#Ii7QfD#Q 1if\QIiQi7g\ CXCL 12 QJ SDF-13 Q 1i J dlQSI Q 1i J IoQpWb Q JQDD dWjQW` SQ \ $ CKWJ=VQ 7!QKS 7Q 1i 7QfD#Q KyVSD dWj I{Q37Q 4dfKG5 5dDI3QKS# CD115 CD14CD34CD45 JQDD3QK#!dD ICDIif\Q CD105 fKy 4dTj@ 7Q 1E IbfS SQ #! 1i Homing 5f\Q#!Ik SDF-1 Q CD73 (SH3 / 4 Thy-1 CD90CD44(SH2) !d|W Q|Qv #! dD I 7$QS 7!QKS 7Q CD116 CD106 (VCAM - 1Stro - 1 CD71 #!IKmg7!QKS7Q1iDUi4dl&#gG dD I 3QKS # CD29 5 ICAM-1(ALCAM dDIf\Q SDF-1 QJ CXCL12SaiQ= 3{ S CD200 5 CD271 GD2 8fKb 4dfKG 4dl WbQl dQHK QV CXCL12 g IKmg 7!QKS 7Q 1i 'Qb 7QfD#Q J# dlQSI GKPV5f=SpWIJQm~ IAK=#Q D d4!fD&#gG7|Qv#! k#QE5 v i h tiV3Qj.QdS3b7!QKS7Q 1if\Q 5 W= !l I 7fGI]QK SDF-1/CXCR4 7Q 1i CJf`V D d!! 3Qm k#QE c r D I S f o e U 4dfKG CJ D Ui CXCR4 IJQDD 7$Qid\ d 4dl WbQl 3cWi #! UDfl 1i + Jd@ S gJQV IJQV 3Qi Ib JQj!\52d{5IJWjKi 7QkD5 $ 4!QWi S # 3dm! \V D dWj IJQJg R\ IKfVIElg=#! IKmg7!QKS7Q1i 57dKD IGBJ5CJfHJ!7i$ Ui4!fD 5 SDF-1SaiQ= TNF α ISQW JQDWJQiQS7!QKS KfV #! UDfl 7fS 7!QKS 7Q 1i #QKjS -d!IkJg# Q1iJ f\Q UKSQ #! 4dJ!RKi7QUQSS f\QIJQV UQS - TNFαDUi4!!3Qm 7fHJ!|Qv K@ Ui 3dSb!#!5IQHmJQ$tJfl -I kJg # IKmg 7!QKS 7Q 1i IJgG IIKmg 7!QKS7Q1i !\dl%QifS 7Q JQDWJQi WfG 2Q] Q|Qv fSQS d! QJ 4dJ! RKi 7Q UQS 4BJ5 S Q UQS #! dV 5 Q JQDWJQi fJQi 3QKS 75# fS IV5QW fY ISQW 1i Homing dJf jKQE dl DQi RW -I IKmg 7!QKS 7Q 1i f\Q UKoQb S 2KV5d $ #eG 5 UQS S IKmg 7!QKS 7Q fKYQV Ii#fS |Qv J $ .d d#eG Ddi#IfySQ Ui4dmWbQl QD#u 3: 3QKS 7H fS TNFα ISQW 7QJQDWKi 7QdQHK QJ 5 Q 4dfKG 4dJ! RKi 7Q UQS 7!QKS 7Q 1i #! CXCR4 IJQDD 4dfKG 5 f\Q dJf D dJQI 3QKS # IoQb Ui 3Qj3cWig$WmIKmg ` S # IKmg 7!QKS 7Q 1i Homing H #2 2 S KTl 7dJf 8QW_ 5 4! KjV U_f\ CHa3HEJ3H .}`Ld>23!H`[S -IR\ RW7QUQSS Q UKiD f\Q A www.SID.ir !-"+-"3/.33*3 (#2-+&1,0+%( 7gK F# tiV gJg:PWi 8QWJQ dJ!fGI UJQr#eb$h=IQjIKmg7!QKS7Q1i SgJQV#ySUfG#fIi#fS!# !#J#gK 4!5d` #! Qi 4d! !f H 3cWi $ Q #!W dS #Q@ :QiQ=7Q 1iISf@UQS 54fK>i$fKl7$Q3QWi#QKS#!1Qi Ii UmD tK` dWfG #f CK:>J! UmD tK` dK= Q K `V gDf 7!QKS 7Q 1i 4QHmJQ$ S 35gKV#D5#dK mmol/L Ql CK:>J! tK` mg/L QS f dJ!fG W Qs{ 5KiQW5dJ gr/L CKS#EidKi gr/L CV7Q1idlK# UTjS DMEMUmD UmDtK`qJ|V!S35$QWgG! mol/L dld\QJfJQi$1EJQ$4!QWiQS7Wj F# tiV gJg:>J! h>i dl 2Q] W #! #QS dJ!fG :KJfWQi 5 Qr UmD tK` h>i .UfG#fIi#fS!# Oil Red O7gK do#! 75Q_UmDtK`#!oQ_3KjQ>ii 3]NNDH`}v UmD UbEJ #u S T25 Ci #! FBS 2fi 7!QKS7Q1i7fWWJQiCKEV$4!QWiQS do#! 5!fGIWQi\#! 7Q!#!5dl4!! 2i:QiQ=7Q1i$#QDJ7fSddlIJQiQl 3dKTj@QS $5# $h=dlSESfDdKjD7! Q1idWSdl4!QWido#! $kKSDfVQS 1i 5 ({ UmD tK` .fx D S Q 1i _f ddl 4!! #f !fG IWQi \#! 7Q! #! 1ih>idJ!fG#fEV8!d]:KJfWQi5mWjl v i h IWSpWFITCUi#QQKiKV5gJQSQ CD31 CD34 CD11b CD45 7Q 7!QS S K! d S 5 #!3Qm CD166 CD105 c r S f o e K! dS5dJ!fG:JfWQih>i5Kj>JfV D I QS$5# f3$h=dJ!fGd\4dTj@fK~7Q phosphate buffer salin PBS 7mWjl 1` Q1iD$5# $h=dlIqJ|V5mWjl :QiQ= K5 ddKi# do#! 2$ DfV S #5! UmD tK` dWS Ii :QiQ= 7fS dl 2Q] h>i4!!mWjlmWjl1`QSQ1i5WcJ# ddl 4!! #f CJ#QV tK` #! 1fWD 4f \#! 7Q!#!IVd5QrKj>JfVQ1iS 5 4!! mWjl fQS QS 8QD #!3Qm 7Q 1i $ 4dl d\ 7Q 1i ddl Wl! H !fG IWQi dKOd 2f#Q= QS IJ5# 1`ddJ!fG :KJfWQi 7W` 7Q Ci S 5 7#5 z\ .fx D Ii#fS!#7fWWJQi 4QHWi!#!5 UKTZV do#! 7Q:QiQ= $5# f 5 W 4$QV UmD tK` S 1fWD <JQV5gJ 7Q 7!QS IW $ UfG #f dJ!fG2Q]:QiQ= QVIW A 4!QWi IoQpWb fK~ 7Q kD5 .e_ #y 0ZN`2CJ]:LvHJJ^DHM dl IKmg7!QKS7Q1igJQV3VIi#fS#yS CEd^3HEJ3H .}` HEM :QiQ=7Q 1i$ISf@UQS5IcWiUQSS DfV QS 2#Q@ :QiQ= IKmg 7!QKS 7Q 1i 2fi 75Q_ UmD tK` #! Cells/mL 3Q$ #! ng/ml5 ng/ml7Q$5!#! TNF - αQS $h= ddlSE U{Qi 5 7Q Q 1i 4dl WfGfy #! 7Q 3Q$ d UleG tK` #! W d S Q 1i dl 4!QWi 75Q_ tK` J ddl 4!! #f CK:PWi UmD 15fjKG mmol/L 35$QWgG! mol/L dKi gr/L JdiQSfDIS gr/LQj qJ|V UmD tK` W #! #QS !S CKS#Ei www.SID.ir 3HEJ3H .}` CXCR4060HEJ3B|]EEtM TNF-αbzC` ]J :KJfWQi K! dS!fGIWQi\#! 7Q! IE kJQ$ _f 2Q] 7fS 5 7#5 z\ 3 S !fi 1Q=5f=5gJ µL 5 d\ IS $Q 5 Real CKEVQS CXCR43:3QKS3gKKK|VU\ U{Qi !fG IWQi \#! 7Q! #! 5 4!5g 3:3QKS3gKdWfG#f4!QWi!# Time PCR :KJfWQiQdJ$d|SdJ!fG7#dH -5 4dm#QKV 54dl#QKV7Q1i#! CXCR4 Q 9KV5fEK 35#! 1` KcV $ h= ddl 7Q 45fG #! CXCR4 3QKS ^vi #! 5QV K@ #! #5!QS5QrQ3S!fi1QV µL 5$5!#! TNF - αQS#QKVU`VDIiWc :KJfWQi K! dS!fGIWQi\#! 7Q! Ii#fS !# d!S WfG #f Wc 7Q 3Q$ 54!5g3S DEPC µL3$h=ddl UfG#f 4dlfcWi mRNA$ cDNAgWidJ!fG)c CXCR4060HEJ3B|^E|H 7Q 1i#! CXCR4 3: 3QKS ^vi Ii#fS U\ M- %E| #!fS cj Jg µL dNTPs µL #d dWS#!dl4!QWi2#Q@:QiQ=7Q1i$ fv µL $QPDTJ# 4dD #Q µL o e µL IJQ ]_ QS mRNA µL 5 fQS µL UfG#o iv h c !# PCRdJf#!1EK= Uy~QS 5I_fu 15d\dWfG#f4!QWi r A S f TNF - αQS4dl#QKV54dm#QKVIKmg7!QKS Moloney Murine Leukemia Virus Mulv cj HK5 5 NCBI $ Blast 7Q#g 2f $ D I cDNA^N`2 mRNA]ZN` fgH25d#fJf= µLQlITKDfV$4!QWiQS Real Time- PCR 4!QWiQSKWDQWS3:5 CXCR43:7QfJf=dWS 7!QKS 7Q 1i S PBSmWjl fQS µL RNX fcWiUKD$4!QWiQSdlQr IKmg mRNA|#Wi!%QifS53:QKi Plus !S fl J S #QD &5# dJ!fG fcWi Ii 3KjQ>ii µL S RNX Plus µL #d #d oQ_ )c S dl )c 5 Qr Ii #! #5!QS5dJ!fGQr!fi2f5fD µL EN>HNJ2 CXCR4063H ]D]8HgZd .2[S 06 CXCR4 KWDQWS 1[]J}S]D]8 "?q]D]8 5`-GGA CCT GTG GCC AAG TTC TTA GTT-3` 5`-ACT GTA GGT GCT GAA ATC AACCCA-3` 5`-GGG CGG CAC CACCAT GTA CC-3` 5`-GAC GAT GGA GGG GCC CGA CT-3` CXCR43:7fS RT-PCR2Q]U\fEJQifV QWS5 CXCR4 3:3QKSJfGfTJQi&5#$4!QWiQS \#! K! K5 3Ki#VQ! $ !S #QT{ U\ 4dl 4!QWi PCR RKDfV dl Ii#fS KWD IWQi\#! KQY JQY3Ki#VQ!!fGIWQi !S fl J S CXCR4 3: 3QKS 3gK Ii#fS \#! KQY 7$Qi Ju 5 1QpV _f !fG fJf= µL fv µL F# µL 3: 7fS 4QHWi! QfS EKi #! !fG IWQi 5cDNA µL%E|fJf= µL54dfS\ \#! K! K5 3Ki#VQ! Ql KWDQWS 3:3QKSIi#fS7fS PCRRKDfV5hEK4f= µL \#! KQY JQY 3Ki#VQ! !fG IWQi fv µL F# µL Ql KWD QWS KQY 7$QiJu51QpV_f!fGIWQi %E| fJf= µL 5 4dfS \ fJf= µL !SEKi #!!fGIWQi\#! 4QHWi!QfS!ShEK4f= µL5cDNA µL www.SID.ir !-"+-"3/.33*3 (#2-+&1,0+%( d Ui! S 4dTj@ J CJ $5# d|S 5 ddl 3 H{E}WM UiS5fTK 7Q ID IVd $ h= El f 7fS Real Time PCR &5# $ 4!QWi QS ElddQl= # .fx D 5 4dl KEmV Ui!S CtdUi!SCycle thresholdCtCJ 7Q 1i JCJU{fiS3cWig7Q1i 3:7fS4dUi!S Ct 4fSQ $4d 1iJdDIdKV ElUiS5fTK 5CJ#QS dlgKQfJ$1ftiVKWDQWS ∆Ct(experimental) = Ct (Treated MSCs) - Ct (β- Actin) ∆Ct(control) = Ct (Untreated MSCs) - Ct (β- Actin) ∆∆Ct = ∆Ct (experimental) - ∆Ct (control) r = 2-∆∆Ct 5;#gS7Q1i dWj1i +5!QlQ fVC@D 5 El ID5! 7Q 1i + ^vj kJg Q 1i 4$d 3Q$ UleG QS El RDHN D I 7Q UK QS I|r d@ 7Q 1i S 5 WQJ C|}`Ld>RDHN 4#5! Iu #! 5 ddl JdTV i=WKi #! ncm $5# 7Q 1i %E| 9Ei5fEK CD S d!fD w_ # !b dQ UiS5fTK fQx dl# $ $5# UleG $ h= dWfG #f Ii#fS !# S f 3QJQ.fxD#! 4dTj@ 7Q1iK5UmD v i h o e c r D!2 Lw HM !_8u|0Ha0ZN`^t!xNdCEd^3HEJ3H .}`Ld>23!H`[S {?c 1[D83H .}`2[H-2.}`(2] Ld> [HL`J2]KEv3H .}`0[c] Hm1[Ka: A um"HEz[[c 0[H1[!LE}JHyCJHD! fD#Q 3QKS S UTj D IQ_ #! ! #! 7Q1i!d|V5dl&#Ql Q 1i:QiQ=f#! $4 #! d!SUTZ IQj CD166 5 CD105 #fISQJ$#!#S3Q>JfV7gKF#tiV4d$ IKmg7!QKS7Q1i!\54dUi!S[JQW d!S4d$Q do#! 5 KSDdWfG fS 7fWWJQi [JQW K`V 5 Jg]V !fD QTY # 3]NNDH`}v{E}WM2D^TM IW #! QS <JQV5gJ #! !#bfS v %Qi UfG#oIoQpWb7!QS 7!QKS7Q1iD!!3Qm7fWWJQi gKQ[JQW fD#Q 3QKS S UTj 3Qj 3cWi g IKmg dWjI CD315 CD34CD45CD11b www.SID.ir 3HEJ3H .}` CXCR4060HEJ3B|]EEtM TNF-αbzC` ]J S f D I 0Ha0ZN`^t!xNdCEd^3HEJ3H .}`3H ]> H CDC` ]J JLKQCHa CD1662 CD1053]J2CwCD312 CD34CD45CD11b3]JRDHN o e 1i ISf@ S Q 1i gJQV 7fS El CK:>J! UmD tK` #! IKmg 7!QKS 7Q $I~7QOD5z]VW $h=ddlSE v i h 7Q OD5 UJQ #! dl 4dJ! Q 1i #! ISf@ EldlISf@$f=1i5ddlRKDfVISf@ c r A CJ]:20ZN`^DHMRDHN #!QUiSPWiSIKmg7!QKS7Q1i gJQV 7!QKS7Q1i3!fD SEQSIQHmJQ$tJfl W dSCK:PWi UmDtK` QSJCV #!KjDz]V7!QKS7Q1i #! UfG#o !# J#gK 7gK F# dl I 4dJ! 3Q$ 1u !fD dKJQV # Q UKiPWi #! KjD i# !\5 1HBdDH!jEW 0Ha0ZN`^t3HEJ3H .}`^DHM {?c D ^E|3^E@ HJ0ZN`J^DHM[c/HT/ H:6H`H8![qJ CJ]:20ZN`JCEd^ 3HEJ3H .}`^DH µm"HEzNvHD^DHM.}` CJ]:3H 1]lyu|JLKQ www.SID.ir !-"+-"3/.33*3 (#2-+&1,0+%( ∆∆Ct #d ng/ml 3: 3QKS IE kJQ$ $ oQ_ [JQW %Qi fS 5 = ∆∆CtRKVfVSUl!7!QJ$kJgU{Qi 54dm#QKV IKmg 7!QKS7Q1i #! CXCR4 [JQW D jJQ #! fHJ! .fu $ = ∆∆Ct ^vi !S 5QW fHJ!CJ QS TNF-α QS 4dl #QKV #! CXCR4 3: 3QKS ^vi JfVQS D dl 4dQm #QKV IKmg 7!QKS7Q1i #! CXCR43:3QKS dWI/QVU{Qi #! TNF-α ng/ml QS #QKV |QvJ#!4dl#QKV7Q45fG $fWD 4dm 15d\#! = ∆∆Ct #QKV7Q45fG#! CXCR43:3QKS3gKjJQ !S #QKV 3Q$ d #! 5 ng/ml TNF-α $5! 5! f #! D !! 3Qm 4dl c r S f o e v i h D I 1[c HEM3H .}` CXCR4060HEJ]J TNF-αLn}saDHz HbD^v mRNAUl` CXCR40HEJ TNF-α ng/ml~ 2 ng/ml [z~ CEd^3HEJ3H .}` A bD^v]J]J2HM ng/ml [z!bEJ CXCR40HEJLpH` TNF-α ng/ml 3]EATEN2 OWJ IKmg 7!QKS 7Q 1i Q WQJ %Qi fS d -VQ 7!QKS 7Q 1i homing jKQE 4$5f VEGF PDGFdQ Q #WDQ $ IbfS S IQj J 7dKD 7Q4dDKyV 53cWigSCKWJ= BMP-43cWiCK:#KPV5f=IL-8IGF-1 homingdJf!##!Q Ui4dlWbQldJf d!I 74BJ5 IjDQVD aiQ= BMP-75 7!QKS 7Q 1i engraftmentIJgG 5 J homing 5f\QKjQEd@f !#! !\5 7gKHfS X`S 5 D Q{u IKmg IfySUi4dmWbQlIWi#!S$Q1i 1i f\Q75#fS D 7!d|WQ|Qv 7!QKS7Q1i homing 5f\QdJfDdi# QW`QJ5 RKi `S IKmg7!QKS7Q tethering_fi#!QUKiDSKTlIKmg 1i tiV D CKjDQVD { S WfG 2Q] 5!fQdWI/QV transmigration5 rolling Wl!\V5 4dlgDfW !lIdKV IJ7Q www.SID.ir 3HEJ3H .}` CXCR4060HEJ3B|]EEtM TNF-αbzC` ]J S5dD4!QWidVI tetheringjKQE3{ -CJQSf\Qd5#fKG#!7Q1E#!dJf CD44fKyfHJ!4dTj@7Q1E$Q37Q\ 7!QKS 7Q 1i 1QZ 3{ S d#! 5QV fHJ! dDI4!QWi VCAM5 VLA-4 -I3QKS# 5 $fjfVJ$DJgIKmg S P5 E KWEi7Q4dfKG$K!KS5dD bDH! 12]A 2[ Hc12]A bDH!TEN .2[S TNF- ng/ml[ CbD^v TNF-α!220E`HJ?0H!JNaJ2CEd^3H .}` CXCR4060HEJ TNF-α 3HEJ3H .}`HJaDHz ]J]J 2 IEM]MJ CXCR4060HEJLpH` 2 0H![ α IEM]MJ CXCR4060HEJLpH` 2 0H![ TNF-α ng/ml bD^v1[d HEMCEc^ bD^v1[d HEMCEc^3HEJ3H .}`HJaDHz ]J]J 2 HEMLEqh2 Ct CXCR4 Ct β-actin ng/mlU{Qi – ng/mlU{Qi – ng/mlU{Qi – ng/mlU{Qi – D I ∆∆Ct 2-∆∆Ct ng/mlU{Qi – #QKV35dS7Q1i - - r A ng/mlU{Qi – ng/mlU{Qi – iv h c ng/mlU{Qi – S f o e ∆Ct 7!QKS 7Q 1i #! !#! IJgG 5 f\Q 7Q1iIKmg7!QKS7Q1i fHJ!.fu$ dQm UfG #f K`V 5 Jg]V !# IKmg 5dQIQ\SQ;fJfWjS#!DdWjIG#gS g IKmg 7!QKS 7Q 1i D ! ncm S aiQ= #! D d! I 4$Q\ Q3 S Upb J J dDI3QKSD#QKjS # CXCR4 3Qj3cWi 54!fD#T{/5f{1QKV5dQm~$IJQDDRKl 5 J5 Ui 4dl dKJQVgK fHJ! Q|Qv#! ]KW 3QKS fKKV 7H |Qv J #! dl UQS !#5 $ IE@DkcS Dd4!fD&#gG 3#QE #! I k D CXCR4 IJQDD 4dfKG 3: www.SID.ir !-"+-"3/.33*3 (#2-+&1,0+%( TNF- tiV D 4d|3Qufi7Q1i#! CXCR4 5 3QKS # CXCR4 IKmg 7!QKS 7Q 1i 7# K@ dSQJ I kJg d 4dl Q α CD IQHmJQ$tJfl #! Q1iJ f\Q 5 Q JQDD IFN -1β 5 IFN -γ D !! 3Qm $ $fWD D dl4!!3Qm |QvJ#!dDI 7!QKS 7Q 1i #! # IJQDD 7Q 4dfKG I 3QKS# CXCR4 IQjIKmg 7!QKS7Q1i dDI KyV 3QKS kJg Ui S IQj IKmg 3QJd_ tiV D 7fHJ! |Qv #! WQJ J dD 3QKS D d!fD 4dQm k#QE 5 45 d!!3QmQ3 dJ!fGdKJQV k#QE5 QKD 4dl #QKV IQjQKG5fWi7Q 1i#! CXCR4 3:3Qj3cWigIKmg7!QKS7Q1iD 4dQmd@f dSQJIkJg IL -1β5 TNF- αQS fK~QJ5D mRNA^vi#!# CXCR6 5 CXCR4 #! # IJQDD 4dfKG 3QKS TNF-α D Ui 4dl 5 Sordi fHJ! .fu $ dD I 3QKS nKcmV SQ 5 GQi d! I kQD Q 1i + $ Is|S 7Q 1i $ D d!! 3Qm k#QE CXCR23QKS TNF-α Dd!fD4dQm3#QE dD I 3QKS # CXCR4 3Qj 3cWi g 7!QKS d!IkQD #PD# I=7QUKiD#! 7Q 4dfKG 3QKS 3gK D di# I fy S JfSQS 3QKS TNF-α D d!! 3Qm k#QE 5 3Q X`S IKmg 7!QKS 7Q 1i #! IJQDD IKmg 7!QKS7Q1i #!3:3QKS7Hd!I 7Q 1i 5 Q UKiD Q UKi5fWi dQ v i h gJ#5JQDWJQiWc7Q$5! fKYQV U`VIQufi dVI{J dSQJI fKKV IvK`7Q#WDQ 1i #! Q JQDWKi IQHKi #QmS UfmK= X{QS c r S f o e kQD & K5 7Q UKi5fWi #! # CXCR4 D I 5 dQHK k S\VQS UigKHfS IJgG 5 f\Q #! SDF-1/CXCR4 4dfKG #! CXCR4 IJQDD 4dfKG 7QS 3QKS Ii 7fS# Q3 IJgG 5f\Q IJQV Q1i 7BVfWi CJ dV I 5 kJg IKQS 7Q !fS#QD dlQS IQ#!1i #!Q 1i KjQW= 3!fSQS7fS 7Q3: 5 !SdbfY3:3QKS7HfSDdl 7Q JQDD 5 ISQW 7QJQDWJQi fHJ! 7i$ IJQDD 7Q 4dfKGQJQDD\$ Wc dVID!#!!\5 4dJ!RKikcS#! IWc 7Q#WDQfSfKYQVJfu$ #4dTj@7Q1E5 7!QKS 7Q 1i UDf_ UJd 5 !Q]J X{QS $ kK= dD I KyV IjJ5# !l RW 54dJ!RKi 7QUQS S IKmg 7Q 1i k#QE 5 RD frQ_ |Qv 7Q1i#!# 3:3QKS dVI QJQDWJQi J ng/ml 5 ng/ml ng/mlQS#3dcV3Qufi dD KyV 1i gJQV _f 5 + S WjS Wc 5#QKV 3KiQSE 5QW7Q 3Q$#! TNF-α I D Ui ISQW JQDWJQi CJ TNF-α #! CXCR4 3QKS 3gK JfWmKS D dJ!fG ncm 7Q 1E 3QKS 5 1i #! 3: 3QKS dV U{Qi 3KiQSE3Q$d5 TNF-α ng/ml d! #ffKYQVU`V#f\Q dJf#!fKG#! #WDQ KS D d!! 3Qm K@ Ui 4!! # #! CXCR4 3: 3QKS D Ui 4dl USQY 8fKb IuQTV# CXCR43QKS5 TNF-α5 NF- kBIjJ5# HGF fKyJQWDD7QJQDWJQiQSDIJ Q 1i TNF-αD dl4!!3Qm|Qv J#!!#!!\5 I kJg d 4dl #QKV SDF 5 dQHK FLT - 3 IKmg7!QKS7Q1i#! CXCR43QKS dVI 3QKS D d!! 3Qm 3#QE 5 O: dSQJ A www.SID.ir 3HEJ3H .}` CXCR4060HEJ3B|]EEtM TNF-αbzC` ]J #! dV I 4QHmJQ$ #! 3Q$ fy $ 5 $5! $5! 5 3Q$ S WjS5 3Qj 3cWi g $ Wm 35fK= 5 IKmg 7!QKS7Q1if\QUKSQ 5 ng/ml $5! 5! f #! TNF-α d! kJg JQ=fS7Q 3Q#!#!7!QKS7Q1i$4!QWi#! mRNA^vi#!# CXCR43QKSdVI ng/ml dlQS1idK= #d #! CXCR43:3QKS^viJfVQSd!kJg C [y2]?dM fSfS 5! QV CXCR4 3QKS D !S TNF-α ng/ml 7#QE 7fS IJd { Kj_ Ubd fWD!$fEmVQS S 7Q WQJ !! kJg TNF-α ng/ml $ kKS $ fEmVQS K@57fWWJQi K`V5Jg]V #! TNF-αQS #QKVv3Q$D !!3Qm 4dUi! IKQS Q K `V |iV gDf #! #=fEl Jfj fWD! WQJJ!SU{Qi CXCR4 3QKSkJg7fS Q KyV #! 7#QE 7fS 7$Q 3QWi#QKS fy $ IvK` { 1fWD D d! I 3Qm Q 1.Ahmadian Kia, N., Bahrami, AR., Ebrahimi, M., Matin, MM., Neshati, Z., Almohaddesin, MR. (2011). Comparative analysis of chemokine receptor`s expression in mesenchymal stem cells derived from human bone marrow and adipose tissue. J Mol Neurosci, 44; 178-85. 2.Asagoe, K., Yamamoto, K., Takahashi, A., Suzuki, K., Maeda, A., Takano, K. (19982012). Down-regulation of CXCR2 expression on human polymorphonuclear leukocytes by TNF-α. J Immunol, 160; 4518-4525. 3.Ayatollahi, M., Soleimani, M., Geramizadeh, B., Imanieh, M.H. (2011). Insulin-like growth factor1 (IGF-I) improves hepatic differentiation of human bone marrow-derived mesenchymal stem cells. Cell Biol Int, 35; 1169-76. 4.Ayatollahi, M., Soleimani, M., Geramizadeh, B. (2012). Conditions to improve expansion of human marrow-derived mesenchymal stem cells based on the rat samples. World J Stem Cells, 26(4); 1-8. 5.Brooke, G.,Tong, T., Levesque, J.P., Atkinson, K. (2008). Molecular trafficking mechanisms of multipotent mesenchymal stem cells derived from human bone marrow and placenta .Stem Cells and Development, 17; 929–940. 6.Bühring, HJ., Battula, VL., Treml, S., Schewe, B., Kanz, L., Vogel, W. (2007). Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci, 1106; 262271. 7.Chamberrlain, G., Fox, J., Ashton, B., Middleton, J. (2007). Concise review: mesenchymal stem cells their phenotype, differentiation capacity, immunological features, and potential for homing. Stem Cells, 25; 2739–2749. 8.Croitoru-Lamoury, J., Lamoury, FM., Zaunders, JJ., Veas, LA., Brew, BJ. (2007). Human mesenchymal stem cells constitutively express chemokines and chemokine receptors that can be upregulated by cytokines, IFNbeta, and Copaxone. J Interferon Cytokine Res, 27; 53-64. 9.Delorme, B., Ringe, J., Gallay, N., Le Vern, Y., Kerboeuf, D., Jorgensen, C. (2008). Specific plasma membrane protein phenotype of culture-amplified and native human bone marrow mesenchymal stem cells. Blood, 111; 2631-2635. 10.Fu, X., Han, B., Cai, S., Lei, Y., Sun, T., Sheng, Z. (2009). Migration of bone marrowderived mesenchymal stem cells induced by tumor necrosis factor-alpha and its possible role in wound healing. Wound Repair Regen, 17; 185-91. 11.Gangadhar, T., Nandi, S., Salgia, R. (2010). The role of chemokine receptor CXCR4 in lung cancer. Cancer Biol Ther, 9; 409-16. 12.Gronthos, S., Franklin, DM., Leddy, HA., Robey, PG., Storms, RW., Gimble, JM. (2001). Surface protein characterization of human adipose tissue-derived stromal cells. J Cell Physio, 189; 54-63. 13.Han, Y., Wang, J., He, T., Ransohoff, RM. (2001). TNF-α down-regulates CXCR4 experssion in primer murin asttrocytes. Brain Research, 888; 1-10. 14.Hemeda, H., Jakob, M., Ludwig, AK., Giebel, B., Lang, S., Brandau, S. (2010). Interferon-gamma and tumor necrosis factor- A c r v i h o e S f D I ﻣﻨﺎﺑﻊ www.SID.ir !-"+-"3/.33*3 (#2-+&1,0+%( alpha differentially affect cytokine expression and migration properties of mesenchymal stem cells. Stem Cells Dev, 19; 693-706. 15.Igura, K., Zhang, X., Takahashi, K., Mitsuru, A., Yamaguchi, S., Takashi, TA. (2004). Isolation and characterization of mesenchymal progenitor cells from chorionic villi of human placenta. Cytotherapy, 6; 54353. 16.Ji, JF., He, BP., Dheen, ST., Tay, SS. (2004). Interactions of chemokines and chemokine receptors mediate the migration of mesenchymal stem cells to the impaired site in the brain after hypoglossal never injury. Stem Cells, 22; 415-27. 17.Kollar, K., Cook, MM., Atkinson, K., Brooke, G. (2009). Molecular mechanisms involved in mesenchymal stem cell migration to the site of acute myocardial infarction. International Journal of Cell Biology, Volume 2009, Article ID 904682, 8 pages. 18.Kulbe, K., Hagemann, T., Szlosarek, PW. (2005). The Inflammatory cytokine tumor necrosis factor-a regulates chemokine receptor expression on ovarian cancer cells. Cancer Res, 65; 10355-10362. 19.Li, L., Jiang, J. (2011). Regulatory factors of mesenchymal stem cell migration into injured tissues and their signal transduction mechanisms. Front Med, 5; 33-9. 20.Martinez, C., Hofmann, TJ., Marino, R., Dominici, M., M.Horwitz, E. (2007). Human bone marrow mesenchymal stromal cells express the neural ganglioside GD2: a novel surface marker for the identification of MSCs. Blood, 109; 4245-8. 21.Minguell, JJ., Erices,A., Conget, P. (2001). Mesenchymal stem cells. Exp Biol Med (Maywood), 226; 507-20. 22.Musia, RA., Bekchanova, ES., Sukhikh, GT. (2005). Comparsion of mesenchymal stem cells obtained from different human tissue. Bull Exp Biol Med, 139; 504-9. 23.Nombela-Arrieta, C., Ritz, J., Silberstein, LE. (2011). The elusive nature and function of mesenchymal stem cells. Nat Rev Mol Cell Biol, 12; 126–131. 24.Oh, JW., Drabik, K., Kutsch, O., Choi, C., Tousson, A., Benveniste, EN. (2001). CXCR4 chemokine receptor 4 expression and function in human astroglioma cells. The Journal of Immunology, 166; 2695–2704. 25.Ortiz, LA., Gambelli, F., McBride, C., Gaupp, D., Baddoo, M., Kaminski, N., Phinney, DG. (2003). Mesenchymal stem cell A D I S f o e v i h c r engraftment in lung is enhanced in response to bleomycin exposure and ameliorates its fibrotic effects. Proc. Natl. Acad. Sci. U S A, 100; 8407-11. 26.Pittenger, MF., Mackay, AM., Beck, SC., Jaiswal, RK., Douglas, R, Mosca, JD. (1999). Multilineage potential of adult human mesenchymal stem cells. Science, 284; 143-7. 27.Ponte, AL., Marais, E., Gallay, N., Langonne, A., Delorme, B., Herault, O. (2007). The in vitro migration capacity of human bone marrow mesenchymal stem cells: Comparison of chemokine and growth factor chemotactic activities. Stem cells, 25; 1737– 1745. 28.Ryu, CH., Park, SA., Kim, SM., Lim, JY., Jeong, CH., Jun, JA. (2010). Migration of human umbilical cord blood mesenchymal stem cells mediated by stromal cell-derived factor-1/CXCR4 axis via Akt, ERK, and p38 signal transduction pathways. Biochem Biophys Res Commun, 398; 105-10. 29.Shi, M., Li, J., Liao, L., Chen, B., Li, B., Chen, L. (2007). Regulation of CXCR4 expression in human mesenchymal stem cells by cytokine treatment: role in homing efficiency in NOD/SCID mice. Haematologica, 92; 897–904. 30.Shi, Y., Hu, G., Su, J., Li, W., Chen, Q., Shou, P. (2010). Mesenchymal stem cells: a new strategy for immunosuppression and tissue repair. Cell Res, 20; 510-8. 31.Sordi, V., Malosio, ML., Marchesi, F., Mercalli, A., Melzi, R., Giordano, T. (2005). Bone marrow mesenchymal stem cells express a restricted set of functionally active chemokine receptors capable of promoting migration to pancreatic islets. Blood, 106; 419–427. 32.Sylwia, B., Danuta, J., Marcin, M. (2006). Mesenchymal stem cells: characteristics and clinical application, 44; 215-30. 33.Ugo De, G., N.Cohen, E., Gao, H., Mego, M., Lee, BN., Lodhi, A. (2011). Mesenchymal stem cells expressing GD2 and CD271 correlate with breast cancer-initiating cells in bone marrow. Cancer Biology & Therapy, Landes Bioscience, 11; 812-815. 34.Wynn, RF., Hart, CA., Corradi-Perini, C., O’Neill, L., Evans, CA., Wraith, JE. (2004). A small proportion of mesenchymal stem cells strongly express functionally active CXCR4 receptor capable of promoting migration to bone marrow. Blood, 104; 2643-2645. www.SID.ir 3HEJ3H .}` CXCR4060HEJ3B|]EEtM TNF-αbzC` ]J 35.Zhang, A., Wang, Y., Ye, Z., Xie, H., Zhou, L., Zheng, S. (2010). Mechanism of TNF-α-induced migration and hepatocyte growth factor production in human mesenchymal stem cells. J Cell Biochem, 111; 469-75. 36.Zhao, C., Lu, X., Bu, X., Zhang, N., Wang, W. (2010). Involvement of tumor necrosis factor-a in the upregulation of CXCR4 expression in gastric cancer induced by Helicobacter pylori. BMC Cancer, 10; 419. A o e v i h D I S f . c r www.SID.ir
© Copyright 2024 ExpyDoc
