血清中のバルビツール酸系薬物 Barbiturates in human serum F100621B 1. A) Standard (10 µg/mL) O H N O H3C mAU O 2. O H3C O H3C O Pentobarbital H N O 5. O H N 2 H3C NH O 3 4 5 Secobarbital CH3 3. O N H3C 0 H3C O H3C Phenobarbital 5 O H2C NH 10 O NH Barbital 1 H N H3C NH CH3 15 4. O NH F100531A 0 2 4 6 min O Hexobarbital B) Spiked human serum Solid-phase extraction method mAU YMC Dispo SPE C18 100 mg/1 mL Condition 2 mL methanol 2 mL water 15 1 10 Load 2 500 µL spiked human serum solution (each 10 µg) 5 3 4 5 Elute 500 µL methanol/water (85/15) 0 F100531B 0 Column Eluent Flow rate Temperature Detection Injection 2 4 6 min : YMC-Triart C18 (5 µm, 12 nm) 50 X 2.0 mmI.D. : A) 20 mM HCOONH4-NH3 (pH 9.5) B) methanol 0-90%B (0-7 min) : 0.2 mL/min : 25℃ : UV at 240 nm : 1 µL Dilute 500 µL 20mM ammonium formate buffer (pH 9.5)
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