[PDF] pGEXベクター選択ガイド（比較表）

3
タンパク質の発現と精製
www.gelifesciences.co.jp
これらのベクターの全塩基配列は、弊社Webサイトにて公開しております。
3-161
Glutathione S-transferase Region
tac promoter-10
tac promoter-35
lac operator
Ribosome binding site for GST
Start codon (ATG) for GST
Coding region for thrombin cleavage
Coding region for factor Xa cleavage
Coding region for PreScission Protease cleavage
Coding for kinase recognition site
Multiple Cloning Site
β-lactamase (Ampr) Gene Region
Promoter-10
Promoter-35
Start codon (ATG)
Stop codon (TAA)
LacI q Gene Region
Start codon (GTG)
Stop codon (TGA)
Plasmid Replication Region
Site of replication initiation
Region necessary for replication
Sequencing Primers
5' pGEX Sequencing Primer binding
3' pGEX Sequencing Primer binding
GenBank Accession Number
pGEX-2T
28-9546-53
pGEX-4T-1
28-9545-49
pGEX-4T-2
28-9545-50
pGEX-4T-3
28-9545-52
pGEX-5X-1
28-9545-53
pGEX-6P-1
28-9546-48
pGEX-6P-2
28-9546-50
pGEX-6P-3
28-9546-51
205-211
183-88
217-237
244
258
918-935
205-211
183-188
217-237
244
258
918-935
205-211
183-188
217-237
244
258
918-935
205-211
183-188
217-237
244
258
918-935
205-211
183-188
217-237
244
258
205-211
183-188
217-237
244
258
205-211
183-188
217-237
244
258
205-211
183-188
217-237
244
258
―
―
―
930-945
―
―
―
930-966
―
―
―
930-967
―
―
―
930-965
―
921-932
―
―
918-938
―
―
918-938
―
―
918-938
―
945-981
―
945-982
―
945-980
1309-1314
1286-1291
1356
2214
1330-1335
1307-1312
1377
2235
1331-1336
1308-1313
1378
2236
1329-1334
1306-1311
1376
2234
1333-1338
1310-1315
1380
2238
1345-1350
1322-1327
1392
2250
1346-1351
1323-1328
1393
2251
1344-1349
1321-1326
1391
2249
3297
4377
3318
4398
3319
4399
3317
4397
3321
4401
3333
4413
3334
4414
3332
4412
2974
2281-2977
2995
2302-2998
2996
2303-2999
2994
2301-2997
2998
2305-3001
3010
2317-3013
3011
2318-3014
3009
3216-3012
869-891
1020-998
U13850
869-891
1041-1019
U13853
869-891
1042-1020
U13854
869-891
1040-1018
U13855
869-891
1044-1022
U13856
869-891
1056-1034
U78872
869-891
1057-1035
U78873
869-891
1055-1033
U78874
―
―
934-969

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