1 Professor Augusto Pessina Brief CV Present Position Associated

Professor Augusto Pessina Brief CV
Present Position
Associated Professor Professor of Microbiology Faculty of Medicine of the University of Milan.
Head of Cell Culture Laboratory ,Section of Microbiology, Department of Biomedical Surgical
and Dental Sciences.President Gruppo Italiano Staminali Mesenchimali (GISM).Past President:
Associazione Italiana di Colture Cellulari (AICC)
Teaching and research:
Graduated in Biological Science,University of Milan (1971). Specialized in Medical
Microbiology, University of Milan (1978).Assistant Researcher at Centre of Cellular Pathology
of the National Council for Research, Milan (1973-1976).Selected as Italian Participant to the
“Second training course on experimental cancer research of UICC (Amsterdam,1975).Appointed
by the National Board of Education of Italy to the Department of Pathology, Hospital of Desio for
studying the effect of Dioxine (TCDD) on immune system of children accidentally exposed to the
toxic in Seveso (1976-1981).
Professor of General Pathology at School of Pharmacology, Faculty of Pharmacy, University of
Milan.(1982-1985). Appointment by the Board of Education to the Institute of Medical
Microbiology, University of Milan for developing new educational training (1981-1988).
External Examiner for PhD thesis at the School of Biological Sciences, National Cell and Tissue
Culture Centre Dublin City University (Ireland) (1994,2011).Reporter of a PhD thesis at the
Universitè de Bretagne Occidentale (UBO) France(2004).
Management of scientific events
Organizer and promoter of many national and international scientific events as congress, meeting,
workshop and courses.Co-organizer with Prof. Martin Clynes ( Dublin City University-Ireland) of
the "First International Meeting on Drug Resistance in Cancer" (1995).Representative of the
division "Cancer sensitivity and drug resistance" of the European Tissue Culture Society (ETCS)
(1994-1998). Chairman of the Italian Committee of the “3rd World Congress on Alternatives and
Animal use in life science”. Bologna 1999.Member of the Scientific Advisory Board of the National
Institute for Cellular Biotechnology (NICB) of Dublin City University ( Ireland) (2001-2005).CoPresident of the 46PthP ETCS meeting held in Verona ( 2006).President of the Italian Association for
Cell Culture (AICC) ( 2007-2010).Member of the International Scientific Societies and peer
reviewer of may international scientific journals.
Scientific responsible of the following European Commission projects:
“In vitro system for testing haematotoxicity”(1994-1996).“Inhibition of CFU-E/BFU-E by different
classes of chemicals: a comparative study on direct effects on progenitors and the effect on longterm exposure of bone marrow”(1997-1998). “In vitro tests for haematotoxicity: prevalidation and
validation of GM-CFU assay for predicting acute neutropenia”(1997-2001).“Development and
prevalidation of the in vitro assays for the prediction of thrombocytopenia” (Italian Operative Unit
2000-2002)“Purification and regulation of haematopoietic stem cells: in vitro studies”(20012002)."Plasticity of adult stem cells in cord blood" (2002-2003)“Prevalidation of rat CFU-GM
assay”( 2006-2008)
Scientific Unit Responsible inside the projects
“Transplant of adult stem cells in regenerative cellular therapy” of Blood Plan of Regione Lombardia (
2006-2008). “Role of human mesenchymal stem cells in tumor angiogenesis” of AIRC ( Associazione
Italiana per la Ricerca sul Cancro) ( 2010-2012).“LoCMeD - Lab on Chip for testing Myelotoxic effect of
Drugs and chemicals” (Fund from Fondazione Cariplo) (2011-2013). " Mesenchymal stromal cells loaded
with Paclitaxel home glioblastoma vasculature and inhibit tumor growth” of AIRC ( Associazione Italiana
per la Ricerca sul Cancro) ( 2013-2016)
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Some notes on research activity particularly related to hematotoxicity
The experience of Dr Augusto Pessina in the field of hematotoxicity originated in the years 1970 with
studies concerning the relation between the neoplastic growth and the haematopoietic disorders observed in
tumor bearing mice. He developed specific “in vitro” techniques for studying the effect exerted by factors
produced by tumor cells on the proliferation and differentiation of myeloid precursors and in his laboratory
were isolated cytokines active on the haematopoietic progenitors (Int. J. Immunopharmacol. 7,549,1985;
Blut,55,499,1987).
At the first UICC training course organized in Amsterdam on 1975 had as teacher Donald Melcalf
(who is considered the pioneer of the “in vitro” studies on proliferation and differentiation of
haematopoietic progenitors) and the possibility to improve the techniques for assessing the "in vitro" growth
of granulocyte-macrophage committed cells (GM-CFU).
Dr. Pessina has been involved five years (1976-1981)in an international project at the Hospital of
Desio (Milan ,Italy) for studying the haematotoxic effect of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on
the immune system of children accidentally exposed to the toxic in the area of Seveso ( ICMESA accident).
Since 1981 he runs a Laboratory of Cell Culture at the Institute of Microbiology (Milan) and his
researches have been continued on the relationship between tumor growth and the production of cytokines
( CSFs) involved in the proliferation- differentiation of pluripotent stem cells ( CFU-s) and committed
progenitors ( CFU-C) in haematopoiesis ( J. Cancer Res.Clin. Oncol.,102,235,1982; Oncology 39,391,1982;
J.Natl.Cancer Inst.,¸76,1095,1986).
In his laboratory have been established twi new cell lines: a stromal cell line obtained from murine
bone marrow adherent cells (SR-4987) , ( Cytotechnology 8,93,1992 ) available by ATCC (CRL-2028) and
a subclone of WEHI-3B leukemia cells resistant to ciprofloxacin ( Cancer J.,6,291,1993) due to a mutation
of topoisomerase II.
By using SR-4987 cells has been standardized a new "in vitro" method for testing FGFs in biological
samples and developed an “in vitro” model to investigate the role of stromal cells in the modulation of drug
induced hematotoxicity (Life Sci.,65,513,1999).
Researches of the response of different cellular models the biological effects of microbial toxins
demonstrated that the proliferative block exerted by Cholera Toxin may be dependent or not to the cAMP
accumulation and that there are membrane ganglioside with different affinity to CT ad capacity to
transduce the signal. A stable mutant of a leukemia cell line (WEHI-3B) has been established by Dr Pessina
which lacks of the Gal-GalNac-GM1b ganglioside that is a very important functional receptor in WEHI-3b
cells ( Cell .Mol.Biol.,44,933-940,1998. Mol.Cell.Biochem,152,103-112,1995 Mol.Cell Biochem. 233,1926, 2002)
By using GM-CFU techniques Dr Pessina studied the effect of topoisomerase inhibitors, antibiotics
and microbial toxins on different animal cell models ( J.Antimicrob. Chemother., 24,203,1989; Biochim
.Bioph. Acta,1013,206,1989 ; Mol. Cell Biochem.,152,1995).
Since 1994 he managed EC contracts concerning the development of alternative methods for
hematototoxicity testing and coordinated projects of prevalidation and validation of standard operating
procedure based on the use of multipotent stem cells as progenitors of granulocyte-macrophages (GMCFU) , megakaryocytes (Mk-CFU) and erythrocytes (CFU-E).
Based on these results ( Toxicological Sciences , 75,355-367,2003 ) the ECVAM Scientific Advisory
Committee (ESAC) on 2006 unanimously endorsed the CFU-GM assay for predicting acute neutropenia in
humans. Recently a study has been conducted to prevalidate the GM-CFU assay with frozen bone
marrow cells of rat that can contribute to REACH programme ( Altern.To Lab.Animals 37: 417-425,2009.
Altern.To Lab.Animals 38:105-117,2010).
Since 2004 the cell culture laboratory of Dr Pessina is involved in studies on the therapeutic potential of
stem cells and on assays to assess their safety with a particular attention to the microbiological risks
(BBRC, 323:315-322,2004, Crit.Rev.Microbiol, 34:1-12,2008,Transpl Infect Dis .11 : 491-496,2009 ;,Cell
Biol. Int. 34:783-790,2010). More recent his group have indicated that MSCs can be used as drug
carrier. By using a cancer drug ( Paclitaxel) has been demonstrated that human MSCs are able to
uptake and release the drug and that PTX loaded MSCs produced a dramatic inhibition of tumor
growth both in vitro and in vivo. PLoS ONE 6(12): e28321. doi:10.1371/journal.pone.0028321. In recent
paper his laboratori demonstrated that drugs can be released by mesenchymal stem cells into
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microvesicles/exosomes opening new possible biotechnology application for the production of more
bioavailable molecules able to support new therapeutic approaches.
A selection of some publications of the last 15 years 1999-2014.
2014-2010
PASCUCCI L, COCCÈ V, BONOMI A, AMI D, CECCARELLI P, CIUSANI E, VIGANÒ L,
LOCATELLI A, SISTO F, DOGLIA SM, PARATI E, BERNARDO ME, MURACA M,
ALESSANDRI G, BONDIOLOTTI G, PESSINA A. Paclitaxel is incorporated by mesenchymal
stromal cells and released in exosomes that inhibit in vitro tumor growth: a new approach for drug
delivery. Journal Controlled Release 192: 262-270, 2014.
SISTO F, BONOMI A, CAVICCHINI L, COCCÈ V, SCALTRITO MM, BONDIOLOTTI G,
ALESSANDRI G, PARATI E, PESSINA A. Human mesenchymal stromal cells can uptake and
release ciprofloxacin, acquiring in vitro anti-bacterial activity. Cytotherapy. 2014 ,16:181-90.
MARIOTTI M, COLOGNATO R, RIMOLDI M, RIZZETTO M, SISTO F, COCCE V, BONOMI
A, PARATI E, ALESSANDRI G, BAGNATI R, PESSINA A. Mesenchymal Stromal Cells Uptake
And Release Paclitaxel Without Reducing Its Anticancer Activity. ANTI-CANCER AGENTS IN
MEDICINAL
CHEMISTRY,
vol.
13,
p.
523-530,
ISSN:
1871-5206,
doi:
10.2174/1871520611313030015
S. NAVONE, L. PASCUCCI, M. DOSSENA, A. FERRI, G. INVERNICI, F. ACERBI, S.
CRISTINI, G. BEDINI, V. TOSETTI, V. CESERANI, A. BONOMI, A. PESSINA, G. FREDDI, A.
ALESSANDRINO, P. CECCARELLI, R. CAMPANELLA, G. MARFIA, G. ALESSANDRI, E.A.
PARATI (2014). Decellularized silk fibroin scaffold primed with adipose mesenchymal stromal
cells improves wound healing in diabetic mice. STEM CELL RESEARCH & THERAPY, vol. 5, p.
1-15, ISSN: 1757-6512, doi: 10.1186/scrt396
ANTONELLA CONFORTI, SIMONE BIAGINI, NADIA STARC, ALESSANDRA PROIA,AUGUSTO
PESSINA, GIULIO ALESSANDRI, FRANCO LOCATELLI,
MARIA ESTER BERNARDO. Human mesenchymal stromal cells primed with paclitaxel, apart from
displaying anti-tumor activity, maintain their immune regulatory
functions in vitro. Cytotherapy, 2014; 16: 868e870
pen Access
LUIGI BALDUCCI, ANTONELLA BLASI, MARILISA SALDARELLI, ANTONIO SOLETI, AUGUSTO
PESSINA, ARIANNA BONOMI,VALENTINA COCCÈ, MARTA DOSSENA, VALENTINA TOSETTI,
VALENTINA CESERANI, STEFANIA ELENA NAVONE,MARIA LAURA FALCHETTI, EUGENIO
AGOSTINO PARATI AND GIULIO ALESSANDRI. Immortalization of human adipose-derivedstromal
cells: production of cell lines with highgrowth rate, mesenchymal marker expression andcapability to secrete
high levels of angiogenic factors. Stem Cell Research & Therapy 2014, 5:63
M. RASPONI, A. GAZANEO, A. BONOMI, P. OCCHETTA, L. CAVICCHINI, V. COCCE, G. B. FIORE,
A.PESSINA AND A. REDAELLI. Lab-on-Chip for testing Myelotoxic effect of drugs and chemicals
GNB2014, June 25th-27th 2014, Pavia, Italy.
PESSINA A, COCCÈ V, PASCUCCI L, BONOMI A, CAVICCHINI L, SISTO F, FERRARI M,
CIUSANI E, CROVACE A, FALCHETTI ML, ZICARI S, CARUSO A, NAVONE S, MARFIA G,
BENETTI A, CECCARELLI P, PARATI E, ALESSANDRI G. Mesenchymal stromal cells primed
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with Paclitaxel attract and kill leukaemia cells, inhibit angiogenesis and improve survival of
leukaemia-bearing mice. Br J Haematol. 2013; 160: 766-78.
BONOMI A, COCCÈ V, CAVICCHINI L, SISTO F, DOSSENA M, BALZARINI P,
PORTOLANI N, CIUSANI E, PARATI E, ALESSANDRI G, PESSINA A. Adipose-tissuederived stromal cells primed in vitro with paclitaxel acquires anti-tumor activity. Int J
Immunopathol Pharmacol. 2013:26,33-41.
PESSINA A, BONOMI A, CIUSANI E, PASCUCCI L, PARATI E, ALESSANDRI G. Mesenchymal
stromal cells used for carrying and delivering Paclitaxel in “Paclitaxel: Biosynthesis, Mechanisms of Action
and Clinical Uses ”, D. Morales, Nova Science Publishers 2013, pp 141-157.
PESSINA A , BONOMI A, PARATI E, PALLINI R, ALESSANDRI G. Primed mesenchymal
stromal cells for cancer therapy. In “Stem Cell Therapeutics for Cancer”. Ed Khalid Shah, Wiley
Blackwell 2013, pp 189-204.
LAURA DE GIROLAMO, ENRICO LUCARELLI2, GIULIO ALESSANDRI3, MARIA ANTONIETTA
AVANZINI4, MARIA ESTER BERNARDO,ETTORE BIAGI, ANNA TERESA BRINI, GIOVANNA
D’AMICO, FRANCA FAGIOLI, IVANA FERRERO10, FRANCO LOCATELLI,RITA MACCARIO,
MARIO MARAZZI, ORNELLA PAROLINI, AUGUSTO PESSINA AND MARIA LUISA TORRE.
Mesenchymal Stem/Stromal Cells: A New "Cells as Drugs" Paradigm.Efficacy and Critical Aspects in Cell
Therapy. Current Pharmaceutical Design, 2013, 19, 2459-2473
S. DUCHI, P. DAMBRUOSO,E. MARTELLA,G. SOTGIU,A. GUERRINI, E. LUCARELLI,A.
PESSINA,V. COCCÈ,A. BONOMI,AND G. VARCHI.
Thiophene-Based Compounds as Fluorescent Tags to Study Mesenchymal Stem Cell Uptake and Release of
Taxanes BIOCONJUGATE CHEMISTRY, vol. 25, p. 649-655, ISSN: 1043-1802, doi:
10.1021/bc5000498
AUGUSTO PESSINA,VALENTINA COCCÈ, ARIANNA BONOMI, LOREDANA CAVICCHINI,
FRANCESCA SISTO, MAURA FERRARI,EMILIO CIUSANI, STEFANIA NAVONE, GIOVANNI
MARFIA, EUGENIO PARATI AND GIULIO ALESSANDRI. Human Skin-Derived Fibroblasts Acquire In
Vitro Anti-Tumor Potential after Priming with Paclitaxel .Anti-Cancer Agents in Medicinal Chemistry,
2013, 13, 523-30.
PESSINA A.,SISTO F.,COCCE’ V.,CAVICCHINI L.,CIUSANI E.,GRIBALDO L., BONOMI . A
mesenchymal stromal cell line resistant to paclitaxel that spontaneously differentiates into Osteoblast-like
cells. Cell Biol Toxicol 27:169-180,2011.
PESSINA A., BONOMI A, COCCÈ V, INVERNICI G, NAVONE S, CAVICCHINI L et al. . Mesenchymal
Stromal cells primed with paclitaxel provide a new approach for cancer therapy. PLoS ONE 6(12):
e28321.doi:10.1371/journal.pone.0028321 (2011).
PESSINA A.,BONOMI A.,CAVICCHINI L.,ALBELLA B., CERRATO L.,PARENT-MASSIN D.,SIBIRIL
Y.,PARCHMENT R.,BEHRSING H.,VERDERIO P., PIZZAMIGLIO S.,GIANGRECO M., BAGLIO
C.,COCCÈ V.,SISTO F. and GRIBALDO L..Prevalidation of Rat GM-CFU Assay for In Vitro Toxicology
Application. Altern.To Lab.Animals (ATLA) 38:105-117,2010.
PESSINA A., BONOMI A.,SISTO F., BAGLIO C., CAVICCHINI L.,CIUSANI L.,COCCÉ V.,
GRIBALDO L.. CD45+/CD133+ positive cells expanded from umbilical cord blood expressing pdx-1 and
markers of pluripotency. Cell Biol. Int. 34:783-790,2010.
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PESSINA A. Cellule staminali pluripotenti . Le prospettive delle iPSC restano problematiche
Emmeciquadro 38: 27-30,2010.
PESSINA A., BONOMI A. Prediction of potential drug myelotoxicity by in vitro assays
on haematopoietic progenitors. In Methods in Bioengineering: Alternative technologies to
animal testing" eds Tim Maguire and Eric Novik Eds,Artech House Boston-London. Pages
115-131 ,2010 .
LULLI M, DI GESUALDO F.,WITORT E.,PESSINA A.,CAPACCIOLI S.. Cell death:
physiopathological and therapeutic implications. Cell Death Dis. 1: e30; doi: 10.1038/cddis.2010.
2009-2005
PESSINA A.,PARENT-MASSIN D., ALBELLA B.,VAN DEN HEUVEL R.,CASATI
S.,CROERA C.,MALERBA I.,SIBIRIL Y.,GOMEZ.S., DE SMEDT.,GRIBALDO L..
Application of human CFU-Mk assay to predict potential thrombocytotoxicity of drugs.
Toxicology in Vitro, 23:194-200,2009.
PESSINA A., BONOMI A., BAGLIO C., CAVICCHINI L., GRIBALDO Refinement and
Optimisation of the rat CFU-GM assay to use cryopreserved bone marrow cells for “in vitro” toxicology
application . Altern.To Lab.Animals (ATLA) 37: 417-425,2009.
PESSINA A.,BONOMI A.,COCCÈ V., BERNARDO M.E., COMETA A.M., FERRARI M., SISTO
F.,CAVICCHINI L.,LOCATELLI F. Assessment of human herpesvirus-6 infection in mesenchymal stromal
cells ex vivo expanded for clinical use.Transpl Infect DisT.11 : 491-496,2009 .
PESSINA A. BONOMI A., BAGLIO C.,CAVICCHINI L.,SISTO F., NERI MG.,GRIBALDO
L..Microbiological Risk Assessment In Stem Cell Manipulation. Crit.Rev.Microbiol, 34:1-12,2008
PESSINA A., BONOMI A.,CASATI S.,COLLOTTA A.,CROERA C.,MARAFANTE
E.,PALITTI F.,GRIBALDO L.. Mitochondrial function, apoptosis and cell-cycle delay in the WEHI-3B
leukemia cell line and its variant ciprofloxacin-resistant WEHI-3B/CPX .
Cell Prolif.,40,568-579,2007.
PESSINA A., BONOMI A. CFU-GM assay for evaluation of drug Myelotoxic Activity
Curr.Prot.Toxicol. 34:20.2.1-20.2.18, Eds. John Wiley & Sons,Inc., 2007.
PESSINA A., TURLIZZI E., BONOMI A., GUIZZARDI F., CAVICCHINI L.,CROERA C., BAREGGI
S..In vitro toxicity of clozapine,olanzapine and quetiapine on granulocyte-macrophage progenitors(GMCFU). Pharmacopsychiatry,39,20-22,2006.
PESSINA A., CROERA C.,SAVALLI N.,BONOMI A.,CAVICCHINI L.P1P,TURLIZZI E., GUIZZARDI
F.,GUIDO L.,DAPRAI L.,P1P NERI MG..Bcl-2 down modulation in WEHI-3B/CTRES cells resistant to
Cholera Toxin (CT)-induced apoptosis. Cell Res. 16:306-312,2006
PESSINA A., and GRIBALDO L..The key role of adult stem cells: therapeutic perspectives. Curr. Med.
Res. Opin.,22,2287-2300,2006
PESSINA A., MALERBA I., GRIBALDO L. Haematotoxicity testing by cell clonogenic assay in drug
development and preclinical trials Curr.Pharmaceut.Design 11,1055-1065,2005.
1999-2004
PESSINA A., CROERA C., BAYO M., MALERBA I., PASSARDI L., CAVICCHINI L.,
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NERI M.G., GRIBALDO L. A methylcellulose microculture assay for the in vitro assessment of drug
toxicity on granulocyte- macrophage progenitors (CFU-GM). ATLA 32:17-23, 2004
PESSINA A.,ELETTI B.,CROERA C.,SAVALLI N.,DIODOVICH C.,GRIBALDO L..
Pancreas developing markers expressed on human mononucleated umbilical cord blood cells.Biochem
Biophys. Res. Comm. 323:315-322,2004
PESSINA A., ALBELLA B. , BAYO M., BUEREN J.,.BRANTOM P., CASATI S.,.
CROERA C.,GAGLIARDI G FOTI P, PARCHMENT R., PARENT-MASSIN D.,
SCHOETERS G., SIBIRIL Y.,VAN DEN HEUVEL R.,.GRIBALDO L.
Application of the CFU-GM assay to predict acute drug-induced neutropenia:an international blind trial to
validate a prediction model for the maximum tolerated dose(MTD) of myelosuppressive xenobiotics.
Toxicological Sciences , 75,355-367,2003 .
ROSEEUW E.,COESSENS V.,BALAZUC AM.,LAGRANDERIE M.,CHAVAROT P.,
PESSINA A., NERI MG., SCHACHT E.,MARCHAL G., DOMURADO D..
Synthesis, degradation and antimicrobial properties of targeted macromolecular prodrugs of norfloxacin.
Antimicrob. Agents Chemother.,47,3435-3441,2003.
PESSINA A., BAYO M.,CROERA C.,MERINGOLO F.,NERI MG.MONTESISSA L.,
RAIMONDI A. In vitro sensitivity of Human Gastric Cancer Cells( HGC-27) to Helicobacter pylori
cytotoxin. Scand. J. Gastroenterol. 12:1228-1234,2003.
PESSINA A., GIULIANI A., CROERA C., FOTI P., MASCOLO L. GAGLIARDI G. NERI MG. Selection
of a WEHI-3B leukemia cell subclone resistant to inhibition by cholera toxin. Mol.Cell Biochem., 233,1926,2002.
PESSINA A., GAGLIARDI G.,CROERA C.FOTI P.,DASSI C.BRAMBILLA P.,NERI MG..Secretion of
basic fibroblast growth factor (FGF-2) by WEHI-3B myelomonocitic leukemia cells. Growth Factors ,
20,121-129,2002.
PESSINA A.,RAIMONDI A:;CROERA C:, ACCHINI M:,MINEO E.,FOTI P:,NERI MG:
Altered DNA
cleavage activity of topoisomerase II from WEHI-3B leukemia cells resistant to ciprofloxacin. Anti-Cancer
Drugs,12, 441-451,2001
PESSINA A,RAIMONDI A.CERRI A. PICCIRILLO M, NERI MG, CROERA C. ,FOTI P.,BERTI E.
High sensitivity of human epidermal keratinocytes (HaCaT) to topoisomerase inhibitors. Cell
Proliferation ,34,243-252,2001.
PESSINA A., ALBELLA B. , BUEREN J.,.BRANTOM P., CASATI S.,.CROERA C., GAGLIARDI G.,
GRIBALDO L, FOTI P., PARCHMENT R., PARENT-MASSIN D., SCHOETERS G., SIBIRIL Y.,VAN
DEN HEUVEL Prevalidation of a model for predicting acute neutropenia by Colony Forming UnitGranulocyte/Macrophage CFU-GM) assay Toxicology in Vitro, 15,729-740,2001.
GRIBALDO L, MALERBA I.,COLLOTTA A.,CASTI S.,PESSINA A.. Inhibition of CFGU-e/BFU-E by
3’-azido- 3’-deoxythymidine,chlorpropamide and protoporphirin IX zinc (II): a comparison between direct
exposure of progenitor cells and long-term exposure of bone marrow. Toxicological Sciences 58, 96101,2000.
PESSINA A.,PICCIRILLO M.,MINEO E.,CATALANI P.,GRIBALDO L., MARAFANTE E., NERI
MG.,RAIMONDI A. Role of SR-4987 stromal cells in the modulation of Doxorubicin toxicity to in vitro
granulocyte-macrophage progenitors (GM-CFU). Life Sciences, 65,513-523,1999.
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PESSINA A., ROSSONI L.,PICCIRILLO M.,SALA G.,NERI MG.. Preliminary study on in vitro activity
and cytotoxicity on cell cultures of a new polyene antifungal molecule (SPA-S-843). Eur. J. Pharmac.
Biopharmac. 47,179-184,1999.
GRIBALDO L.,CASATI S.,CASTOLDI A., PESSINA A.Comparison of In Vitro drug sensitivity
of Human Granulocyte-Macrophage progenitors from two different origins:umbilical cord blood
and bone marrow. Exp. Hematol., 27,1593-1598,1999.
FULL PAPERS
BIGI G.,PESSINA A- Immunità e tumori. Sapere ,753,24-29,1972..
CLERICI E.,GAROTTA G.,PORTA C.,PESSINA A.,VILLA ML. Immunological aspects of amyloydosis.
Isr.J.Med.Sc., 9,881-887,1973.
PORTA C.,PESSINA .A. Complemento e infiammazione. Sapere, 757,28-30,1973.
PESSINA A.,BRAMBILLA P.,BALZARIN D. Inhibition of Ehrlich ascites carcinoma (EAC) growth by carbonyl iron.
Experientia,34,1518-1519,1978.
PESSINA A.,BRAMBILLA P.,MOCARELLI P. Surface antigen on Ehrlich Ascites Tumor Cells. Biomedicine.,33,
105-109,1980.
PESSINA A .,ERIDANI S., BRAMBILLA P., CATTORETTI G, MAROCCHI A., MOCARELLI P. Effect of tumor
related factors on the in vivo and in vitro colony forming ability of normal mouse marrow cells. Neoplasma,28,541548,1981
PESSINA A.,BRAMBILLA P.,VILLA S.,MAROCCHI A.,MOCARELLI P.. Factors produced by macrophages and
tumor cells: influence on the granulocytic precursor cells(CFU-C) in normal and tumor bearing mice. J.Cancer
Res.Clin.Oncol.,102,235-244,1982.
PESSINA A.,BRAMBILLA P.,VILLA S.,MOCARELLI P.. CFU-s and CFU-C proliferation after treatment of normal
bone marrow cells with tumoral ascitic fluid. Oncology, 39,391-395,1982.
PESSINA A.,NERI M.G.,MUSCHIATO A..Effect of heat-labile enterotoxin (LT) produced by Escherichia coli on in
vitro cell proliferation. Experientia.,38,1056-1057,1982.
SIRCHIA G.,ROSSO DI SAN SECONDO V.E.M.,TEDESCO F.,FORTIS C.,ALCINI D.,GIOVANETTI
A.M.,GUAZZOTTI L.,ZANUSSI C.,SCORZA R.,FABIO G.,BONARA P.,MERONI P.L.,SABBADINI
M.G.,VANOLI M., PETTENATI C.,MOCARELLI P.,PESSINA A., BRAMBILLA P.,MAROCCHI A., CAREDDU
P.,BARDARE M.,CORONA F.,CHIAPPINO G.,NAVA C.,MEREGALLI G.,GARATTINI S., SPREAFICO
F.,VECCHI A.,MANTOVANI A.,MARUBINI E.,MILANI S..
Exposure to TCDD:Immunologic Effects. In:Plans
for clinical and epidemiologic follow-up
after area-wide chemical contamination.
Proc.Int.Workshop,Washington,D.C.,March
1980.Eds.L. Dardanoni, R.W.Miller. Natl.Acad.Press,Washington M
arch 1982. Pagg.234-264.
PESSINA A.,NERI M.G.,MUSCHIATO A.,RAIMONDI A.. Inhibition of granulocytic-macrophagic precursor cells
(CFU-C) by heat-labile enterotoxin (LT) produced by Escherichia coli. Biomed. Pharmacother.37,447-452,1983.
PESSINA A.,NERI M.G.. Factors affecting the proliferation of a murine T-cell lymphoma. Chemioterapia,S5,459460,1983.
PESSINA A.,BRAMBILLA P.,MOCARELLI P..Modulation of EL-4 mouse lymphoma cell proliferation by
macrophages and tumor related factors. Blut 49,45-51,1984.
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SEZZANO P.,RAIMONDI A.,PESSINA A.,NERI M.G..Isolation and partial chracterization of temperate
bacteriophages from enteropathogenic strains of Escherichia coli O111:K58. Acta Virologica,29,73-78,1985.
PESSINA A.,BRAMBILLA P.,NERI M.G.,MOCARELLI P..Production of colony stimulating factor by EL-4
lymphoma cells stimulated with phytohemoagglutinin. Int. J. Immunopharmac., 7,549-553,1985.
RAIMONDI S.,DONGHI R.,MONTAGUTI A.,PESSINA A.,DEHO' G..Analysis of spontaneous deletion mutants of
satellite bacteriophage P4. J.Virol.,54,233-235,1985.
PESSINA A.,NERI M.G..Colony stimulating factor produced by T-lymphoma cells. Chemioterapia,S2,11721173,1985.
PESSINA A.,NERI M.G.,MUSCHIATO A.,BRAMBILLA P.,MAROCCHI A. MOCARELLI P.. Colony stimulating
factor produced by murine adrenocortical tumor cells. J.Natl.Cancer Inst.,76,1095-1099,1986.
PESSINA A.,MUSCHIATO A.,COLOMBO M.,NERI M.G..Escherichia coli heat-labile enterotoxin:biological activity
of crude filtrate and fractions isolated by gel chromatography. Chemioterapia, S2,46-48,1987.
NERI M.G.,OTTOLENGHI A.,PESSINA A.,DE CHIARA A., MAGLIANO E..Detection of enterotoxigenic strains of
E.coli in children with acute diarrhea. Chemioterapia,S2,44-45,1987.
PESSINA A.,MUSCHIATO A.,NERI M.G..Isolation of a colony stimulating factor produced by L1210 murine
leukemia cells. Blut.,55,499-504,1987.
PESSINA A.,MATTINA R.,COCUZZA C.E.. Attività biologica dei chinoloni sulle cellule procariote ed eucariote.
Farmaci & Terapia,4,185-190,1987.
NERI M.G.,PESSINA A.,OTTOLENGHI A.,COCUZZA G.. Enterobatteri produttori di tossina termolabile nelle
diarree acute. L'Igiene Moderna,89,602-611,1988.
PESSINA A.,NERI M.G.,IANNETTA M.,MINEO E.. Effect of fluorinated quinolones on heat-labile toxin production
by Escherichia coli. J.Chemother.,S4,138-139,1989.
PESSINA A.,NERI M.G.,MINEO E.,COCUZZA C.E..Effect of quinolones on the production of colony stimulating
factors by macrophages. J.Chemother.S4,419-420,1989.
PESSINA A.,NERI M.G.,MUSCHIATO A.,MINEO E.,COCUZZA G..Effect of fluoroquinolones on the in vitro
proliferation of myeloid precursor cells. J.Antimicrob. Chemother.,24,203-208,1989.
PESSINA A.. Citochine: quale futuro in campo diagnostico? Diagnosis,1,113-116,1989.
PESSINA A.,MINEO E.,MASSERINI M.,NERI M.G.,COCUZZA C.E.. Inhibition of murine leukemia (WEHI-3B and
L1210) proliferation by cholera toxin B subunit. Biochim. Bioph. Acta.1013,206-211,1989.
PESSINA A.,NERI MG.,GRIBALDO L.,MINEO E.,COCUZZA G.. Sensibilità in vitro di cellule leucemiche e
precursori mieloidi murini a fluorochinoloni. J.Chemother.,S2,583-585,1990.
PESSINA A.,MINEO E.,NERI M.G.,COCUZZA G..In vitro effect of fluoroquinolones on macrophages response to
LPS stimulation and phagocytosis. J.Chemother.,S4,542-544,1991.
PESSINA A.,MINEO E.,GRIBALDO L.,MANCINA G.,NERI MG.. Lack of in vitro antiviral activity of
fluoroquinolones against herpes simplex virus type 2. Arch.Virol.,122,263-269,1992.
PESSINA A., MINEO E.,NERI MG.,GRIBALDO L.,COLOMBO R., BRAMBILLA P.,ZALESKIS G.. Establishment
and characterization of a new murine cell line (SR-4987) derived from marrow stromal cells. Cytotechnology.,8,93102,1992.
PESSINA A. Dimmi cosa fai e ti dirò chi sei. Editoriale Diagnosis,5,201,1992.
8
PESSINA A.,GRIBALDO L.,MINEO E.,NERI MG.. Effect of fluoroquinolones on the in vitro production of tumor
necrosis factor(TNF) by P388D1 cells. J.Chemother.,5,562-564,1993
PESSINA A.,MINEO E.,GRIBALDO L.,MANCINA G.,NERI MG.,MORAN E.,CLEARY I., CLYNES M.
Susceptibility of leukemia cell lines to quinolones and induction of resistance to ciprofloxacin in WEHI-3B(D+)
leukemia cells. Cancer J.,6,291-297,1993.
PESSINA A. Topoisomerase I in multiple drug resistance. Cytotechnology, 12,127-135,1993.
PESSINA A.,GRIBALDO L.,MINEO E.,NERI MG.. In vitro short-term and long-term cytotoxicity of fluoroquinolones
on murine cell lines. Ind. J. Exp. Biol.,32,113-118,1994.
RAIMONDI A.,PESSINA A. , COCUZZA G. Outer membrane permeability of Serratia marcescens to meropenem and
imipenem. J.Chemother.6,363-367,1995.
PESSINA A.,RAIMONDI A. , NERI MG. Different biological conditions influencing bacterial adherence assay.
J.Chemother.,7,8-11,1995
PESSINA A.,ZALESKIS G.,MINEO E.,CERRI A., NERI MG. Expression of CD antigen on SR-4987 murine marrow
stromal cells. J.Exp. Clin. Cancer Res. 14,197-198,1995.
NERI M.G.,MINEO E.,PICCIRILLO M., PESSINA A.. Attività microbicida di benzalconio cloruro-alcansolfonato
sodico su microorganismi di interesse veterinario. Ingegneria Alimentare, 6,25-29,1995.
GIULIANI A.,CALAPPI E., MINEO E.,NERI MG.,MASSERINI M., GALLINA A.,PESSINA A. The different
inhibiting effect of cholera toxin on two leukemia cell lines does not correlate with their toxin binding capacity.
Mol.Cell.Biochem,152,103-112,1995
PESSINA A. Coltivatori di cellule in vitro. Kos,119/120,23-25,1995.
PESSINA A.,PICCIRILLO M.,MINEO E.,TASSI E.,BRAMBILLA P., NERI MG.. A simple quantitative bioassay for
basic fibroblast growth factor using SR-4987 cells as target. Meth. Mol. Cell. Biol.,5,230-236,1995.
RAIMONDI A .,PESSINA A., NERI MG.. Loss of Porins Following Carbapenem-Resistance Selection and Adherence
Modification in Enterobacteria. J.Chemother. 7,171-174,1995.
GRIBALDO L.,BUEREN J.,DELDAR A.,HOKLAND P.,MEREDITH C.,MONETA D., MOSESSO P.,
PARCHMENT R., PARENT-MASSIN D.,PESSINA A., SAN ROMAN J., SCHOETERS G.. The use of in vitro
systems for evaluating haematotoxicity. ATLA , 24,211-231,1996
PESSINA A.,NERI MG.,MINEO E.,PICCIRILLO M.,GRIBALDO L.,BRAMBILLA P.,ZALESKIS G.,UJHAZY P..
Expression of B cell markers on SR-4987 cells derived from murine bone marrow stroma. Exp. Hematol. 25,536541,1997.
GRIBALDO L.,PICCIRILLO M.,CASATI S.,COLLOTTA A.,MINEO E.,PESSINA A .. Drug sensitivity of
granulocyte-macrophage precursors (GM-CFU) from fresh murine bone marrow and from long- term bone marrow
cultures. Toxicology In Vitro, 12, 39-45, 1998.
PESSINA A.,MINEO E.,NERI MG.,PICCIRILLO M.,VALORE L.,GIULIANI A..SR-4987 and L1210 cell lines :two
models in which the cholera toxin susceptibility does not correlate with cAMP accumulationa and ganglioside content.
Cell .Mol.Biol.,44,933-940,1998.
PARCHMENT R.E , PESSINA A. Topoisomerase I inhibitors and drug resistance. Cytotechnology, 27, 149164,1998.
PESSINA A.,PICCIRILLO M.,MINEO E.,CATALANI P.,GRIBALçDO L.,MARAFANTE E., NERI
MG.,RAIMONDI A. Role of SR-4987 stromal cells in the modulation of Doxorubicin toxicity to in vitro granulocytemacrophage progenitors (GM-CFU). Life Sciences, 65,513-523,1999.
PESSINA A. Appunti di Viaggio. Un ricercatore di fronte ai dati della realtà. Emmeciquadro,10,25-30,1999.
9
PESSINA A., ROSSONI L.,PICCIRILLO M.,SALA G.,NERI MG.. Preliminary study on in vitro activity and
cytotoxicity on cell cultures of a new polyene antifungal molecule (SPA-S-843). Eur. J. Pharmac. Biopharmac. 47,179184,1999.
GRIBALDO L.,CASATI S.,CASTOLDI A., PESSINA A.Comparison of In Vitro drug sensitivity of Human
Granulocyte-Macrophage progenitors from two different origins:umbilical cord blood and bone marrow. Exp.
Hematol., 27,1593-1598,1999.
GRIBALDO L, MALERBA I.,COLLOTTA A.,CASTI S.,PESSINA A.. Inhibition of CFGU-e/BFU-E by 3’-azido- 3’deoxythymidine,chlorpropamide and protoporphirin IX zinc (II): a comparison between direct exposure of progenitor
cells and long-term exposure of bone marrow. Toxicological Sciences 58, 96-101,2000.
PESSINA A.,RAIMONDI A:;CROERA C:, ACCHINI M:,MINEO E.,FOTI P:,NERI MG:: Altered DNA cleavage
activity of topoisomerase II from WEHI-3B leukemia cells resistantto ciprofloxacin. Anti-Cancer Drugs,12, 441451,2001
PESSINA A,RAIMONDI A.CERRI A. PICCIRILLO M, NERI MG, CROERA C. ,FOTI
sensitivity of human epidermal keratinocytes (HaCaT) to
topoisomerase inhibitors. Cell Proliferation ,34,243-252,2001.
P.,BERTI E. High
PESSINA A., ALBELLA B. , BUEREN J.,.BRANTOM P., CASATI S.,.CROERA C.,
GAGLIARDI G.,
GRIBALDO L, FOTI P., PARCHMENT R., PARENT-MASSIN D.,
SCHOETERS G., SIBIRIL Y.,VAN DEN
HEUVEL Prevalidation of a model for
predicting acute neutropenia by Colony Forming Unit-Granulocyte/Macrophage CFU-GM) assay Toxicology in Vitro,
15,729-740,2001.
PESSINA A., GIULIANI A., CROERA C., FOTI P., MASCOLO L. GAGLIARDI G. NERI MG. Selection of a
WEHI-3B leukemia cell subclone resistant to inhibition by cholera toxin. Mol.Cell Biochem., 233,19-26,2002.
PESSINA A., GAGLIARDI G.,CROERA C.FOTI P.,DASSI C.BRAMBILLA P.,NERI MG.. Secretion of basic
fibroblast growth factor (FGF-2) by WEHI-3B myelomonocitic leukemia cells. Growth Factors , 20,121-129,2002.
PESSINA A., ALBELLA B. , BAYO M., BUEREN J.,.BRANTOM P., CASATI S., CROERA C., PARCHMENT
R., PARENT-MASSIN D., SCHOETERS G., SIBIRIL Y.,VAN DEN HEUVEL R.,.GRIBALDO L. In vitro tests for
haematotoxicity: Prediction of drug induced Myelosuppression by the CFU-GM assay. ATLA (Altern. Lab. Anim) 30
S2:75-79, 2002.
RAIMONDI A.,SISTO F.,RIZZARDI R., PESSINA A.,TICOZZI R.,NERI MG.. Sequenza di due forme alleliche del
gene di beta-lattamasi cromosomiale di S.marcescens. Effetto della loro espressione sulla sensibilità ad antibioticibetalattamici di ospiti Trasformati GIMNOC,Vol VI:53-56,2002.
PESSINA A., ALBELLA B. , BAYO M., BUEREN J.,.BRANTOM P., CASATI S.,.CROERA C.,GAGLIARDI G
FOTI P, PARCHMENT R., PARENT-MASSIN D.,SCHOETERS G., SIBIRIL Y.,VAN DEN HEUVEL
R.,.GRIBALDO L.Application of the CFU-GM assay to predict acute drug-induced neutropenia:an international blind
trial to validate a prediction model for the maximum tolerated dose(MTD) of myelosuppressive xenobiotics.
Toxicological Sciences , 75,355-367,2003 .
ROSEEUW E.,COESSENS V.,BALAZUC AM.,LAGRANDERIE M.,CHAVAROT P.,PESSINA A., NERI MG.,
SCHACHT E.,MARCHAL G., DOMURADO D..Synthesis, degradation and antimicrobial properties of targeted
macromolecular prodrugs ofnorfloxacin. Antimicrob. Agents Chemother.,47,3435-3441,2003.
PESSINA A., BAYO M.,CROERA C.,MERINGOLO F.,NERI MG.MONTESISSA L.,RAIMONDI A. In vitro
sensitivity of Human Gastric Cancer Cells( HGC-27) to Helicobacter pylori cytotoxin. Scand. J. Gastroenterol.
12:1228-1234,2003.
PESSINA A. Le cellule staminali. Ricerca, problemi applicazioni. Emmeciquadro 17:16-24,2003.
PESSINA A.,GIULIANI A.,CROERA C.,GAGLIARDI G.,NERI. MG. Different response to "in vitro" cholera toxin
treatment by three murine cell models. L'Igiene Moderna 120:305-321,2003.
10
PESSINA A., CROERA C., BAYO M., MALERBA I., PASSARDI L., CAVICCHINI L., NERI M.G., GRIBALDO L.
A methylcellulose microculture assay for the in vitro assessment of drug toxicity on granulocyte- macrophage
progenitors (CFU-GM).
ATLA 32:17-23, 2004
PESSINA A.,ELETTI B:,CROERA C.,SAVALLI N.,DIODOVICH C.,GRIBALDO L..Pancreas developing markers
expressed on human mononucleated umbilical cord blood cells.Biochem Biophys. Res. Comm. 323:315-322,2004
PESSINA A., MALERBA I., GRIBALDO L. Haematotoxicity testing by cell clonogenic assay in drug development
and preclinical trials Curr.Pharmaceut.Design 11:1055-1065,2005.
PESSINA A.,TURLIZZI E.,BONOMI A. et al. In vitro toxicity of clozapine,olanzapine and quetiapine on granulocytemacrophage progenitors(GM-CFU).Pharmacopsychiatry,39,20-22,2006.
PESSINA A.,CROERA C.,SAVALLI N. et al. Bcl-2 down modulation in WEHI-3B/CTRES cells resistant to Cholera
Toxin (CT)-induced apoptosis. Cell Res. 16:306-312,2006
PESSINA A. and GRIBALDO L. The key role of adult stem cells: therapeutic perspectives.
Opin.,22,2287-2300,2006.
Curr. Med. Res.
PESSINA A.,BONOMI A.CASATI S. et al. Mitochondrial function, apoptosis and cell-cycle delay in the WEHI-3B
leukemia cell line and its variant ciprofloxacin-resistant WEHI-3B/CPX. Cell Prolif.,40,568-579,2007.
PESSINA A.,BONOMI A.,BAGLIO C. et al. Microbiological Risk Assessment In Stem Cell Manipulation.
Crit.Rev.Microbiol, 34:1-12,2008.
PESSINA A.,PARENT-MASSIN D., ALBELLA B.et al. Application of human CFU-Mk assay to predict potential
thrombocytotoxicity of drugs. Toxicology in Vitro, 23:194-200,2009.
PESSINA A.,BONOMI A.,BAGLIO C.,CAVICCHINI L.,GRIBALDO Refinement and optimisation of the rat CFUGM assay to use cryopreserved bone marrow cells for “in vitro” toxicology application. Altern.To Lab.Animals
(ATLA) 37: 417-425,2009.
PESSINA A.,BONOMI A.,COCCÈ V. et al. Assessment of human herpesvirus-6 infection in
cells ex vivo expanded for clinical use.Transpl Infect Dis T.11 : 491-496,2009.
mesenchymal stromal
PESSINA A.,BONOMI A.,CAVICCHINI L. et al. Prevalidation of Rat GM-CFU Assay for In Vitro Toxicology
Application. Altern.To Lab.Animals (ATLA) 38:105-117,2010.
PESSINA A., BONOMI A.,SISTO F. er al. CD45+/CD133+ positive cells expanded from umbilical cord blood
expressing pdx-1 and markers of pluripotency. Cell Biol. Int.34:783-790,2010.
PESSINA A. Cellule staminali pluripotenti. Le prospettive delle iPSC restano problematiche. Emmeciquadro 38: 2730,2010.
LULLI M, DI GESUALDO F.,WITORT E.,PESSINA A.,CAPACCIOLI S. Cell death: physiopathological and
therapeutic implications. Cell Death Dis. 1: e30; doi:10.1038/cddis.2010.8
PESSINA A.,SISTO F.,COCCE’ V. et al. A mesenchymal stromal cell line resistant to paclitaxel that spontaneously
differentiates into Osteoblast-like cells. Cell Biol Toxicol 27:169-180,2011.
PESSINA A., BONOMI A., COCCE’ V. et al. Mesenchimal stromal cells primed with paclitaxel provide a new
approach for cancer therapy. PloSONE, 6, Issue 12 , e28321,2011.
PESSINA A , COCCÈ V,.BONOMI A.,CAVICCHINI L., SISTO F., FERRARI M., NAVONE S., MARFIA G.,
PARATI E., ALESSANDRI G. Human Skin-Derived Fibroblasts Acquire In Vitro Anti-Tumor Potential after Priming
with Paclitaxel. Anti-Cancer Agents in Medicinal Chemistry, 2013, 13, 523-30.
11
PESSINA A , COCCÈ V,. PASCUCCI L.,BONOMI A.,CAVICCHINI L., SISTO F., FERRARI M.,CIUSANI
E.,NAVONE S., MARFIA G., BENETTI A. CECCARELLI P.,PARATI E., ALESSANDRI G. Mesenchymal stromal
cells primed with Paclitaxel attract and kill leukemia cells, inhibit angiogenesis and improve survival of leukemiabearing mice. British Journal of Haematology, 2013, 160, 766–778
BONOMI A., COCCÈ V,.CAVICCHINI L., SISTO F., DOSSENA M.,BALZARINI P. PORTOLIANI N.,CIUSANI
E.,PARATI E., ALESSANDRI G. PESSINA A. Adipose-tissue-derived stromal cells primed in vitro with paclitaxel
acquires anti-tumor activity. Int. J.Immunopath. Pharmacol. 2013,26 ,33-42.
L.DE GIROLAMO, E.LUCARELLI, G. ALESSANDRI, M.A. AVANZINI, M.E. BERNARDO , E.BIAGI, A.T.BRINI,
G.D’AMICO, F.FAGIOLI, I.FERRERO, F.LOCATELLI, R.MACCARIO, M.MARAZZI, O.PAROLINI, A.
PESSINA* ,M.L.TORRE * Italian Mesenchymal Stem Cell Group (GISM). Mesenchymal Stem/Stromal Cells: A New
"Cells as Drugs" Paradigm. Efficacy and Critical Aspects in Cell Therapy.Curr. Pharmaceut. Des. 2013,19,2459-73.
CHAPTERS OF BOOKS
CLERICI E.,GAROTTA G.,PORTA C.,BIGI G.,PESSINA A. Reattività immunologica nel feto e nel neonato. In:
Corso di perfezionamento in metodiche immunologiche e Simposio sui problemi di immunologia di interesse
pediatrico. Ed. Piccin,Padova. Pagg 263-286,1977.
CLERICI E.,PORTA C.,BIGI G.,GAROTTA G.,PESSINA A. Tecniche per la quantizzazione delle cellule staminali
dei B e T linfociti e metodi di studio dell'anticorpopoiesi "in vitro".In: Corso di perfezionamento in metodiche
immunologiche e Simposio sui problemi di immunologia di interesse pediatrico. Ed. Piccin,Padova. Pagg. 6683,1977.
PESSINA A. I colony stimulating factors: regolatori della proliferazione e della differenziazione dei precursori
granulo- macrofagico. Dispensa per la "Scuola di Specializzazione in Farmacologia ",Facolta’ di Farmacia,1983.
PESSINA A.,MUSCHIATO A.,NERI M.G.. Isolation of colony stimulating factors produced in vitro by murine adrenal
tumor cells. In : Biological Regulation of Cell Proliferation . Baserga R.,Foa P.,Metcalf D.,Polli E.E.,Eds. Raven
Press,New York,1986. Vol. 34,pagg 191-194.
PESSINA A.. The Granulocyte/Macrophage Colony Forming Unit Assy. In:Animal Cell Culture Techniques. Ed.M.
Clynes ,pp 217-229,Springer-Verlag,, Heidelberg 1998
PESSINA A., ALBELLA B. , BUEREN J.,.BRANTOM P., CASATI S., CORRAO G., GRIBALDO L.,
PARCHMENT R.,PARENT-MASSIN D.,PICCIRILLO M.,RIO B.,SACCHI S.,SCHOETERS G.,Van Den HEUVEL
R.. Method developement for a prevalidation study of in vitro GM-CFU assay for predicting myelotoxicity.. In:
Progress in the reduction, refinement and replacement of animal experimentation. Balls M,van Zeller A-M, Halder M
(eds). Amsterdam: Elsevier, 2000 Pages 679-691.
PESSINA A.,BONOMI A. CFU-GM assay for evaluation of drug Myelotoxic Activity
20.2.18, Eds. John Wiley & Sons,Inc., 2007.
Curr.Prot.Toxicol. 34:20.2.1-
PESSINA A.,BONOMI A. Prediction of potential drug myelotoxicity by in vitro assays on haematopoietic
progenitors . In “Methods in Bioengineering “ Tim Maguire and Eric Novik Eds, Artech House Boston-London.
Pages 115-131 ,2010 .
PESSINA A , .BONOMI A., PARATI E., PALLINI R., ALESSANDRI G. Primed mesenchymal stromal cells for
cancer therapy. In Stem Cell Therapeutics for Cancer . Ed Khalid Shah. John Wiley & Sons, Inc., USA.( 2013 , In
Press)
PESSINA A., BONOMI A., CIUSANI E., PASCUCCI L.,PARATI E., ALESSANDRI G.
“Paclitaxel: Biosynthesis, Mechanisms of Action and Clinical Uses''in “ Mesenchymal stromal cells used for
carrying and delivering Paclitaxel” NOVA SCIENCE PUBLISHERS,INC.Hauppauge, NY 11788-3619, USA ( 2013 in press)
ACTA, PROCEEDINGS AND EXTENDED ABSTRACTS
12
MOCARELLI P.,VILLA ML.,PESSINA A.,BIGI G.,CLERICI E. Meccanismi cellulari della immunodepressione da
tumore.Rendiconti Acc. Naz. Lincei ,XLIX,446-451,1970.
PESSINA A.,PORTA C.Lymphoid repopulation and immune response in X-irradiated mice. Atti Simposio
Internaz.Ematologia. Ed.Viscontea, Milano.pagg. 5-12 ,1975.
ERIDANI S.,PESSINA A.,BRAMBILLA P.,PERRONE G.,MOCARELLI P. Influenza di fattori di origine neoplastica
sulla ripopolazione esogena di CFU-s nel topo. In: Lo studio delle cellule staminali in vitro. Ed.M.A.Brunelli et
al.,Esculapio,Bologna. Pag.267-271,1976.
SEZZANO P.,RAIMONDI A.,GHIDONI G.,PESSINA A.,NERI MG..Caratteristiche di batteriofagi temperati isolati da
E.coli enteropatogeni di differenti sierogruppi. Atti XX°ongr.Naz.Microbiologia..Brescia-Gardone, Maggio 1983.
Pagg.542-543.
SEZZANO P.,RAIMONDI A.,PESSINA A.,NERI MG.. Fattori di patogenicita e lisogenia in E.coli O111:K58.Atti
XX°Congr.Naz. Microbiologia..Brescia-Gardone, Maggio 1983.Pagg.307-308.
RAIMONDI A.,SEZZANO P.,PESSINA A.,DONGHI R.,DEHO' G.. Isolamento e caratterizzazione di mutanti
delezione del batteriofago satellite P4. Atti XX° Congr.Naz.Microbiologia..Brescia-Gardone, Maggio 1983.
Pagg.234-235.
PESSINA A.,BRAMBILLA P.,MOCARELLI P.,NERI MG.. Conditioned media from tumoral and transformed cell
cultures:biological acttivity on stem cell differentiation. Proc.13th Int.Congress Chemother.,K.H.Spitzy and K.Karrer
Eds.,,Vienna Settembre1983.
PESSINA A.,MUSCHIATO A.,NERI M.G.. Production of colony stimulating factors by L1210 leukemia cells. In:"In
vitro control of cell differentiation",Ed.Rizzoli C.,Bologna,1985. Pag.18.
PESSINA A.,MUSCHIATO A.,NERI M.G.. Modulazione dell'attività di colony stimulating factors da parte di tossina
termolabile di E.coli. Atti XXI° Congr.Naz.Soc.It.Microbiologia,Roma,Dicembre 1985. Pag.439.
RONDININI G.,PESSINA A.,NERI M.G.,OTTOGALLI G.. Ricerca di enterobatteri produttori di tossina termolabile
in prodotti lattiero-caseario. Atti XXI° Congr.Naz.Soc.It.Microbiologia,Roma Dicembre 1985. Pag.549.
PESSINA A.,MUSCHIATO A.,NERI M.G.. Isolation of colony stimulating factors produced in vitro by murine adrenal
tumor cells. Commun. to the "Int.Symposium on Biological Regulation of cell proliferation.Milan, Marzo, 1986.
Pag.115.
MOCARELLI P.,BRAMBILLA P.,MAROCCHI A.,PESSINA A.,COLOMBO L.,TRAMACERE P.L.,SARTO C..
Purification of GM-CSF from the myoepithelioma cell line SGC-A. Commun. to the "Int. Symposium on Biological
Regulation of cell proliferation. Milano Marzo,1986.Pag.87.
PESSINA A.,NERI M.G.,MUSCHIATO A.,COCUZZA G.. Effetto della pefloxacina sulla proliferazione di precursori
mieloidi. Atti XV° Congr.Naz. Soc. It.Chemioterapia. Pesaro-Urbino Giugno 1987. Ed.CIC srl ,Roma.Pag-31-33.
PESSINA A.,NERI M.G.,MINEO E.,COCUZZA G.. Effect of lomefloxacin (LMFX) and ciprofloxacin(CPFX) on the
in vitro proliferation of granulocyte-macrophage precursors. Proc. 16th Int.Congr.Chemother.,Jerusalem June
1989.Pag.3131-3132.
PESSINA A.,MINEO E.,GRIBALDO L.,NERI MG. Cytotoxicity of fluoroquinolones on murine cell lines. Proc."5th
Int. Symposum on Clinical and Environmental Microbiology", Brescia,October 1991.Pagg.55-56.
ABSTRACTS AND COMMUNICATIONS TO CONGRESS ( about 100 not reported )
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