Programme PhD course - Maastricht University

Programme PhD / Master course
Advanced Microscopy and Vital Imaging
2014
Monday, June 2, 2014
Time
09.00 – 09.15
Item
Welcome and brief overview of the course
09.15 – 10.00
Frans Ramaekers
Dept. Molecular Cell Biology, Maastricht University Medical Center
Basics of Microscopy
10.15 – 12.45
Marc van Zandvoort
Dept. Molecular Cell Biology, Maastricht University Medical Center
Practical course: Bright field microscopy, a hands-on course
GROUP I
Location
UNS 50
F.5.111A
UNS 50
F.5.111A
UNS 40
C.4.577
Jos Broers/Helma Kuijpers [Marc van Zandvoort]
Maastricht University Medical Center
10.15 – 11.15
Fluorescence microscopy
GROUP II
UNS 50
F.5.111A
Marc van Zandvoort
Dept. Molecular Cell Biology, Maastricht University Medical Center
13.30 – 14.30
Fluorescence microscopy
GROUP I
UNS 50
F.5.111A
Marc van Zandvoort
Dept. Molecular Cell Biology, Maastricht University Medical Center
13.30 – 16.00
Practical course: Bright field microscopy, a hands-on course
GROUP II
Jos Broers/Helma Kuijpers [Marc van Zandvoort]
Maastricht University Medical Center
NVvM
UNS 40
C.4.577
Tuesday, June 3, 2014
Time
09.00 – 10.00
Item
Principles of confocal scanning laser microscopy
10.00 – 11.00
Edwin Lamers
Carl Zeiss Benelux
Live cell imaging using GFP-technology in combination with CSLM
11.00 – 12.00
14.00 – 15.00
11.00 – 14.00
Jos Broers
Dept. Molecular Cell Biology, Maastricht University Medical Center
Sample preparation
GROUPS 5-8
Frans Ramaekers
Dept. Molecular Cell Biology, Maastricht University Medical Center
Sample preparation
GROUPS 1-4
UNS 50
F.5.111A
UNS 50
F.5.111A
UNS 50
F.5.111A
Frans Ramaekers
Dept. Molecular Cell Biology, Maastricht University Medical Center
Demonstrations (according to schedule, 45 min per group)
GROUPS 1-4
Confocal scanning laser microscopy
14.00 – 17.00
Location
UNS 50
F.5.111A
Jos Broers / Helma Kuijpers
Dept. Molecular Cell Biology, Maastricht University Medical
Center
Demonstrations (according to schedule, 45 min per group)
GROUPS 5-8
Confocal scanning laser microscopy
Jos Broers / Helma Kuijpers
Dept. Molecular Cell Biology, Maastricht University Medical
Center
NVvM
UNS 50
G.5.223A
UNS 50
G.5.223A
Wednesday, June 4, 2014
Time
09.00 – 09.45
Item
Analysis of the neuromuscular junction – quantitative microscopy
09.45 – 10.30
Mario Losen
Dept. of Basic Neurosciences, Maastricht University Medical Center
Backgrounds and applications of STED Microscopy
Patrick van Wieringen
Leica Microsystems BV
Location
UNS 50
F.5.111A
UNS 50
F.5.111A
10.30 – 10.45
Coffee/tea break
UNS 50
F.5.111A
10.45 – 11.30
Two-photon laser microscopy for the three-dimensional study of
tissues
UNS 50
F.5.111A
11.45 – 13.15
15.00 – 16.30
11.45 – 17.00
Marc van Zandvoort
Dept. Molecular Cell Biology, Maastricht University Medical Center
ImageJ
GROUPS 5-7
Jos Broers
Dept. Molecular Cell Biology, Maastricht University Medical Center
ImageJ
GROUPS 1-4
Jos Broers
Dept. Molecular Cell Biology, Maastricht University Medical Center
Demonstrations (according to schedule, 45 min per group)
GROUPS 1-7
Two-photon scanning laser microscopy
Timo Rademakers [Zhuojun Wu]
Sanquin, Amsterdam
NVvM
UNS 50
F.5.111A
0f
Pc ruimte
UNS 50
F.5.111A
Of pc
ruimte
UNS 50
H.3.347
Thursday, June 5, 2014
Time
09.00 – 10.00
10.00 – 11.00
Item
Advances in multiphoton microscopy – in vivo imaging in
cardiovascular research
Location
UNS 50
F.5.111A
Timo Rademakers
Sanquin, Amsterdam
High throughput and high content cellular analysis for systems
biology
UNS 50
F.5.111A
Winnok De Vos
Ghent University
11.00 – 11.15
Coffee/tea break
11.15 – 12.15
Advanced Electron Microscopy
12.30
Peter Peters
EM-Unit and Dept. Molecular Cell Biology, Maastricht University
Medical Center
Departure to FEI Nanoport Company, Eindhoven
14.15 – 15.00
15.00 – 16.30
16.45
Peter Peters /Helma Kuijpers
EM-Unit and Dept. Molecular Cell Biology, Maastricht University
Medical Center
Welcome and introduction biological application of electron
microscopy
UNS 50
F.5.111A
UNS 50
(by bus)
Wim Busing
Sr Applications Specialist NanoBiology, FEI Company, Eindhoven
Demo's on Quanta 3D FEG (SEM), Quanta 250 (SEM), and Tecnai
20 (TEM)
Wim Busing
Sr Applications Specialist Nano Biology,FEI Company, Eindhoven
Departure back to Maastricht University Medical Center
NVvM
FEI,
Eindhoven
(by bus)