振動誘起流れによる卵子の搬送と機械的特徴量計測

振動誘起流れによる卵子の搬送と機械的特徴量計測
○学中原康正佐久間臣耶正早川健正新井史人
名古屋大学大学院工学研究科マイクロ・ナノシステム工学専攻
On-chip mechanical characterization
Pump-free transportation
Robot-integrated microfluidic chip
Cell mechanical
Vibration-induced flow
Sample No.1
Robochip
Force sensing
A local flow induced by
a circular vibration
Characterization system
S. Sakuma, et.al. 2013
Force applying
T. Hayakawa et. al. (2014)
Transportation and measurement in an open environment
Oocyte mechanical characterization with open-chip
Force sensor
Open microchannel
Directly accessible
・Bubble‐free measurement
・Easy to introduce/collect
(i)Introduction
Measurement point
Inlet
On-chip probe
(ii)Transportation
(iii)Measurement
Vibration-induced
flow
Syringe
Introduced oocyte
(iv)Collection
Force sensing
Syringe
Transported oocyte
Deformed oocyte
Push the oocyte
Fabrication process
Fabricated chip
Image of measurement point
Experiment
System configuration
Conclusion
Transportation velocity Mechanical characterization
1. We proposed a system that transports oocytes and
measures their mechanical characteristics with
open-chip.
2. 78 % of the measured oocytes were alive
(the viability of the control samples were 82 %)
Reference
Mouse oocyte after 1 hour thawed
The viability of measured oocytes:78 % (N=9)
(Control : 82 %)
Contact person： Kou Nakahara
E-mail: [email protected],
URL: http://www.biorobotics.mech.nagoya-u.ac.jp/
TEL: 052-789-5026, FAX : 052-789-5027
Acknowledgements：
本研究は科学研究費（15K13909）の支援をうけて行わ
れたものです．
Nakahara K, Sakuma S, Hayakawa T, and Arai F. “Onchip transportation and measurement of mechanical
characteristics of oocytes in an open environment”
Micromachines 6.6 (2015): 648-659

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