XF Cell Mito Stress Test Kit A complete profile of mitochondrial function The Gold Standard for Measuring Mitochondrial Function Functional mitochondrial measurements are key to understanding cellular activation, proliferation, differentiation and dysfunction. The XF Cell Mito Stress Test Kit provides a complete mitochondrial profile and reveals critical information not evident in basal metabolism measurements alone. This gold standard assay has been used in hundreds of publications to successfully advance the understanding of metabolism’s role in cellular physiology, disease pathology, and etiology. XF Cell Mito Stress Test Kit: • Run a complete assay from a single-use pouch • No need to aliquot • No DMSO needed, use media to re-suspend compounds • Rotenone and antimycin A are combined in a single tube Easy to Use | Fast Preparation Reproducible Results | The XF Cell Mito Stress Test Kit reveals the complete mitochondrial stress profile, and the report generator automatically calculates the most important metrics, simplifying your work. Now you can confidently quantify cellular bioenergetics, identify mitochondrial dysfunction, and measure cells’ response to stress. Mitochondrial Respiration Oligomycin XF Cell Mito Stress Test Profile Maximal Respiration Spare Respiratory Capacity Non-mitochondrial Respiration 320 OCR (pMoles/min) Proton Leak Antimycin A & Rotenone 360 Basal Respiration ATP Production FCCP 280 Spare Respiratory Capacity 240 200 Maximal Respiration 160 ATP Production 120 Basal 80 Respiration Proton Leak Non-mitochondrial Respiration 40 0 0 10 20 30 40 50 60 70 TIME (minutes) www.seahorsebio.com 80 90 100 110 XF Cell Mito Stress Test Kit Measure basal respiration, ATP production, proton leak, and spare respiratory capacity Each XF Cell Mito Stress Test Kit eliminates the questions and concerns of where to source the reagents, what concentrations are optimal, and best storage conditions. The XF Cell Mito Stress Test Kit contains six individual pouches each with enough reagents to run one 96- or 24-well microplate. With the reagents now ready at your fingertips, the easy-to-use and convenient XF Cell Mito Stress Test Kit is your ultimate tool for assay optimization. Ordering Information Product: XF Cell Mito Stress Test Kit Part Number: 103015-100 (replaces 101706-100) Kit Contents: Six single-use reagent pouches each containing oligomycin, FCCP and rotenone/animycin A Number of Assays: Six XFe96/24 or XF96/24 microplate assays Shipping Conditions: Ships at ambient temperature Storage Requirements: Store at -20°C Seahorse Bioscience Inc. 16 Esquire Road North Billerica, MA 01862 US +1-978-671-1600 800-671-0633 www.seahorsebio.com Seahorse Bioscience Europe Symbion Science Park, Boks 22 Fruebjergvej 3 2100 Copenhagen DK +45 31 36 98 78 Seahorse Bioscience Asia 199 Guo Shou Jing Road Suite 207 Pudong, Shanghai 201203 CN 0086 21 33901768 201202063
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