CLONING KITS & VECTORS lucigen.com/cloning Cloning the Unclonable: Meet our GC Vectors Greater stability, more clones with pSMART GC kits • Built-in transcriptional terminators stabilize difficult DNA sequences • More colonies. Greater success. • No more blue white screening. More recombinants, even Stabilize toxic inserts pSMART GC vectors are transcription- and No more cloning blues with tough-to-clone DNA translation-free, eliminating problems associated with cloning pSMART GC vectors do not Get more colonies and difficult or toxic DNA sequences into conventional vectors. contain a lacZ sequence for blue/white screening. For increase your chance of obtaining the correct clone. MW GC Cloning | TOPO TA standard inserts, the cloning MW efficiency using GC cloning Correct Inserts can be >99%. 1.6 × 106 1.2 × 106 8 × 105 4 × 105 0 GC HK TopoTA Compared to TOPO TA cloning, GC cloning produces more clones that contain the correct insert. Did you know? 3´ Taq polymerases leave a mixture of amplicons with dA and dG overhangs, not just dA. GC cloning vectors have dC overhangs which bind to amplicons with dG overhangs. The cloning efficiency using GC cloning can be >99%. Best of all, there’s no need to change anything about your PCR in order to use a GC vector from Lucigen. Lucigen Corporation Phone: 608 831 9011 Toll Free: 888 575 9695 Fax: 608 831 9012 www.lucigen.com [email protected] [email protected] For research use only. Not for human or diagnostic use. FM 598408 C Vector 5´ G 3´ 5´ 3´ G PCR Product 5´ C 5´ Vector 3´ GC Cloning. EconoTaq™ or other non-proofreading DNA polymerase adds a single G overhang to the PCR products. Ligation to the complementary C-overhang pSMART GC Vector is fast and highly efficient. C C CLONING KITS & VECTORS SL1 primer SR2 primer lucigen.com/cloning Ori Kan pSMART ® GC LK Maximum insert stability with pJAZZ® OK-GC • Easily clone any G-tailed DNA up to 30 kb ROP pJAZZ®-OK GC • Novel, linear vector • Clone challenging DNA sequences: repetitive, G/C-rich, A/T-rich C tel N repA G INSERT G G Kanr Insert cB C G CC Fig. 1 The pJAZZ OK-GC vector is linear to prevent supercoiling, helping stabilize your insert. SL1 primer SR2 primer Ori Stabilizing your most difficult inserts has never been easier. The pJAZZ pSMART GC HK DNA sequences. The vector contains a single 3’-C overhang which is OK-GC vector employs Lucigen’s exclusive CloneSmart™ transcription- compatible with the 3’-G overhang generated by non-proofreading free technology that reduces cloning bias and maximizes efficiency. polymerases, such as Taq (Figure 1). Efficiently and easily clone PCR Challenging inserts are stabilized because pJAZZ OK-GC is linear and products up to 30 kb! Ka n ® does not supercoil, avoiding torsional stress caused by structure rich G SP6 • Contains lacZ fragment for blue/white screening Ka GC Cloning vs TOPO TA Cloning the benefit of insert stability? Lucigen’s pGC Blue White CFU vector contains a lacZ sequence to allow blue/ polymerase promoters for in vitro transcription of the cloned insert (Figure 1). Compared to 1.2 × 106 8 × 105 4 × 105 0 increasing your success for those tough-to-clone GC Blue Topo TA DNA sequences (Figure 2). Fig. 2 Compared to TOPO TA cloning, pGC Blue generated more colonies, increasing the likelihood you’ll obtain the clone you need. Lucigen Corporation Phone: 608 831 9011 Toll Free: 888 575 9695 Fax: 608 831 9012 www.lucigen.com [email protected] [email protected] For research use only. Not for human or diagnostic use. FM 598408 T7 nr Fig. 1 pGC Blue contains a lacZ sequence for blue/white screening. Transcriptional terminators flank the lacZ gene to improve insert stability. 1.6 × 106 TOPO TA cloning, you’ll generate more colonies, lacZ Blue CFU 2.0 × 106 CFU/Ligation white screening, as well as dual opposed RNA C pGC™ Blue • Transcriptional terminators flank the cloning site for insert stability Want the option of blue/white screening with C Ori Still prefer blue? Try pGC™ Blue plac INSERT G
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