MOG-401 - IQ Education

www.iqeducation.in
Name : ……………………………………………………………
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Roll No. : ………………………………………………………..
Invigilator’s Signature : ………………………………………..
CS/B.Sc.(H)/BT/GEN/MICRO-BIO/MOL-BIO/SEM-4/MOG-401/2013
2013
MOLECULAR GENETICS
Time Allotted : 3 Hours
Full Marks : 70
The figures in the margin indicate full marks.
pap
Candidates are required to give their answers in their own words
as far as practicable.
wb
er.
GROUP – A
( Multiple Choice Type Questions )
1.
Choose the correct alternatives for any ten of the following :
i)
10
1 = 10
Which of the following methods is most useful for
enzymatic amplification of specific segment of DNA ?
Nucleotide sequencing
b)
DNA hybridization
c)
PCR
d)
None of these.
in
a c.
ut.
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a)
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CS/B.Sc.(H)/BT/GEN/MICRO-BIO/MOL-BIO/SEM-4/MOG-401/2013
ii)
Gene expression is analyzed by
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iii)
a)
Southern blot
b)
Restriction digestion
c)
Northern blot
d)
None of these.
In sourthern blotting experiment,
the
binding
of
transferred DNA to the nylon membrane is ……………
type.
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iv)
a)
ionic
b)
covalent
c)
hydrophobic
d)
van der Waals.
DNA hybridization is a technique, which relies on the
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following properties of DNA, except
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Double strandedness
b)
sequence specificity
c)
major and minor grooves
d)
denaturation-renaturation properties.
in
a c.
ut .
v)
a)
For RNA detection which PCR is best ?
a)
Nested PCR
b)
RT-PCR
c)
ARMS-PCR
d)
MS-PCR.
2
CS/B.Sc.(H)/BT/GEN/MICRO-BIO/MOL-BIO/SEM-4/MOG-401/2013
vi)
In Western blot the protein samples are run on
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vii)
a)
Agarose gel
b)
polyacrylamide gel
c)
formaldehyde-agarose gel
d)
none of these.
The stringency of a hybridization reaction depends on
all of the following, except
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NaCl concentration
b)
type of reporter molecule
c)
nucleotide sequence of probe
d)
pH.
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a)
viii) Ligase joins two DNA molecules together by forming a
covalent bond between
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two PO 4
b)
two OH
c)
one PO 4 and one OH
d)
Complementary nucleotides on opposite strands.
-lactum ring is present in
in
a c.
ut .
ix)
a)
a)
Tetracycline
b)
Ampicillin
c)
Kanamycin
d)
Streptomycin.
3
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x)
Principal
function
of
reporter
molecule
in
DNA
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hybridization assay
aid in base pairing
c)
aid in the detection of probe target hybridization
d)
bind the target DNA to the solid support.
More stringent washing condition is selected when
a)
Probe has lower affinity with the DNA
b)
Probe has higher affinity with the DNA
c)
Probe is radio-labelled
d)
Prove is non-radio-labelled/enzyme labelled.
Microsatellites are
in
a c.
ut .
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b)
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xii)
enhance the stringency of hybridization reaction
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xi)
a)
a)
frequently found in bacterial genomes
b)
always smaller than 50 bp
c)
used as DNA marker
d)
movable DNA elements.
4
CS/B.Sc.(H)/BT/GEN/MICRO-BIO/MOL-BIO/SEM-4/MOG-401/2013
GROUP – B
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( Short Answer Type Questions )
Answer any three of the following.
3
5 = 15
2.
Write a short note on c DNA library.
3.
Write the basic differences between capillary transfer and
electro-transfer in sourthern blot.
Define cloning vector. What should be the properties of a
pap
4.
good vector ?
5.
2+3
Why Type-II restriction enzymes are considered to be the
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most useful in molecular biology ? How it is different from
Type-I ?
6.
3+2
What is MCS in a vector ? Why alkaline phosphatase
treatment is needed in cloning ? What is linker ? How it
7.
What is Directional Cloning ? When you use this ? Where it
is different from conventional cloning ?
8.
1+2+1+1
in
a c.
ut .
differs from adapters ?
2+1+2
What do you mean by Restriction Modification System in
bacteria ? Explain.
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5
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GROUP – C
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( Long Answer Type Questions )
Answer any three of the following.
9.
3
15 = 45
What is the basic structure of plasmid vector ? Explain the
regulation of expression vector. What are steps of gene
cloning ? What are the characteristics of YAC and BAC ?
3+5+3+4
pap
10. Write short notes on any three of the following :
3
Sanger and Coulson method for DNA sequencing
b)
Protein Blotting
c)
Microarray
d)
Inverse PCR.
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a)
5
11. What is Agarose ? PCR amplification is based on which
theory ? Briefly discuss the steps of PCR with suitable
in
a c.
ut .
diagram. Write down the applications of PCR. Briefly explain
the efficiency of PCR.
2+1+4+4+4
12. What is a probe ? How they are classified ? What is Dot Blot
technique ? Briefly describe the technique of RFLP and
Asymmetric PCR.
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2+3+4+6
6
CS/B.Sc.(H)/BT/GEN/MICRO-BIO/MOL-BIO/SEM-4/MOG-401/2013
13. Write short notes on any three of the following :
3
5
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a)
RAPD
b)
M13 vector
c)
Blue-White screening
d)
T-DNA transfer technique
e)
Southern Blotting.
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7
in
a c.
ut .
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Name : ……………………………………………………………
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Roll No. : ……………………………………………..…………..
Invigilator's Signature : ………………………………………..
CS/B.Sc.(H)/BT/GENE/MOLBIO/MICROBIO/SEM-4/MOG-401/2012
2012
MOLECULAR GENETICS
Time Allotted : 3 Hours
Full Marks : 70
pap
The figures in the margin indicate full marks.
Candidates are required to give their answers in their own words as
far as practicable.
GROUP – A
wb
er.
( Multiple Choice Type Questions )
1.
Choose the correct alternatives for any ten of the following :
i)
10 × 1 = 10
Type II restriction enzymes have which of the following
characteristics ?
Repetitive sequence
b)
c)
Palindromic sequence d)
Conserved sequence
in
a c.
ut.
ii)
a)
None of these.
Which of the following parameters is
taken in
consideration during primer designing ?
4701
a)
Length of DNA
b)
GC content
c)
Both (a) & (b)
d)
None of these.
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iii)
Which one requires NAD+ ?
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iv)
vi)
b)
E.coli DNA ligase
c)
Both of these
d)
None of these.
What does PUC stand for ?
a)
Produced by the University of California
b)
Produced by the University of Cambridge
c)
Produced by the University of Colombia
d)
Produced by the University of Cornell.
Cloning requires all the following, except
a)
Restriction enzyme
b)
DNA ligase
c)
Methylase
d)
Vector.
BamH1 is a ................. type of restriction enzyme.
a)
Type I
c)
Type III
b)
Type II
d)
None of these.
wb
er.
vii)
T4 DNA ligase
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v)
a)
In southern blotting experiment, the
binding of
transferred DNA to the Nitrocellulose membrane is
............... type.
ionic
c)
hydrophobic
b)
covalent
d)
van der Waals.
in
a c.
ut .
a)
viii) If a probe length is less than 200 b.p. ............. is
most useful.
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a)
Nylon membrane
b)
Nitrocellulose membrane
c)
Whatman filter paper
d)
None of these.
2
CS/B.Sc.(H)/BT/GENE/MOLBIO/MICROBIO/SEM-4/MOG-401/2012
ix)
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Denaturants such as formamide cause ds DNA to melt
by
a)
b)
x)
xii)
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xi)
decreasing the repulsion between phosphates
increasing the hydrophobic interactions between
bases
c)
increasing the repulsion between the phosphates
d)
decreasing the hydrophobic interactions between
bases.
The technique of RT-PCR is best suitable for detection
of
a)
Allele
b)
Pathogen
c)
Amplicon
d)
RNA.
Which one is a reporter gene ?
a)
Lac Z
b)
Luciferase
c)
CAT
d)
All of these.
Which one is employed in the DNA hybridization
assay ?
a)
Etbr
b)
Enzyme
c)
Chemiluminiscent moiety
d)
None of these.
GROUP – B
( Short Answer Type Questions )
Answer any three of the following.
3 × 5 = 15
in
a c.
ut .
2.
Describe Dot blot hybridization.
3.
What is linker and its application ? How is it different from
Adaptors ?
4.
Write short notes on the following :
a)
M13 Phage vector.
b)
Micro-array
c)
Nested PCR
d)
RAPD.
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3
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5.
Describe multiplex PCR.
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6.
What is SDS PAGE electrophoresis ? What is the significance
of using SDS in this technique ?
GROUP – C
( Long Answer Type Questions )
Answer any three of the following.
7.
3 × 15 = 45
What is Cloning ? Write down the steps of gene cloning. Why
Alkaline Phosphate treatment is needed in cloning ? Write a
short note on Nick Translation.
pap
8.
2+5+3+5
What are the properties of good vector ? What is Blue-White
screening ? What is Agobacterium based plasmid vector ?
Discuss the mechanism of T-DNA transfer.
9.
4+4+3+4
Describe Colony Hybridization techniques. What is Protein
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blotting Technique ? Briefly describe the technique. What
kind of probe is used in Southern blotting ?
5+2+6+2
10. Write short notes on any three of the following :
Maxam and Gilber's chemical degradation method.
b)
Gel Electrophoresis.
c)
Sanger and Coulson method of DNA sequencing
d)
Site Directed mutagenesis.
in
a c.
ut .
a)
11. Write short notes on any three of the following :
a)
Nested PCR
b)
Insertional inactivation
c)
Red white screening
d)
Site directed mutagenesis.
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4
3 × 5 = 15
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Name : ……………………………………………………………
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Roll No. : ……………………………………………..…………..
Invigilator’s Signature : ………………………………………..
CS/B.Sc (H), Genetics, MolBio, MicroBio, BT/SEM-4/MOG-401/2011
2011
MOLECULAR GENETICS
Time Allotted : 3 Hours
Full Marks : 70
The figures in the margin indicate full marks.
pap
Candidates are required to give their answers in their own words
as far as practicable.
GROUP – A
( Multiple Choice Type Questions )
Choose
the
correct
following :
i)
ii)
4701
alternatives
for
any
ten
of
the
10 × 1 = 10
Cloning requires all the following except
a)
restriction enzyme
b)
DNA ligase
c)
methylase
d)
vector.
EcoR1 is a what type of restriction enzyme ?
a)
Type I
c)
Type III
‘ARS’ stands for
b)
Type II
d)
None of these.
in
a c.
ut.
iii)
wb
er.
1.
a)
Artificially Replicating Sequence
b)
Autonomously Replicating Sequence
c)
Automatically Replicating Sequence
d)
None of these.
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CS/B.Sc (H), Genetics, MolBio, MicroBio, BT/SEM-4/MOG-401/2011
iv)
Which one is employed in the DNA hybridization assay ?
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v)
b)
Enzyme
c)
Chemiluminicent moiety
d)
None of these.
Which of the following method is most useful for
enzymatic amplification of specific segment of DNA ?
a)
Nucleotide sequencing
b)
DNA hybridization
c)
PCR
d)
None of these.
Gene expression can be analyzed by
a)
Southern Blot
b)
Restriction Digestion
c)
Northern Blot
d)
None of these.
wb
er.
vii)
Etbr
pap
vi)
a)
In Southern Blotting experiment, the binding of
transferred DNA to the Nitrocellulose Membrane is
.................... type.
a)
Ionic
c)
Hydrophobic
b)
Covalent
d)
van der Waal.
viii) DNA hybridization is a technique, which relies on the
following properties of DNA, except
4701
in
a c.
ut .
ix)
a)
double strandedness
b)
sequence specificity
c)
major and minor grooves
d)
denaturation-renaturation properties.
β-lactum ring is present in
a)
Tetracycline
b)
Ampicillin
c)
Kanamycin
d)
Streptomycin.
2
CS/B.Sc (H), Genetics, MolBio, MicroBio, BT/SEM-4/MOG-401/2011
x)
For RNA detection which PCR is best ?
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xi)
a)
Nested PCR
b)
RT-PCR
c)
ARMS-PCR
d)
MS-PCR.
In Southern Blotting
transferred DNA to
.................... type.
experiment, the binding
the Nylon membrane
a)
Ionic
b)
Covalent
c)
Hydrophobic
d)
van der Waal.
of
is
xii) In Western Blot the protein samples are run on
Agarose gel
b)
Ployacrylamide gel
c)
Formaldehyde-agarose gel
d)
None of these.
pap
a)
wb
er.
GROUP – B
( Short Answer Type Questions )
Answer any three of the following.
2.
3 × 5 = 15
Why are Type II restriction enzymes considered to be the
most useful in molecular biology ? How is it different from
Type I ?
3+2
Write a short note on c-DNA library.
4.
What do you mean by restriction modification system in
bacteria ? Explain.
5.
Write the basic differences between Capillary transfer and
Electro-transfer in Southern Blot.
6.
Define cloning vector. What should be the properties of a
good vector ?
4701
in
a c.
ut .
3.
1+4
3
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CS/B.Sc (H), Genetics, MolBio, MicroBio, BT/SEM-4/MOG-401/2011
GROUP – C
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( Long Answer Type Questions )
Answer any three of the following.
3 × 15 = 45
What components involved in cloning ? Describe the cell
based DNA cloning process with suitable diagram. What are
the usefulness of M13 vector ? What are the characteristics
of YAC and BAC vector ?
4+5+2+4
8.
Describe the steps ( preferably with diagram ) involved in
PCR mentioning the appropriate temperature. Write the
advantages of PCR over cloning.
10 + 5
9.
What is Southern Blotting ? Who invented it ? Write the
basic steps involved in Southern Blotting ? What can you use
as a probe in Southern Blotting ?
2 + 1 + 10 + 2
pap
7.
What are the necessities of probe labelling in all blotting
experiments ?
b)
What are the different types of labelling techniques for
the probe ?
c)
Explain the process for developing probes to identify the
pathogens.
d)
Give an example of non-radio labelling of probe.
wb
er.
10. a)
4701
a)
In vitro DNA cloning
b)
Nick translation
c)
Directional cloning
d)
Plaque hybridization.
4
3×5
in
a c.
ut .
11. Write short notes on any three of the following :
3+4+6+2
Name : ……………………………………………………………
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Roll No. : ………………………………………………………..
Invigilator's Signature : ………………………………………..
CS/B.Sc.(H), BT, Mol-Bio, Micro-Bio, Genetics/SEM-4/MOG-401/2010
2010
MOLECULAR GENETICS
Time Allotted : 3 Hours
Full Marks : 70
The figures in the margin indicate full marks.
pap
Candidates are required to give their answers in their own words
as far as practicable.
Graph sheet(s) will be provided by the Institution.
wb
er.
GROUP – A
( Multiple Choice Type Questions )
1.
Choose the correct alternatives for any ten of the following :
10 × 1 = 10
i)
iii)
4034
a)
restriction enzyme
b)
DNA ligase
c)
methylase
d)
vector.
EcoR1 is a ……………… type of restriction enzyme.
in
a c.
ut .
ii)
Cloning requires all the following except
a)
type I
b)
type II
c)
type III
d)
none of these.
Which one of the following is used
transformation ?
a)
CaCl 2
b)
Na 2 CO 3
c)
SnCl 2
d)
NaCl.
in bacterial
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CS/B.Sc.(H), BT, Mol-Bio, Micro-Bio, Genetics/SEM-4/MOG-401/2010
iv)
‘ARS’ is
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v)
b)
autonomously replicating sequence
c)
automatically replicating sequence
d)
none of these.
Which one of the following is employed in the DNA
hybridization assay ?
a)
Etbr
b)
Enzyme
c)
Chemiluminicent moiety
d)
none of these.
Which of the following methods is most useful for
enzymatic amplification of specific segment of DNA ?
a)
Nucleotide sequencing
b)
DNA hybridization
c)
PCR
d)
none of these.
wb
er.
vii)
artificially replicating sequence
pap
vi)
a)
Gene expression can be analyzed by
a)
southern blot
b)
restriction disation
c)
northern blot
d)
none of these.
viii) Which of the following parameters
consideration during primer designing ?
x)
4034
taken
a)
Length of DNA
b)
GC content
c)
Both (a) and (b)
d)
None of these.
in
in
a c.
ut .
ix)
is
Bam H1 is considered to be which categories of
restriction enzymes ?
a)
Type I
b)
Type II
c)
Type III
d)
None of these.
For RNA detection which PCR is best ?
a)
Nested PCR
b)
RT-PCR
c)
ARMS-PCR
d)
MS-PCR.
2
CS/B.Sc.(H), BT, Mol-Bio, Micro-Bio, Genetics/SEM-4/MOG-401/2010
xi)
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In southern blotting experiment, the binding of
transferred DNA to the Nylon membrane is ……………
type.
a)
ionic b)
covalent
c)
hydrophobic
d)
vander wall.
In southern blotting experiment, the binding of
transferred DNA to the Nitrocellulose membrane is
…………… type.
a)
ionic b)
covalent
c)
hydrophobic
d)
vander wall.
xii)
pap
2.
GROUP – B
( Short Answer Type Questions )
Answer any three of the following.
3 × 5 = 15
Why are type II restriction enzymes considered to be the
most useful in molecular biology ? How is it different from
type I ?
3+2
What is MCS in a vector ? Why alkaline phosphatase
treatment is needed in cloning ? What is linker ? How it
differs from adapters ?
1+2+1+1
4.
What is directional cloning ? When you use this ? Where is
it different from conventional cloning ?
2+1+2
5.
Write down the steps involved in the extraction of genomic
DNA from blood.
6.
What do you mean by restriction modification system in
bacteria ? Explain.
GROUP – C
( Long Answer Type Questions )
Answer any three of the following.
3 × 15 = 45
What characteristics cloning vectors have ? What is the
criteria for choosing any cloning vector ? What is the utility
of having antibiotic resistant markers in vectors ? What is
blue-white screening and how you can screen the successful
transformed cells carrying both the vectors and insert from
those carrying only vectors by this method ?
4+2+2+2+5
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3
in
a c.
ut .
7.
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3.
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CS/B.Sc.(H), BT, Mol-Bio, Micro-Bio, Genetics/SEM-4/MOG-401/2010
8.
What components involved in cloning ?
How is a foreign
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DNA being inserted into a host cell ?
characteristics of vector ?
What are the
Write the approx. insert size of
Phage, Cosmid, BAC, YAC vectors.
9.
4+3+4+4
Briefly describe the steps ( preferably with diagram ) involved
in PCR mentioning the appropriate temperature at each step.
How is annealing temperature related to Tm of your DNA
sample ? Write the advantages of PCR over cloning. 7 + 3 + 5
pap
10. What is PCR ? Who invented it ? Why is it useful in modern
biology ? How can you introduce a mutation in your DNA
sample be PCR ( Assume that you know the sequence of the
DNA sample ) ?
2+1+4+8
wb
er.
11. Why you need to label your probe before using in all blotting
experiment ?
What are the different types of labeling
techniques for the probe ?
How can you introduce the
following labeling in the probe :
|
5 labeling
b)
3 labeling
c)
Internal labeling.
|
in
a c.
ut .
a)
Give an example of non-radio labeling of probe.
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4
3+4+6+2
MOLECULAR GENETICS ( SEMESTER - 4 )
CS/B.Sc (H) (BT, Genetics, Mol.bio, Microbio)/SEM-4/MOG-401/09
www.iqeducation.in
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1. ………………………………
Signature of Invigilator
2. ………………………………
Reg. No.
Signature of the Officer-in-Charge
Roll No. of the
Candidate
––––––––––––––––––––––––––––––––––––––––––––––––––––––––
CS/B.Sc (H) (BT, Genetics, Mol.bio, Microbio)/SEM-4/MOG-401/09
ENGINEERING & MANAGEMENT EXAMINATIONS, JUNE – 2009
MOLECULAR GENETICS ( SEMESTER - 4 )
pap
Time : 3 Hours ]
[ Full Marks : 70
INSTRUCTIONS TO THE CANDIDATES :
2.
3.
4.
5.
6.
9.
This Booklet is a Question-cum-Answer Booklet. The Booklet consists of 32 pages. The questions of this
concerned subject commence from Page No. 3.
a)
In Group – A, Questions are of Multiple Choice type. You have to write the correct choice in the box
provided against each question.
b)
For Groups – B & C you have to answer the questions in the space provided marked ‘Answer
Sheet’. Questions of Group – B are Short answer type. Questions of Group – C are Long answer
type. Write on both sides of the paper.
Fill in your Roll No. in the box provided as in your Admit Card before answering the questions.
Read the instructions given inside carefully before answering.
You should not forget to write the corresponding question numbers while answering.
Do not write your name or put any special mark in the booklet that may disclose your identity, which will
render you liable to disqualification. Any candidate found copying will be subject to Disciplinary Action
under the relevant rules.
Use of Mobile Phone and Programmable Calculator is totally prohibited in the examination hall.
You should return the booklet to the invigilator at the end of the examination and should not take any
page of this booklet with you outside the examination hall, which will lead to disqualification.
Rough work, if necessary is to be done in this booklet only and cross it through.
in
a c.
ut.
7.
8.
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1.
No additional sheets are to be used and no loose paper will be provided
FOR OFFICE USE / EVALUATION ONLY
Marks Obtained
Group – A
Question
Number
Marks
Obtained
…………………………………………………………
Head-Examiner/Co-Ordinator/Scrutineer
4440 (04/06)
Group – B
Group – C
Total
Marks
Examiner's
Signature
CS/B.Sc (H) (BT, Genetics, Mol.bio, Microbio)/SEM-4/MOG-401/09
2
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pap
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in
a c.
ut .
4440 (04/06)
CS/B.Sc (H) (BT, Genetics, Mol.bio, Microbio)/SEM-4/MOG-401/09
3
ENGINEERING & MANAGEMENT EXAMINATIONS, JUNE – 2009
MOLECULAR GENETICS
SEMESTER – 4
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Time : 3 Hours ]
[ Full Marks : 70
GROUP – A
( Multiple Choice Type Questions )
1.
Choose the correct alternatives for any ten of the following :
i)
Cloning requires all the following, except
a)
Restriction enzyme
b)
DNA ligase
c)
Methylase
d)
Vector.
EcoR1 is a ............... type of restriction enzyme.
a)
Type I
c)
Type III
wb
er.
iii)
b)
Type II
d)
None of these.
Which one of the following is used in bacterial transformation
CaCl2
c)
SnCl2
‘ARS’ is
b)
Na 2 CO3
d)
NaCl.
!
Artifically Replicating Sequence
in
a c.
ut .
!
a)
! a)
v)
pap
ii)
iv)
10 × 1 = 10
b)
Autonomously Replicating Sequence
c)
Automatically Replicating Sequence
d)
None of these.
The stringency of a hybridization reaction depends on all of the following, except
a)
NaCl concentration
b)
Type of reporter molecule
c)
Nucleotide sequence of probe
d)
pH.
4440 (04/06)
CS/B.Sc (H) (BT, Genetics, Mol.bio, Microbio)/SEM-4/MOG-401/09
vi)
a)
enhance the stringency of hybridization reaction
b)
aid in base pairing
c)
aid in the detection of probe target hybridization
d)
bind the target DNA to the solid support.
Which one is employed in the DNA hybridization assay ?
a)
Etbr
b)
Enzyme
c)
Chemiluminiscent moiety
d)
None of these.
pap
viii)
Principal function of reporter molecule in DNA hybridization assay
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vii)
Which of the following methods is not useful for enzymatic amplification of
a)
Nucleotide sequencing
b)
DNA hybridization
c)
PCR
d)
None of these.
b)
restriction digestion
Gene expression can be analyzed by
a)
southern blot
c)
northern blot
d)
in
a c.
ut .
x)
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er.
specific segment of DNA ?
ix)
4
none of these.
In southern blotting experiment, the binding of transferred DNA to the
Nitrocellulose membrane is ............... type.
xi)
a)
ionic
b)
c)
hydrophobic
d)
covalent
van der Walls.
In West Blot, the protein samples are run on
a)
agarose gel
b)
c)
formaldehyde-agarose gel
d)
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ployacrylamide gel
none of these.
CS/B.Sc (H) (BT, Genetics, Mol.bio, Microbio)/SEM-4/MOG-401/09
xii)
produced by University of California
b)
produced by University of Cornell
c)
produced by University of Colombia
d)
produced by University of Cambridge.
BamH1 is considered to be which categories of restriction enzyme ?
a)
Type I
b)
Type II
c)
Type III
d)
None of these.
Which one is a reporter gene ?
a)
Lac Z
c)
CAT
b)
Luciferase
d)
All of these.
wb
er.
xv)
a)
pap
xiv)
What does pUC stands for ?
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xiii)
5
For RNA detection which PCR is best ?
a)
Nested PCR
c)
ARMS-PCR
b)
RT-PCR
d)
MS-PCR.
GROUP – B
in
a c.
ut .
( Short Answer Type Questions )
Answer any three of the following questions.
3 × 5 = 15
2.
Mention 5 restriction enzymes indicating the type of ends they produce in DNA.
3.
Write short note on Micro-array.
4.
What do you mean by Restriction Modification System in bacteria ? Explain.
5.
Write short note on c-DNA library.
6.
How can you introduce radio-labelling in your DNA ?
4440 (04/06)
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6
GROUP – C
( Long Answer Type Questions )
Answer any three of the following questions.
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htt
7.
3 × 15 = 45
What characteristics cloning vectors have ? What is the criteria for choosing any
cloning vector ? What is the utility of having antibiotic resistant markers in vectors ?
What is Blue-White screening ? How can you screen the successful transformed cells
carrying both the vectors and insert from those carrying only vectors by this
method ?
8.
4+2+2+2+5
Briefly describe the steps ( preferably with diagram ) involved in PCR mentioning the
pap
appropriate temperature at each step. How annealing temperature is related to Tm of
your DNA sample ? Write the advantages of PCR over cloning.
9.
7+3+5
Why do you need to label your probe before using in all blotting experiment ? What are
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er.
the different types of labelling techniques, for the probe ? How can you introduce the
following labelling in the probe
a)
5’ labelling
b)
3’ labelling
c)
Internal labelling.
Give an example of non-radio labelling of probe.
in
a c.
ut .
10.
3+4+6+2
What is Southern Blotting ? How is it different from Northern and Western Blotting ?
Write the basic steps involved in southern blotting. What can you use as a probe in
southern blotting ?
11.
2+4+7+2
What is PCR ? Who invented it ? Why is it useful in modern biology ? How can you
introduce a mutation in your DNA sample be PCR ( Assume that you know the
sequence of the DNA sample ) ?
2+1+4+8
END
4440 (04/06)

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