BCH 6415 - Department of Biochemistry and Molecular Biology

BCH 6415
Advanced M olecular and Cell Biology
3 Credits
Spring Semester 2015
This course is designed for advanced graduate students and highly advanced undergraduates who already have taken a
one semester graduate level survey course in molecular biology (or molecular genetics) and desire a course on current
aspects of molecular biology. Lectures and discussions will be based entirely upon the current scientific literature on
transcription, RNA processing, protein synthesis, post-translational regulation, DNA replication, DNA repair, and
DNA recombination, and emphasize experimental approaches to understanding these cellular processes. Students
should have a good working knowledge of molecular biology and be able to comprehend articles on these topics in
journals such as Cell, Science, Nature, Genes and Development, etc. No general molecular biology textbook will cover
topics in this course in sufficient detail and depth, though introductory background information may be available in
textbooks such as:
Lodish et al. Molecular Cell Biology
Alberts et al. Molecular Biology of the Cell
Cox et al. Molecular Biology: Principles & Practice Weaver Molecular Biology
Ample use will be made of handouts and reading lists of relevant scientific articles throughout the course; much of the
material in handouts will be taken from articles in the current scientific literature. Each instructor lectures in areas of
his/her research expertise. Whenever possible, instructors will provide handouts of their lectures, but students are
responsible for obtaining their own copies of the assigned (and optional) reading from scientific journals. The slides
from each lecture will be available on the UF e-Learning website.
Formal lectures and discussions will be held 5th period in Room R3-265 in the Academic Research Building on
Monday, Wednesday, and Friday, 11:45 am to 12:35 p.m.
COURSE I NSTRUCTORS:
Dr. Thomas Yang (Coordinator)
Dept. Biochemistry & Molec. Biol.
R3-295, 392-6472
[email protected]
Dr. Maurice Swanson
Dept. Molec. Genetics & Microbiol.
Cancer/Genetics Bldg., 273-8076
[email protected]
Dr. Linda Bloom
Dept. Biochem. & Molecular Biol.
R3-165, 392-8708
[email protected]
Dr. Michelle Gumz
Dept of Medicine
Division of Nephrology
[email protected]
Dr. James B. Flanegan
Dept. Biochem. & Molecular Biol.
R3-234A, 392-0688
[email protected]
Dr. Rolf Renne
Dept. Molec. Genetics & Microbiol.
Cancer/Genetics Bldg., 273-8204
rrenne.ufl.edu
Dr. Kevin Brown
Dept. of Biochem. & Molec. Biol.
Cancer/Genetics Bldg.
Rm 392C, 273-5458
[email protected]
Dr. Suming Huang
Dept. of Biochem. & Molec. Biol.
Cancer/Genetics Bldg.
Rm 355, 273-8199
[email protected]
EXAM S:
Three examinations periods are scheduled during the semester for Thurs February 12 and Tues March 24 at 6:00 –
8:00 pm in Room R3-265 of the Academic Research Building, plus the Final Exam. The final exam is NOT
cumulative. Please arrange your schedules to enable you to attend these sessions. There will be NO make-up exams.
GRADES:
Grades will be based on the three exams which will be worth 100 points each. The final exam is NOT cumulative and
will cover only the material from the final third of the course.
BCH 6415 – Spring 2015
Lecture Schedule
DATE
DAY
Jan 7
Wed
Jan 9
Fri
Jan 12
Mon
Jan 14
Wed
Jan 16
Fri
Jan 19
Mon
Jan 21
Wed
Jan 23
Fri
Jan 26
Mon
Jan 28
Wed
Jan 30
Fri
Feb 2
Mon
Feb 4
Wed
Feb 6
Fri
Feb 9
Mon
Feb 11
Wed
Feb 12
Thurs
Feb 13
Fri
Feb 16
Mon
Feb 18
Wed
Feb 20
Fri
Feb 23
Mon
Feb 25
Wed
Feb 27
Fri
FEB 28 – MARCH 7
March 9
Mon
March 11
Wed.
March 13
Fri
March 16
Mon
March 18
Wed
March 20
Fri
March 23
Mon
March 24
Tues
March 25
Wed
March 27
Fri
March 30
Mon
April 1
Wed
April 3
Fri
April 6
Mon
April 8
Wed
April 10
Fri
April 13
Mon
April 15
Wed
April 17
Fri
April 20
Mon
April 22
Wed
(To Be Scheduled)
TOPIC
RNA Polymerase Structure & Function
Genomics: Technology & ENCODE
Nucleosomes & Chromatin I
Nucleosomes & Chromatin II
Basal Transcription
NO CLASSES – MARTIN LUTHER KING DAY
Chromatin Remodeling I
Chromatin Remodeling II
Histone Modification I
Histone Modification II
Co-Activators/Co-Repressors
Activation of Transcription
Transcription Elongation I
Transcription Elongation II
MAR's, Insulators, Transvection
Nuclear Organization, Transcription Factories
EXAM – 6:00-8:00 p.m.; Lectures through 2/9
Locus Control Regions
Gene Regulation by Long Non-Coding RNA's
Pol I and III Transcription
Protein Synthesis I
Protein Synthesis II
Post-Translational Regulation
RNA Processing I
SPRING BREAK
RNA Processing II
RNA Splicing I
RNA Splicing II
RNAi & Micro RNA’s
DNA Methylation & Epigenetic Regulation
DNA Topology
DNA Replication: Initiation & Origins
EXAM – 6:00-8:00 p.m.; Lectures through 3/18
DNA Replication – Mechanisms I
DNA Replication – Mechanisms II
DNA Replication: Fidelity
Mismatch Repair
Mechanisms of DNA DSB Repair
Completing Replication & Telomeres
DNA Damage & Excision Repair
Homologous Recombination
Lesion Bypass
Damage Response I
Damage Response II
Viral Replication I
Viral Replication II
FINAL EXAM (Non-Cumulative)
INSTRUCTOR
Yang
Yang
Yang
Yang
Huang
Yang
Yang
Huang
Huang
Huang
Huang
Yang
Yang
Huang
Huang
Huang
Huang
Huang
Gumz
Gumz
Gumz
Yang
Yang
Swanson
Swanson
Renne
Yang
Bloom
Bloom
Bloom
Bloom
Bloom
Brown
Brown
Bloom
Bloom
Bloom
Bloom
Brown
Brown
Flanegan
Flanegan