Impacts of PAH-contaminated sediment on early life history stages of benthic fish John Incardona Conservation Medicine Group Ecotoxicology and Environmental Fish Health Program NOAA Northwest Fisheries Science Center Seattle Screening high molecular weight PAHs SF Bay, RMP 2003 15 Kitimat Arm 2004 10 PER DBA IDP BEP BAP BkF BbF CHR BAA PYR FLA ANT PHN DBT FLU ACE ACY 0 BPH 5 NPH percent total 20 Screening high molecular weight PAHs SF Bay, RMP 2003 15 Kitimat Arm 2004 10 PER DBA IDP BEP BAP BkF BbF CHR BAA PYR FLA ANT PHN DBT FLU ACE ACY 0 BPH 5 NPH percent total 20 Kitimat PAH bioavailability estimates from stomach contents (PAHs in benthic prey) Puget Sound juvenile salmon flatfish (English sole) Kitimat 15,000 ng/g 1500 ng/g 8000 ng/g 2000 ng/g California halibut larval development and metamorphosis Text Los Pensaquitos Lagoon, San Diego Experimental design 25 per tank, 3 tanks per dose, 3 ∑PAH levels + control 2009 survival at 47 days 15 per tank, 6 tanks per dose, 4 ∑PAH levels + control 2011 harvest weekly to 42 days Experimental design 2009, clean and hot sediments mixed by hand in exposure tanks, allowed to settle 2011, clean and hot sediment homogenized as mud with a paint mixer, then distributed to tanks 2002 Hospital Beach ʖ3$+ SSE N0 N1 N2 N3 AY AE F0 D0 P0 MP1 A0 RE FL0 PY0 BAA C0 BBF BKF BAP BEP PER IDP DBA BZP 4500 4000 3500 3000 2500 2000 1500 1000 500 0 2010 Hospital Beach K100 ʖ3$+ N0 N1 N2 N3 BP AY AE F0 D0 P0 MP1 A0 RE FL0 PY0 BAA C0 BBF BKF BAP BEP PER IDP DBA BZP 1600 1400 1200 1000 800 600 400 200 0 Verification of PAHs in sediment dilutions 1600 1400 1200 1000 800 600 400 200 0 1600 1400 1200 1000 800 600 400 200 0 U100 ʖ3$+ K5-U95 ʖ3$+ K20-U80 ʖ3$+ 1600 1400 1200 1000 800 600 400 200 0 1600 1400 1200 1000 800 600 400 200 0 K50-U50 ʖ3$+ K100 ʖ3$+ N0 N1 N2 N3 BP AY AE F0 D0 P0 MP1 A0 RE FL0 PY0 BAA C0 BBF BKF BAP BEP PER IDP DBA BZP 1600 1400 1200 1000 800 600 400 200 0 Stage E, ~30 dph early juvenile, ~87 dph (42 days on sed) Stage G, ~42 dph (7 days on sed) 2.7 cm 0.9 cm Survival on Kitimat sediment at 47 days (2009) 6 survivors (mean ± SE) 5 0.35 4 3 0.12 0.06 2 1 0 clean 0.6 ppm 3 ppm ANOVA effect of treatment p = 0.1 Dunnettʼs post-hoc 15 ppm Mortality 2011 Growth on Kitimat sediment (2011) 30 12.3 ppm 5.4 ppm 2.6 ppm 0.7 ppm clean length (mm) 25 20 15 10 5 0 7 14 21 28 35 exposure duration (days) 42 49 Feeding Feeding An enriched aquaculture diet optimized for maximal fish production Feeding An enriched aquaculture diet optimized for maximal fish production Phytoplankton-enriched rotifers Feeding An enriched aquaculture diet optimized for maximal fish production Phytoplankton-enriched rotifers Phytoplankton-enriched Artemia Feeding An enriched aquaculture diet optimized for maximal fish production Phytoplankton-enriched rotifers Phytoplankton-enriched Artemia minced krill Feeding An enriched aquaculture diet optimized for maximal fish production Phytoplankton-enriched rotifers Phytoplankton-enriched Artemia minced krill Hand fed little baby pigs benzo[a]pyrene 72 hpf 48 hpf Distinct cardiac effects of BkF heart: endothelium (fli1GFP) CYP1A liver bud
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