Mycotoxins AOF MS-PREP® Product Code: P115 / P115B Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2, ochratoxin A, fumonisins B1 and B2 in a wide range of commodities in conjunction with LC-MS/MS. General Aflatoxins, ochratoxin A and fumonisins in the sample are extracted, filtered, diluted and passed slowly through the AOF MS-PREP® column where binding takes place between the antibodies and the toxins. The column is then washed to remove any extraneous unbound pigments. The purified aflatoxins, ochratoxin and fumonisins are released from the column using 100 % methanol prior to injection onto LC-MS/MS. The toxins are then quantified in one LC-MS/MS run. Specifications Format: 10 or 50 wide format immunoaffinity columns. Sample Preparation: Extraction, filtration and dilution. Extraction: Methanol. Benefits Sensitive: Low % CV, excellent recoveries and limit of detection complies with legislation. Rapid: Only one extraction and a single column clean-up required taking as little as 20 minutes prior to detection by LC-MS/MS. Adaptable: Specific: Reduced Run Time: 02/2014 Cost Effective: R-Biopharm Rhône Ltd Block 10 Todd Campus West of Scotland Science Park Acre Road, Glasgow G20 0XA www.r-biopharm.com The columns are suitable for testing a wide range of commodities. Uses monoclonal antibodies to selectively isolate and concentrate aflatoxins, ochratoxin and fumonisin. Simultaneous detection of aflatoxins, ochratoxin and fumonisins in one LC-MS/MS run. No need for three separate extractions and clean-up, saving time and money. Improved Reduces interference and therefore the need to use matrix matched Clean-up:standards. Robust: Shelf life of 18 months at 2 - 8 °C (or 12 months at 21 - 25 °C).
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