Table S2. Morpholino Sequences, Related to Experimental Procedures Gene name TIF1γ Cdc73 cdc73con Rtf1 3 Ctr9 4 Ctr9con Paf1 Spt5 Spt16 Ssrp1a Cdk9 CPSF2 CPSF2 CPSF3 CstF3 Rad6A Rad6B 1 Accession # NM_001002871 NM_200348 NM_001083577 NM_001083583 NM_001024453 NM_131673 NM_001097584 NM_212802 NM_212591 NM_001002384 NM_001002384 NM_001003836 NM_214719 NM_199719 NM_201273 MO target1 atg atg atgmismatch atg atg atgmismatch e1/i1 e7/i7 atg atg e3/i3 e1/i1 e2/i2 atg atg atg atg Mo sequence CCGTACAATCTTGGCCTTTGTTGTC CGCCATGATCGCGCCTCCTCATTAC CGCCATcATgGCGCgTCgTgATTAC Working conc. 2 100uM 100uM 100uM CTTTCCGTTTCTTTACATTCACCAT GATTTCAATGGATCCCCGAGACATG GATTTgAATGcATCgCCcAcACATG 100uM 100uM 100uM TATTATGGTTTATTCCTCTCACCGG AGATTTTCTTCATACTTGACCCCAG TCCAGACTCACCGCCATAATATCAC ACTCCAGAGTGTCTCCCATCTTTTG GGTGCATTTTCTTACCCCTTCTTTC GATCATTTCTCTCACCTTTCAGGTT CAGATTTCTAGACTCACATCTCCCT CTTTACGTTTGGTAGCCATGTTTGT TGCAATCCGAGTAAAGGAACGTCGA ACTTGGAGAACTGAGACGTAAACAT GGGTGGACATCTTTAACTACACTTA 125uM 100uM 300uM 300uM 200uM 500uM 500uM 500uM 350uM 150uM 150uM MOs targeting to the 5’UTR or translation start site are indicated as ’atg’. MOs targeting to the donor site of an exon/intron boundary are indicated as ‘e/i’ with the number of the exon/intron. 2 The working concentration of each MO was injected into 1-4 cell stage embryos at the volume of 1nl. 3, 4 The references of these MOs are: Rtf1 and Ctr9 (Akanuma et al., 2007).
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