PATHOGENIC SYSTEM AST
Identification and Antimicrobial Susceptibility Testing of the main
pathogenic microorganisms directly from Clinical Specimens
✹
•
4
HAE
5
NES
10
ESC
11
IND
12
KES
13
VP
18
CLA
19
AUG
20
AMS
26
PEF
27
LEV
28
OFX
9
PRO
17
AZM
25
CXM
*
2
SGA
6
STG
7
STR
8
PSE
14
CAN
15
CN
16
NET
21
TZP
22
CRO
23
CFP
24
CAZ
29
RD
30
MRP
31
SXT
32
Growth
*
*
Pharyngeal swab, Cutaneous swab,
Auricular swab, Ophthalmic swab,
Expectorate, Exudates and other clinical specimens.
✹
PATHOGENIC SYSTEM AST
Date ........................................
•
3
STA
4
HAE
5
NES
10
ESC
11
IND
12
KES
13
VP
18
CLA
19
AUG
20
AMS
26
PEF
27
LEV
28
OFX
9
PRO
17
AZM
25
CXM
*
2
SGA
6
STG
7
STR
8
PSE
14
CAN
15
CN
16
NET
21
TZP
22
CRO
23
CFP
24
CAZ
29
RD
30
MRP
31
SXT
32
Growth
*
*
1
PYR
PATHOGENIC SYSTEM AST
Specimen ...........................
Direct microbial identification
Streptococcus pyogenes (group A), Staphylococcus aureus,
Haemophylus spp., Neisseria spp., Escherichia coli,
Streptococcus agalactiae (group B),
Klebsiella spp., Enterobacter spp., Serratia spp.,
Proteus spp., Providencia spp., Pseudomonas spp.,
Enterococcus spp. (group D), Candida spp.
Specimen ...........................
*
✹
Antimicrobial Susceptibility Testing
Gentamicin, Netilmicin, Azithromycin, Clarithromycin,
Amoxicillin-Clavulanic Acid, Ampicillin-Sulbactam,
Piperacillin-Tazobactam, Ceftriaxone, Cefoperazone,
Ceftazidime, Cefuroxime, Pefloxacin, Levofloxacin,
Ofloxacin, Rifampicin, Meropenem, Co-trimoxazole.
Date ........................................
✹ Benefits
*
•
3
STA
4
HAE
5
NES
10
ESC
11
IND
12
KES
13
VP
18
CLA
19
AUG
20
AMS
26
PEF
27
LEV
28
OFX
2
SGA
9
PRO
17
AZM
25
CXM
*
1
PYR
6
STG
7
STR
8
PSE
14
CAN
15
CN
16
NET
21
TZP
22
CRO
23
CFP
24
CAZ
29
RD
30
MRP
31
SXT
32
Growth
*
*
Liofilchem® and the Liofilchem company logo are registered trademarks of LIOFILCHEM s.r.l.
*
3
STA
1
PYR
Clinical Specimens
✓ Complete diagnosis
✓ Save time and labor with direct testing
✓ Results in 18-24 hours
✓ Confirmation with latex agglutination tests
✓ Clear and well defined color changes
✓ Easy interpretation
PATHOGENIC SYSTEM AST
Specimen ...........................
Date ........................................
www.liofilchem.net
PATHOGENIC SYSTEM AST
RESULTS
TEST PROCEDURE
1
Identification
•
1
PYR
•
*
•
3
STA
3
STA
2
SGA
1* PYR test
2• confirm by latex test
Staphylococcus
aureus
4
HAE
3• confirm by
latex test
4
HAE
Neisseria spp.
5* Oxidase test
*
7
STR
7
STR
9
PRO
9
PRO
•
5
NES
•
6
STG
6
STG
Enterococcus
spp.
8
PSE
8
PSE
Haemophylus
spp.
- confirm by
culture medium
Streptococcus
agalactiae
6• confirm by
•Dip the clinical specimen
into a tube of nutrient broth and
incubate at 36±1°C
for 2-3 hours.
latex test
•Transfer 0.2 mL of culture broth
into the vial of Physiological
Solution (Suspension A).
•Transfer 0.01 mL of culture
broth into the vial of Inoculum
Broth 2 (Suspension B).
Pseudomonas
spp.
3
4
Proteus spp.
Providencia spp.
Escherichia coli
11
IND
*
13
VP
10
ESC
12
KES
11
IND
13
VP
*
12
KES
*
10
ESC
*
Liofilchem® and the Liofilchem company logo are registered trademarks of LIOFILCHEM s.r.l.
*
2
SGA
Streptococcus pyogenes
•
1
PYR
2
11* Positive Indole test
Klebsiella spp.
Enterobacter spp.
Serratia spp.
13* Positive VP test
•Add a Pyr Disc into the well
1-PYR.
•Add 0.2 mL of the Suspension B
into each well from 15 to 32.
(Antimicrobial Susceptibility Testing)
•Add 0.2 mL of the Suspension A
into each well from 1 to 14.
(Identification)
•Incubate at 36±1° C for 18-24 h.
Candida spp.
14
CAN
14
CAN
- confirm at the microscope
5
Antimicrobial Susceptibility Testing
Sensitivity
26
PEF
26
PEF
6
Resistance
27
LEV
27
LEV
DESCRIPTION
PACKAGING
REF.
PATHOGENIC SYSTEM AST
20 tests
71681
OXIDASE TEST STICK
30 tests
88029
STREPTO A LATEX KIT
50 tests
96154
STREPTO B LATEX KIT
50 tests
96153
STAPH LATEX KIT
50 tests
96017
•Confirm the presence of:
-Streptococcus pyogenes (group A)
-Staphylococcus aureus
-Haemophylus spp.
-Neisseria spp.
-Streptococcus agalactiae (group B)
as by Instruction For Use.
64026 Roseto degli Abruzzi, Italy
Tel. : +39 0858930745
Fax : +39 0858930330
www.liofilchem.net
•Confirm Candida spp. by taking
a drop from the positive
well 14-CAN and watch for spores
at the microscope (400x).
•Note down the test results on
the form provided in the kit, or read
and interpret by automatic readers.
ref. 6553466 - Rev. 1/28.02.2014
© Liofilchem 2014 Printed in Italy

Adrian Thomas - European Medicines Agency