SAMPLE PREPARATION Chrom Tech Filtration

S A M P L E P R E P A R A T I O N Chrom Tech Filtration
Centrifuge Filter Tubes
Micro, Midi, and Maxi Spin centrifuge filters are very useful for small volume sample filtration.
Designed for use in centrifuges, the filter units consist of a sample chamber and receiver tube.
This tube has a marking area and is graduated. A cap serves the dual purpose of capping the
sample chamber during the centrifugation and the receiver tube after the sample chamber has
been removed. Each package is imprinted to identify the pore size and membrane type.
Micro-Spin Filter Tubes
Quickly and easily clarify small sample volumes with Chrom Tech's Micro-Spin®
centrifuge filter tubes. These devices fit in any standard microcentrifuge, and allow
you to process multiple samples simultaneously. Less than 5µL hold-up volume
provides near quantitative volumetric recovery. Maximum G-force: 10,000xG.
Micro-Spin centrifuge filter tubes now include glass prefilters.
FE AT U RE S


For up to 850 µL sample volumes
Polypropylene housings
Midi-Spin Filter Tubes
Midi-Spin® filter tubes consist of a polypropylene insert that houses a filter
membrane and a tapered 5.0ml filtrate tube. Inserts can accommodate sample
volumes up to 2ml. Maximum G-force: 5,000xG.
FE AT U RE S


For up to 5 mL sample volumes
Polypropylene housings
Maxi-Spin Filter Tubes
Maxi-Spin® centrifuge filter tubes remove particulates from larger sample sizes
with any centrifuge that holds 50 mL conical tubes. They should only be used with
a fixed-angle rotor centrifuge to prevent premature clogging of the membrane.
Maximum operating temperature: 50º C. Maximum G-force: 2,500xG.
FE AT U RE S



For up to 25 mL sample volumes
Polypropylene housings
Used with fixed angle rotor centrifuge
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Chrom Tech Filtration S A M P L E P R E P A R A T I O N
Centrifuge Filter Tubes (continued)
M E M BR A N E
PO R E SIZ E
MICRO 100/pk
MID I 25/pk
MAXI 50/pk
Cellulose Acetate
0.2 µm
CTF-CA020-01
CTF-CA020-02
CTF-CA020-03
0.45 µm
CTF-CA045-01
CTF-CA045-02
CTF-CA045-03
0.2 µm
CTF-NC020-01
CTF-NC020-02
CTF-NC020-03
0.45 µm
CTF-NC045-01
CTF-NC045-02
CTF-NC045-03
0.2 µm
CTF-NY020-01
CTF-NY020-02
CTF-NY020-03
0.45 µm
CTF-NY045-01
CTF-NY045-02
CTF-NY045-03
0.2 µm
CTF-PS020-01
CTF-PS020-02
CTF-PS020-03
0.45 µm
CTF-PS045-01
CTF-PS045-02
CTF-PS045-03
0.2 µm
CTF-PP020-01
CTF-PP020-02
CTF-PP020-03
0.45 µm
CTF-PP045-01
CTF-PP045-02
CTF-PP045-03
0.2 µm
CTF-PT020-01
CTF-PT020-02
CTF-PT020-03
0.45 µm
CTF-PT045-01
CTF-PT045-02
CTF-PT045-03
0.2 µm
CTF-PV020-01
CTF-PV020-02
CTF-PV020-03
0.45 µm
CTF-PV045-01
CTF-PV045-02
CTF-PV045-03
0.2 µm
CTF-RC020-01
CTF-RC020-02
CTF-RC020-03
0.45 µm
CTF-RC045-01
CTF-RC045-02
CTF-RC045-03
NC (Nitrocellose)
Nylon
Polyethersulfone
Polypropylene
PTFE (polytetrafluoroethylene)
PVDF (polyvinylidene diffuoride)
Regenerated Cellulose
Forensic Spin Tubes
Our kit includes a 800 µL filter basket and a 2.0 mL microcentrifuge receiver tube
with attached cap. The 1.4 mm mesh acts as a sieve to retain cloth samples and
pass fluids to the receiver tube.
FE AT U RE S



800 µL capacity
Extracts fluids from cloth samples
Polypropylene housing
DE SCRI P T I O N
QTY
PART N O
Forensic Spin Tube
100
CTF-FS000-01
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S A M P L E P R E P A R A T I O N Chrom Tech Filtration
Membrane Filters
M E M BR A N E
PORE
SIZE
13 mm
100/pk
2 5 mm
100/pk
47 mm
100/pk
90 mm
25/pk
142 m m
25/pk
Cellulose
Acetate
0.2 µm
CTF-CA020-13
CTF-CA020-25
CTF-CA020-47
CTF-CA020-90
CTF-CA020-142
0.45 µm
CTF-CA045-13
CTF-CA045-25
CTF-CA045-47
CTF-CA045-90
CTF-CA045-142
Glass Fiber
0.7 µm
CTF-GF070-13
CTF-GF070-25
CTF-GF070-47
CTF-GF070-90
N/A
1.0 µm
CTF-GF100-13
CTF-GF100-25
CTF-GF100-47
CTF-GF100-90
N/A
3.1 µm
CTF-GF310-13
CTF-GF310-25
CTF-GF310-47
CTF-GF310-90
N/A
5.0 µm
CTF-GF500-13
CTF-GF500-25
CTF-GF500-47
CTF-GF500-90
N/A
0.2 µm
CTF-NC020-13
CTF-NC020-25
CTF-NC020-47
CTF-NC020-90
CTF-NC020-142
0.45 µm
CTF-NC045-13
CTF-NC045-25
CTF-NC045-47
CTF-NC045-90
CTF-NC045-142
0.2 µm
CTF-NY020-13
CTF-NY020-25
CTF-NY020-47
CTF-NY020-90
CTF-NY020-142
0.45 µm
CTF-NY045-13
CTF-NY045-25
CTF-NY045-47
CTF-NY045-90
CTF-NY045-142
0.2 µm
CTF-PS020-13
CTF-PS020-25
CTF-PS020-47
CTF-PS020-90
CTF-PS020-142
0.45 µm
CTF-PS045-13
CTF-PS045-25
CTF-PS045-47
CTF-PS045-90
CTF-PS045-142
0.2 µm
CTF-PT020-13
CTF-PT020-25
CTF-PT020-47
CTF-PT020-90
CTF-PT020-142
0.45 µm
CTF-PT045-13
CTF-PT045-25
CTF-PT045-47
CTF-PT045-90
CTF-PT045-142
0.2 µm
CTF-PV020-13
CTF-PV020-25
CTF-PV020-47
CTF-PV020-90
CTF-PV020-142
0.45 µm
CTF-PV045-13
CTF-PV045-25
CTF-PV045-47
CTF-PV045-90
CTF-PV045-142
NC
Nylon
PS
PTFE
PVDF
Membrane Selection Guide
RC
Nylon
Regenerated Cellulose (RC) is a hydrophilic, solvent resistant, low protein binding membrane. Ideal for removing
particulates from HPLC samples prior to injection. Compatible with all HPLC solvents. Use for particle removal
and degassing of solvents. RC membranes are also compatible with aqueous solutions in the 3 to 12 pH range.
Extractables with water are less than 1%. RC membranes exhibit low, non-specific adsorption — good for biological
sample filtration where maximum protein recovery is important. When used with a glass prefilter, RC is ideal for tissue
culture media filtration, as well as general biological sample filtration.
Commonly used for general laboratory filtration and filtration of HPLC samples prior to injection. Nylon is solvent
resistant and exhibits low extractables. Nylon binds protein and should not be used when protein recovery is important.
PTFE
PTFE (polytetrafluoroethylene) is a hydrophobic and chemically resistant to all solvents, acids and bases. Does not
impart extractables to the filtrate. PTFE blocks water vapor, making it ideal for transducer protectors. Also ideal for
filtering and degassing chromatography solvents.
PVDF
PVDF (polyvinylidene diflouride) is a hydrophilic, solvent resistant membrane that exhibits low levels of UV adsorbing
extractables. Use for HPLC sample filtration and general biological filtration. PVDF is a low protein binding membrane.
Polypropylene
Polypropylene is a hydrophilic membrane that exhibits a wide range of chemical compatibility to organic solvents.
A good choice for HPLC sample filtration when performing chromatography protein analysis. High solvent resistant.
Low non-specific adsorbing membrane for maximum protein recovery in critical analysis. Also well suited for
biological sample filtration.
Glass
Microfiber
Glass Microfiber membranes are commonly used as pre-filters to remove large particulates and extend the load
capacity of the membrane.
Cellulose
Acetate
Cellulose Acetate is a very low protein binding membrane, ideal for aqueous based samples. CA membranes are an
excellent choice when maximum protein recovery in filtrate is critical. Laboratory studies show the CA membranes
bind less protein that PVDF. When used with a glass pre-filter, CA is ideal for tissue culture media filtration and
sensitive biological samples. The pre-filter increases yield.
NC
NC (Nitrocellulose - Mixed Esters (ME) - unsupported filters are hydrophilic and composed of a mixture of inert
cellulose nitrate and cellulose acetate polymers. The uniform microporous structure of these filters provides the
fastest flow rates and highest throughputs available in a membrane filter. Because they are biologically inert, NC
filters are ideal for a wide range of clarification, sterilization, and analytical applications.
PS
PS (Polyethersulfone) is the filter of choice for tissue culture work. It has very low extractables. Low binding of
protein and nucleic acids. It has relatively broad chemical resistance except to aggressive solvents and it has good
to excellent flow rates.
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Chrom Tech Filtration S A M P L E P R E P A R A T I O N
CA (Cellulose Acetate)
CA (Cellulose Acetate) membrane filters are composed of pure cellulose acetate modified to offer
researchers the lowest binding filters available. Due to the extremely low binding characteristics,
these filters provide higher throughputs than competitive offerings and reduce filter changes when
filtering proteinaceous solutions. Because of their unique strength and extremely low binding
characteristics, CA (Cellulose Acetate) filters are ideal for protein and enzyme filtrations, tissue
culture media sterilization, cold sterilization, biological fluid filtration and other filtration applications
where maximum recovery of proteins is critical.
CA (Cellulose Acetate) membranes are manufactured using a unique impregnation process that is
internally supported by aninert polyester web eliminates cracking, tearing, breaking and distortion
when handled or creased. Each filter has unequalled dimensional stability after autoclaving or
steam sterilizing and is completely unaffected by temperatures up to 135°C (275°F). The exclusive
impregnation process results in an acetate filter which has a burst strength of 130 PSI, uniform pore
size and consistent flow rates for reliable performance.
F EATU RE S





Lowest binding material available
Hydrophilic
High throughput
Strength and dimension stability
Uniform pore structure
A PPLI C ATI ON S



Protein and enzyme filtration, sterilization
Biological fluid filtration sterilization
Tissue culture media sterilization
Product Characteristics
PROD U C T C H A R A C T E R I S T I CS
Sterilization
Gamma Irradiation or Ethylene Oxide (EtO)
USP Class VI testing
Passed
Thickness
65 – 110 µm
BSA Protein Binding
3.8 µg/cm2
Maximum Operating Temperature
135 ºC (274 ºF)
Sealing Compatibility
Ultrasonics, Heat, Radio Frequency and Insert Molding
Pore Size Range
0.1 – 20 µm
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S A M P L E P R E P A R A T I O N Chrom Tech Filtration
Nitrocellulose
Nitrocellulose - Mixed Esters (ME) - unsupported filters are composed of a mixture of inert cellulose
nitrate and cellulose acetate polymers. The uniform microporous structure of these filters provides
the fastest flow rates and highest throughputs available in a membrane filter. Because they are
biologically inert, Nitrocellulose filters are ideal for a wide range of clarification, sterilization, and
analytical applications such as: microbiological analysis, clarification or sterilization of aqueous
solutions, industrial hygiene applications, silt density index (SDI - 0.45μm, 47mm) and particulatematter analysis. For gravimetric analysis using ashing techniques, Nitrocellulose filters yield a
residue of less than 0.045% of their initial weight.They are hydrophilic with a noncytotoxic wetting
agent extractable level of less than 4% of their weight.
Nitrocellulose filters are available in pore sizes ranging from 0.1μm to 15.0μm. Standard disc
diameters are available from 13 mm to 293 mm. These filters are autoclavable at 121°C (250°F)
for 20 minutes.
F EATURE S





High binding capacity
Consistent high flow rate for faster filtration
Uniform pore structure for selectivity
Hydrophilic, inert cellulose nitrate
Manufactured thickness within 10 microns
A PPLI C ATI ONS






Aqueous filtration
Microbiological analysis
Sterility testing
Gravimetric analysis with ashing technique
Particulate analysis
Black — food and beverage
Product Characteristics
PROD U C T C H A R A C T ER I S T I CS
Sterilization
Gamma Irradiation or Ethylene Oxide (EtO)
USP Class VI testing
Passed
Thickness
Approx. 6 mil (150 µm) +/- 10 µm
Extractables
<4%
BSA Protein Binding
Approx. 160 µg/cm2 (depending on pore size)
Maximum Operating Temperature
180 ºC (356 ºF)
Sealing Compatibility
Ultrasonics, Heat, Radio Frequency and Insert Molding
Wicking Rate
Time required for an aqueous solution to travel 4 cm.
5.0 µm – 200 sec. 8.0 µm – 120 sec. 15.0 µm – 70 sec.
Pore Size Range
0.1 – 15 µm
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Chrom Tech Filtration S A M P L E P R E P A R A T I O N
Nylon
Nylon membrane filters are fast becoming the "standard" filter material due to their wide chemical
compatibility range and naturally hydrophilic characteristics. Nylon filters can be used to filter all
aqueous and the majority of solvent solutions.
Nylon filters are manufactured using a unique impregnation process that eliminates cracking,
tearing, breaking and distortion when handled or creased. Each membrane filter has unequalled
dimensional stability even after autoclaving and is completely unaffected by temperatures up to
180°C (356°F) or steam sterilizing. The exclusive impregnation process results in a nylon filter with
uniform pore sizes and consistent flow rates for continuously reliable performance.
Since Nylon filters are naturally hydrophilic, wetting agents are not used during manufacturing,
resulting in a very low level of extractables. Through gravimetric analysis, it has been found that
Nylon filters have an extractable level of less than 0.0015 mg/cm² making them ideally suited for
HPLC solvent filtration, HPLC sample preparation, mobile-phase filtration and critical aqueous
applications. The flow rates of nylon membrane filters are approximately equal to those of standard
cellulosic membranes. They have a high nucleic acid binding capacity of 350 μg/cm² and Bovine
Serum Albumin (BSA) protein binding of 120 μg/cm² compared to 80-100 μg/cm² for most cellulosic
membranes under the same BSA binding conditions.
F EATURE S




Naturally hydrophilic
Wide chemical compatibility range
Strength and dimensional stability
Extremely low extractables
A PPLI C ATI ONS


Sterilization, clarification of aqueous and
organic solvent solutions
HPLC sample preparation
Product Characteristics
PROD U C T C H A R A C T ER I S T I CS
Sterilization
Gamma Irradiation or Ethylene Oxide (EtO)
USP Class VI testing
Passed
Thickness
65 – 125 µm
Extractables
<0.2% (<0.0015 mg/cm2)
BSA Protein Binding
Approx. 120 µg/cm2
Maximum Operating Temperature
180 ºC (356 ºF)
Sealing Compatibility
Ultrasonics, Heat, Radio Frequency and Insert Molding
Pore Size Range
0.1 – 20 µm
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S A M P L E P R E P A R A T I O N Chrom Tech Filtration
PES (Polyethersulfone)
PES (polyethersulfone) membrane filters are composed of polyethersulfone and manufactured
within a tight specification range to ensure a uniform pore structure for low binding applications
where reliable fast flow rates and high throughputs are required. PES (polyethersulfone) filters are
hydrophilic and can withstand high temperatures 135°C (275°F) without membrane degradation as
required for autoclaving applications. They may provide better strength than cellulosic-type membranes.
PES (polyethersulfone) filters are inert which provide extremely low extractable levels in critical
sterilization applications such as protein and enzyme filtrations, tissue culture media sterilization,
cold sterilization, biological fluid filtration and other sterilization applications where maximum
throughputs and fast flow rates are the critical factors.
F EATURE S






Very low protein binding
Flast flow rates
Low extractables
Wide chemical compatibility range
Strength and dimensional stability
Autoclavable
A PPLI C ATI ONS





Protein and enzyme filtration sterilization
Biological fluid filtration sterilization
Tissue culture media sterilization
Pharmaceutical sterilization filtration
Environmental water studies
Product Characteristics
PROD U C T C H A R A C T ER I S T I CS
Sterilization
Gamma Irradiation, Ethylene Oxide (EtO), Autoclaving or Live Stream
USP Class VI testing
Passed
Thickness
110 – 150 µm
Extractables
2% (<0.015 mg/cm2)
BSA Protein Binding
<20 µg/cm2
Maximum Operating Temperature
130 ºC (266 ºF)
Sealing Compatibility
Ultrasonics, Heat, Radio Frequency and Insert Molding
Pore Size Range
0.03 – 20.0 µm
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Polypropylene
Polypropylene membrane filters are composed of pure polypropylene with absolute pore size
ratings. These filters offer broad chemical compatibility allowing its use with aqueous and organic
solvent samples. The polypropylene filter has extremely low extractable levels designed to
provide accurate, consistent analysis results for sensitive ion chromatography applications while
prolonging column life. Polypropylene filter is the preferred filter membrane for HPLC applications
where the detection levels are below 230 nm. The filters also exhibit negligible protein binding
which, is essential for maximum sample recovery of critical, small volume protein samples. Filters
are available in 0.1, 0.22 and 0.45-micron pore sizes in a wide range of disc diameters.
F EATU RE S



Broad chemical compatibility
Hydrophobic
HPLC applications – detection level < 230 nm
A PPLI C ATI ON S




Aqueous and organic solvent filtration
HPLC sample preparation requiring low
detection levels
Ion chromatography
Total digest for heavy metals
PTFE Unlaminated
PTFE polytetrafluoroethylene membrane consists of a pure PTFE laminated to a polypropylene
support for improved durability and easy handling. These filters are chemically compatible with
strong acids and most aggressive solvents such as alcohols. Laminated PTFE filters can also
be used to filter aqueous solutions when prewetted with methanol. They are autoclavable up to
130°C (260°F) 175 μm thick.
F EATU RE S



Naturally hydrophobic
Compatible with strong acids and
aggressive solutions
Improved durability and handling
A PPLI C ATI ON S




Filtration of strong acids and
aggressive solutions
Venting applications
Phase separations
Aerosol samplings
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