Curriculum Vitae Mirjam van der Burg, PhD

CV of M. van der Burg
May 2014
Curriculum Vitae
Mirjam van der Burg, PhD
Working address and contact details
Department of Immunology
Erasmus MC, University Medical Center Rotterdam
Wytemaweg 80
3015 CN ROTTERDAM
The Netherlands
tel. (31)10.7043015
fax (31)10.7044731
e-mail: [email protected]
Personal information
Date of birth: 6th of July 1974
Place of birth: Rotterdam, The Netherlands
Academic education and degrees
Sept. 1992 – March 1997
MSc Biochemistry & Molecular biology at Leiden University
Cum laude
May 1997-December 2001
PhD at Dept. of Immunology, Erasmus University Rotterdam, NL
Thesis: “Recombination processes during human B-cell
differentiation”
Promotor: prof. dr. J.J.M. van Dongen; co-promotor: dr. A.W.
Langerak
Thesis defence: 19th of June 2002
Appointments since completing PhD
2002-2007
Fixed term at Dept. of Immunology, Erasmus MC as Post Doc with supervision of 1
PhD student and 2 technicians (1.6 fte) and 1-2 students of Medical University or
students from HLO
2007- present Tenured term position at Dept. of Immunology, Erasmus MC, since 2009 as Assistant
Professor. Supervision of 2 PhD students, 4 technicians (3.0 fte) and 1-2 students of
Medical University or Master students and regular international visiting scientists.
Description of current research
After the PhD, Mirjam van der Burg started her own research group on Primary Immunodeficiencies
(PID) at the Department of Immunology, Erasmus MC. The current Workgroup PID consists of 1
post-doc, 1 pediatric immunologist, 2 PhD students, 4 technicians (3.0 fte) and 1-2 students of Medical
School or Master students and regular international visiting scientists. The two research lines are:
1. V(D)J recombination and its defects in severe combined immunodeficiency
2. Immunopathological causes and consequences of primary antibody deficiencies (PAD)
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CV of M. van der Burg
May 2014
The main scientific contributions over the past five years were the identification of four new candidate
genes for PID, i.e. genes that can be mutated in PID. Genetic defects in two of the new candidate
genes were related to severe combined immunodeficiency (LIG4 and DNA-PKcs) and link to research
line no. 1 and two genes were related to primary antibody deficiencies (CD19 and CD81) which link
to research line no. 2. With identification of mutations in CD19 and CD81, a new PAD category of
CD19-complex deficiencies or BCR-signaling deficiencies was defined. In addition, the PID group
studies immunological processes that are disturbed in PID as disease model for monogenetic defects.
The strength of the research group is the combination of basic research on immunological processes
and PID. For PID research, diagnostics and patient care in the Erasmus MC, an Immunodeficiency
Center has been initiated by the Depts. of Immunology (Dr. M. van der Burg), Pediatrics (Dr. G.
Driessen) and Internal Medicine (Prof.dr. P.M. van Hagen), which has been qualified as a Jeffrey
Modell Foundation center of Excellence of PID April 2010 based on outstanding PID research and
patient care.
International activities
European consortia
- Leader of EuroChimerism Workpackage DNA isolation from low cell numbers (part of project
QLRT-2001-01485) 2002 - 2006
- Leader of EuroFlow PID Workpackage (consortium of 7 European PID laboratories) for
development of standardized flow cytometric diagnostics for PID (part of EU-LSHB-CT-2006018708) 2011 - present
International PID workgroups for guidelines and disease definition
- Member of the European Society of Immunodeficiencies (ESID) and member of the Working
party Genetics.
- Participant of international workgroup “International Update on definition of CVID” chaired by
Prof. K. Warnatz, Freiburg, Germany.
- Participant of international workgroup “Consensus meeting for the diagnosis of combined
immunodeficiencies” chaired by Prof. S. Ehl, Freiburg, Germany and Capucine Picard, Paris,
France.
- Participant and co-author of the Clinical and Laboratory and Standards Institute (CLSI) document
I/LA36, Newborn Bloodspot Screening for Severe Combined Immunodeficiency (SCID) by
Measurement of T-cell receptor excision circles (TREC). Chairman, dr. W.J. Hannon, Atlanta,
GA, USA
National and International memberships
-
Member of the national Workgroup of Immunodeficiencies (WID) and coordinator of the genetic
testing for PID candidate genes in the Netherlands
Member of “Jong GR network” (national network of Junior Health Organization)
Member of the Dutch Society for Immunology (NVVI)
Member of the European Society of Immunodeficiencies (ESID)
Member of the European Academy of Allergy and Clinical Immunology (EAACI)
Reviewer
Reviewer for Blood, Journal of Allergy and Clinical Immunology, Molecular Immunology, Cytometry
Part B, Haematologica, Frontiers in Immunology, Journal of Clinical Immunology, Nature reviews in
Immunology.
Associate-editor
Associate-editor of Frontier in Immunology
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CV of M. van der Burg
May 2014
Teaching activities
-
Clinical immunology for medical students, case discussions (2000-present)
Elective courses on Gene Therapy (2006-2008, 2011- present)
Faculty member of the Master Molecular Medicine (2009-present) Lectures on PID and
Critical Research Topics
Master Infection and Immunity: PID lectures, Molecular Technique Practicals (2009present)
Training for Medical Immunologists on diagnostics of primary Immunodeficiencies (2005present)
Teacher/Faculty member at European Society of Immunodeficiencies (ESID) Summer
School (2011, 2013)
Coordinator “tweedejaarskeuzeonderwijs PID”for medical students (2013 - )
Coordinator week “antibody deficiencies” in minor Ever thought of doing research (2013-)
Co-promotorships/PhD supervision
-
Menno C. van Zelm – B-cell development and primary antiboyd deficiencies – 20 June 2007 cum
laude
Gertjan J.A. Driessen – Immunobiology of Primary Antibody Deficiencies: towards a new
classification – 16 October 2013
Hanna IJspeert – Clinical and immunological diversity of recombination defects – 19 March 2014
Member of PhD thesis defense committee
-
Annick van de Ven, UMC Utrecht, November 2011
Scholarships and prizes
-
-
Best publication award 2010 of the research school Molecular Medicine for the paper A DNAPKcs mutation in a radiosensitive T-B- SCID patient inhibits Artemis activation and
nonhomologous end-joining. Van der Burg et al. J Clin Invest. 2009;119:91-98.
NWO Aspasia Award for talented female scientist (2013)
Grant allocations
2005: ZonMW Veni grant 916.56.107 “V(D)J recombination under control”
Principle investigator, (€150K, 3 years)
2005: Project “Lokale Productiegebonden Component”: “Zorgvernieuwing voor primaire
immunodeficiënties door snellere en doelmatigere diagnostiek”
Co-investigator, (€219K, 3 years)
2006: Baxter research grant: “Identification of B-cell differentiation/maturation defects in common
variable immunodeficiency (CVID) as powerful tool for a new classification”
Principle investigator, (€50K, 2 years)
2008: SSWO project: “Scope on SCID: unraveling clinical and immunogenetic heterogeneity of
SCID” Principle investigator, (€220K, 4 years)
2012: Baxter research grant: “Primary Antibody Deficiencies: Early and accurate diagnosis of new
patients and identification of new genetic defects”
Principle investigator, (€25K, 1.5 years)
2012: ZonMW Vidi grant 016.126.323 “Immune Repertoire in the Picture”
Principle investigator (€800K, 5 years)
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CV of M. van der Burg
May 2014
2013: MultiPAD project Baxter
Principle investigator together with GJ Driessen (€100K, 3 years)
Courses
-
Cursus Managementvaardigheden (October 2000)
Cursus Leidinggeven (June2001)
Praktisch Project Mananagement (June 2003)
Cursus Probleem management (March 2005)
Cursus WOD art 9 (September 2005)
Cursus academisch Leidinggeven, including financial management (January-June 2007)
Female Career Development Program 9 (October 2012-October 2013)
International SCI publications
10 key publications
1.
IJspeert H, Driessen GJ, Moorhouse MJ, Hartwig NG, Wolska-Kusnierz B, Kalwak K, Pituch-Noworolska
A, Kondratenko I, van Montfrans JM, Mejstrikova E, Lankester AC, Langerak AW, van Gent DC, Stubbs
AP, van Dongen JJ, van der Burg M. Similar recombination-activating gene (RAG) mutations result in
similar immunobiological effects but in different clinical phenotypes. J Allergy Clin Immunol.
2014;133:1124-1133 e1121. IF:12.0
2. Castiello MC, Bosticardo M, Pala F, Catucci M, Chamberlain N, van Zelm MC, Driessen GJ, Pac M,
Bernatowska E, Scaramuzza S, Aiuti A, Sauer AV, Traggiai E, Meffre E, Villa A, van der Burg M.
Wiskott-Aldrich Syndrome protein deficiency perturbs the homeostasis of B-cell compartment in humans. J
Autoimmun. 2014;50:42-50. IF:8.1
3. IJspeert H, Warris A, van der Flier M, Reisli I, Keles S, Chishimba S, van Dongen JJ, van Gent DC, van
der Burg M. Clinical spectrum of LIG4 deficiency is broadened with severe dysmaturity, primordial
dwarfism, and neurological abnormalities. Hum Mutat. 2013;34:1611-1614. IF:5.2
4. Driessen GJ, Ijspeert H, Weemaes CM, Haraldsson A, Trip M, Warris A, van der Flier M, Wulffraat N,
Verhagen MM, Taylor MA, van Zelm MC, van Dongen JJ, van Deuren M, van der Burg M. Antibody
deficiency in patients with ataxia telangiectasia is caused by disturbed B- and T-cell homeostasis and
reduced immune repertoire diversity. J Allergy Clin Immunol. 2013;131:1367-1375 e1369. IF:12.0
5. Driessen GJ, van Zelm MC, van Hagen PM, Hartwig NG, Trip M, Warris A, de Vries E, Barendregt BH,
Pico I, Hop W, van Dongen JJ, van der Burg M. B-cell replication history and somatic hypermutation
status identify distinct pathophysiologic backgrounds in common variable immunodeficiency. Blood.
2011;118:6814-6823. IF:9.1
6. van der Burg M, Pac M, Berkowska MA, Goryluk-Kozakiewicz B, Wakulinska A, Dembowska-Baginska
B, Gregorek H, Barendregt BH, Krajewska-Walasek M, Bernatowska E, van Dongen JJ, Chrzanowska KH,
Langerak AW. Loss of juxtaposition of RAG-induced immunoglobulin DNA ends is implicated in the
precursor B-cell differentiation defect in NBS patients. Blood. 2010;115:4770-4777. IF:9.1
7. van Zelm MC, Smet J, Adams B, Mascart F, Schandene L, Janssen F, Ferster A, Kuo CC, Levy S, van
Dongen JJ, van der Burg M. CD81 gene defect in humans disrupts CD19 complex formation and leads to
antibody deficiency. J Clin Invest. 2010;120:1265-1274. IF:12.8
8. van der Burg M, Ijspeert H, Verkaik NS, Turul T, Wiegant WW, Morotomi-Yano K, Mari PO, Tezcan I,
Chen DJ, Zdzienicka MZ, van Dongen JJ, van Gent DC. A DNA-PKcs mutation in a radiosensitive T-BSCID patient inhibits Artemis activation and nonhomologous end-joining. J Clin Invest. 2009;119:91-98.
IF12.8
9. van der Burg M, van Veelen LR, Verkaik NS, Wiegant WW, Hartwig NG, Barendregt BH, Brugmans L,
Raams A, Jaspers NG, Zdzienicka MZ, van Dongen JJM, van Gent DC. A new type of radiosensitive T-BNK+ severe combined immunodeficiency caused by a LIG4 mutation. J Clin Invest. 2006;116:137-145.
IF12.8
10. van Zelm MC, Reisli I, Van der Burg M, Castano D, van Noesel CJ, van Tol MJ, Woellner C, Grimbacher
B, Patino PJ, van Dongen JJM, Franco JL. An antibody-deficiency syndrome due to mutations in the CD19
gene. N Engl J Med. 2006;354:1901-1912. IF:51.6
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H-index Web of Science: 26 (May 2014)
Of the 26 manuscripts were 7 manuscripts as first author and 3 as last author
Number of publications: 89
Number of citations: 2132
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CV of M. van der Burg
May 2014
Books, or contributions to books
1.
2.
De Villartay JP, Van der Burg M, Schwarz K, Villa A. V(D)J recombination defects. In: Ochs HD, Smith
CI, Puck J, eds. Primary immunodeficiency diseases: a molecular and genetic approach (ed 3rd): Oxford
University Press; 2014
van der Burg M, Weemaes CMR, Cunningham-Rundles C. Isotype Defects. In: Sullivan KE, Stiehm R,
eds. Stiehm textbook on Primary Immune Deficiencies: Elsevier; 2014:in press
Invited presentations to peer-reviewed, internationally established conferences and international
advanced schools
1. Radiation sensitivity and DNA repair defects: the present and the future & B-cell
immunophenotyping in PIDs, Clinical Immunology Society (CIS) Annual meeting, Baltimore,
USA (2014)
2. Novel insights in molecular biological causes of (severe) combined immune deficiencies. CeMM
Impromptu Series, Vienna, Austria (2014)
3. B-cell deficiencies from different angles; European Society of Immunodeficiency (ESID) Summer
School, Crete, Greece (2013)
4. Agammaglobulinemia and CSR deficiency; 2nd workshop on Diagnostics of Immunodeficiencies,
Freiburg, Germany (2013)
5. The role of flow cytometry in PID diagnostics & T cells can blur the vision on SCID, 15th ESID
Meeting, Florence, Italy (2012)
6. Tracking B cell development and reconstitution, PIDTC 2nd Annual Scientific Meeting, Boston,
USA (2012)
7. DNA repair in the immune system: the immune repertoire tells the story, Seminar Harvard Medical
School Boston, Boston, USA (2012)
8. Novel insights in molecular biological causes of (severe) combined immune deficiencies,
Paediatric Immunology and Infectious Diseases Research Symposium, Newcastle, UK (2012)
9. New developments in Primary Antibody Deficiencies, Primary immunodeficiency Meeting,
Istanbul, Turkey (2012)
10. Molecular diagnostics for PID & B & T-cell development: generation of immune repertoires. ESID
Summer school, Barga, Italy (2011)
11. Evaluation of suspected immunodeficiency. 27th European Association of Allergy and Clinical
(EAACI) Immunology meeting Istanbul, Istanbul, Turkey (2011)
12. Precursor B-cell differentiation in PID. XII Congres Sociedad Iberica de citometria Bilbao, Spain
(2011)
13. Primary Antibody Deficiencies in the context of normal B-cell differentiation. 5th European
Workshop on Immune-Mediated Inflammatory Diseases, Sitges, Spain (2010)
14. B-cell maturation defects in bone marrow & Detection of T-cell deficiencies & Flowcytometric
classification of CVID. 6th European Course on Clinical Analysis PID, Valencia, Spain (2010)
15. Hypogammaglobulinemia: so what? European Congress of Immunology (ECI), Berlin, Germany
(2009)
16. The first DNA-PKcs deficiency and a remarkable NHEJ deficiency. IUIS PID expert meeting,
Dublin, Ireland (2009)
17. Molecular diagnostics of PID. XIIIth meeting of the ESID, ’s Hertogenbosch, the Netherlands
(2008)
18. Molecular diagnostics in PID. Oxford course on Pediatric Infections and Immunity, Oxford, UK
(2007)
19. Role of flowcytometric immunophenotyping in diagnostics of primary antibody deficiencies. 6th
Euroconference on Clinical Cell analysis, Stresa (Lago maggiore), Italy (2006)
20. B-cell development and B-cell defects. The 4th School of Clinical Immunology, Progress in
Education in Clinical Immunology, Ruciane, Poland (2006)
21. Flowcytometric and molecular diagnosis of Primary Immunodeficiency Diseases. The 4th School of
Clinical Immunology, Progress in Education in Clinical Immunology, Ruciane, Poland (2006)
22. V(D)J recombination defects in T-B- SCID patients & Diagnostic strategies for PID patients
Progress in Education in Clinical Immunology, Zakopane, Poland (2004)
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