DNA und RNA Ligasen Enzyme Ligation Cofactor Temp Ligation Template Type of Ends Ligated Primary Application T3 DNA Ligase 20°C ATP Required to Ligate No T4 DNA Ligase 4°C25°C 25°C 25°C ATP No Yes Yes ATP ATP No No Yes Yes Yes Yes Ampligase™ DNA Ligase 20°C95°C NAD Yes; DNA only No Yes Taq DNA Ligase 45°65°C NAD Yes; DNA only No Yes Ligase Chain reaction CircLigase™ ssDNA Ligase 20°C65°C 20°C65°C 5°C25°C ATP No N/A Make ssDNA circles Not needed NAD No N/A Make ssDNA circles Yes; DNA only Ligates ssDNA Ligates ssDNA Weak activity Yes Make long cDNA T4 DNA Ligase (Rapid) T7 DNA Ligase CircLigase™ II ssDNA Ligase E. coli DNA Ligase Blunt Cohesive Yes Yes Tolerates High Ionic Strength Cloning Rapid Ligation 1000 fold higher activity on sticky ends than blunt ends Templatedependent ligation Heat Inactivation 15 min, 65°C No; half-life 1 hr, 95°C No; half-life 30 min, 95°C 5 min, 100°C 2 min, 100°C 20 min, 65°C Fast-Link™ DNA Ligase 20°C ATP No Yes Yes Rapid ligation T4 RNA Ligase 1 37°C ATP No Ligate RNA to DNA Ligate ssRNA to ssRNA or ssDNA T4 RNA Ligase 2 37°C ATP No Ligates ssDNA and ssRNA Nicks in ds RNA Ligating dsRNA Nicks Thermostable RNA Ligase 25°C60°C ATP No Ligates ssRNA Ligating DNA &RNA (Duplex), ssRNA N/A T4 RNA Ligase 2, Deletion Mutant 25°C Not needed No Ligates RNA Ligate DNA or RNA to RNA cDNA library 20 min, creation/ Ligation of 65°C preadenylated Adaptors to RNA Ligate ssRNA to ssRNA or ssDNA Biozym Scientific GmbH Steinbrinksweg 27 ● 31840 Hessisch Oldendorf ● Tel.: 0 51 52 / 90 20 ● Fax: 0 51 52 / 20 70 ● E-Mail: [email protected] Biozym Biotech Trading GmbH Wehlistraße 27b ● A-1200 Wien ● Tel.: 01 / 334 0156 0 ● Fax: 01 / 334 0156 88 ● E-Mail: [email protected] 10 min, 65°C 5 min, 100°C Artikel Nummern: DNA Ligasen Art. Nr. Bezeichnung 132200 T4 DNA Ligase kloniert + Puffer, 2 U/µl 133200 T4 DNA Ligase kloniert + Puffer, 10 U/µl 280220 T4 DNA Ligase, 120000 U/ml 280221 T4 DNA Ligase, 120000 U/ml 280225 T4 DNA Ligase (Rapid), 600000 U/ml 280226 T4 DNA Ligase (Rapid), 600000 U/ml 133600 Fast-Link DNA Ligations Kit 133605 Fast-Link DNA Ligations Kit 110250 Ampligase DNA Ligase + Puffer, 100 U/µl 111025 Ampligase DNA Ligase + Puffer, 5 U/µl 111075 Ampligase DNA Ligase + Puffer, 5 U/µl 111250 Ampligase DNA Ligase + Puffer, 5 U/µl 113100 Ampligase DNA Ligase, 100 U/µl 131401 CircLigase ssDNA Ligase, 100 U/µl 131405 CircLigase ssDNA Ligase, 100 U/µl 131402 CircLigase II ssDNA Ligase 131406 CircLigase II ssDNA Ligase 131420 E. coli DNA Ligase, 10 U/µl 280200 E. coli DNA Ligase, 10000 U/ml 280210 T3 DNA Ligase, 3000000 U/ml 280211 T3 DNA Ligase, 3000000 U/ml 280250 T7 DNA Ligase, 3000000 U/ml 280251 T7 DNA Ligase, 3000000 U/ml 280260 Taq DNA Ligase, 40000 U/ml RNA Ligasen 138030 T4 RNA Ligase 2, Deletion Mutant 138031 T4 RNA Ligase 2, Deletion Mutant 280245 T4 RNA Ligase 2 Truncated, 5000 U/ml 131410 Thermostable RNA Ligase 100 U/µl 280230 T4 RNA Ligase 1, 20000 U/ml 280231 T4 RNA Ligase 1, 20000 U/ml 280240 T4 RNA Ligase 2, 30000 U/ml Verpackung 2000 Units 2000 Units 150000 Units 23000 Units 240000 Units 26000 Units 100 Reaktionen 50 Reaktionen 2500 Units 250 Units 750 Units 2500 Units 10000 Units 1000 Units 5000 Units 1000 Units 5000 Units 200 Units 2500 Units 900000 Units 15000 Units 900000 Units 900000 Units 20000 Units 2000 Units 10000 Units 500 Units 1000 Units 10000 Units 1540 Units 4500 Units Biozym Scientific GmbH Steinbrinksweg 27 ● 31840 Hessisch Oldendorf ● Tel.: 0 51 52 / 90 20 ● Fax: 0 51 52 / 20 70 ● E-Mail: [email protected] Biozym Biotech Trading GmbH Wehlistraße 27b ● A-1200 Wien ● Tel.: 01 / 334 0156 0 ● Fax: 01 / 334 0156 88 ● E-Mail: [email protected]
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