RNA Polymerases Enzyme Promotor Activity Applications T7 RNA Polymerase T7 Promoter E. coli RNA Polymerase Core Enzyme E. coli RNA Polymerase Holoenzyme no Core Enzyme will generate a variety of nonspecific transcripts Studying mechanisms of transcription initiation specific bacterial and phage promoters RNA synthesis from E. coli promoter Studying mechanisms of transcription initiation Poly(A) Polymerase uses ATP as a substrate for template-independent addition of adenosine monophosphate to the 3´-hydroxyl termini of RNA molecules Poly(A) tailing of RNA for cloning or affinity purification, Enhances translation of RNA transferred into eukaryotic cells T7 RNA Polymerase produces defined Synthesis of RNA for nucleic acid RNA by in vitro transcription of dsDNA amplification methods or gene expression that is downstream of the specific RNA studies polymerase promoter SP6 RNA SP6 Promoter SP6 RNA Polymerase produces defined Synthesis of RNA for nucleic acid Polymerase RNA by in vitro transcription of dsDNA amplification methods or gene expression that is downstream of the specific RNA studies polymerase promoter T7 R&DNA™ T7 Promoter IVT: Enzyme is able to incorporate certain Synthesis of "RNA" transcripts of mixed Polymerase 2´-modified-dNTPs (including but not rNMP/2´-dNMP or rNMP/2´-modifiedlimited to dNTPs having a 2´-fluorine or NMP composition, Synthesis of modified 2´-amino group) "RNA" transcripts that are resistant to RNase A-type RNases Poly(A) Polymerase Tailing Kit Artikel Nummern Art. Nr. Bezeichnung Verpackung 140010 E. coli RNA Polymerase Core Enzyme 100 Units 140050 E. coli RNA Polymerase Core Enzyme 500 Units 141005 E. coli RNA Polymerase Holoenzyme 50 Units 141025 E. coli RNA Polymerase Holoenzyme 250 Units 145102 SP6 RNA Polymerase, 200 U/µl 10000 Units 147101 T7 RNA Polymerase, 200 U/µl 10000 Units 147201 T7 RNA Polymerase, 1000 U/µl 50000 Units 147202 T7 RNA Polymerase, 1000 U/µl 50000 Units 150490 Poly(A) Polymerase Tailing Kit, 4 U/µl 400 Units 150491 A-Plus Poly(A) Polymerase Tailing Kit, 4 U/µl 50 Reactions (400 Units) 150520 T7 R&DNA Polymerase, 50 U/µl 1000 Units 150521 T7 R&DNA Polymerase, 50 U/µl 5000 Units 280362 T7 RNA Polymerase, 50000 U/ml 50000 Units 280363 T7 RNA Polymerase, 50000 U/ml 1250 Units Biozym Scientific GmbH Steinbrinksweg 27 ● 31840 Hessisch Oldendorf ● Tel.: 0 51 52 / 90 20 ● Fax: 0 51 52 / 20 70 ● E-Mail: [email protected] Biozym Biotech Trading GmbH Wehlistraße 27b ● A-1200 Wien ● Tel.: 01 / 334 0156 0 ● Fax: 01 / 334 0156 88 ● E-Mail: [email protected]
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