RNA Polymerasen - Biozym Diagnostik GmbH

RNA Polymerases
Enzyme
Promotor
Activity
Applications
T7 RNA
Polymerase
T7 Promoter
E. coli RNA
Polymerase
Core
Enzyme
E. coli RNA
Polymerase
Holoenzyme
no
Core Enzyme will generate a variety of
nonspecific transcripts
Studying mechanisms of transcription
initiation
specific
bacterial and
phage
promoters
RNA synthesis from E. coli promoter
Studying mechanisms of transcription
initiation
Poly(A) Polymerase uses ATP as a
substrate for template-independent
addition of adenosine monophosphate to
the 3´-hydroxyl termini of RNA molecules
Poly(A) tailing of RNA for cloning or
affinity purification, Enhances translation
of RNA transferred into eukaryotic cells
T7 RNA Polymerase produces defined
Synthesis of RNA for nucleic acid
RNA by in vitro transcription of dsDNA
amplification methods or gene expression
that is downstream of the specific RNA
studies
polymerase promoter
SP6 RNA
SP6 Promoter
SP6 RNA Polymerase produces defined
Synthesis of RNA for nucleic acid
Polymerase
RNA by in vitro transcription of dsDNA
amplification methods or gene expression
that is downstream of the specific RNA
studies
polymerase promoter
T7 R&DNA™ T7 Promoter IVT: Enzyme is able to incorporate certain Synthesis of "RNA" transcripts of mixed
Polymerase
2´-modified-dNTPs (including but not
rNMP/2´-dNMP or rNMP/2´-modifiedlimited to dNTPs having a 2´-fluorine or
NMP composition, Synthesis of modified
2´-amino group)
"RNA" transcripts that are resistant to
RNase A-type RNases
Poly(A)
Polymerase
Tailing Kit
Artikel Nummern
Art. Nr.
Bezeichnung
Verpackung
140010
E. coli RNA Polymerase Core Enzyme
100 Units
140050
E. coli RNA Polymerase Core Enzyme
500 Units
141005
E. coli RNA Polymerase Holoenzyme
50 Units
141025
E. coli RNA Polymerase Holoenzyme
250 Units
145102
SP6 RNA Polymerase, 200 U/µl
10000 Units
147101
T7 RNA Polymerase, 200 U/µl
10000 Units
147201
T7 RNA Polymerase, 1000 U/µl
50000 Units
147202
T7 RNA Polymerase, 1000 U/µl
50000 Units
150490
Poly(A) Polymerase Tailing Kit, 4 U/µl
400 Units
150491
A-Plus Poly(A) Polymerase Tailing Kit, 4 U/µl
50 Reactions (400 Units)
150520
T7 R&DNA Polymerase, 50 U/µl
1000 Units
150521
T7 R&DNA Polymerase, 50 U/µl
5000 Units
280362
T7 RNA Polymerase, 50000 U/ml
50000 Units
280363
T7 RNA Polymerase, 50000 U/ml
1250 Units
Biozym Scientific GmbH
Steinbrinksweg 27 ● 31840 Hessisch Oldendorf ● Tel.: 0 51 52 / 90 20 ● Fax: 0 51 52 / 20 70 ● E-Mail: [email protected]
Biozym Biotech Trading GmbH
Wehlistraße 27b ● A-1200 Wien ● Tel.: 01 / 334 0156 0 ● Fax: 01 / 334 0156 88 ● E-Mail: [email protected]