Challenge test with Lyoflora FP-18 used on a meat emulsion sausage Product description Cooked emulsion sausages with casing. Sprayed with Lyoflora FP-18 after cooking and cooling. MAP. Test results on Chorizo sausage type. Produced 29.08.2014. Topic Challenge test with applied Listeria monocytogenes and analysed in triplicate. Total cell count/aerobic microorganisms (NMKL 86-4, 2006) and L. monocytogenes (Rapid’L.mono, BioRad qualitatively and quantatively) were analysed. Un-inoculated test Day 0: <104 CFU aerobic microorganisms/g and no L. monocytogenes in 25 g. Sensory comments None. Product Chorizo sausage Analytical data Day 0, 1 0, 2 0, 3 10, 1 10, 2 10, 3 25, 1 25, 2 25, 3 Aerob. CFU/g 1.0E+05 1.0E+04 1.0E+05 2.0E+06 6.3E+04 5.0E+04 1.0E+07 Aerob./average CFU/g 1.3E+07 6.3E+06 9.8E+06 7.0E+04 7.0E+05 List. CFU/g 9.0E+01 1.0E+02 9.0E+01 <10 1.0E+01 2.0E+01 <10 List./average CFU/g 2.0E+01 <10 6.7E+00 9.3E+01 1.0E+01 Illustration 100 1.0E+07 80 CFU/g 60 1.0E+05 40 1.0E+04 20 1.0E+03 0 0 5 10 15 Time/Days Conducted by Five Kitchen, Sweden in August 2014 20 25 30 CFU Listeria/g 1.0E+06 Aerob. List.
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