QUICK REFERENCE GUIDE

QUICK REFERENCE GUIDE
APPROXIMATE VOLUME (mL)
DRAIN
INLET
TISSUE
(AU X )
DR AIN
IN LE T
T IS S U
E
1
Puregraft System2 contents:
x8
DRAIN
INLET
x2
TISSUE
A Puregraft®
Bag
B Waste Bag
C Inlet Tubing Set
D Tissue Access Port
Adapter (TAPA)
E TAPA to Luer
Adapter
Additional supplies needed:
LR
F Puregraft 550/IS
Instrument Set
G Toomey Syringes
H Luer-lock Syringes
J Syringe Stand
I Lactated Ringer’s (LR)
(optional)
or Saline solution- 1L
1
1
K Toomey Syringe
Caps (optional)
AUX port is not used during the Puregraft procedure
Actual contents may vary based on the Puregraft size
2
STEP 1. SETUP
1C
1B
1A
3
3
For Puregraft 850, waste bag must be
connected manually.
STEP 2. TISSUE ADDITION
2A
2B
Tissue
Excess Fluid
(Only for suction-assisted harvest)
2C
Repeat Steps
2A
2 - 22D for
the entire
tissue volume 5
2D
Tissue Volumes 5
(Min-Max)
(50 mL - 250 mL)
(200 mL - 850 mL)
2
4
Tissue volume does not include excess fluids.
5
Add 30-50% more tissue than the desired graft volume.
STEP 3 . TISSUE WASHING
3A
Tissue
Lactated
Volume (mL) Ringer’s (mL)
Tissue
Lactated
Volume (mL) Ringer’s (mL)
50
50
200
100
100
100
400
200
150 - 250
150
600
300
850
425
3B
L.R.
L.R.
OR
Syringe Method
Inlet Tubing Method
3C
3D
Drain times:
15 Sec
1st
Wash
30 Sec
2nd
Wash
~3 Min
1-5 Min
2-6 Min
2-6 Min
5
5
3E
Repeat Steps 3B1 - 13E for 2nd Wash
3
5
Depending on desired graft hydration level
STEP 4. GRAFT EXTRACTION
4A
OR
Toomey Syringe Method- (Recommended)
Luer-lock Syringe Method6
GRAFT TRANSFER
Toomey Syringe
TAPA
TAPA to Luer
Adapter
Luer Lock
Syringe
6
May increase risk of clogging
Helpful tips for Puregraft Users:
1. Puregraft System works best with tissue harvested with 3mm or smaller cannulae.
2. For best results, use HSW Soft-Ject branded luer-lock syringes.
3. Do not fill the Puregraft bag beyond the recommended max tissue volumes.
4. Extract and discard any excess fluid trapped under the “Tissue” port before removing
processed graft.
5. For fibrous tissue, a small amount of LR and/or air can be introduced in to the bag
to help with tissue extraction and preventing clogging.
Puregraft, LLC.
3020 Callan Road
San Diego, CA 92121, USA
Tel: 1.858.348.8050
Email: [email protected]
www.puregraft.com
14-PG-0031-EN-GBL-A
Puregraft and the Puregraft logo are registered trademarks of Puregraft, LLC. in the United States
and other selected countries.
© 2014 Puregraft LLC. All rights reserved.
4

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