– 1384 3 2 . 1* : . . IVF IVF , 54 CO2 9 Ham'sF10 . . 50 ive 100 (DMSO) 3 45 : . %95 37°C DMSO . . . : 50 . ROC . ch IVF IVF (Exprimental) of 18 . D 4 SI 76 -81 : 50 10/5 Ar . IVF : %15 . : . (1 ) 40 . -2 -3 (IVF) 20 -4 – (ICSI) . - 1 0913 353 5129 : Email: [email protected] – (IUI) * : - - -3 -2 4 – 84/4/25: 84/3/25: www.SID.ir 2 cut-off ROC 129 10 1996 10 (5 ) 30 . Carver . (2 ) 25 15 IVF . . IVF IVF (Seminal Analysis) D . . (2 ) . SI . . (3 ) IVF . . . 1383 56 . . Ar 2 . (2 ) 1382 Exprimental ch . ive of in vitro in vivo 7 SA . . IVF IVF (Liquefaction) . 37º C 45 30 Ham’sF10 10 . 400g 600 l CO2 37º C (4 ) IVF in vivo (Capacitation) . . %95 . 200 l 1994 Calvo . 200 l 1384 IVF 232 – www.SID.ir 2 . . . SPSS . (Correlation) .(Pearson Correlation) 50 . 1 l/ml DMSO . 50 HCG HMG . 200 . l ROC D . 50 (Acrosome Reaction=AR) (AR-control) 50 .(1 ( p<0.05) ) 5 50 DMSO ) ch .(1 50 ( - 37 3 200 ) 0/8 0.1M .(2 ) (p<0.05) 10 -1 10 37º C -2 -3 20 ) . . ) 22º C ( l 500g : 10 CO2 30 . 0/8 PH=1.8 DMSO 50 . . ( Ar 50 . . ive (AR-FF50) of (AR-DMSO) . SI 18 PH=5.3 -4 -5 10 (Receiving Operating Characteristics) ROC 1 cut-off 10 /5 45 . 2 %45 . cut-off . 1384 . (Double blind) 100 ART – www.SID.ir www.SID.ir ive ch Ar of SI D 2 :1 P Value (Pearson correlation) - 0/589 (P<0.05)+ 0/041 Range SD ± Mean 0-54 25/66 ± 13/49 AR-DMSO(%) 0-48 11/82 ± 12/18 AR-FF50(%) 1 2 2: FR>50% n=40 Range SD ± Mean P<0.05 6/14 ± 7/86 0-22 13/86 ± 12/88 0-48 - 23/96 ± 17/7 0-54 6/27 ± 11/84 9-51 DMSO = = DMSO . * DMSO SI + D FR=<50% n=14 Range SD ± Mean - 1 2 ** - * ** 80 . of 70 60 (6 ) 50 in vivo . (round-headed) ZP ch . (7 ) 40 ive IVF 6 9 .7 4 30 20 5 4 .4 9 4 4 .9 1 10 0 AR - c o n tr o l AR - D M S O AR -F F 5 0 . Ar :1 DMSO . ZP A23187 . (8 ) . . (SA) . (9 ) 1384 (sperm function test) – www.SID.ir 2 10 cut-off %50 cut-off . ( r=0/68) 30 20 (Capacitation) .(10) Krausz 10 .( r=0/31) 50 Pampiglione 1993 .( r=0/34) cut-off Cummins 10 cut-off . 50 : . 50 -1 50 .(P<0.05) -2 ROC ( 45 cut-off . (... 2000 Ar ch ive ) . A23187 . (4 ) ) Baldi (11) Baker Liu Parinaud cut-off 1991 2002 of 20 . 1995 2002 D %31 117 (1991) Cummins (1994) 54 IVF IVF .(.p<0.05) Calvo SI IVF cut-off 10/5 10/5 Oehinger . %45 . . : . 1996 Carver IVF . References 1384 – www.SID.ir 2 1. Quinn P, Jouannet P, Frydman R, Van Steirteghem, A. C, Wolf, J. P, Czyglik, F, Van der Abbeel, E. Infertility , a comprehensive text; ionophore- Induced acrosome reaction. 2005 Feb: 20 (2): 469-75. 9. Cummins JM, Pember SM, Jequier AM,Yovich 2nd ed; Seibel, Machelle, M., Eds, 1997. Yovich JL, Hartmann PE. A test of the human Appleton & Lange: Stamford, 793-807. sperm acrosome reaction following ionophore 2. 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