Table S1. Saccharomyces cerevisiae strains used in this study. Strain Relevant genotype Source or Reference W303 MATa/α ade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 rad5-535 YLL934 W303 MATa mre11∆::KANMX This study YLL936 W303 MATa mre11∆::HIS3 This study YLL1169 W303 MATa rad51∆::HIS3 This study YLL1221 W303 MATa rif1∆::KANMX This study YLL1223 W303 MATa rif1∆::HIS3 This study YLL1134 W303 MATa rif2∆::KANMX This study YHN135 W303 MATa rad52∆::TRP1 This study YLL2511 W303 MATa dna2∆::NATMX pif1-M2::URA3 This study DMP5426 W303 MATα mre11∆::HIS3 This study DMP5671 W303 MATα sae2∆::KANMX This study DMP5694 W303 MATα mre11-H125N This study DMP5702/4A W303 MATα rif1∆::HIS3 This study DMP5702/7C W303 MATα rif1∆::HIS3 sae2∆::KANMX This study DMP5742 W303 MATa rif1∆::KANMX rad51∆::HIS3 This study DMP5752 W303 MATa rif1∆::HIS3 rad52∆::TRP1 This study DMP5791 W303 MATa rif1∆::HIS3 exo1∆::LEU2 This study DMP5799 W303 MATa rif1∆::HIS3 mre11-H125N This study DMP5843 W303 MATa/MATα rif1∆::HIS3/rif1∆::HIS3 MRE11/mre11∆::KANMX This study DMP5870 W303 MATa rif2∆::KANMX sae2∆::KANMX This study DMP5871 W303 MATa rif2∆::KANMX mre11∆::HIS3 This study DMP5881 DMP5882 W303 MATα rif1∆::HIS3 pif1-M2::URA3 This study YLL1523 W303 MATa/MATα rif1∆::HIS3/rif1∆::HIS3 MRE11/mre11∆::KANMX MEC3/mec3∆::TRP1 W303 MATa/MATα rif1∆::HIS3/rif1∆::HIS3 MRE11/mre11∆::KANMX RAD9/rad9∆::URA3 MATa hml∆ hmr∆ ade1 lys5 leu2-3,112 trp1::hisG ura3-52 ho ade3::GAL-HO site JKM139 sae2∆::KANMX YLL1540 JKM139 exo1∆::LEU2 This study YLL1643 JKM139 bar1∆::HPHMX This study YLL1716 JKM139 rif1∆::KANMX This study YLL 2962 JKM139 bar1∆::HPHMX rad9∆::KANMX This study YLL3096 JKM139 MEC1-9MYC::TRP1 This study YLL3366 JKM139 fun30∆::TRP1 This study YLL3378 JKM139 RIF1-18MYC::TRP1 bar1∆::HPHMX This study YLL3380 JKM139 DNA2-18MYC::TRP1 This study YLL3381 JKM139 DNA2-18MYC::TRP1 rif1∆::KANMX This study YLL3394 JKM139 rif1∆::KANMX exo1∆::LEU2 This study YLL3397 JKM139 SGS1-3HA::URA3 This study DMP5883 JKM139 This study This study 1 This study YLL3426 JKM139 RAD9-3HA::TRP1 hta2∆::NATMX hta1-S129A::URA3 This study DMP 5685/1A JKM139 bar1∆::HPHMX rif1∆::KANMX This study DMP5685/10B JKM139 bar1∆::HPHMX rif1∆::KANMX rad9∆::KANMX This study DMP5740 JKM139 rif1∆::KANMX sae2∆::KANMX This study DMP5825 JKM139 SGS1-3HA::URA3 rif1∆::KANMX This study DMP5826 JKM139 fun30∆::TRP1 rif1∆::KANMX This study DMP5840 JKM139 MEC1-9MYC::TRP1 rif1∆::KANMX This study DMP5847/1A JKM139 RFA1-18MYC::TRP1 This study DMP5847/1D JKM139 RFA1-18MYC::TRP1 rif1∆::KANMX This study DMP5880 JKM139 RAD9-3HA::TRP1 hta2∆::NATMX hta1-S129A::URA3 rif1∆::KANMX This study DMP5891/3C JKM139 RAD9-6HA::HIS3 bar1∆::TRP1 This study DMP5891/4D JKM139 RAD9-6HA::HIS3 rif1∆::KANMX bar1∆::TRP1 This study DMP5892/2D JKM139 MRE11-18MYC::TRP1 rif1∆::KANMX bar1∆::HPHMX This study DMP5892/3A JKM139 MRE11-18MYC::TRP1 bar1∆::HPHMX This study DMP5894 JKM139 EXO1-18MYC::TRP1 rif1∆::KANMX bar1∆::HPHMX This study DMP5895 This study 2 YMV45-51 JKM139 EXO1-18MYC::TRP1 bar1∆::HPHMX ho hml::ADE1 mata::hisG hmr::ADE1 leu2::leu2(Asp718-SalI)-URA3-pBR332MATa ade3::GAL::HO ade1 lys5 ura3-52 trp1::hisG YMV45 rad51∆::KANMX This study YLL3134 YMV45 exo1∆::NATMX rad51∆::KANMX This study YLL3455 YMV45 rif1∆::HPHMX rad51∆::KANMX This study YLL3462 This study 2 YMV80-51 YMV45 rif1∆::HPHMX exo1∆::LEU2 rad51∆::KANMX ho hml::ADE1 mata::hisG hmr::ADE1 his4::NAT-leu2(XhoI-Asp718) leu2-HOcs ade3::GAL::HO ade1 lys5 ura3-52 trp1::hisG YMV80 rad51∆::KANMX YLL1713 YMV80 rif1∆::HPHMX rad51∆::KANMX This study YMV45 YMV80 This study References 1. Lee SE, Moore JK, Holmes A, Umezu K, Kolodner RD, Haber JE (1998) Saccharomyces Ku70, Mre11/Rad50 and RPA proteins regulate adaptation to G2/M arrest after DNA damage. Cell 94: 399-409. 2. Vaze MB, Pellicioli A, Lee SE, Ira G, Liberi G, Arbel-Eden A, Foiani M, Haber JE (2002) Recovery from checkpoint-mediated arrest after repair of a double-strand break requires Srs2 helicase. Mol Cell 10: 373-385.
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