Table 1SM. Methodological information of sampling and laboratory determination of plasma and salivary stress biomarkers. Fluid Time of sampling Location of sampling Total cortisol Serum 8:30h - 9:30h EIS Maglumi 2000 Analyzer chemiluminescence immunoassay system (SNIBE Co, Ltd, Guandong, China). Prolactin Serum 8:30h - 9:30h EIS Maglumi 2000 Analyzer chemiluminescence immunoassay system (SNIBE Co, Ltd, Guandong, China). Assay sensitivity: 77 pmol/L. Albumin Serum 8:30h - 9:30h EIS Photometric colorimetric method using bromocresol green (Human Gesellschaftfür Biochemica und Diagnostica mbH, Wiesbaden, Germany). Fibrinogen Plasma 8:30h - 9:30h EIS Clauss method; Gernon Hemofibrin L Kit (RAL Técnica para el Laboratorio, S.A., Sant Joan Despí, Barcelona, Spain) hs-CRP Serum 8:30h - 9:30h EIS Immunoturbidimetry; Menarini Diagnostics Full Range CRP assay (Menarini Diagnostics, S.A., Badalona, Barcelona, Spain) Morning cortisol (fasting conditions) Saliva 8:30h - 9:30h EIS Chemiluminescence immunoassay (IBL, Hamburg, Germany). Cortisol awakening response Saliva Awakening, 30’ and 60’ post-awakening Home Chemiluminescence immunoassay (IBL, Hamburg, Germany). Measure Assay information (technique and manufacturer) Blood measures (fasting conditions) Salivary measures Abbreviations: EIS= Early Intervention Service; hs-CRP= High-sensitivity C-reactive protein
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