MP Biomedicals Culture Growth Media One Call One Source A World of Biotechnology Solutions Yeast 연구를 위한 total Solution •일반 배양 배지 •선택배지 (SD, DOB, YNB, CSM) •Yeast Transformation Kit •Yeast Lytic Enzyme : Zymolyase • Others www.mpbio.com MP Biomedicals Korea | 02-425-5991 Yeast Media Saccharomyces cerevisiae 일반배지 (Growth Media) YEP Broth YPD(YEPD) Agar 1리터 제조 : 30g (6 large capsules) 리터당 함량 : peptone 20g, yeast extract 10g, pH 6.5 at 30℃ 형태 제품명 1리터 제조 : 67g (67 small capsules) 리터당 함량 : YPD, agar 17g 용량 제품번호 형태 제품명 용량 제품번호 Powder YEP Broth 1 kg 114004032 Powder YPD(YEPD) Agar 1 kg 114001232 Large Capsules YEP Broth 5 kg 114004046 Pouches YPD(YEPD) Agar 10 × 1 L 114001275 Small Capsules YPD(YEPD) Agar 1 kg 114001231 YPD(YEPD) Broth 1리터 제조 : 50g (10 large capsules) 리터당 함량 : peptone 20g, yeast extract 10g, dextrose 20g, pH 6.5 at 30℃ 형태 제품명 용량 제품번호 454 g 114001022 Powder YPD(YEPD) Broth 1 kg 114001032 5 kg 114001042 Pouches YPD(YEPD) Broth 10 × 1 L 114001075 Large Capsules YPD(YEPD) Broth 1 kg 114001036 선택배지 (Synthetic Defined Media) SD Media (Synthetic Defined Media) SD Agar (Synthetic Defined Media Agar) 리터당 함량 : YNB 1.7g, Ammonium sulfate 5g, Dextrose 20g, CSM-based amino acid mixture 형태 제품명 리터당 함량 : YNB 1.7g, Ammonium sulfate 5g, Dextrose 20g, CSM-based amino acid mixture, agar 17g 용량 제품번호 10 × 0.5 L 114811065 형태 Single Dropout Formulas SD Medium - LEU Powder SD Medium - TRP SD Medium - URA 10 × 1 L 114811075 10 × 0.5 L 114812065 10 × 0.5 L 114813065 10 × 1 L 114813075 10 × 0.5 L 114823065 10 × 0.5 L 114830065 10 × 0.5 L 114842065 Double Dropout Formulas Pouches SD Medium-LEU-TRP Triple Dropout Formulas Pouches SD Medium-HIS -LEU-TRP 4+ Dropout Formulas Pouches SD Medium -ADE-HIS-LEU-TRP Pouches 제품명 용량 제품번호 SDA Medium-LEU 10 × 0.5 L 114811165 SDA Medium-URA 10 × 0.5 L 114813165 SDA Medium -HIS-LEU-TRP 10 × 0.5 L 114830165 SDA Medium -LEU-TRP-URA 10 × 1 L 114833175 Yeast Media Saccharomyces cerevisiae DOB (Drop Out Base) / DOBA (Drop Out Base Agar) 리터당 함량 : YNB 1.7g, Ammonium sulfate 5g, Dextrose 20g, (Agar 17g) 형태 제품명 용량 제품번호 227 g 114025012 454 g 114025022 1 kg 114025032 5 kg 114025042 DOB 2% Galactose (glucose-free) 454 g 114025522 DOB 2% Galactose, 1% Raffinose 227 g 114025912 454 g 114026022 1 kg 114026032 5 kg 114026042 454 g 114026522 DOB Medium Powder DOBA Medium DOBA 2% Galactose (glucose-free) YNB(Yeast Nitrogen Base) 1리터 제조 : 1.7g (YNB w/ Ammonium Sulfate : 6.7g) 리터당 함량 : 별도 table 참조 형태 제품명 YNB w/o Ammonium Sulfate Powder YNB w/ Ammonium Sulfate 용량 제품번호 227 g 114027012 454 g 114027022 227 g 114027512 454 g 114027522 1 kg 114027532 5 kg 114027542 용량 제품번호 CSM (Complete Supplement Mixture) 형태 제품명 용량 제품번호 Complete Formulation Powder 10 g 114500012 100 g 114500022 CSM-ADE 10 g 114510012 CSM-ARG 10 g 114510112 CSM 형태 CSM-LEU-TRP Single Dropout Formulation CSM-HIS Powder CSM-HIS-TRP 10 g 114510312 100 g 114510322 10 g 114510512 100 g 114510522 CSM-LYS 10 g 114510612 CSM-MET 10 g 114510712 CSM-LEU CSM-TRP 10 g 114511012 100 g 114511022 CSM-LEU-URA Powder CSM-HIS-URA CSM-HIS-LEU CSM-TRP-URA CSM-MET-URA 10 g 114511212 114511222 CSM-HIS-LEU -TRP-URA 10 g 114530112 100 g 114530122 CSM-HIS-TRP-URA 10 g 114530812 CSM-LEU-TRP-URA 10 g 114530912 10 g 114531212 100 g 114531222 Triple Dropout Formulation CSM-HIS-LEU-TRP CSM-HIS-LEU-URA 10 g 114520012 100 g 114520022 10 g 114520112 10 g 114520212 100 g 114520222 10 g 114520312 100 g 114520322 10 g 114520412 100 g 114520422 10 g 114520512 100 g 114520522 10 g 114520812 4+ Dropout Formulation 100 g CSM-URA Powder 제품명 Double Dropout Formulation Powder 10 g 114540012 100 g 114540022 CSM-ADE-HIS -LEU-TRP 10 g 114540412 CSM-ADE-HIS -LEU-TRP-URA 100 g 114550122 CSM-HIS-LEU -MET-TRP-URA 10 g 114550412 CSM-ADE-HIS -LEU-MET-TRP-URA 100 g 114560222 * Single Amino Acids 용량별 구매 가능합니다. Yeast Media Saccharomyces cerevisiae 기타 Media Components & Apparatus 형태 제품명 용량 제품번호 454 g 113072022 454 g 114019022 1 kg 114019032 3-AT 50 g 114061722 5-FOA (5-Fluoroorotic Acid) 1g 114066102 1g 114064102 5g 114064112 1g 114063102 454 g 113065522 Agar-B Agar-Y (Superior grade for yeast cloning, growth and spheroplast regeneration) Media Components IPTG X-Gal Yeast Extract-B 관련 Apparatus 1 kg 113065532 Dextrose (Glucose) 1 kg 113055032 Peptone-Y 1 kg 114018532 Replica Plating Appratus 100 mm 115000001 Velvet Pad, 6 Inch Square (Pack of 10) 100 mm 115000007 Replica Plating Appratus 150 mm 115000004 Velvet Pad, 9 Inch Square (Pack of 10) 150 mm 115000009 Roll & Grow™ Plating Beads 2 tubes 115000550 Schizosaccharomyces pombe 일반배지 (Growth Media) YES Broth (Vegetative Growth) EMM Broth 1리터 제조 : 35g 리터당 함량 : YE 5g, Dextrose 30g, 1X supplements (50mg each ADE, HIS, LEU, LYS, URA) 형태 Powder 제품명 YES Broth 형태 용량 제품번호 1 kg 114101532 Powder 제품명 EMM Broth Pouches YES Agar (Vegetative Growth) Powder 1리터 제조 : 52g 리터당 함량 : YES, Agar 17g 형태 Powder 제품명 YES Agar EMM w/o Dextrose MM w/o Nitrogen 용량 제품번호 1 kg 114101732 용량 제품번호 227 g 114110012 454 g 114110022 1 kg 114110032 10 × 1 L 114110075 227 g 114110112 1 kg 114110132 227 g 114110712 Yeast Kits & Lytic enzyme Yeast Transformation Kit 제품명 용량 제품번호 EZ-Yeast™ Transformation Kit 200 preps 112100200 Alkali-Cation™ Yeast Transformation Kit 250 preps 112200200 EZ-Yeast™ Transformation Kit Alkali-Cation™ Yeast Transformation Kit • High-throughput 실험에 적합. • 높은 efficiency • Competent cell을 만들 필요 없음. • Transformation 과정이 90분 이내 끝남 • 최소한의 hands-on time으로 간편하고 빠르게 transformation 가능 • 일반적인 효율은 plasmid DNA당 104transformants 이상임. Yeast Lytic Enzyme From Arthrobacter luteus Unit definition : The amount of activity causing a decrease in A800 of 30% Lytic Activity : 20T – 20,000 units/g 100T-100,000 units/g * This is a highly purified preparation which effectively lyses cell walls of viable yeast cells 제품명 Zymolyase 20T (activity : 20,000units/g) Zymolyase 100T (activity : 100,000units/g) 용량 제품번호 1g 08320921 250 mg 08320932 500 mg 08320931 Yeast 파쇄를 위한 Beads 제품명 Lysing Matrix C 제품번호 50 × 2ml tubes 116912050 100 × 2ml tubes 116912100 500 × 2ml tubes 116912500 관 련 제 품 (Yeast lysis 용 1mm silica beads) 용량 편리하고 강력한 Bead beater FastPrep-24™ 우수한 성능과 빠른 실험시간을 직접 경험해 보고 싶으시다면, Demo 신청 바랍니다. Lysing Matrix C Yeast Media Saccharomyces cerevisiae Synthetic defined media를 구매하는 다양한 방법 Description Potassium Phosphate monobasic mg/L 1000.000 Magnesium Sulfate 500.000 Sodium Chloride 100.000 Calcium Chloride 100.000 Biotin 0.002 Calcium Pantothenate 0.400 Folic Acid 0.002 Inositol 2.000 Niacin 0.400 p-Aminobenzoic Acid 0.200 Pyridoxine HCl 0.400 Riboflavin 0.200 Thiamine HCl 0.400 Boric Acid 0.500 Copper Sulfate 0.040 Potassium Iodide 0.100 Ferric Chloride 0.200 Manganese Sulfate 0.400 Sodium Molybdate 0.200 Zinc Sulfate 0.400 Total 1705.844 Ammonium Sulfate 5000.000 Dextrose 20000.000 Total 26705.844 Adenine 10.000 L-Arginine HCl 50.000 L-Aspartic Acid 80.000 L-Histidine HCl 20.000 L-Isoleucine 50.000 L-Leucine 50.000 L-Methionine 20.000 L-Phenylalanine 50.000 L-Threonine 100.000 L-Tryptophan 50.000 Uracil 20.000 L-Valine DOB Drop Out Base SD Synthetic Defined 100.000 L-Lysine HCl L-Tyrosine YNB Yeast Nitrogen Base 50.000 140.000 Total 790.000 Total 27495.844 www.mpbio.com CSM Complete Supplement Mixture
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