Generic Life Cycle Assessment of proteins from insects

Insects to feed the world
Wageningen, 17 May 2014
Generic
Life Cycle Assessment
of proteins from insects
Bart MUYS & Martin ROFFEIS
Earth & Environmental Sciences, KU Leuven
RESEARCH TEAM
Berta PASTOR, Paola GOBBI, Anabel MARTINEZSANCHEZ, Santos ROJO, Fen ZHU, Erik MATHIJS,
Wouter ACHTEN
CIBIO / Bioflytech SL, Universidad de Alicante, Spain
Huazhong Agricultural University, China
Department of Earth and Environmental Sciences, KU Leuven, Belgium
IGEAT Brussels, Belgium
PROTEINS FROM INSECTS
 Human population growth + dietary shifts → meat +
fish consumption ↑↑↑
 Meat + fish production → high environmental impact
(land and marine resources, GHG emissions)
 Insect protein = booming alternative for meat + fish OR
as feed for meat + fish
 Is insect protein a sustainable substitute? Impact
unexplored
 Insects = great application potential, but success will
largely depend on proven sustainability
 Consider sustainability issues from the design phase
HOW TO ASSESS SUSTAINABILITY?
 Consider all aspects of sustainability
environmental Life
Cycle Assessment
(LCA)
Life Cycle
Costing (LCC)
Social Life Cycle
Assessment
(S-LCA)
Life Cycle Sustainability Assessment (LCSA)
 Use outcomes in a learning process of continuous
improvement
WHAT IS LIFE CYCLE ASSESSMENT?


ISO 14040 standardised procedure to quantify the
environmental impact of a product
Including all input (resource use) and output (emissions)
related impacts along the complete life cycle from cradle
to grave
PROTEINSECT PROJECT: INSECT PROTEINS FOR FEED
 GOAL & SCOPE: develop sustainable production
systems of insect protein for animal feed
 INPUT: Insects reared on organic waste streams
 OUTPUT: valuable proteins suitable for fish and
monogastric livestock production.
 CONDITION: Integrate LCSA
FIRST RESULTS OF ENVIRONMENTAL LCA
Generic LCAs for small scale industrial units
Functional unit (FU): depends on the system
4 production systems:
Main
Purpose
Byproducts
Species
Substrate
Scenario
Code
Pig manure
reduction
(FU: 1kg of
waste
reduction)
Residue
Substrate +
Insect
Protein
Housefly
(Musca
domestica)
Pig manure
Fresh
manure
HF fm
Dewatered
manure
HF dm
Manual
harvest
BSF mh
Automated
harvest
BSF ah
Insect
Residue
Protein
Substrate
(FU: 1 kg of
insect
product)
Black
Soldier fly
(Hermetia
illuscens)
Brewery
waste
FIRST LCA RESULTS
1. Building a material flow chart: example for HF fm
2. Finalized material flow charts for all 4 case studies
3. Descriptive analysis based on inventory analysis for all
4 case studies
4. Impact assessment (land occupation and fossil fuel
depletion) for all 4 case studies
BUILDING THE MATERIAL FLOW CHART
Example: HOUSE FLY - FRESH MANURE – MANUAL HARVEST
1. Simple basic scheme
Insect rearing
Substrate
INPUT
Insectbased
product
OUTPUT
BUILDING THE MATERIAL FLOW CHART
Example: HOUSE FLY - FRESH MANURE – MANUAL HARVEST
2. Discerning the unit processes (≠ operating conditions)
Larvae
production
Finishing
Population
maintenance
Egg production
Substrate
INPUT
Insect
product
OUTPUT
BUILDING THE MATERIAL FLOW CHART
Example: HOUSE FLY - FRESH MANURE – MANUAL HARVEST
3. Focusing on the manure reduction (larvae + finishing)
Larvae
production
Pig fattening
Fresh
manure
Finishing
Dried
Insect
insect
product
product
Substrate
inoculation
Population maintenance
Instar larvae+
substrate
Egg production
Larvae
development
Drying
Substrate
+ pre-pupa
Harvest
Prepupa
Residue
substrate
INPUT
OUTPUT
BUILDING THE MATERIAL FLOW CHART
Example: HOUSE FLY - FRESH MANURE – MANUAL HARVEST
4. Focusing on egg production and adult population maintenance (= similar to
larvae production, but ≠ conditions + ≠ output)
Larvae
production
Pig fattening
Fresh
manure
Finishing
Dried
Insect
insect
product
product
Substrate
inoculation
Population maintenance
Substrate
inoculation
Fly eggs
Instar larvae
+
substrate
Egg production
Mating
Substrate
+ Pupa
Refined
sugar
Feeding
Harvest
Milk
powder
Adult flies
INPUT
Pupa
hatch
Larvae
development
Drying
Larvae
development
Ovipositio
n
Water
Instar larvae+
substrate
Pupa
Substrate
+ pre-pupa
Harvest
Residue
substrate
Prepupa
Residue
substrate
OUTPUT
BUILDING THE MATERIAL FLOW CHART
Example: HOUSE FLY - FRESH MANURE – MANUAL HARVEST
5. Adding remaining flows + determining optimal conditions of unit processes
Larvae
production
22 - 26° C
Substrate
Φ 45
- 60
inoculation
Pig fattening
Fresh
manure
Population
maintenance
Substrate
inoculation
22 - 26° C
Φ 45 - 60
Instar larvae
Dead flies
Unhatche
d pupa
Fly eggs
Cleaning
Eggrearing
production
cages
23 - 27° C
Φ 50 - 60
+
substrate
Mating
Substrate
+ Pupa
Refined
sugar
Feeding
Harvest
Milk
powder
Adult flies
INPUT
Pupa
hatch
Larvae
development
Dried
Insect
insect
product
product
Packaging
Drying
Larvae
development
Ovipositio
n
Water
Instar larvae+
substrate
Finishing
0 - 30° C
Φ 30 - 90
Pupa
Substrate
+ pre-pupa
Harvest
Residue
substrate
Residue
substrate
Prepupa
Packaging
OUTPUT
Insect rearing system - Unit processes and fundamental material flows
Pretreated pig manure, manual harvest, dried insect product [HFdm] (Alicante, Spain)
House fly [Musca domestica]
Pig fattening
Pretreated
manure
Dead flies
22 - 26° C
Φ 45 - 60
Fresh
manure
Substrate
inoculation
Fly eggs
Cleaning
rearing cages
Instar larvae +
substrate
Egg production
23 - 27° C
Φ 50 - 60
Larvae
development
 240 h
Oviposition
Mating
Substrate
+ Pupa [PM]
Feeding
Harvest
Pupa
hatch
INPUTS
Unit
process
Process
Material flow
Packed, dried
insect product
Product
Pupa
[PM]
Instar larvae+
substrate
watering
Larvae
development
 240 h
Substrate
+ Pupa [LP]
Packed residue
substrate
Pupa
[LP]
Residue
substrate [LP]
Residue
substrate [PM]
Packaging
Drying
150° C,  4 h
Harvest
Adult flies
Milk powder
10 - 30° C
Φ 30 - 90
Population maintenance [PM]
Unhatched
pupa
Refined
sugar
Finishing
22 - 26° C
Φ 45 - 60
Substrate
inoculation
Dewatering
Water
Larvae production [LP]
Packaging
OUTPUTS
Conversion degree
Final
product
Process
residues
Insect rearing system - Unit processes and fundamental material flows
Brewery waste, manual harvest, dried insect product [BSFmh] (Alicante, Spain)
Black Soldier Fly [Hermetia illuscens]
Brewery
Brewery
waste
Larvae production
Finishing
25 - 30° C
Φ 40 - 80
10 - 30° C
Φ 30 - 90
Packed, dried
insect product
Substrate
inoculation
Dead flies
Seed larvae
Unhatched
pupa
Hen feed
Egg production
Cleaning
rearing cages
21 - 30° C
Φ 40 - 70
27 - 29° C
Φ 50 – 70
Mixing
Water
Packaging
Larvae hatch
Instar larvea
development
 216 h
Fly eggs
Oviposition
Seed larvae +
substrate
Larvae hatch
Drying
150° C,  4 h
Larvae
development
 240 h
Breeding
stock
selection
Residue substrate
+ pre-pupa
Mating
Pupation
Refined
sugar
Larvae
collection
Harvest
27 - 29° C
Φ 50 - 80
Feeding
Pupa
hatch
INPUTS
Unit
process
Process
Material flow
Product
Packed residue
substrate
Packaging
Adult flies
Pupa
Pupa
development
 192 h
Conversion degree
Pre-pupa
OUTPUTS
Final
product
Process
residues
Insect rearing system - Unit processes and fundamental material flows
Brewery waste, semi-automated harvest, dried insect product [BSFah] (Alicante, Spain)
Black Soldier Fly [Hermetia illuscens
]
Brewery
Brewery
waste
Larvae production
Finishing
25 - 30° C
Φ 40 - 80
10 - 30° C
Φ 30 - 90
Packed, dried
insect product
Substrate
inoculation
Dead flies
Seed larvae
Unhatched
pupa
Hen feed
Egg production
Cleaning
rearing cages
21 - 30° C
Φ 40 - 70
Larvae hatch
27 - 29° C
Φ 50 - 70
Instar larvea
development
 216 h
Mixing
Fly eggs
Oviposition
Water
Larvae hatch
27 - 29° C
Φ 50 - 80
Feeding
Residue substrate
+ remaining prepupa
Pupa
hatch
Process
Material flow
Product
Breeding
stock
selection
Larvae
collection
Adult flies
Unit
process
Packed residue
substrate
Packaging
Pre-pupa
migration
 72 h
Pupation
INPUTS
Drying
150° C,  4 h
Larvae
development
 240 h
Mating
Refined
sugar
Packaging
Seed larvae +
substrate
Pupa
Pupa
development
 192 h
Conversion degree
Cleaning
bio-digester
Pre-pupa
OUTPUTS
Final
product
Process
residues
HOUSE FLY REARING FOR PIG MANURE REDUCTION
Material flow: outputs per 1 kg pig manure reduction
Output residue substrate [kg DM] per input manure [kg DM]
1
Output insect product [kg DM] per input manure [kg DM]
0,9
0,8
0,7
kg
0,6
0,5
0,4
0,3
0,2
0,1
0
HFfm
HFdm
 High conversion rate into valuable products
 Manure dewatering useful if residue substrate is valuable
HOUSE FLY REARING FOR PIG MANURE REDUCTION
Life Cycle impacts (ReCIPe midpoint categories) per kg of manure reduction [DM]
Water depletion
Agricultural land occupation
m2a
3,5
3
2,5
2
1,5
1
0,5
0
Production
Maintenance
HFfm
HFdm
80
70
60
50
40
30
20
10
0
m3
Production
HFfm
Maintenance
HFdm
Fossil depletion
4
3,5
3
2,5
2
1,5
1
0,5
0
Kg oil eq
Production
Maintenance
HFfm
HFdm
 Dewatering has substantial impact by lower system efficiency
 Large contribution of unit processes dedicated to maintenance
HOUSE FLY REARING FOR PIG MANURE REDUCTION
Life Cycle impacts (ReCIPe midpoint categories) per kg of manure reduction [DM]
HFfm [0 € / m3]
HFfm [6,50 € / m3 ]
HFfm [16,20 € / m3 ]
5
4
3
2
1
0
Agricultural land
occupation
50
49
48
47
46
45
44
43
42
41
40
HFfm [0 € / m3]
HFfm [6,50 € / m3 ]
HFfm [16,20 € / m3 ]
Water depletion
5
4
3
2
1
0
HFfm [0 € / m3]
HFfm [6,50 € / m3 ]
HFfm [16,20 € / m3 ]
Fossil depletion,
calculated in kg oil eq
per kg manure
reduction [DM]
 Value of manure reduction lowers impact dramatically (economic
allocation to pig rearing)
BLACK SOLDIER FLY REARING FOR PROTEIN PRODUCT
Material flow: yield per 100 kg input substrate
Insect product yield [kg DM] per substrate
input [100 kg DM]
80
Residue substrate yield [kg DM] per
substrate input [100 kg DM]
70
60
50
40
30
20
10
0
BSFmh
BSFah
Good conversion rate, a little affected by automation
BLACK SOLDIER FLY REARING FOR PROTEIN PRODUCT
Energy and labour input per kg insect product
Energy input
KWh
12
Production
Maintenance
Labour input
h
0,4
Production
Maintenance
0,35
10
0,3
8
0,25
6
0,2
0,15
4
0,1
2
0,05
0
0
BSFmh
BSFah
BSFmh
BSFah
 Large efficiency improvement by automation
 Minor impact due to maintenance
BLACK SOLDIER FLY REARING FOR PROTEIN PRODUCT
Life Cycle impacts (ReCIPe midpoint categories) per 1kg of insect product [DM]
m2a
Agricultural land
occupation
0,09
Production
0,08
Water depletion
m3
12
Production
Maintenance 10
0,07
0,06
Maintenance
8
kg oil
eq
3,5
Fossil depletion
Production
3
Maintenance
2,5
2
0,05
6
0,04
1,5
0,03
4
0,02
0,01
0
BSFmh
BSFah
1
2
0,5
0
0
BSFmh
BSFah
BSFmh
 Large efficiency increase by automation
 Minor environmental cost of population maintenance
BSFah
BLACK SOLDIER FLY REARING FOR PROTEIN PRODUCT
Life Cycle impacts (ReCIPe midpoint categories) per 1kg of insect product [DM]
m2a
5
BSFah [0 € / ton BW]
BSFah [20 € / ton BW ]
BSFah [50 € / ton BW]
4
3
2
1
0
Agricultural land
occupation
m3
10
9
8
7
6
5
4
3
2
1
0
5
kg oil eq
4
3
2
1
0
Water depletion
Fossil depletion
 Higher value of brewery waste leads to higher impacts
BLACK SOLDIER FLY REARING FOR PROTEIN PRODUCT
Life Cycle impacts (ReCIPe midpoint categories) per 1kg of insect product [DM]
m2a
Agricultural land occupation
Kg oil eq
3
1,4
2,5
1,2
Fossil depletion
1
2
0,8
1,5
0,6
1
0,4
0,5
0,2
0
0
soybean Fishmeal
meal
BSFah
[0€/ton
BW]
BSFah
[10€/ton
BW]
soybean Fishmeal
meal
BSFah
[0€/ton
BW]
BSFah
[10€/ton
BW]
 Benchmarking against related products reveals
strengths and improvement challenges
TAKE HOME MESSAGES
1) Insect production is a versatile system to make
agriculture more efficient (adding value, waste
reduction)
2) Systems are difficult to compare (different goals,
different species, different technological equipment)
3) Alternative production scenarios result in different
system performances and efficiencies = room for
improvement
4) A large part of the impacts is due to non-productive
processes (maintenance of the population)
5) Value of substrates and products affects environmental
performance (economic allocation)
TAKE HOME MESSAGES
6) Benchmarking of this novel technology with highly
optimized related products (e.g. soya) shows potential
and challenges for improvement (e.g. in energy
requirements)
7) LCA helps to identify the crucial steps for improvement
8) Life cycle approach will also contribute to improving
social cost and economic profit
9) Golden rule of innovation for transition: do not CLAIM
before you KNOW (cf. the self-destruction of the
Jatropha tropical biofuel hype)
10)Sustainability is an issue of the START, not of the END
THANK YOU FOR YOUR ATTENTION
[email protected]