RNA Purification Transcriptome enrichment without ribosomal RNA for improved microarray analysis RiboMinus™ Transcriptome Isolation Kits →→ Comprehensive, ribosomal RNA–free, whole transcriptome analysis →→ Increased representation of RNA transcript species →→ Variety of sample formats (human/mouse, yeast, and bacterial) A enriches RNA transcripts by depleting 18S 18S 28S 18S Total RNA Total RNA 28S RiboMinus™ RNA RiboMinus™ RNA Fluorescence Kits use a novel purification system that Total RNA Total RNA 28S RiboMinus™ RNA RiboMinus™ RNA 28S 18S Fluorescence Isolation Fluorescence Transcriptome Fluorescence RiboMinus™ B large ribosomal RNA molecules from a total RNA sample. These kits use a Migration timeMigration time Migration timeMigration time 5’-biotin–labeled Locked Nucleic Acid C (LNA™) probe specific for large rRNA D to remove 95–98% of rRNA molecules Total RNA Total RNA 25/26S 25/26S 16S Fluorescence 16S Total RNA Total RNA RiboMinus™ RNA RiboMinus™ RNA Fluorescence total RNA (Figure 1). Depletion of rRNA 23S RiboMinus™ RNA RiboMinus™ RNA Fluorescence Fluorescence from human, mouse, bacterial, or yeast 23S 18S 18S enriches the representation of non– poly(A)-tailed RNA, preprocessed RNA, small tRNAs, siRNA, snRNA, microRNA, Migration timeMigration time Migration timeMigration time fragmented mRNA, and other regulatory RNAs that cannot be purified via polyA+ selection methods. Figure 1—Greater than 95% rRNA depletion from human, mouse, yeast, and bacterial samples. Agilent 2100 Bioanalyzer data demonstrating rRNA depletion. A. Human total RNA and RiboMinus™ RNA isolated from HeLa cells. B. Mouse total RNA and RiboMinus™ RNA isolated from mouse liver. C. Bacteria total RNA and RiboMinus™ RNA isolated from E. coli. D. Yeast total RNA and RiboMinus™ RNA isolated from S. cerevisiae. RNA Purification The purified RNA is concentrated and suitable for numerous downstream applications, including microarray analysis (Figure 2), library construction, and qRT-PCR. Random priming RiboMinus™ RNA D Total RNA Oligo(dT) priming C Oligo(dT) priming B Random priming A RiboMinus™ RNA Total RNA Figure 2—Improved microarray intensities from RNA enriched using the RiboMinus™ Transcriptome Isolation Kit. The arrays were assayed with 0.5 µg of either RiboMinus™ enriched RNA or total RNA. Arrays using RNA enriched with the RiboMinus™ Transcriptome Isolation Kit yielded better overall spot intensity and a higher signal-tobackground ratio than those using total RNA. A. Random priming of mRNA isolated using the RiboMinus™ Transcriptome Isolation Kit B. Random priming of total RNA C. Oligo(dT) priming of mRNA isolated using the RiboMinus™ Transcriptome Isolation Kit D. Oligo(dT) priming of total RNA. Ordering information For a comprehensive and truly representative spectrum of RNA transcripts present in the transcriptome, order RiboMinus™ Transcriptome Isolation Kits today. Product RiboMinus™ Transcriptome Isolation Kit (Human/Mouse) Quantity Cat. no. 6 preps K1550-02 RiboMinus™ Transcriptome Isolation Kit (Yeast) 12 preps K1550-03 RiboMinus™ Transcriptome Isolation Kit (Bacteria) 12 preps K1550-04 RiboMinus™ Concentration Module 6 preps K1550-05 Magna-Sep™ Magnetic Particle Separator 1 each K1585-01 Related products For current prices, visit www.invitrogen.com/rnapreps. www.invitrogen.com ©2008 Invitrogen Corporation. All rights reserved. These products may be covered by one or more Limited Use Label Licenses (see Invitrogen catalog or www.invitrogen.com). By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. For research use only. Not intended for any animal or human therapeutic or diagnostic use, unless otherwise stated. F-075051-r1 0208
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