guia rbm 2014 sin prod descatalogados a3.FH11

DNAGuide
Isolation Guide
DNA Isolation
RBME01/02
REALPURE
Saliva
Genomic DNA
RBMEG06/07
REAL SALIVA
Sample Collection
NBlood: 5 ml to 10 ml.
Saliva:100 ml to 5 ml.
Semen:100 ml to 5 ml.
N GS
d
Teste
Saliva samples, buccal cell samples via
mouth wabs, saliva samples via REAL
SALIVA DNA sample collection kit, saliva
samples via Oragene saliva collection kit.
RBMEG04/05
REALPURE
SPIN
RBMEGS01/02
REALPURE
SPIN
Food-Stool
Plants and fungi:
20 mg to 500 mg.
NG
ted
S Tes
RBMEGS10/11
NEW
REAL FFPE
RBMEGS12
Whole blood, serum, plasma, body fluids:
300 ml.
N GS
d
Teste
Tissue samples: < 10mg.
Cultured cells: < 105
Blood samples: < 100 ml
Lasser microdissected tissue:one
N GS
RBMEGS13/14
REALPLASMID
SPIN
midi/maxiprep
RBMEPS04/05
200 ml serum, plasma and cell-free
biological fluids.
RBMEGS07
Spin Columns.
For a fast manual simultaneous isolation
of viral nucleic acids.
High quality viral DNA/RNA obtained
that can be directly used in PCR or RTPCR.
Solutions
Fast clean-up of PCR products.
Fragments extraction from agarose gel 200 pb 10 Kb.
Concentration and salts removal of DNA in solutions.
Organic solvents are not used. Spin columns.
Protocol done in 10 minutes.
REALTOTAL RNA Tissues: 5 to 100 mg.
Tissues and Cells Cells: 100 to 10x106.
"STAR"
Specially designed for RT-PCR. Includes all the
necessary reagents to remove contaminant DNA.
Toxic reagents are not used.
RBMER01
RBMER02
Fast clean-up of PCR fragments 100 bp - 10
Kb.
Organic solvents are not used.
Spin columns, the protocol is done in 10 minutes.
Biological fluids (serum, plasma,
REALTOTAL
etc), bacteria, yeast,
RNA SPIN PLUS saliva,
paraffin-embedded tissue and
Remove dideoxy terminators and not
incorporated dye-labelled nucleotides from
sequencing reactions, previous to analysis in
automatic sequencers. Prehydrated matrix ready
.
to use
25 - 150 ml
culture volume.
The customer chooses the size of the bacteria
cultures wich he wants to work. Toxic reagents
are not used.
Fast and simple method.
Rapid and non-expensive.
9
REALTOTAL RNA Bacteria: 1 ml ;710 .
Bacteria & Yeast Yeast: 1ml ; 10 .
"STAR"
Specially designed for RT-PCR. Includes all the
neceseary reagents to remove contaminant DNA.
Toxic reagents are not used.
RBMER03
Rapid purification and concentration of highquality DNA from PCR or enzymatic reactions
with extremely small elution volume. Specially
designed microcolumns. Protocol is done in 2
minutes.
Plasmidic DNA can be used in PCR, restriction
analysis, subcloning, transforming and
sequencing by capillary electrophoresis. Toxic
reagents are not used.
RBMER04
REALTOTAL RNA Blood: 300 ml.
SPIN BLOOD
RBMER12
REALTOTAL RNA
SPIN Plants &
Fungi
REALPURE SPIN Cultured animal cells, tissue
samples, blood, bacteria, yeast,
DNA/RNA Kit
fungi, biologicals fluids and
RBMER16
Protects and stabilizes the genomic DNA for its
following extraction. Avoids the need of having
to process the samples inmediately. An
alternative to the use of paraffin.
Animal tissue samples, cultured
cells and bacteria.
DNA
REMOVE
It is a detergent mixture to remove
DNA and RNA from working
surfaces.
It degrades contaminant DNA and RNA at PCR
sensivility levels.
RD056
It removes RNases contamination
from glass and plastic material
surfaces.
It works by contact and it is supplied with an
applicator to be vaporized.
NUCLEASE
FREE WATER
Water for Molecular Biology
applications.
Water for PCR and DNA/RNA isolation. It is
deionized, autoclavated, filtered and non-treated
with DEPC.
RD055
RNase
REMOVE
REALSAFE
RBMSAFE
Nucleic acids staining solution.
Spin Columns.
RBMER15
REALSTAR
RBMER10
Used for DNA and RNA detection. Non mutagenic,
non carcinogenic.
Plants and Fungi: <100 mg.
High quality DNA-free total RNA is obtained.
Contains a Stabilizing Solution wich allows a safe
transport from the collecting place to the laboratory.
High quality DNA-free total RNA is obtained.
Contains a PVP solution to remove carbohydrates
and polyphenols and 2 differents Lysis Solutions.
RBMER13
To isolate high purity transfection grade plasmid
DNA from bacterial cell lysates. Includes gravityflow columns and necessary reagents for
ultrapure plasmid purification. Includes specialized
filters to optional remove cellular debris from
lysates.
REALStock
RBMES01/02
reactions cleaning.
High quality, DNA-free total RNA, is obtained.
Total RNA can be used in applications such as
RT-PCR, Northern, primer extension, array
technology and Rnase protection.
REALTOTAL RNA Bacteria: 1 ml ;7109.
Bacteria & Yeast Yeast: 1ml ; 10 .
ARNzol Kit
RD057/RD058
REALPURE
SPIN
viral DNA/RNA
The method can be scaled. Rapid and
non-expensive. Toxic reagents are not used.
1.5-3.0 ml
culture volume.
MIDI (25ml high-copy plasmids /
100ml low-copy plasmids).
MAXI (100ml high-copy plasmids
/ 500ml low-copy plasmids).
When to use
REALTOTAL RNA Tissues: 5 to 100 mg.
Tissues and Cells Cells: 100 to 10x106.
Very useful in inserts screenings of recombinants
bacteria colonies. Fast, simple and inexpensive
method.
Toxic reagents are not used.
RBMEPS02
Sample
Volume Sample
Fast clean-up of PCR products.
Fragments extraction from agarose gel 200 pb -10
Kb.
Concentration and salts removal of DNA in solutions.
Organic solvents are not used, simple and
inexpensive method. Protocol is done in 30
minutes.
1.5-3.0 ml
culture volume.
RBMEPS01/02
HIGH PURITY
REALPLASMID
Midi/Maxiprep
To isolate genomic and mitochondrial DNA
from small samples. Complete removal of
PCR inhibitors. High quality DNA that can
be directly used in PCR, Southern, any
enzymatic reaction, cloning, etc.
Samples of plants and fungi. It contains a
PVP solution to remove inhibitors. DNA
isolated in just 30 min.
PCR or enzymatic
reactions: 10 ml.
RBMEP01
Rapid purification of high-quality, ready to
use DNA from blood samples. Complete
removal of contaminants and inhibitors for
reliable downstream applications.
To isolate genomic DNA from "FFPE"
samples. Low elution volume: 15-30ml.Safe
method, avoids the use of xilene or any
other toxic reagents.
Sequencing reactions:
10 - 75 ml.
RBMCS04/05
d
Teste
Formalin fixed paraffin embedded
samples.
GS T
REALPURE NPlants
and fungi samples.
SPIN
Plants & Fungi
Viral
DNA/RNA
REAL Clean
& Concentrator
Micro Spin
REALPLASMID
SPIN miniprep
d
Teste
N GS
PCR fragments.
Product
WhenWhen
to useto use
RBMER11
REALSPIN
DTR
Low amounts of partially degrade DNA can be
purified from complex matrix.
Complete removal of PCR inhibitors and
detection of specific DNA in animals, or GMO
in food products,DNA isolation from fecal
specimens.
To isolate PCR ready bacterial DNA from preenrichment or enrichment cultures from different
food samples and stool samples. PCR and
Real Time PCR ready DNA. Complete removal
of PCR inhibitors. Includes Proteinase K and
Lysozyme.
PCR fragments.
DNA from agarose gel.
DNA concentration.
RBMCS03
Total DNA from food and stool samples:
Up to 200 mg.
Bacterial DNA:
1.5 ml. of medium culture.
PCR fragments.
DNA from agarose gel.
DNA concentration.
RBMS01/01XL
Large samples of plants and fungi. It
contains a PVP solution to remove
inhibitors. Toxic reagents are not used, the
method can be scaled. Rapid and
inexpensive.
ested
NEW
REAL Clean
Spin PCR
REALPURE
miniprep
RBMEGS08/09
REALPURE
MicroSPIN
RBMCS01/02
All types of samples: cultured cells,
animal tissues, mouse tail, paraffinembedded tissues, bacteria, yeast, body
fluids (saliva, serum,etc), hair, bloodstain.
RBMEGS03/04
REALPURE
SPIN Blood
REAL Clean
Spin
Allows to process any kind of sample.
High quality DNA obtained that can be
directly used in PCR, Southern, any
enzymatic reaction, cloning, etc. .
RBMEGS05/06
REALPURE
SPIN Food-Stool
"Bacteria"
Genomic DNA extraction from saliva samples.
Toxic reagents are not used. Rapid and
inexpensive.Contaminant RNA can be
removed with a RNase treatment.
Painless and non-invasive sample collection.
Suitable for Next Generation Sequencing. Non
toxic solution that allows the storage of saliva at
room temperature, preserving and stabilizing
DNA for its following extraction.
Saliva: 2ml.
RBMESAL01/010
REALPURE
Plants & Fungi
Large volumes of whole blood, saliva and
semen. Toxic reagents are not used. The
method can be scaled.
Rapid and inexpensive.
RBMCM01
SampleSample
VolumeVolume
SampleSample
Micro RNA
REALPURE
DNA "SSS"
ested
GS T
REAL Clean
Matrix
Allows to process any sample type. Contact
our laboratory for a specific protocol for
your sample.Toxic reagents are not
used.The method can be scaled.Rapid and
inexpensive.
Plasmid DNA
RBMEG01/02/03
animal tissues, mouse tail, paraffinembedded tissues, bacteria, yeast, body
fluids (saliva, serum,etc), hair, bloodstain.
.
Reagents
REALPURE
ested
GS T
NAll
types of samples: cultured cells,
Product
Product
When to use
DNA Fragments
Sample
Volume Sample
Product
RNA Isolation Guide
Total RNA
DNA Isolation Guide
REALTOTAL
MicroRNA Kit
RBMER14
Solutions
plants.
Cultured animal cells, tissue
samples, blood, bacteria, yeast,
fungi, biologicals fluids and plants.
The kit purifies all sizes of RNA, from large mRNA
and ribosomal RNA down to microRNA (miRNA)
and small interfering RNA (siRNA).
Rapid and very economical method.
Does not eliminate genomic DNA.
Total RNA from all types of
samples.
To remove contaminant DNA from RNA samples
and for removing the DNase after the treatment.
Fast and easy method. The DNase removal step
takes place in just 3 minutes.
Cultured animal cells, tissue
samples, blood, bacteria, yeast,
fungi, biologicals fluids and plants.
Rapid and efficient method for the isolation and
purification of small RNA molecules (<200 nt).
These small RNAs include regulatory RNA
molecules such as microRNA (miRNA) and short
interfering RNA (siRNA).
Spin Columns.
3
Rapid method for the isolation and purification
genomic DNA and total RNA simultaneously from
a single sample.
The kit purifies all sizes of RNA, from large mRNA
and ribosomal RNA down to microRNA and small
interfering RNA.
Solutions
n
mn
Electrophoresis Products
RBMM1/RBMM2
Markers Lambda DNA Digested: Marker nº 3 - Marker nº 4 - Marker Nº7 - Marker Nº 8.
RBMM3/RBMM4/RBMM7/RBMM8
Marker V5 and Marker V6.
RV05/RV06
Prepared agarose gels with Ethidium Bromure or REAL Safe.
See ref. in our website
Concentrated Buffers (TAE, TBE, TRIS-GLICINE....).
See ref. in our website
PCR Plastic Tubes. 0.2 & 0.5ml. Flat and Round cap. Assorted colors.
See ref. in our website
Horizontal
Electrophoresis
Scale Nº 1 and Scale nº 2.
Vertical
Electrophoresis
2014 - 2015
Reference
Molecular
W. markers
Description
Consumables
Product
R
RealSub Mini.
Three tray options 7x7cm, 7x10cm and Duo.
RELSMINI - 7/10/DUO
RealSub Midi.
Three tray options 10x7cm, 10x10cm and Duo.
RELSMIDI - 7/10/DUO
RealSub Plus.
Wide tray range: 15x7, 15x10, 15x15cm and Trio.
RELSPLUS - 7/10/15/TRIO
RealSub Maxi.
Resolution of high number of samples.
REALSMAXI - 20/10/DUO/25
RealSub Screen.
Rapid Screening of large number of samples.
Mini Fast.
Comet.
Completely self contained system for quick check of samples.
Single Cell Gel Electrophoresis.
REALSSCREEN - 32/24/16/TRIO
RELFMMS10
REL-COM - 10/20/40/80
Cellulose-Acetate System.
For use in diagnostic tests.
REL-CELLAS
RealPage Mini.
Preferred unit for routine protein mini-electrophoresis.
REL- VS10DSYS/CVS10DSYS
RealPage Mini Wide.
20cm Width, for those with >20 samples to compare.
RELVS - 10WD/10WDSYS
RealPage Maxi.
Up to 300 samples per run.
RELVS -20/30 -D/DSYS
RealPage Modular Systems. For multiple electr. techniques (10x10, 10x20 & 20x20 cm).
FlatBed IEF System.
Full range of IEF flat bed techniques. Inc. enhanced cooling.
Electroblotting Modular Syst.. Complete Mini/Wide/Maxi gel casting running and electrob. sys. REL -VS10CBS/CVS10CBS
Side by side transfer of multiple gels in a single set up.
REL_ -CAPL/CAPS
Semi Dry Blotters.
For Western, Southern or Northern blotting.
RELSD -10/20/33/50
Dot and Slot Blotters.
Clone/Immunological screening in Southern/Northern/Western. REL -D48/D96/S24/S48
Large Format Vertical.
Enhanced gel resolution and easy to use.
RELQ -20/33
Power
Supplies
Real Mini.
Ultra compact power supplies with dual outlets.
RELMINI -150/30
Real Midi.
Ideal for routine horizontal and mini vertical electrophoresis.
Programmable, high performance, easy to operate supplies.
REL -250/300
Midi/Maxi Gel Dryer.
21 x 31cm and 35 x 45cm drying areas.
Vapour trap + High efficiency diaphragm pump.
REL_GDVH / REL_GDVH35
Accesories
Blotting
Units
Up to 5 gel blot cassettes used at any one time.
Capillary Blotting.
Gel
Drying
RELEBM -10/20
STAND Electroblotters.
Real Maxi.
GelMaster Vacuum Pump.
REL -500/3AP
Molecular Biology
Product Guide
REL _GDPUMP
Registered Trade mark of Durviz, S.L.
Gel Levelling Table.
Useful way to pouring gels of uniform thickness.
Buffer Save Blocks.
Reduce the volume in positive and negative compartments.
Combi-comb/loading guides. Combine the convenient comb with the appropriate guide.
Distributed by:
Gel Platform and CoolPack. Unique. Cool the chambers without external chilling.
Extensive range of combs.
Full range of combs with four thicknesses and colour coded.
If you have any questions about the products in the catalogue,
please contact us at [email protected]
You can also visit our website www.reallaboratory.com for further
information.
Durviz, S.L.
Parque Tecnológico de Paterna
Leonardo da Vinci,10
46980 Paterna
Valencia - Spain
Tel: +34 96 136 61 07
Fax: +34 96 136 61 68
Email: [email protected]
Valencia - Madrid - Barcelona - Granada -La Coruña
is a registered trademark of Durviz, S.L.
RBMG102014

Download Report