テクニカルノート：NanoPhotometerのサンプル圧縮テクノロジー

Technical Note
The patented Sample Compression Technology™ provides
of even thickness, is formed eliminating the need for
unmatched
precision and
accuracy with reliable measure#2
Sampl e Compression
Technology™
ment geometry even for challenging samples including
surface tension. At the same time the sealed micro-
proteins and products in volatile solvents. This technology
テクニカルノート
environment limits evaporation. From below, light shines
through the sample, is reflected by the upper mirror and
is especially designed for ultra-low sample volumes of
Introduction
0.3 µl to 2 µl.
The patented Sample Compression Technology™ provides
returned through the lower quar tz window in the pedestal
A capillary film, shaped into a precisely defined flat layer
(see figure 1 orange arrows) onto the detector.
of even thickness, is formed eliminating the need for
unmatched
precision and accuracy with reliable measure#2 サンプル圧縮テクノロジー
Principl e of Smal l Vol ume Measurements
ment geometr y even for challenging samples including
The sample concentration in UV/ VIS spectroscopy
proteins and products in volatile solvents. This technology
is calculated based on the Beer-Lamber t law:
is
designed for ultra-low sample volumes of
序especially
文
surface tension. At the same time the sealed microThe actual distance between the two quar tz surfaces is
environment limits evaporation. From below, light shines
half of the path length as a result. This reduced distance
through the sample, is reflected by the upper mirror and
has an immensely positive influence on the drop stability
表面張力に依存することなく液柱を作り、光路を確保します。
returned
through the lower quar tz window in the pedestal
especially for challenging samples like proteins or samples
(see
figure 1 orange arrows) onto the detector.
同時に閉鎖環境がサンプルの蒸発を防ぎます。光がサンプルを
with volatile solvents.
通って上部の鏡に到達・反射して下部の台座のクォーツ面を通
The
actual distance between the two quar tz surfaces is
って検出器に届きます（図１、黄色の矢印）。
The NanoPhotometer ® NP80/ N60/ N50 always reads the
half of the path length as a result. This reduced distance
0.67 mm path length first and only if the sample is not in
２つのクォーツ表面間の実際の距離は、光路長の半分です。距
has an immensely positive influence on the drop stability
the linear range, moves the pedestal to form the second
離を短くすることにより、蛋白質溶液や揮発性溶媒中のサンプ
especially
for challenging samples like proteins or samples
shor ter 0.07 mm path (figure 2).
ルにおいて、特にドロップの安定性に非常に良い影響をもたら
with
volatile solvents.
0.3 µl to 2 µl.
サンプル圧縮テクノロジー（特許取得済み）は、蛋白質溶液や揮
c = concentration (g/ l)
発性溶媒中のサンプル測定時も、比類のない精度と正確性を提供
1 1
A = absorbance value
c = A x e of
x Smal l Vol ume Measurements
Principl
Ɛ d
Ɛ = extinction coefficient
of sample (g*cm/ l)
します。このテクノロジーは、0.3
〜2.0 μL の微量測定のために
The sample concentration
UV/ VIS
spectroscopy
D = pathinlength
(cm)
設計されました。
is
calculated based on the Beer-Lamber t law:
This equation describes the correlation between concen微量測定の特徴 c = concentration (g/ l)
tration,1 absorbance
and path length.
1
A = absorbance
value Measurements need
cUV/VIS
= A x 分光高度計において、サンプルの濃度は
x
BeerƐ d the linear
to be within
range for
any spectrophotometer
Ɛ = extinction
coefficient
of sample
(g*cm/ l) to
D = precise.
path length
Lambert
の法則を基に計算されます。
be trustwor
thy and
For(cm)
ease of use and to avoid
manual dilution errorsC=濃度（g/L）
the NanoPhotometer ® creates a
vir tual dilution of each sample. Depending on the concenThis equation describesA=吸光度（g・cm/L）
the correlation between concentration of the sample the system applies an automatic
ε=サンプルの吸光度係数
tration, absorbance and path length. Measurements need
adjustment of the path length. The instrument always
to be within the linear range
for any spectrophotometer to
d=光路長（mm）
measures first the longer 0.67 mm path and if necessar y
be trustwor thy and precise. For ease of use and to avoid
この法則は、濃度、吸光度、光路長との相関関係を表してい
as a second step the shor ter 0.07 mm path.
manual dilution errors the NanoPhotometer ® creates a
ます。どの分光光度計でも、測定結果は、信頼性と正確性の
vir tual dilution of each sample. Depending on the concenSampl e Compression
ために、直線関係にあることが必要です。簡便性および手動
tration of the sample the system applies an automatic
In comparison to other technologies, the NanoPhotometer ®
での希釈のミス防止のため、ナノフォトメーターでは、サン
adjustment of the path length. The instrument always
uses an optical geometry similar to a microscope setup.
プルを擬似的に希釈します。サンプルの濃度に応じて光路長
measures
first the longer 0.67 mm path and if necessary
0.3 µl – 2 µl of sample are applied to the pedestal, which
を自動で調節します。ナノフォトメーターは最初に
0.67mm
as
a second step the shor ter 0.07 mm path.
acts as a “microscope slide”. The “cover glass” is a mirror
の長い光路長で測定し、必要に応じて 0.07mm の短い光路
embedded into the lid which when lowered squeezes the
Sampl
e Compression
長でも測定します。
drop between two quar tz surfaces (indicated by red lines
In comparison to other technologies, the NanoPhotometer ®
in figure 1 & 2).
uses an optical geometry similar to a microscope setup.
サンプル圧縮テクノロジー
0.3 µl – 2 µl of sample are applied to the pedestal, which
ナノフォトメーターは顕微鏡のセットアップに似ています。
acts
as a “microscope slide”. The “cover glass” is a mirror
embedded
into μL
theもしくは
lid which1.0〜2.0
when lowered
squeezes the
サンプル（0.3
μL）は顕微鏡のスライ
drop
between
two
quar
tz
surfaces
(indicated
by red
lines
mirrored
ドガラスとして機能する台座に乗せます。カバーガラスは、
します。
The NanoPhotometer ® NP80/ N60/ N50 always reads the
0.07mm 光路長
0.67 mm path length first and only if the sample is not in
mirrored
the linear range, moves the pedestal to for m the second
quar tz glass
ミラー
quar
台座 tz glass
shor ter 0.07 mm path (figure 2).
window
mirrored
quar tz glass
図 2．サンプルが２つのクォーツ表面に挟まれた図（光路長：0.07mm）
Figure 2: Sample drop squeezed between two quar tz surfaces
quar tz glass
(0.07 mm path)
window
ナノフォトメーター NP80/N60/N50 は、初めに光路長 0.67
㎜で測定します。もしサンプルが測定可能範囲を超えた場合、
台座が動き、短い光路長
0.07 ㎜で測定します。
Contained Sampl e Environment
Sample
Compression
Technology™
the
added benefit
Figure
2: Sample
drop squeezed
between twohas
quar
tz surfaces
(0.07
path)the sample in place and evaporation can be
of mm
holding
サンプルの環境
neglected. This sealed and protected microenvironment
allows reliable protein measurements and the analysis of
サンプル圧縮テクノロジーはその場所にサンプルを維持すること、
samples in volatile solvents. Even kinetic studies in small
Contained
Sample Environment
また蒸発を軽減するという利点があります。この閉鎖系の環境は、
volumes are possible with this technology.
Sample
Compression Technology™ has the added benefit
信頼性のある蛋白質測定と揮発性溶媒中のサンプル測定を可能にし
ofます。
holding the sample in place and evaporation can be
Concl usion
quar tz glass neglected.
in
figure
1
&
2).
This sealed and protected microenvironment
２つの石英表面の間にドロップを押しつぶす様に設計された、
The
patented
Sample Compression Technology™ is key to
quar tz glass allows reliable protein measurements and the analysis of
蓋に埋め込まれたミラーです。
結論
obtain
reliable
accurate
readings
from ultralow volume
window
samples in volatileand
solvents.
Even
kinetic studies
in small
0.67mm 光路長
mirrored
quar tz glass
ミラー
Figure 1: Sample drop squeezed between two quar tz surfaces
quar tz glass
台座
(0.67 mm path); light path indicated by orange arrows.
window
samples.
the with
contained
microenvironment geometr y,
サンプル圧縮テクノロジーは、超低量のサンプルで信頼性と正確性
volumes
are With
possible
this technology.
the instrument is optimized for reliable performance in all
の高い測定を可能にする鍵となります。この測定技術によって、ナ
climate zones and also is ideal for challenging studies like
Conclusion
ノフォトメーターは信頼性の高い測定を実現します。表面張力の低
low
surfaceSample
tensionCompression
proteins, volatile
solvents or
kinetics
The
patented
Technology™
is key
to
い蛋白質溶液や揮発性溶媒を含むサンプルなどの測定に理想的な装
in
a
drop.
obtain reliable and accurate readings from ultra-low volume
置です。
samples. With the contained microenvironment geometr y,
the instrument is optimized for reliable performance in all
Impl en GmbH
| E-Mail: suppor
Schatzbogen 52 | 81829 Munich | Phone: +49 89 7263718
20 | Fax:
89is7263718
[email protected]
climate zones
and+49
also
ideal for54challenging
studies
like
図１．サンプルドロップがクォーツ表面間に押しつぶされた図（光路長：
Figure 1: Sample drop squeezed between two quar tz surfaces
Copyright 2016 Implen GmbH | Version: March 2016
0.67mm）光路はオレンジ色で表示された部分。
(0.67 mm path); light path indicated by orange arrows.
Implen GmbH
Schatzbogen 52 | 81829 Munich | Phone: +49 89 7263718 20 | Fax: +49 89 7263718 54 | E-Mail: suppor [email protected]
Copyright 2016 Implen GmbH | Version: March 2016
Implen GmbH
low surface tension proteins, volatile solvents or kinetics
Seite 1
www.i m pl en.de
in a drop.
Seite 1
www.i m pl en.de
Schatzbogen 52 | 81829 Munich | Phone: +49 89 7263718 20 | Fax: +49 89 7263718 54 | E-Mail: [email protected]
Copyright 2016 Implen GmbH | Version: March 2
www.implen.de

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