マイクロビーム放射線照射法を利用した低線量（率）

Space Utiliz Res, 24 (2008)
© ISAS/JAXA 2008
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Radiation-quality-dependence in bystander cellular effects on normal human cells
induced by carbon-ion and X-ray microbeams.
Masao Suzuki1), Yoshiya Furusawa1), Chizuru Tsuruoka1), Katsumi Kobayashi2), Noriko
Usami2), Munetoshi Maeda2), Tomoo Funayama3), Tetsuya Sakashita3), Yuichiro Yokota3),
Kana Fukamoto3), Nobuyuki Hamada3, 4), Yasuhiko Kobayashi3, 4)
1) National Institute of Radiological Sciences, Inage, Chiba 263-8555
2) High Energy Accelerator Research Organization, Tsukuba, Ibaraki 305-0801
3) Japan Atomic Energy Agency, Takasaki, Gumma 370-1292
4) Gumma Universty, Maebashi, Gumma 371-8511
E-Mail: [email protected]
Abstract: We have begun to investigate cellular bystander responses in normal human
fibroblasts induced by microbeams of different raduation types. This year, we used two different
microbeams, such as carbon ions as high-LET radiations and X rays as low-LET radiations.
Carbon-ion microbeams (12C5+, 220MeV) were generated with the Takasaki Ion Accelerators for
Advanced Radiation Application (TIARA) at Japan Atomic Energy Agency. Mono-energetic
X-ray (5.35keV) microbeams were produced with the Photon Factory (PF) at High Energy
Accelerator Research Organization. We designed that only 0.2% of total cells was irradiated
with each microbeam. The percent cell survival in microbeam-irradiated dishes was ranging
from 83% to 94% for carbon ions, while around 100% for X rays. Mutation frequency for
carbon-ion-microbeam irradiated cells was about 6 times higher than that for non-irradiated
control cells, but there was no difference in mutation frequency between X-ray-microbeam
irradiated and control cells. There is clear evidence that bystander cellular effects induced by
low-dose radiations dependes on a radiation quality.
Key words; microbeam, carbon ion, synchrotron radiation, bystander effect
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1.0
0.8
0.6
0.4
0.2
0
IR
L+IR
IR
L+IR
Fig.2 Cell-killing effects in normal human fibroblasts
irradiated with either C-ion (left) or X-ray (right)
microbeam treated with (IR+L) / without (IR) a specific
inhibitor
of
gap-junction
mediated
cell-cell
communication. Results presented are the mean and
standard error of three or four independent experiments.
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60
40
20
0
Cont.
30 mm
36 mm
Fig.1 Designed micrbeam-irradiation procedure using a
256 (16 x 16)-cross-stripe method. A microbeam dish was
constructed with an aluminum ring of 36 mm diameter
and attached 7.5 m-thick polyimide film on the bottom
of the ring. Eight ions of carbon and 0.4Gy of X rays were
irradiated within each 20m x 20m beam size.
IR
L+Cont. L+IR
Cont.
IR L+Cont. L+IR
Fig.3 Mutation induction in normal human fibroblasts
irradiated with either C-ion (left) or X-ray (right)
microbeam treated with (IR+L) / without (IR) a specific
inhibitor
of
gap-junction
mediated
cell-cell
communication. Cont. means non-irradiated control cells.
Results presented are the mean and standard error of three
or four independent experiments.
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