Chemi-LumiOneL

149
ႆήਤዓ଺᧓ƕࢼஹԼƷ4̿ƴࡨᧈ
Chemi-Lumi One L
䊕䊦䉥䉨䉲䉻䊷䉷㜞ᗵᐲᬌ಴↪ൻቇ⊒శ⹜⮎㩷㪚㪿㪼㫄㫀㪄㪣㫌㫄㫀㩷㪦㫅㪼㩷㪣䈱⵾ຠ⇟ภ䉕ᄌᦝ䈜䉎䈫䈫䉅䈮ផᅑ䊒䊨䊃䉮
䊷䊦䉕ᄌᦝ䈚䉁䈚䈢䇯
ཎųᧈ
ႆήƷਤዓࣱ
Chemi-Lumi One Ʒ4̿ˌɥᲣ
Ū இ᭗ႆήࢍࡇLJưᡮǍƔƴПᢋ
Ū ႆήࢍࡇƸChemi-Lumi One Ʒኖ1.5̿
Ū ႆήਤዓ଺᧓ƕࡨᧈᲢࢼஹԼ
⊒శᜬ⛯↪䈮⿥㜞⚐ᐲ⹜⮎ᷝട೷䉕ട䈋䉎䈖䈫䈪䇮⊒శ䈱ᜬ⛯ᕈ䉕ะ਄䈚䈩䈇䉁䈜䇯
㐳ᤨ㑆㔺శ䈮䉋䉎↹௝䈱㞲᣿ൻ䇮ਁ䈏৻䈱⃻௝ᄬᢌᤨ䈱㔺శ䈱䉇䉍⋥䈚䈏น⢻䈪䈜䇯
ႆήࢍࡇƱਤዓࣱ
min
Chemi-Lumi One L ᓥ᧪ຠ㩷Chemi-Lumi One
A␠
B␠
5
15
䉺䊮䊌䉪⾰䊙䊷䉦䊷㩷 䋨䊎䉥䉼䊮ᮡ⼂䋩䈱㪮㪼㫊㫋㪼㫉㫅㩷 㪙㫃㫆㫋㫋㫀㫅㪾
䉕ⴕ䈇䇮䊑䊨䉾䉨䊮䉫䊪䊮䉕↪䈇䈩䊑䊨䉾䉨䊮䉫ᓟ䇮䉴䊃
䊧䊒䊃㪘㪙㪚䊕䊦䉥䉨䉲䉻䊷䉷䉨䉾䊃䉕↪䈇䈩䊕䊦䉥䉨䉲
䉻䊷䉷䉕⚿ว䈘䈞䇮ฦ⒳ൻቇ⊒శ䉨䉾䊃䈮䈩ᬌ಴䉕ⴕ䈇
䉁䈚䈢䇯ᤨ㑆䈲⊒శ෻ᔕ㐿ᆎ䈎䉌䈱⚻ㆊᤨ㑆䉕⴫䈚䉁䈜䇯
䋨Ꮧ㉼♽೉䋺Ꮐ䈎䉌䇮㪋㪇㪇䇮㪉㪇㪇䇮㪈㪇㪇୚Ꮧ㉼䋩
30
60
90
120
̅ဇ̊
Chemi-Lumi One 䇮Chemi-Lumi One L䈫䉅䈮ห৻䉭䊦䇮ห৻᛫૕䉕૶↪䈚䈢䊜䊮䊑䊧䊮䉕ಾ䉍ಽ
䈔䈩䇮⹏ଔ䈚䉁䈚䈢䇯
䋨㪘䋩⊒శ෻ᔕ㐿ᆎ⋥ᓟ䈮㪊㪇⑽㔺శ
Chemi-Lumi One
1 2
3
䋨㪙䋩⊒శ෻ᔕ㐿ᆎ䈎䉌⚂㪊㪇ಽᓟ䈮㪊㪇⑽㔺శ
Chemi-Lumi One L
1
2
3
Chemi-Lumi One
1 2
3
⋡⊛࠲ࡦࡄࠢ⾰ψ
⋡⊛࠲ࡦࡄࠢ⾰ψ
GAPDHψ
GAPDHψ
Chemi-Lumi One L
1
2
3
䉰䊮䊒䊦䋺䊍䊃㵘ೋઍၭ㙃⢄⚦⢩
⚿ᨐ䋺Chemi-Lumi One L䈱䈾䈉䈏⊒శᒝᐲ䇮ᜬ⛯ᕈ౒䈮ఝ䉏䈩䈇䉎䈖䈫䈏⏕⹺䈪䈐䉁䈚䈢䇯
䊂䊷䉺䊷䈗ឭଏ䋺ᄢ㒋ᄢቇ㩷⮎ቇ⎇ⓥ⑼㩷⥃ᐥ⮎ല⸃ᨆቇ
‫୼٭‬ȗȭȈdzȸȫ
䋨એਅ䈱⸥タ䈲䉡䉢䉴䉺䊮䊑䊨䉾䊁䉞䊮䉫䈱৻଀䈪䈜䈱䈪䇮ታ㛎♽䈮ᓥ䈦䈩૶↪᧦ઙ䊶⹜⮎䉕ᄌᦝ䈜䉎੐䈏
㩷น⢻䈪䈜䇯䋩
R
1䋮䉰䊮䊒䊦䇮䉺䊮䊌䉪⾰䊙䊷䉦䊷䉕㔚᳇ᵒേᓟ䇮䉭䊦䈎䉌⤑䈮ォ౮䈚䇮0.05%Tween20㵘฽᦭TBS䋨t-TBS䋩䉕
㩷㩷㩷㩷㩷㩷㩷↪䈇䈩⤑䉕ᵞᵺ䈚䉁䈜䇯䋨5ಽ㑆㬍2࿁䋩
2䋮䊑䊨䉾䉨䊮䉫䊪䊮䋨⵾ຠ⇟ภ䋺03953䋩 䉕↪䈇䈩ቶ᷷䈪45ಽ㑆䊑䊨䉾䉨䊮䉫ಣℂ䉕ⴕ䈇䉁䈜䇯
3䋮t-TBS䉕↪䈇䈩⤑䉕ᵞᵺ䈚䉁䈜䇯䋨5ಽ㑆㬍2࿁䋩
4䋮1ᰴ᛫૕䉕෻ᔕ䈘䈞䉁䈜䇯䋨ቶ᷷䇮1ᤨ㑆䋩
5䋮t-TBS䉕↪䈇䈩⤑䉕ᵞᵺ䈚䉁䈜䇯䋨5ಽ㑆㬍2࿁䋩
6䋮HRPᮡ⼂᛫૕䋨2ᰴ᛫૕䋩䉕෻ᔕ䈘䈞䉁䈜䇯䋨ቶ᷷䇮1ᤨ㑆䋩
7䋮t-TBS䉕↪䈇䈩⤑䉕ᵞᵺ䈚䉁䈜䇯䋨5ಽ㑆㬍4࿁䋩
8䋮Workingṁᶧ䉕⺞⵾䈚䉁䈜䇯
R
9䋮䊑䊨䉾䊁䉞䊮䉫⤑䈱┵䉕䉨䊛䊪䉟䊒㵘䈭䈬䈮ធ⸅䈘䈞䈩䇮ᵞᵺᓟ䈱ᱷሽᶧ䉕චಽ䈮㒰෰䈚䉁䈜䇯
R
10䋮䉰䊤䊮䊤䉾䊒㵘䈭䈬䈱䊒䊤䉴䉼䉾䉪⵾䊤䉾䊒䊐䉞䊦䊛䉕᧍਄䈮ᢝ䈐䇮䈠䈱਄䈮䉺䊮䊌䉪⾰ๆ⌕஥䈏਄஥䈮
㩷㩷㩷㩷㩷㩷㩷䈭䉎䉋䈉䈮䊑䊨䉾䊁䉞䊮䉫⤑䉕⟎䈐䉁䈜䇯਄䈎䉌Workingṁᶧ䉕䉉䈦䈒䉍䈫ᵈ䈇䈪⤑䉕ᶐ䈚䇮1ಽ㑆෻ᔕ䈘䈞䉁䈜䇯
R
11䋮⤑䈱┵䉕䉨䊛䊪䉟䊒㵘䈭䈬䈮ធ⸅䈘䈞䈩䇮૛ಽ䈭ᶧ䉕ข䉍㒰䈐䇮⤑䉕චಽ䈮൮䉄䉎⒟ᐲ䈱ᄢ䈐䈘䈱ᣂ䈚䈇
㩷㩷㩷㩷㩷㩷㩷㩷䊒䊤䉴䉼䉾䉪⵾䊤䉾䊒䊐䉞䊦䊛䈮䉺䊮䊌䉪⾰ๆ⌕஥䈱㕙䈏ਅ஥䈮䈭䉎䉋䈉䈮䊑䊨䉾䊁䉞䊮䉫⤑䉕⟎䈐䉁䈜䇯
㩷㩷㩷㩷㩷㩷㩷㩷䋨䈖䈱ᤨ䇮⤑䈫䊤䉾䊒䊐䉞䊦䊛䈱㑆䈮᳇ᵃ䈏౉䉌䈭䈇䉋䈉䇮䉁䈢䊤䉾䊒䊐䉞䊦䊛䈱᛬䉏䉇䉲䊪䈏⴫㕙䈮಴᧪䈭
㩷㩷㩷㩷㩷㩷㩷㩷㩷䈇䉋䈉䈮ᵈᗧ䈚䈩䈒䈣䈘䈇䇯䋩
12䋮䊤䉾䊒䊐䉞䊦䊛䈪䊑䊨䉾䊁䉞䊮䉫⤑䉕൮䉂䇮䉺䊮䊌䉪⾰ๆ⌕஥䈱㕙䉕਄䈮䈚䈩䊐䉞䊦䊛䉦䉶䉾䊃䈮⟎䈐䇮
㩷㩷㩷㩷㩷㩷㩷㩷䊐䉞䊦䊛䉦䉶䉾䊃䉕ᥧቶ䈮⟎䈐䉁䈜䇯
13䋮ᥧቶౝ䈪䇮䊤䉾䊒䊐䉞䊦䊛䈪൮䉖䈣䊑䊨䉾䊁䉞䊮䉫⤑䈱਄䈮䌘✢䊐䉞䊦䊛䉕⟎䈐䇮䊐䉞䊦䊛䉦䉶䉾䊃䉕㐽䉄䇮
3ಽ㑆ᗵశ䈘䈞䉁䈜䇯
14䋮䌘✢䊐䉞䊦䊛䉕⃻௝ᶧ䈮ẃ䈔䇮௝䈏⏕⹺䈪䈐䉎䉁䈪⃻௝䈚䉁䈜䇯
15䋮䌘✢䊐䉞䊦䊛䉕ቯ⌕ᶧ䈮ẃ䈔䇮௝䉕ቯ⌕䈘䈞䉁䈜䇯
16䋮䌘✢䊐䉞䊦䊛䉕ᥧቶ䈎䉌಴䈚䇮⚿ᨐ䉕⏕⹺䈚䉁䈜䇯
̅ဇɥƷදॖ
䊶㩷䊑䊨䉾䊁䉞䊮䉫䈮䈲㪧㪭㪛㪝⤑䈱૶↪䉕ផᅑ䈚䉁䈜䇯䈭䈍䇮䊆䊃䊨䉶䊦䊨䊷䉴⤑䉕૶↪䈜䉎䈫ᗵᐲ䈏ૐਅ䈜䉎
႐ว䈏䈅䉍䉁䈜䈱䈪䈗ᵈᗧ㗿䈇䉁䈜䇯
䊶㩷ᧄ䉨䉾䊃䈮䈲⣣㘩ᕈ䈱‛⾰䈏฽䉁䉏䈩䈇䉁䈜䇯䈗૶↪䈱㓙䈮䈲ᚻⴼ䇮㒐⼔⌒㏜䈭䈬䉕⌕↪䈚䈩䈒䈣䈘䈇䇯
䊶㩷⹜⮎䉕⺞⵾䈜䉎㓙䈮䈲䇮ᔅ䈝ቶ᷷䈮ᚯ䈚䈩䈎䉌↉䉕㐿ኽ䈚䈩䈒䈣䈘䈇䇯
䊶㩷䊑䊨䉾䊁䉞䊮䉫⤑䈲ᚻⴼ⌕↪䉁䈢䈲䊏䊮䉶䉾䊃䉕↪䈇䈩ข䉍ᛒ䈦䈩䈒䈣䈘䈇䇯
䊶㩷⤑䉕䊤䉾䊒䊐䉞䊦䊛䈪൮䉃㓙䇮⤑䈫䊤䉾䊒䊐䉞䊦䊛䈱㑆䈮᳇ᵃ䈏౉䉌䈭䈇䉋䈉䇮䉁䈢䊤䉾䊒䊐䉞䊦䊛䈱᛬䉏䉇
䉲䊪䈏⴫㕙䈮಴᧪䈭䈇䉋䈉䈮ᵈᗧ䈚䈩䈒䈣䈘䈇䇯
̖఍ᘙ
ᙌԼӸ
ᙹ఍
ᝪඥ
ᙌԼဪӭ
ܾ᣽
̖఍
SP
಄⬿
ᣥ06567-20ųᣂ07880-70
1 kit
25,000
Chemi-Lumi One L
᧙ᡲᙌԼ
ᙌԼӸ
Pre-stained Protein Markers(Broad Range)
Blocking One
Blocking One-P
WB Stripping Solution
WB Stripping Solution Strong
ᙹ఍
ᝪඥ
ᙌԼဪӭ
ܾ᣽
̖఍
SP
SP
SP
SP
SP
಄ಓ
಄⬿
಄⬿
಄⬿
಄⬿
02525-35
03953-95
05999-84
05364-55
05677-65
500Ǵl
500 ml
200 ml
500 ml
500 ml
13,000
6,000
4,000
15,000
18,000
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