RNA 生物学分野セミナー（第3回）連絡先：メディカル情報生命専攻 RNA

RNA 生物学分野セミナー（第３回）
Repertoire, biogenesis, and function of tRNA-derived non-coding RNAs
Dr. Yohei Kirino(桐野
陽平博士)
Computational Medicine Center, Department of Biochemistry and Molecular Biology,
Sidney Kimmel Medical College, Thomas Jefferson University
Although transfer RNAs (tRNAs) are best known as adapter components of
translational machinery, recent studies suggest that tRNAs are not always
end products but can further serve as a source for short non-coding RNAs
(ncRNAs). In many organisms, various tRNA-derived ncRNA species are
produced from mature tRNAs or their precursor transcripts as functional
molecules involved in many biological processes beyond translation. Our
recent studies have identified a novel type of tRNA-derived ncRNAs, termed
Sex HOrmone-dependent TRNA-derived RNAs (SHOT-RNAs), which is specifically
and abundantly expressed in estrogen receptor (ER)-positive breast cancer
and androgen receptor (AR)-positive prostate cancer. SHOT-RNAs are
produced from aminoacylated mature tRNAs by angiogenin-mediated anticodon
cleavage, which is promoted by sex hormones (estrogen/androgen) and their
receptors (ER/AR). Importantly, SHOT-RNAs boost cell proliferation by
promoting cell cycle progression, designating SHOT-RNAs as functional RNAs
potentially involved in tumorigenesis and tumor growth. Therefore, our
results have unveiled a novel tRNA-engaged pathway in cancer biology. In
the course of these analyses, we have developed several biochemical methods
(e.g., cP-RNA-seq, FL-PCR, Db-PCR, and YAMAT-seq) which enable specific
quantification and sequencing of tRNAs and tRNA-derived ncRNAs. The
efficiency, specificity, and applicability of these methods will be
discussed as well.
2016 年７月７日（木）13:00 - 14:45 生命棟
連絡先：メディカル情報生命専攻
[email protected]
地下
セミナー室
RNA 生物学分野富田耕造
内線 63611

Download Report