- Invitation - Seminar “Measurement Systems” Fast contactless three

FAKULTÄT ELEKTROTECHNIK UND INFORMATIONSTECHNIK
Institut für Grundlagen der Elektrotechnik und Elektronik
Technische Universität Dresden, 01062 Dresden
Prof. Dr.-Ing. habil.
Jürgen Czarske
Professur für Mess- und Sensorsystemtechnik
Bearbeiterin: Cathleen John
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- Invitation Seminar “Measurement Systems”
Fast contactless three-dimensional and multimodal zebrafish imaging
Dr. Jan Huisken, MPI-CBG, Dresden
Abstract:
Fluorescence light-sheet microscopy such as Selective Plane Illumination Microscopy (SPIM) [Huisken et al., Science, 2004] has proven to be
a powerful tool to image developmental processes in vivo with fast high-resolution optical sectioning over large tissue volumes [Weber et
al., Methods Cell Biol., 2014]. Recently, we have shown that even the intact beating zebrafish heart can be reconstructed at 400 frames/s
[Mickoleit et al., Nat. Methods, 2014]. One key development for these applications has been the integration of fast scanners and liquid lenses
in our light sheet microscope setups [Fahrbach et al., Opt. Express, 2013]. Three-dimensional volumes are now imaged almost instantaneously with volume rates of up to 120 volumes/s. This technology does not require any scanning of the sample anymore: it is essentially
contactless and motionless. Without modifying any component of a typical SPIM setup, we now managed to perform a multimodal acquisition that integrates the high-resolution SPIM fluorescence data with optical tomography, purely based on bright-field contrast [Bassi et al.,
Development, 2015].
CV:
Dr. Jan Huisken received his PhD in Physics from the University of Freiburg in 2004. The main achievement during his thesis has been the
development of Selective Plane Illumination Microscopy (SPIM) in the lab of Ernst Stelzer at the European Molecular Biology Laboratory
(EMBL) in Heidelberg. During a short postdoctoral stay in the lab of Joachim Wittbrodt (also at EMBL) he explored applications in developmental biology and got interested in imaging medaka and zebrafish. To further improve and apply his novel technology he moved to the
University of California in San Francisco (UCSF) and joined the lab of Didier Stainier in 2005. Dr. Huisken is currently a Max Planck Research
Group Leader (MPRGL) at the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden (MPI-CBG) since 2010.
Time: Thursday, April 28, 2016, 11:10 am
Place: BAR 213 (Barkhausenbau)
We would be pleased to welcome you as guest in our seminar.
Prof. Jürgen Czarske
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