RobertTampé
RobertTampé,Ph.D.
Goethe-UniversityFrankfurt
ProfessorofBiochemistry
[email protected]
InstituteofBiochemistry,Biocenter
Phone: +49-(0)69-79829475
Max-von-Laue-Str.9
Fax:
+49-(0)69-79829495
60438Frankfurt/M.,Germany
www.biochem.uni-frankfurt.de
Education
1981-87
Diploma(Chemistry),TUDarmstadt,Germany
1987-89
PhD(Biochemistry),TUDarmstadt,Germany
1990-91
PostdoctoralFellowwithProf.Dr.H.M.McConnell,StanfordUniversity,USA
EmploymentandResearchExperience
2010-
HonoraryGuestProfessoratiCeMS,KyotoUniversity,Japan
2009-10
VisitingProfessor,UniversityofCaliforniaSanFrancisco(UCSF),USA
2001-
FullProfessor,Director,Inst.Biochemistry,Biocenter,Goethe-UniversityFrankfurt
1998-01
FullProfessor&Director,PhysiologicalChemistry,Medicine,UniversityMarburg
1996-98
AssistantProfessorforBiochemistryandBiophysics,TUMunich,Germany
1996
HabilitationinBiochemistry,TUMunich
1992-98
GroupLeader,Max-Planck-InstituteofBiochemistry,Martinsried/Munich
1992-98
ResearchGroupLeader,BiophysicsDepartment,TUMunich
HonorsandAwards
2014-
LiaisonOfficer,GermanResearchFoundation(DFG),Goethe-UniversityFrankfurt
2010-
Chair,PaulEhrlichYoungInvestigatorPrize,PaulEhrlichFoundation
2012
ExcellenceinTeachingBiochemistry
2010&2011 Award:BestTeachinginBiochemistry
1996-98
HeisenbergFellow,GermanResearchFoundation
1989-91
PostdoctoralFellowshipofDFGandMax-KadeFoundation(NewYork)
1989
Ph.D.inBiochemistry,summacumlaude
1987-89
DoctoralFellowship,FondsderChemischenIndustrie(FCI)
1987
DiplomainChemistry,summacumlaude
1985
AntonKellerPrizeinChemistry
1984-87
Fellowship,StudienstiftungdesDeutschenVolkes
1983-87
Fellowship,Cusanuswerk
Appointments
2008-
Director,CollaborativeResearchCenter(CRC807)TransportandCommunication
acrossBiologicalMembranes–www.sfb807.de
2007-
BoardofDirectors&PrincipleInvestigatoroftheClusterofExcellenceFrankfurt–
MacromolecularComplexes–www.cef-mc.de
2008-11
ScienceReviewPanel,GermanResearchFoundation(BasicResearchinMedicine)
2007-10
Coordinator,Biomembranes,GermanSocietyforBiochemistryandMolecularBiology
2005-10
Coordinator,EuropeanMembraneBiologyNetwork(EMBN-Train)
2003-08
EditorialBoardMember,TheJournalofBiologicalChemistry(ASBMB)
2003-07
Director,CollaborativeResearchCenter(CRC628)FunctionalMembraneProteomics
2001-03
EditorofFaculty1000
2001
Co-founderoftheCenterforMembraneProteomics(CMP),Frankfurta.M.
ResearchInterests
StructureandFunctionofABCProteins
IntracellularTransportandMaturationofProteins
MechanismofAntigenProcessingandViralEscape
MacromolecularRNA-ProteinComplexes
Chemical&SyntheticBiology,Nanobiotechnology
-1-
RobertTampé
SelectedPublications:
outof247publications(8628citations;33.44av.cit.;h-Index50;ISI,Jan2016)
1)
KimJM,Wu,S,TomasiakT,MergelC,WinterMN,StillerS,Robles-ColmanaresY,StroudRM*,
TampéR*,CraikCS*,ChengY*(2015)Subnanometre-resolutionelectroncryomicroscopy
structureofaheterodimericABCexporter.Nature517,396-400.(*sharedcorresponding
authorship)http://dx.doi.org/10.1038/nature13872
2)
GrossmannN,VakkasogluAS,HulpkeS,AbeleR,GaudetR,TampéR(2014)Mechanistic
determinantsofthedirectionalityandenergeticsofactiveexportbyaheterodimericABC
transporter.NatureCommun5,5419.http://dx.doiorg/10.1038/ncomms6419
3)
GatterdamV,RamadassR,StoessT,FichteMAH,WachtveitlJ,HeckelA,TampéR(2014)
Three-dimensionalproteinnetworksassembledbytwo-photonactivation.AngewChemIntEd
53,5680-4.http://dx.doi.org/10.1002/anie.201309930
4)
WeiR,GatterdamV,WienekeR,TampéR*,RantU*(2012)Stochasticsensingofproteinswith
receptor-modifiedsolid-statenanopores.NatureNanotechn7,257-63.(*shared
correspondingauthorship)http://dx.doi.org/10.1038/nnano.2012.24
5)
BarthelmeD,DinkelakerS,AlbersSV,LondeiP,ErmlerU,TampéR(2011)Ribosomerecycling
dependsonamechanisticlinkbetweentheFeSclusterdomainandaconformationalswitchof
thetwin–ATPaseABCE1.ProcNatlAcadSciUSA108,3228-33.
http://dx.doi.org/10.1073/pnas.1015953108
6)
HergetM,BaldaufC,SchölzS,ParcejD,WiesmüllerKH,TampéR,AbeleR,BordignonE(2011)
ConformationofantigenicpeptidesboundtotheABCtransporterTAP.ProcNatlAcadSciUSA
108,1349-54.http://dx.doi.org/10.1073/pnas.1012355108
7)
GrunwaldC,SchulzeK,ReichelA,WeissVU,PiehlerJ,WiesmüllerKH,TampéR(2010)Insitu
assemblyofmacromolecularcomplexestriggeredbylight.ProcNatlAcadSciUSA107,6146–
6151.http://dx.doi.org/10.1073/pnas.0912617107
8)
BurgdorfS,SchölzC,KautzA,TampéR,KurtsC(2008)Spatialandmechanisticseparationof
cross-presentationandendogenousantigenpresentation.NatureImmunology9,558-66.
http://dx.doi.org/10.1038/ni.1601
9)
TinazliA,PiehlerJ,BeuttlerM,GuckenbergerR,TampéR(2007)Nativeprotein
nanolithographythatcanwrite,read,anderase.NatureNanotechnolgy2,220-225.
http://dx.doi.org/10.1038/nnano.2007.63
10)
HutschenreiterS,TinazliA,ModelK,TampéR(2004)Two-substrateassociationwiththe
proteasomeatsingle-moleculelevel.EMBOJ23,2488-97.
http://dx.doi.org/10.1038/sj.emboj.7600262
11)
GorbulevS,AbeleR,TampéR(2001)Allostericcrosstalkbetweenpeptide-binding,transport,
andATPhydrolysisoftheABCtransporterTAP.ProcNatlAcadSciUSA98,3732-37.
http://dx.doi.org/10.1073/pnas.061467898
12)
UebelS,KraasW,KienleS,WiesmüllerKH,JungG,TampéR(1997)Recognitionprincipleof
TAPasdisclosedbycombinatorialpeptidelibraries.
ProcNatlAcadSciUSA94,8976-81.
13)
AhnK,MeyerTH,UebelS,SempéP,DjaballahH,PetersonPA,FrühK,TampéR(1996)
MolecularmechanismandspeciesspecificityofTAPinhibitionbyHerpessimplexvirusprotein
ICP47.EMBOJ15,3247-55.
14)
FrühK,AhnK,DjaballahH,SempéP,vanEndertPM,TampéR,PetersonPA,YangY(1995)A
viralinhibitorofpeptidetransportersforantigenpresentation.Nature375,415-8.
15)
TampéR,ClarkBR,McConnellHM(1991)Energytransferbetweentwopeptidesboundtoone
MHCclassIImolecule.Science254,87-9.
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RobertTampé
Publications(peerreviewed):
PubMed:http://www.ncbi.nlm.nih.gov/pubmed/?term=Tampe+R
ResearchGate:https://www.researchgate.net/profile/Robert_Tampe
1)
KollmannspergerA,ShareiA,RaulfA,HeilemannM,LangerR,JensenKF,WienekeR,TampéR
(2016)Live-cellproteinlabellingwithnanometreprecisionbycellsqueezing.NatureCommun
7:10372.http://dx.doi.org/10.1038/ncomms10372
2)
BranerM,KollmannspergerA,WienekeR,TampéR(2016)Traceless’TracingofProteins–
High-affinitytrans-splicingdirectedbyaminimalinteractionpair.ChemSci,inpress.
http://dx.doi.org/10.1039/C5SC02936H
3)
HeiseR,AmannPM,EnsslenS,MarquardtY,JoussenS,JohannD,AbeleR,PlewniaG,TampéR,
MerkHF,HermannsHM,BaronJM(2016)Interferonalphasignallinganditsrelevanceforthe
upregulatoryeffectoftransporterproteinsassociatedwithantigenprocessing(TAP)in
patientswithmalignantmelanoma.PLoSOne11,e0146325.
http://dx.doi.org/10.1371/journal.pone.0146325
4)
FisetteO,WingbermühleS,TampéR,SchäferLV(2015)Molecularmechanismofpeptide
editinginthetapasin-MHCIcomplex.SciRep5,19085.http://dx.doi.org/10.1038/srep19085
5)
BleesA,ReichelK,TrowitzschS,FisetteO,BockC,AbeleR,HummerG,SchäferLV,TampéR
(2015)AssemblyoftheMHCIpeptide-loadingcomplexdeterminedbyaconservedioniclockswitch.SciRep5,17341.http://dx.doi.org/10.1038/srep17341
6)
UrbanM,TampéR(2015)Membranesonnanoporesformultiplexedsingle-transporter
analyses.MicrochimicaActa,inpress.
7)
PfreundschuhM,AlsteensD,WienekeR,ZhangC,CoughlinS,TampéR,KobilkaB,MüllerD
(2015)Identifyingandquantifyingtwoligand-bindingsiteswhileimagingnativehuman
membranereceptorsbyAFM.NatureCommun6,8857.
http://dx.doi.org/10.1038/ncomms9857
8)
FleischmannG,FisetteO,ThomasC,WienekeR,TumulkaF,SchneeweissC,Springer,S.
SchäferLV,TampéR(2015)Mechanisticbasisforepitopeproofreadinginthepeptide-loading
complex.JImmunol195,4503-13.http://dx.doi.org/10.4049/jimmunol.1501515
–HighlightedbyCuttingEdgeArticle:http://dx.doi.org/10.4049/jimmunol.1590020
9)
UrbanM,VorderBrüggenM,TampéR(2015)Membranetransportprocessesanalyzedbya
highlyparallelnanoporechipsystematsingleproteinresolution.JoVE,inpress.
10)
WienekeR,RaulfA,KollmannspergerA,HeilemannM,TampéR(2015)SLAP:Smalllabeling
pairforsingle-moleculesuper-resolutionimaging.AngewChemIntEd54,10216-9.
http://dx.doi.org/10.1002/anie.201503215–HighlightedbyFrontCoverPage
11)
Strale,PO,DuchesneL,PeyretG,MontelL,NguyenT,PngE,TampéR,TroyanovskyS,HénonS,
LadouxB,MègeRM(2015)Theformationoforderednano-clusterscontrolscadherin
anchoringtoactinandcell-cellcontactfluidity.JCellBiol210,333-346.
http://dx.doi.org/10.1083/jcb.201410111
12)
KohoT,IhalainenTO,StarkM,Uusi-KerttulaH,WienekeR,RahikainenR,BlazevicV,
MarjomäkiV,TampéR,KulomaaMS,HytönenVP(2015)His-taggednorovirus-likeparticles:A
versatileplatformforcellulardeliveryandsurfacedisplay.EurJPharmBiopharmS0939-6411,
00290-8.http://dx.doi.org/10.1016/j.ejpb.2015.07.002
13)
EggenspergerS,TampéR(2015)Thetransporterassociatedwithantigenprocessing–akey
playerinadaptiveimmunity.BiolChem396,1059-72.http://dx.doi.org/10.1515/hsz-20140320
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14)
TrowitzschS,TampéR(2015)Multicolorfluorescence-basedscreeningtowardstructural
analysisofmultiproteinmembranecomplexes.MethodsEnzymol557,3-26.
http://dx.doi.org/10.1016/bs.mie.2014.11.043
15)
BecharaC,NöllA,Morgner,N,Degiacomi,MT,TampéR*,RobinsonCV*(2015)Asubsetof
annularlipidsislinkedtotheflippaseactivityofanABCtransporter.NatureChemistry7,25562.(*sharedcorrespondingauthorship)http://dx.doi.org/10.1038/nchem.2172
16)
KimJM,Wu,S,TomasiakT,MergelC,WinterMN,StillerS,Robles-ColmanaresY,StroudRM*,
TampéR*,CraikCS*,ChengY*(2015)Subnanometre-resolutionelectroncryomicroscopy
structureofaheterodimericABCexporter.Nature517,396-400.(*sharedcorresponding
authorship)http://dx.doi.org/10.1038/nature13872
17)
ZollmannT,MoisetG,TumulkaF,TampéR,PoolmanB,AbeleR(2015)Singleliposome
analysisofpeptidetranslocationbytheABCtransporterTAPL.ProcNatlAcadSciUSA112,
2046-51.http://dx.doi.org/10.1073/pnas.1418100112
18)
FischbachH,DöringM,NiklesD,LehnertE,BaldaufC,KalinkeU,TampéR(2015)UltrasensitivequantificationofTAP-dependentantigencompartmentalizationinscarceprimary
immunecellsubsets.NatureCommun6,6199.http://dx.doi.org/10.1038/ncomms7199
19)
LinJ,EggenspergerS,HankS,WienekeR,MayerhoferPU,TampéR(2014)Anegativefeedback
modulatorofantigenprocessingevolvedfromaframeshiftinthecowpoxvirusgenome.
PLoSPathogens10,e1004554.http://dx.doi.org/10.1371/journal.ppat.1004554
20)
GrossmannN,VakkasogluAS,HulpkeS,AbeleR,GaudetR,TampéR(2014)Mechanistic
determinantsofthedirectionalityandenergeticsofactiveexportbyaheterodimericABC
transporter.NatureCommun5,5419.http://dx.doi.org/10.1038/ncomms6419
21)
HinzA,JedamzickJ,HerbringV,FischbachH,HartmannJ,ParcejD,KochJ,TampéR(2014)
AssemblyandfunctionoftheMHCIpeptide-loadingcomplexareconservedacrossjawed
vertebrates.JBiolChem289,33109-17.http://dx.doi.org/10.1074/jbc.M114.609263
22)
EggenspergerS,FisetteO,ParcejD,SchäferVL,TampéR(2014)Anannularlipidbeltis
essentialforallostericcouplingandviralinhibitionoftheantigentranslocationcomplexTAP.
JBiolChem289,33098-108.http://dx.doi.org/10.1074/jbc.M114.592832
23)
SeyfferF,TampéR(2014)ABCtransportersinadaptiveimmunity.BiophysBiochemActa1850,
449-460.http://dx.doi.org/10.1016/j.bbagen.2014.05.022
24)
WienekeR,LabòriaN,RajanM,KollmannspergerA,NataleF,CardosoMC,TampéR(2014)
Live-celltargetingofHis-taggedproteinsbymultivalentN-nitrilotriaceticacidcarrier
complexes.JAmChemSoc136,13975-8.http://dx.doi.org/10.1021/ja5063357
25)
MayerhoferPU,TampéR(2014)Antigentranslocationmachineriesinadaptiveimmunityand
viralimmuneevasion.JMolBiol427,1102-18.http://dx.doi.org/10.1016/j.jmb.2014.09.006
26)
Nair-GuptaP,BaccariniA,TungN,SeyfferF,FloreyO,Huang,Y,BanerjeeM,OverholtzerM,
RochePA,TampéR,BrownDB,AmsenD,WhiteheartSW,BlanderJM(2014)TLRsignals
inducephagosomalMHC-Ideliveryfromendosomalrecyclingcompartmenttoallowcrosspresentation.Cell185,506-514.http://dx.doi.org/10.1016/j.cell.2014.04.054
27)
ArnoldP,DeckerTM,MarklJ,GatterdamV,TampéR,HimmelsP,WeißS,BartholomäusP,
DietrichU,DürrR(2014)Antigenicand3DstructuralcharacterizationofsolubleX4HIV-1Env
trimersandanR5V3exchangemutant.Retrovirology11,42.http://dx.doi.org/10.1186/17424690-11-42
28)
GatterdamV,RamadassR,StoessT,FichteMAH,WachtveitlJ,HeckelA,TampéR(2014)
Three-dimensionalproteinnetworksassembledbytwo-photonactivation.
AngewChemIntEd53,5680-4.http://dx.doi.org/10.1002/anie.201309930
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RobertTampé
29)
UrbanM,KleefenA,MukherjeeN,SeelheimP,WindschieglB,vonderBrüggenM,KocerA,
TampéR(2014)Highlyparalleltransportrecordingsonmembrane-on-nanoporechipsatsingle
moleculeresolution.NanoLett14,1674-80.http://dx.doi.org/10.1021/nl5002873
30)
HulpkeS&TampéR(2013)TheMHCIloadingcomplex:amultitaskingmachineryinadaptive
immunity.TrendsBiochemSci38,412-420.http://dx.doi.org/10.1016/j.tibs.2013.06.003
31)
RanglM,EbnerA,YamadaJ,RanklC,TampéR,GruberHJ,RexbachM,HinterdorferP(2013)
Single-moleculeanalysisoftherecognitionforcesunderlyingnucleo-cytoplasmictransport.
AngewChemIntEd52,10356-9.http://dx.doi.org/10.1002/anie.201305359
32)
PollheimerP,TaskinenB,ScherflerA,GusenkovS,CreusM,WiesauerP,ZaunerD,Schöfberger
W,SchwarzingerC,EbnerA,TampéR,StutzH,HytönenVP,GruberHJ(2013)Reversible
biofunctionalizationofsurfaceswithaswitchablemutantofavidin.BioconjChem24,1656-68.
http://dx.doi.org/10.1021/bc400087e
33)
BaldaufC,SchulzeK,LuedersP,BordignonE,TampéR(2013)Distancemeasurementsin
macromolecularproteincomplexesbyanovelsite-directedspin-labelingstrategy.
Chemistry19,13714-9.http://dx.doi.org/10.1002/chem.201301921
34)
ParcejD,GuntrumR,SchmidtS,HinzA&TampéR(2013)Multicolourfluorescence-detection
size-exclusionchromatographyforstructuralgenomicsofmembranemultiproteincomplexes.
PLOSOne8,e67112.http://dx.doi.org/10.1371/journal.pone.0067112
35)
NürenbergE&TampéR(2013)Tyinguplooseends:ribosomerecyclingineukaryotesand
archaea.TrendsBiochemSci38,64-74.http://dx.doi.org/10.1016/j.tibs.2012.11.003
36)
LaboriaN,WienekeR&TampéR(2013)Nanomolarinteractionandin-situassemblyof
proteinsinfourdimensionscontrolledbylight.AngewChemIntEd,52,848-53.
http://dx.doi.org/10.1002/anie.201206698
37)
HornerA,GoetzF,TampéR,KlussmannE,PohlP(2012)MechanismfortargetingtheA-kinase
anchoringproteinAKAP18tothemembrane.JBiolChem287,42495-501.
http://dx.doi.org/10.1074/jbc.M112.414946
38)
HulpkeS,BaldaufC,TampéR(2012)MoleculararchitectureoftheMHCIpeptide-loading
complex–onetapasinmoleculeisessentialandsufficientforantigenprocessing.
FASEBJ26,5071-80.http://dx.doi.org/10.1096/fj.12-217489
39)
RossierO,OcteauV,SibaritaJB,LeducC,TessierB,NairD,GatterdamV,DestaingO,AlbigesRizoC,TampéR,CognetL,ChoquetD,LounisB,GiannoneG.(2012)Integrinsfunctionas
homotypicstableadhesionunitstransientlytrappedinadhesionsites.
NatureCellBiol14,1057-1067.http://dx.doi.org/10.1038/ncb2588
40)
HartmannJ,TranTV,KaudeerJ,OberleK,HerrmannJ,QuaglianoI,AbelT,CohnenA,
GatterdamV,JacobsA,WollscheidB,TampéR,WatzlC,DiefenbachA,KochJ(2012)Thestalk
domainandtheglycosylationstatusoftheactivatingnaturalkillercellreceptorNKp30are
importantforligandbinding.JBiolChem287,31527-39.
http://dx.doi.org/10.1074/jbc.M111.304238
41)
HulpkeS,TomiokaM,KremmerE,UedaK,AbeleR,TampéR(2012)Directevidencethatthe
N-terminalextensionsoftheTAPcomplexactasautonomousinteractionscaffoldsforthe
assemblyoftheMHCIpeptide-loadingcomplex.CellMolLifeSci69,3317-27.
http://dx.doi.org/10.1007/s00018-012-1005-6
42)
DemirelÖ,BangertI,WoltersD,BlanzJ,SaftigP,TampéR,AbeleR(2012)Thelysosomal
polypeptidetransporterTAPLisstabilizedbytheinteractionwithLAMP-1andLAMP-2.
JCellSci125,4230-40.http://dx.doi.org/10.1242/jcs.087346
43)
HinzA,TampéR(2012)ABCtransportersandimmunity.Biochemistry51,4981-9.
http://dx.doi.org/10.1021/bi300128f
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44)
WuS,Avila-SakarA,KimJM,BoothDS,GreenbergCH,RossiA,LiaoM,AlianA,GrinerSL,Juge
N,MergelCM,Chaparro-RiggersJ,StropP,TampéR,EdwardsRH,StroudRM,CraikCS,Cheng
Y(2012)FabsenablesingleparticlecryoEMstudiesofsmallproteins.Structure20,582-592.
http://dx.doi.org/10.1016/j.str.2012.02.017
45)
WeiR,GatterdamV,WienekeR,TampéR*,RantU*(2012)Stochasticsensingofproteinswith
receptor-modifiedsolid-statenanopores.NatureNanotechnol7,257-63.(*shared
correspondingauthorship)http://dx.doi.org/10.1038/nnano.2012.24
Views&Newsreview:L.Q.Gu,B.Ritzo,Y.Wang(2012)Biosensing:Whenlessismoreina
nanopore.NatureNanotechnol.7,212-213.http://dx.doi.org/10.1038/nnano.2012.48
46)
GatterdamV,StoessT,MengeCM,HeckelA,TampéR(2012)Cagedglutathione–triggering
proteininteractionbylight.AngewChemIntEd51,3726-9.
http://dx.doi.org/10.1002/anie.201108073
47)
WyciskAI,LinJ,LochS,HobohmK,FunkeJ,KochJ,SkachWR,MayerhoferPU,TampéR(2011)
Epstein-BarrviralBNLF2ainsertsastail-anchoredproteinpost-translationallyintotheER
membraneandarrestsTAPinatransport-incompetentconformation.JBiolChem286,4140212.http://dx.doi.org/10.1074/jbc.M111.237784
48)
BarthelmeD,DinkelakerS,AlbersSV,LondeiP,ErmlerU,TampéR(2011)Ribosomerecycling
dependsonamechanisticlinkbetweentheFeSclusterdomainandaconformationalswitchof
thetwin-ATPaseABCE1.ProcNatlAcadSciUSA108,3228-3233.
http://dx.doi.org/10.1073/pnas.1015953108
49)
AbeleR,TampéR(2011)TheTAPtranslocationmachineryinadaptiveimmunityandviral
escapemechanisms.EssaysBiochem50,249-64.http://dx.doi.org/10.1042/bse0500249
50)
RoizardS,DanelonC,HassaineG,PiquetJ,SchulzeK,HoviusR,TampéR,Vogel,H(2011)
ActivationofGproteincoupledreceptorsinnativeplasmamembranesupportedonbeads.J
AmChemSoc133,16868-74.http://dx.doi.org/10.1021/ja205302g
51)
GrunwaldC,SchulzeK,GiannoneG,CognetL,LounisB,ChoquetD,TampéR(2011)Quantum
yieldoptimizedfluorophoresforsite-specificlabelingandsuper-resolutionimaging.JAm
ChemSoc133,8090-8093.http://dx.doi.org/10.1021/ja200967z
HighlightedasAdvanceattheFrontiersofPhotochemicalSciencesintheyear2011
52)
SchölzC,ParcejD,EjsingCS,RobenekH,UrbatschIL,TampéR(2011)Specificlipidsmodulate
thetransporterassociatedwithantigenprocessing(TAP).JBiolChem286,13346-56.
http://dx.doi.org/10.1074/jbc.M110.216416
53)
ZutzA,HoffmannJ,HellmichUA,GlaubitzC,LudwigB,BrutschyB,TampéR(2011)Asymmetric
ATPhydrolysiscycleoftheheterodimericmultidrugABCcomplexTmrABfromThermus
thermophilus.JBiolChem286,7104-15.http://http://dx.doi.org/10.1074/jbc.M110.201178
54)
HergetM,BaldaufC,SchölzS,ParcejD,WiesmüllerKH,TampéR,AbeleR,BordignonE(2011)
ConformationofantigenicpeptidesboundtotheABCtransporterTAP.ProcNatlAcadSciUSA
108,1349-54.http://dx.doi.org/10.1073/pnas.1012355108
55)
SchnellRamosMS,AbeleR,GrotemeyerMS,NagyR,TampéR,TentschD,MartinoiaE(2011)
Characterizationofatransportactivityforlong-chainpeptidesinbarleymesophyllvacuoles.J
ExpBot62,2403-10.http://dx.doi.org/10.1093/jxb/erq397
56)
TampéR,SchleiffE(2011)Highlight:Membranetransportinlightofstructure,function,and
evolution.BiolChem392,3-3.http://dx.doi.org/10.1515/BC.2011.019
57)
GhanemE,FritzscheS,Al-BalushiM,HashemJ,GhuneimL,ThomerL,KalbacherH,vanEndert
P,WiertzE,TampéR,SpringerS(2011)Thetransporterassociatedwithantigenprocessing
(TAP)isactiveinapost-ERcompartment.JCellScience123,4271-9.
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KleefenA,PedoneD,GrunwaldC,WeiR,FirnkesM,AbstreiterG,RantU,TampéR(2010)
Multiplexedparallelsingletransportrecordingsonnanoporearrays.NanoLett10,5080-7.
http://dx.doi.org/10.1021/nl1033528
59)
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160) SchmittL,TampéR(2000)Affinity,specificity,diversity–AchallengefortheABC-transporter
TAPincellularimmunity.ChemBioChem1,16-35.
161) RädlerU,MackJ,PersikeN,JungG,TampéR(2000)Anovelapproachtocreatetetheredlipid
membranesviatheNTA-histidinesystem.BiophysJ79,3144-52.
162) DetmersJM,AbeleR,LanfermeijerFC,JackR,KoningsWN,TampéR,PoolmanB(2000)
Combinatorialpeptidelibrariesrevealtheligandbindingpropertiesoftheoligopeptide
receptorOppAofLactococcuslactis.ProcNatlAcadSciUSA97,12487-92.
163) UebelS,TampéR(1999)SpecificityoftheproteasomeandtheTAPtransporter.
CurrOpinImmunol11,203-8.
164) DornIT,EscherichR,SeemüllerE,GuckenbergerR,TampéR(1999)High-resolutionAFMimagingandmechanisticanalysisofthe20Sproteasome.JMolBiol288,1027-37.
165) AbeleR,TampéR(1999)FunctionoftheTAPtransportcomplexinthecellularimmune
recognition.BiophysBiochemActa1461,405-19.
166) PfänderR,NeumannL,ZweckstetterM,SegerC,HolakTA,TampéR(1999)Structureofthe
activedomainoftheherpessimplexvirusproteinICP47inwater/sodiumdodecylsulfate
solutiondeterminedbynuclearmagneticresonancespectroscopy.Biochemistry38,13692-8.
167) Lankat-ButtgereitB,TampéR(1999)ThetransporterassociatedwithantigenprocessingTAP:
Structureandfunction.FEBSLett464,108-12.
168) NeumannL,TampéR(1999)KineticofpeptidebindingtothetransportcomplexTAP–Evidence
forstructuralrearrangementsinducedbysubstratebinding.JMolBiol294,1203-13.
169) SeligerB,HardersC,LohmannS,MomburgF,UrlingerS,TampéR,HuberC(1998)DownregulationoftheMHCclassIantigen-processingmachineryafteroncogenictransformationof
murinefibroblasts.EurJImmunol28,122-33.
170) DornIT,HofmannUG,PeltonenJ,TampéR(1998)Diacetylenechelatorlipidassupportfor
immobilizationandimagingofproteinsbyscanningforcemicroscopy.Langmuir14,6620-4.
171) DornIT,NeumaierKR,TampéR(1998)Molecularrecognitionofhistidine-taggedmoleculesby
chelatorlipidsmonitoredbyfluorescenceenergytransferandcorrelationspectroscopy.
JAmChemSoc120,2753-63.
172) RädlerU,HeizC,LuisiPLTampéR(1998)Basepairformationofself-organizingRNAamphiphileswithintwo-dimensions.Langmuir14,6620-4.
173) DornIT,PawlitschkoK,PettingerSC,TampéR(1998)Orientationandtwo-dimensional
organizationofproteinsatchelatorlipidinterfaces.BiolChem379,1151-9.
174) DietrichC,MerkelR,TampéR(1997)Diffusionmeasurementoffluorescencelabeled
amphiphilicmoleculeswithastandardfluorescencemicroscope.BiophysJ72,170110.
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175) BeinertD,NeumannL,UebelS,TampéR(1997)StructuralchangesofICP47,theviralinhibitor
ofTAP,inducedbymembraneassociation.Biochemistry36,4694-700.
176) SeligerB,HöhneA,JungD,KallfezM,KnuthA,JaegerE,BernhardH,MomburgF,TampéR,
HuberC(1997)Expressionandfunctionofthepeptidetransporterinescapevariantsofhuman
renalcellcarcinomas.ExpHematology25,608-14.
177) ZeidlerR,EißnerG,MeißnerP,UebelS,TampéR,LazisS,HammerschmidtW(1997)
DownregulationofTAP1inBlymphocytesbycellularandEpstein-Barrvirus-encoded
interleukin-10.Blood90,2390-7.
178) OrtmannB,CopemanJ,SadasivanB,HerbergJ,TampéR,TrowsdaleJ,CresswellP(1997)
MultivalentassociationofMHCclassI-tapasincomplexeswiththetransporterassociatedwith
antigenprocessing.Science277,1306-9.
179) UrlingerS,KuchlerK,MeyerTH,UebelS,TampéR(1997)Intracellularlocation,complex
formation,andfunctionofthetransporterassociatedwithantigenprocessing(TAP)inyeast.
EurJBiochem245,266-72.
180) Vives-PiM,VargasF,CostaM,SospedraM,SomozaN,ObiolsG,TampéR,TrowsdaleJ,James
RFL,Pujol-BorrellR(1997)Proteasomesubunits,lowmolecularmasspolypeptides-2and-7
(LMP2&LMP7)arehyperexpressedbytargetcellsinautoimmunethyroiddisease(AITD)but
notininsulindependentdiabetesmellitus(IDDM):acluetopathogenesis?
TissueAntigens50,153-63.
181) NeumannL,KrassW,UebelS,JungG,TampéR(1997)ActivedomainoftheHerpesvirus
proteinICP47-apotentinhibitorofthetransporterassociatedwithantigenprocessing.
JMolBiol272,484-92.
182) WagnerD,HofmannUG,DornI,SchmittL,TampéR,GaubHE(1997)AnomalouspH
dependenceofthecoexistencepressureofthepolymerizabletwo-chainN-lipidmethylbis(pentacosadiinoyl-oxyethyl)-amine.EurBiophysJ26,271-5.
183) UebelS,PlantingaT,WeberPJA,Beck-SickingerAG,TampéR(1997)Peptidebindingand
photo-crosslinkingtodetergentsolubilizedandtoreconstitutedtransporterassociatedwith
antigenprocessing(TAP).FEBSLett416,359-63.
184) UebelS,KraasW,KienleS,WiesmüllerKH,JungG,TampéR(1997)Recognitionprincipleof
TAPasdisclosedbycombinatorialpeptidelibraries.ProcNatlAcadSciUSA94,8976-81.
185) WhiteCA,ThomsonSA,CooperL,vanEndertPM,TampéR,CouparB,QiuL,ParsonsPG,Moss
DJ,KhannaR(1997)ConstitutivetransductionofpeptidetransporterandHLAgenesrestores
antigen-processingfunctionandcytotoxicT-cell-mediatedimmunerecognitionofhuman
melanomacells.IntJCancer75,590-5.
186) SchmittL,BohanonTM,DenzingerS,RingsdorfH,TampéR(1996)Specificproteindockingto
chelatorlipidmonolayersmonitoredbyFT-IRattheair-waterinterface.
AngewChemieIntEdEngl35,317-20.
187) DietrichC,BoscheinenO,ScharfKD,SchmittL,TampéR(1996)Functionalimmobilizationofa
DNA-bindingproteinatmembraneinterfacesviaspecificbindingtoasyntheticchelatorlipid.
Biochemistry35,1100-5.
188) ChenHL,GabrilovichD,VirmaniA,TampéR,GirgisKR,GazdarAF,CarboneDP(1996)A
functionallydefectivealleleofTAP1resultsinlossofMHCclassIantigenpresentationina
humanlungcancer.NatureGenetics13,210-3.
189) EhringB,MeyerTH,EckerskornC,LottspeichF,TampéR(1996)EffectsofMHC-encoded
subunitsonthepeptidaseandproteolyticactivitiesofhuman20Sproteasomes:Cleavageof
proteinsandantigenicpeptides.EurJBiochem235,404-15.
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190) SeligerB,HöhneA,KnuthA,BernhardH,MeyerT,TampéR,MomburgF,HuberC(1996)
AnalysisofthemajorhistocompatibilitycomplexclassIantigenpresentationmachineryin
normalandmalignantrenalcells:evidencefordeficienciesassociatedwithtransformationand
progression.CancerResearch56,1756-60.
191) SchmittL,TampéR(1996)SynthesisandcharacterizationofATP-lipids:proteinanchorand
energysourceintwodimensions.JAmChemSoc118,5532-45.
192) AhnK,MeyerTH,UebelS,SempéP,DjaballahH,PetersonPA,FrühK,TampéR(1996)
MolecularmechanismandspeciesspecificityofTAPinhibitionbyHerpessimplexvirusprotein
ICP47.EMBOJ15,3247-55.
193) SeligerB,HöhneA,KnuthA,BernhardH,EhringB,TampéR,HuberC(1996)Reduced
membranemajorhistocompatibilitycomplexclassIdensityandstabilityinasubsetofhuman
renalcellcarcinomaswithlowTAPandLMPexpression.ClinicalCancerResearch2,1427-33.
194) GritschS,NeumaierK,SchmittL,TampéR(1995)Engineeredfusionmoleculesatchelatorlipid
interfacesimagedbyreflectioninterferencecontrastmicroscopy(RICM)
Biosensors&Bioelectronics10,805-12.
195) DietrichC,TampéR(1995)Chargedeterminationofmembranemoleculesinpolymersupportedlipidlayers.BiochimBiophysActa1238,183-91.
196) DietrichC,SchmittL,TampéR(1995)Molecularorganizationofhistidine-taggedbiomolecules
atself-assembledlipidinterfacesusinganovelclassofchelatorlipids.
ProcNatlAcadSciUSA92,9014-8.
197) UebelS,MeyerTH,KraasW,KienleS,JungG,WiesmüllerKH,TampéR(1995)Requirements
forpeptidebindingtothehumantransporterassociatedwithantigenprocessingrevealedby
peptidescansandcomplexpeptidelibraries.JBiolChem270,18512-6.
198) FrühK,AhnK,DjaballahH,SempéP,vanEndertPM,TampéR,PetersonPA,YangY(1995)A
viralinhibitorofpeptidetransportersforantigenpresentation.Nature375,415-8.
199) SchmittL,DietrichC,TampéR(1994)Synthesisandcharacterizationofchelator-lipidsfor
reversibleimmobilizationofengineeredproteinsatself-assembledlipidinterfaces.
JAmChemSoc116,8485-91.
200) KühnerM,TampéR,SackmannE(1994)Lipidmono-andbilayersupportedonpolymerfilms–
compositepolymer-lipidfilmsonsolidsubstrates.BiophysJ67,217-26.
201) MüllerKM,EbenspergerE,TampéR(1994)Nucleotidebindingtothehydrophilic,C-terminal
partofthehumantransporterassociatedwithantigenprocessing(TAP).
JBiolChem269,14032-7.
202) MeyerTH,vanEndertPM,UebelS,EhringB,TampéR(1994)Functionalexpressionand
purificationofthetransporterassociatedwithantigenprocessingininsectcells.
FEBSLett351,443-7.
203) vanEndertPM,TampéR,MeyerTH,TischR,BachJF,McDevittHO(1994)Asequentialmodel
ofpeptidebindingandtransportbythetransportersassociatedwithantigenprocessing.
Immunity1,491-500.
204) CloverLM,SargentIL,TownsendA,TampéR,RedmanCWG(1994)ExpressionofTAP1by
humantrophoblast.EurJImmunol25,543-8.
205) BrinkG,SchmittL,TampéR,SackmannE(1994)Self-assemblyofcovalentlyanchored
phospholipidsupportedmembranesbyuseofDODA-Suc-NHS-lipids.
BiochimBiophysActa1196,227-30.
206) TampéR,vonLukasA,GallaHJ(1991)Glycophorininducedcholesterol-phospholipid-domains
indimyristoylphosphatidylcholinebilayervesicles.Biochemistry30,4909-16.
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207) TampéR,GallaHJ(1991)SynergisticeffectsofCa2+andwheatgermagglutininonthelamellarhexagonal(HII)phasetransitionofglycophorincontainingeggphosphatidylethanolamine
membranes.EurJBiochem199,187-93.
208) TampéR,McConnellHM(1991)KineticsofantigenicpeptidebindingtotheclassIIMHC
moleculeIAd.ProcNatlAcadSciUSA88,4661-45.
209) TampéR,ClarkBR,McConnellHM(1991)Energytransferbetweentwopeptidesboundtoone
MHCclassIImolecule.Science254,87-9.
210) TampéR,TyvollD,McConnellHM(1991)ReactionsofthesubunitsoftheclassIImajor
histocompatibilitycomplexmoleculeIAd.ProcNatlAcadSciUSA88,10667-70.
211) TampéR,RobitzkiA,GallaHJ(1989)Interactionbetweenglycophorinandaspinlabeled
cholesterolanalogueinreconstituteddimyristoylphosphatidylcholinebilayervesicles.
BiochimBiophysActa982,41-6.
212) TampéR,WinterA,WohlfartP,BeckerJ,GallaHJ(1989)ReconstitutionandEPR-spectroscopic
characterizationofglycophorincontainingphospholipidvesicles.ChemPhysLipids51,91-103.
213) OllmannM,TampéR,WinterA,WohlfartP,GallaHJ(1988)Interactionofgangliosideand
glycophorincarbohydrateswithmembranesurfaces.BerBunsengesPhysChem92,982-5.
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Patents
1) TampéR(2000)SchaltbarebiochemischePinzettenundsynthetischeRezeptorenzur
molekularenOrganisationundManipulationvonBiomolekülen.DE10064896.7;
PCT/EP01/15210
2) BaldaufC,SchulzeK,BordingonE,TampéR(2011)Hochaffinemultivalente
Chelatorverbindungen(MCHs)undderenVerwendungzurStruktur-undFunktionsanalysevon
Zielmolekülen;PCT/EP2011/052451
Books,BookArticles,Editorials
1) TampéR(2015)MembraneTransportandCommunicationinFrankfurt:Speakers’Summary.
BiolChem396,949-54.http://dx.doi.org/10.1515/hsz-2015-0211
2) TampéR.(2009)Membranetransportandcommunication.Highlight:thegatekeepersoflife
yieldtheirsecrets.Biol.Chem.390,673.
3) Loch,S.&Tampé,R.(2005)Immunevasine–Tricks,wieVirendemImmunsystem
entkommen.BioSpektrum2,157-161.
4) Beismann-Driemeyer,S.&Tampé,R.(2002)ThepeptidetransporterTAPincellularimmunity
andviruspersistence.InMembraneTransporterDiseases.BroerandWagner,Eds,Kluwer
Academic/PlenumPublishers,NewYork,pp319-338.
5) Lankat-ButtgereitB,TampéR(2002)Thetransporterassociatedwithantigenprocessing
(TAP):Apeptidetransportandloadingcomplexessentialforcellularimmuneresponse.ABC
Proteins:InFromBacteriatoMan(eds.I.B.Holland,K.Kuchler,C.Higgins,S.Cole).Academic
PressLtd,London.
6) UebelS,WiesmüllerKH,JungG,TampéR(1999)Peptidelibrariesincellularimmune
recognition.InCurr.TopicsinMicrobiologyandImmunology,eds.Winnacker,Wong&
Famulok,Springer,Heidelberg,pp1-27.
7) TampéR(1998)Das"ABC"derImmunologie–FunktiondesPeptid-Transport-komplexesTAP
beiderzellulärenImmunerkennung.BIOspektrum4,23-29.
8) TampéR,UrlingerS,PawlitschkoK,UebelS(1997)Thetransporterassociatedwithantigen
processing(TAP).InUnusualSecretoryPathways:FromBacteriatoMan.eds.Kuchler,
Rubartelli&Holland,Springer,NewYork,pp115-136.
9) UebelS,TampéR(1997)ProcessingandselectionofantigensbytheMHC-encodedpeptide
transporterTAP.InSymposiuminImmunologyVI-Vaccination,eds.Eibl,Huber,Peter&
Wahn,Springer,NewYork,pp155-163.
10) TampéR,DietrichC,GritschS,ElenderG,SchmittL(1996)Biofunctionalizedmembraneson
solidsurfaces.InNanofabricationandBiosystems:FrontiersandChallenges,eds.Hoch,
Jelinski,Craighead,CambridgeUniversityPress,pp201-221.
11) JungG,NiedermannG,EichmannK,UebelS,TampéR,WaldenP,IhlenfeldtHG,KraasW,
KienleS,WiesmüllerKH(1996)Processing,transport,andMHCrestrictedpresentationof
antigensstudiedbypeptidelibraries.InPeptides:Chemistry,Structure,andBiology,eds.
Kaumaya&Hodges,MayflowerScientificLtd,pp761-763.
AbstractsandProceedings(>200)arenotlisted
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RobertTampé
Keynote&HonoraryLectures,ConferenceOrganization
• Organizer,FEBSInternationalConference“ABCproteinsinHealthandDiseases”2014
• SessionChair,FEBSInternationalConferenceonABCProteins2012
• Chair,FASEBSymposium“TransportATPase”,Colorado2012
• Organizer,InternationalMolecularLifeScienceConference2011
• SessionChair,GordonConferenceonMembraneProteins2008,2010
• KeynoteLecture,FEBSInternationalConferenceonABCProteins2008
• KeynoteLecture,TransportSymposium,Vienna2008
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2014(M) World Megamarathon Ranking 300+

VL Zelluläre Biochemie Klausur 9. August 2016 Master Biochemie