MAP Kinase Signaling
MAPK/Erk in Growth and Differentiation Pathway
Integrins
RTKs
Ion Channels
[Ca2+]
[Ca2+]
Src
PYK2
Spry
PI3K
B-Raf
Erk1
C-TAK1
cPLA2
Translation
Control
Late Endosome
Erk1/2
lasm
Ets
Elk-1
TIF1A
Stat1/3
c-Myc/
N-Myc
Pax6
c-Fos
IκBα
GSK-3
DAPK
METTL1
BAD
nNos
Bim
WAVE-2
Ca2+-regulated
Exocytosis
PLD1
Migration
PPARγ
FoxO3
p27 KIP1
MSK1/2
Erk1/2
ER
eIF4B
TSC2
Cytoplasmic Anchoring
MKP-1/2
TCPβ
eEF2K
p90RSK
MKP-3
cdc25
us
Nucle
FMK
SL0101
BI-D1870
Erk1/2
MNK1/2
p90RSK
Cytop
SOS
MEK1/2
PEA-15
Cell Adhesion
PP1/
PP2At
KSR
1/2
rpS6
Filamin A
PD98059
U0126
AZD6244
IMP
Ion Channels,
Receptors
LKB1
14-3-3
Tpl2/Cot1
MP1
mTOR
Signaling
AMPK
Heterodimer
LAMTOR2
EPAC
Raptor
c-Raf B-Raf
MEK1
C3G
PKA
c-Raf
PKC
Rap1
Rac [cAMP]
PAK
Spred
Tal
CAS RACK1
Fyn
Pax FAK
CRK
SOS
GRB2
PLCγ
Ras
SOS
GRB2
Shc
FRS2
IRS
RTKs
CREB
ER81
SRF
c-Fos
ERα
Nur77
MITF
C/EBPβ
Histone
H3
HMGN1
ETV1
TIF1A
ATF4
Myt1
Mad1
YB1
Transcription
www.cellsignal.com/cstmapk
BUB1
ATF1
UBF
For pathway key and background please visit:
p90RSK
Key Products for MAP Kinase Signaling
Visit www.cellsignal.com/mapklist for full listing of antibodies, related kits, and reagents.
ANTIBODIES
#8662 ASK1 (D11C9) Rabbit mAb
APPLICATIONS
REACTIVITY
WB, IP
H, M, R, Mk
#3765 Phospho-ASK1 (Thr845) Antibody
WB
H, (M)
#9226 ATF-2 (20F1) Rabbit mAb
WB, IP, IHC-P
H, M, R, Mk
#9221 Phospho-ATF-2 (Thr71) Antibody
WB, IP, IHC-P, IHC-F, IF-IC, F
H, M, R, Mk
#2250 c-Fos (9F6) Rabbit mAb
WB, IF-IC, F, ChIP
H, M, R, (Hm, B, Pg)
#5348 Phospho-c-Fos (Ser32) (D82C12) XP® Rabbit mAb
WB, IP, IF-IC, F, ChIP
H, M, R, (Hm, B, Pg, Hr)
#9165 c-Jun (60A8) Rabbit mAb
WB, IP, IHC-P, IHC-F, IF-IC, ChIP H, M, R, Mk
#2361 Phospho-c-Jun (Ser63) (54B3) Rabbit mAb
WB, IHC-P
#3270 Phospho-c-Jun (Ser73) (D47G9) XP® Rabbit mAb
WB, IP, IHC-P, IF-IC, F, ChIP
H, M, R, Mk, Pg, (B)
#3058 DUSP6/MKP3 Antibody
WB
H
H, M, R
#3372 Erk5 Antibody
WB, IP
H, M, R, Mk
#3371 Phospho-Erk5 (Thr218/Tyr220) Antibody
WB
H, M, R, Mk
#9258 JNK2 (56G8) Rabbit mAb
WB
H, M, R, Hm, Mk, Mi
#3042 MAPKAPK-2 Antibody
WB, IP, F
H, M, R, Mk, (Hm)
#3007 Phospho-MAPKAPK-2 (Thr334) (27B7) Rabbit mAb
WB, IHC-P, IF-IC, F
H, M, R, Mk
#2352 MEK1 (61B12) Mouse mAb
WB, IP
H, M, R, Mk
#9126 MEK1/2 (47E6) Rabbit mAb
WB
H, M, R, Mk, (Dg)
#9154 Phospho-MEK1/2 (Ser217/221) (41G9) Rabbit mAb
WB, IP
H, M, R, Mk, Dm, (C)
H, M, (R, B, Hr)
#4994 NIK Antibody
WB
#8690 p38 MAPK (D13E1) XP® Rabbit mAb
WB, IHC-P, IF-IC, F
H, M, R, Hm, Mk, Pg, (C)
#4511 Phospho-p38 MAPK (Thr180/Tyr182) (D3F9) XP® Rabbit mAb
WB, IP, IHC-P, IF-IC, F
H, M, R, Mk, Mi, Pg, Sc,
(Hm, C, Z, B, Pg)
#9216 Phospho-p38 MAPK (Thr180/Tyr182) (28B10) Mouse mAb
WB, IP, IF-IC, F
H, M, R, Mk, Sc, (Z)
#4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb
WB, IP, IHC-P, IF-IC, F
H, M, R, Hm, Mk, Mi,
Dm, Z, B, Dg, Pg, Ce, (C)
#4370 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (D13.14.4E)
XP® Rabbit mAb
WB, IP, IHC-P, IF-IC, F
H, M, R, Hm, Mk, Mi, Dm,
Z, B, Dg, Pg, Sc, (C, Ce)
#9106 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10)
Mouse mAb
WB, F
H, M, R, Hm, Mk, Mi, Dm,
Z, B, Pg
#2708 p70 S6 Kinase (49D7) Rabbit mAb
WB, IHC-P
H, M, R, Mk
#9234 Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb
WB
H, M, R, Mk, (C)
#9433 B-Raf (55C6) Rabbit mAb
WB
H, M, R, Mk
#2696 Phospho-B-Raf (Ser445) Antibody
WB
H, M, R, Mk, (C, Dg)
#9422 c-Raf Antibody
WB
H, M, R, Mk
#9421 Phospho-c-Raf (Ser259) Antibody
WB, IP
H, M, R, Mk, X, (C)
#9427 Phospho-c-Raf (Ser338) (56A6) Rabbit mAb
WB
H, M, R, Mk
#9252 SAPK/JNK Antibody
WB
H, M, R, Hm, Mk, Z, B, Sc
#4668 Phospho-SAPK/JNK (Thr183/Tyr185) (81E11) Rabbit mAb
WB, IP, IHC-P
H, M, R, Dm, Sc
#9255 Phospho-SAPK/JNK (Thr183/Tyr185) (G9) Mouse mAb
WB, IP, IF-IC, F
H, M, R, Hm, Sc
#9152 SEK1/MKK4 Antibody
WB, IP
H, M, R, Mk, (Dg)
#9156 Phospho-SEK1/MKK4 (Ser257/Thr261) Antibody
WB
H, M, R, Dm, (X, Z)
#5206 TAK1 (D94D7) Rabbit mAb
WB, IP
H, M, R
#9339 Phospho-TAK1 (Ser412) Antibody
WB, IP
H, M, R
#8955 Ras (D2C1) Rabbit mAb
#14429 Ras (G12D Mutant Specific) (D8H7) Rabbit mAb
#14412 Ras (G12V Mutant Specific) (D2H12) Rabbit mAb
#9355 RSK1/RSK2/RSK3 (32D7) Rabbit mAb
#11989 Phospho-p90RSK (Ser380) (D3H11) Rabbit mAb
WB
H, M, R, Mk
WB
H, (M, R)
WB
H, (M, R)
WB, IP, IF-IC
H, M, R, Mk
WB, IHC-P, IF-IC
H, M, R, Mk, Mi,
(C, X, Z, B, Dg, Pg, Hr)
Phospho-p44/42
MAPK (Erk1/2)
(Thr202/Tyr204)
(D13.14.4E) XP®
Rabbit mAb #4370:
Confocal IF analysis of
C2C12 cells, treated
with U0126 #9903
(10 μM, 1 hr; top) or
TPA #9905 (200 nM,
15 min; bottom), using
#4370 (green). Actin
filaments were labeled
with Alexa Fluor® 555
Phalloidin #8953 (red).
Blue pseudocolor =
DRAQ5® #4084 (fluorescent DNA dye).
Phospho-p38 MAPK
A
(Thr180/Tyr182) (D3F9)
®
XP Rabbit mAb #4511:
WB analysis (A) of extracts
from COS and 239 cells,
untreated (-) or UV-treated
(+) using #4511 (upper)
or p38 MAPK Antibody
#9212 (lower). Flow
cytometric analysis (B)
of Jurkat cells, untreated
(blue) or treated with Anisomycin #2222 (green),
using #4511 compared
to a nonspecific negative
control antibody (red).
kDa
100
80
293
COS
60
50
Phosphop38 MAPK
40
30
20
10
100
80
60
50
p38 MAPK
40
30
20
10
–
+
–
+
UV
B
Events
ELISA KITS
Phospho-c-Jun
(Ser73) (D47G9) XP®
Rabbit mAb #3270:
IHC analysis of paraffinembedded human lung
carcinoma using #3270.
#7274 PathScan® MAP Kinase Multi-Target Sandwich ELISA Kit
#7029 PathScan® Phospho-MEK1 (Ser217/221) Chemiluminescent Sandwich ELISA Kit
15FLYMAPK_REFM105ENG_00
#7946 PathScan® Phospho-p38 MAPK (Thr180/Tyr182) Sandwich ELISA Kit
#7177 PathScan® Phospho-p44/42 MAPK (Thr202/Tyr204) Sandwich ELISA Kit
#7849 PathScan® Phospho-SAPK/JNK (Thr183/Tyr185) Chemiluminescent Sandwich ELISA Kit
Phospho-p38 MAPK (Thr180/Tyr182)
APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry
/ -P Paraffin / -F Frozen / E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebra fish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all
species expected / ( ) 100% sequence homology
© 2015 Cell Signaling Technology, Inc. Cell Signaling Technology, PathScan, and XP are trademarks of Cell Signaling Technology, Inc. U.S. Patent No. 5,675,063. All other trademarks are the property of their respective owners.
For Research Use Only. Not For Use In Diagnostic Procedures.
in Deutschland und Österreich exklusiv von:
New England Biolabs GmbH, Brüningstr. 50, Geb. B852, 65926 Frankfurt/Main, Germany
Tel: +49/(0)69/305-23140
www.cellsignal.de
e-mail: [email protected]
Cell Signaling Technology Europe, Schuttersveld 2, 2316 ZA Leiden, The Netherlands
Tel. +31 (0)71 568 1060
www.cellsignal.com
e-mail: [email protected]