Jonas Aretz Curriculum vitae Pestalozzistraße 22 10625 Berlin H +49 (176) 44 55 73 98 B [email protected] Key skills: Fragment-based drug design Protein expression and purification Education 2012–2016 Desired degree: PhD, Freie Universitaet Berlin and Max Planck Institute of Colloids and Interfaces, Potsdam, finish 07/2016. Biochemistry 2009–2012 Master of Science, Bielefeld University, 1.0. Molecular Biotechnology 2006–2009 Bachelor of Science, Bielefeld University, 1.1, year’s best student. Molecular Biotechnology Publications 2016 Chemical fragment arrays for rapid druggability assessment J Aretz, Y Kondoh, K Honda, UR Anumala, M Nazare, N Watanabe, H Osada, C Rademacher, Chem Commun, Fragment-screening of N-acetylmannosamine kinase reveals non-carbohydrate inhibitors J Aretz, PR Wratil, E-C Wamhoff, HG Nguyen, W Reutter, C Rademacher, Can J Chem, 2015 Fragmentbasierte Wirkstoffentwicklung J Aretz, C Rademacher, Nachr Chem, 63(2):116 2014 Computational and experimental prediction of human C-type lectin receptor druggability J Aretz, EW Wamhoff, J Hanske, D Heymann, C Rademacher, Front Immunol, 5:323 Carbohydrate-lectin recognition of sequence-defined heteromultivalent glycooligomers D Ponader, P Maffre, J Aretz, D Pussak, NN Ninnemann, S Schmidt, PH Seeberger, C Rademacher, GU Nienhaus, L Hartmann, JACS, 136(5):2008 Epitope recognition of antibodies against a Yersinia pestis lipopolysaccharide trisaccharide component F Broecker, J Aretz, Y Yang, J Hanske, X Guo, A Reinhardt, A Wahlbrink, C Rademacher, C Anish, PH Seeberger, ACS Chem Biol, 9(4):867 In Structure activity relationship of thiazolopyrimidine derivatives binding to the preparation murine C-type lectin receptor Langerin J Aretz, UR Anumala, J Schulze, M Nazare, C Rademacher Not peer-reviewed publications and talks 2015 Fragment-based design of novel lectin ligands Talk at Ringberg Conference of Max Planck Institute of Colloids and Interfaces, Kreuth 2014 Computational and Experimental Prediction of Human C-Type Lectin Receptor Druggability Poster at Fragment-based lead discovery, Basel Fragment-based screening against DC-SIGN using fluorine NMR Poster at 36th Discussion Meeting of the GDCh-Division of "Magnetic Resonance", Berlin 2013 Understanding cell behavior in cultivation processes - A metabolic approach J Aretz, T Thuete, S Scholz, K Kersting, T Noll, H Buentemeyer, BMC Proc, 7(Suppl 6):P90, Poster with abstract for 23rd ESACT meeting. Fragment-based design of novel lectin ligands Talk at Science X talks symposium, Amsterdam 2012 The Bisphenol A-Team: A Cell-Free Approach for Biosensors Poster at Strategic Process Biotechnology 2020+, Berlin 2011 The Bisphenol A-Team: A Cell-Free Approach for Biosensors Poster at iGEM european jamboree, Amsterdam Poster at iGEM international jamboree, Cambridge, MA In cooperation with media office of Bielefeld University: Press releases about the project and the competition Talk at Science Café Bielefeld Establishing a Luminescent Biosensor in E. coli : The Modulated Acetosyringone Receptor Sensing System F Haenisch*, J Aretz*, E Brombacher*, N Kessler*, NC Luebke*, J Marschall*, A Neshat*, S Unthan*, F Walter*, T Wolf*, C Rueckert, J Kalinowski and K Niehaus, Article on Promega Pub Hub MARSS: Modulated Acetosyringone Receptor Sensor System Talk and poster at 6th CeBiTec symposium, Bielefeld Poster at Strategic Process Biotechnology 2020+, Berlin 2010 MARSS: Modulated Acetosyringone Receptor Sensor System Talk and poster at iGEM international jamboree, Boston, MA In cooperation with media office of Bielefeld University: Press releases about the project and the competition Talk at Science Café Bielefeld Languages German Mother tongue English Fluent French Basic Stay abroad 2015 Six weeks research exchange at Chemical Biology Research Group, RIKEN Center for Sustainable Resource Science, Wako, Japan Computer skills Very good LATEX, MS office, OriginPro, MestReNova, KNIME Good Graphpad Prism, InstantJChem Basic Berkeley Madonna, Cytoscape, JMP10, MOE Experience and relevant skills: PhD 2012–2016 Non-carbohydrate Scaffolds for Targeted Delivery into Cells expressing the C-type Lectin Langerin, Max Planck Institute of Colloids and Interfaces, Potsdam. Aretz et al., in preparation - Set-up and quality control of a fragment library - Fragment screening using STD NMR and T2 filtered 1 H NMR - Design, analysis and interpretation of SARs using SPR and IC50 assays 2013–2014 Computational and Experimental Prediction of Human C-Type Lectin Receptor Druggability, Max Planck Institute of Colloids and Interfaces, Potsdam. Aretz et al. (2014) Front Immunol 5:323. - Expression and purification of different C-type lectin receptors - Druggability assessment using 19 F and T2 filtered 19 F NMR 2015 Chemical micro arrays for fragment and high-throughput screening, RIKEN institute, Wako. Aretz et al. (2016) Chem Commun - Establishment of a fragment-based druggability assessment using chemical micro arrays - High-throughput screening of a natural products library using chemical micro arrays 2013–2016 Crystallisation of mLangerin and DC-SIGN, Freie Universitaet, Berlin. In cooperation with and performed in Prof. Dr. Markus Wahl’s lab - Crystallisation of C-type lectin receptors using different crystallization systems (96 well plate with robotic pipetting platform, 48 well plate sitting drop, 24 well plate hanging drop) - Structure of mLangerin could be solved, manuscript in preparation 2012–2016 Fragment-based design of novel DC-SIGN ligands, Max Planck Institute of Colloids and Interfaces, Potsdam. Aretz et al., in preparation - Fragment screening using SPR and plate based IC50 assays - Hit validation using SPR, IC50 assays and 15 N HSQC NMR 2012–2014 Epitope mapping of carbohydrate-protein interactions, Max Planck Institute of Colloids and Interfaces, Potsdam. Ponader et al. (2014) JACS, 136(5):2008; Broecker et al. (2014) ACS Chem Biol, 9(4):867. - Epitope mapping using STD NMR Experience and relevant skills: MSc and BSc 2011–2012 Characterizing the metabolism and growth of the human Age1.hn AAT cell line using continuous cultivations, Bielefeld University. Aretz et al. (2013) BMC proc 7(Suppl 6):P90 - Metabolomics of glycolysis, TCA, nucleotides and activated sugars (with LC-ESITripleQuad-MS) - cell culture technology, continuous cultivations, mathematic modelling of cultivations 2011 iGEM 2011: The Bisphenol A-Team: A Cell-free Approach for Biosensors, Bielefeld University. - Production (7 L) and purification of recombinant S-layer fusion proteins Establishment of a BPA analysis (by LC-ESI-qTOF-MS/MS) Characterization and optimization of BPA degradation by recombinant E. coli Results: Gold medal, Qualification for world championship, Sweet sixteen 2010 iGEM 2010: MARSS - Modulated Acetosyringone Receptor Sensor System, Bielefeld University. - Synthetic biology using BioBricks (synthesizing, cloning, sequencing, characterization) - Directed evolution, site-directed mutagenesis - Results: Gold medal 09/2008 Internship, Fraunhofer-Institut fuer Molekularbiologie und Angewandte Oekologie, Aachen. Bioanalytics - SDS-PAGE, protein crystallography References Dr. Christoph Rademacher Group leader of Structural Glycobiology Lab, Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, D-14476 Potsdam, Phone: +49 (331) 567 9358, [email protected] Prof. Dr. Markus Wahl Head of Structural Biochemistry Lab, Freie Universitaet Berlin, Takustrasse 6, D-14195 Berlin, Phone: +49 (30) 838 53410, [email protected] Prof. Dr. Thomas Noll Deputy Scientific Director of Bielefeld Center for Biotechnology (CeBiTec), Bielefeld University, Post Box 10 01 31, D-33501 Bielefeld, Phone: +49 (521) 106 6319, [email protected] Berlin, April 6, 2016 Jonas Aretz
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