Jonas Aretz – Curriculum vitae

Jonas Aretz
Curriculum vitae
Pestalozzistraße 22
10625 Berlin
H +49 (176) 44 55 73 98
B [email protected]
Key skills:
Fragment-based drug design
Protein expression and purification
Education
2012–2016 Desired degree: PhD, Freie Universitaet Berlin and Max Planck Institute of Colloids and
Interfaces, Potsdam, finish 07/2016.
Biochemistry
2009–2012 Master of Science, Bielefeld University, 1.0.
Molecular Biotechnology
2006–2009 Bachelor of Science, Bielefeld University, 1.1, year’s best student.
Molecular Biotechnology
Publications
2016 Chemical fragment arrays for rapid druggability assessment
J Aretz, Y Kondoh, K Honda, UR Anumala, M Nazare, N Watanabe, H Osada, C
Rademacher, Chem Commun,
Fragment-screening of N-acetylmannosamine kinase reveals non-carbohydrate inhibitors
J Aretz, PR Wratil, E-C Wamhoff, HG Nguyen, W Reutter, C Rademacher, Can J Chem,
2015 Fragmentbasierte Wirkstoffentwicklung
J Aretz, C Rademacher, Nachr Chem, 63(2):116
2014 Computational and experimental prediction of human C-type lectin receptor druggability
J Aretz, EW Wamhoff, J Hanske, D Heymann, C Rademacher, Front Immunol, 5:323
Carbohydrate-lectin recognition of sequence-defined heteromultivalent glycooligomers
D Ponader, P Maffre, J Aretz, D Pussak, NN Ninnemann, S Schmidt, PH Seeberger, C
Rademacher, GU Nienhaus, L Hartmann, JACS, 136(5):2008
Epitope recognition of antibodies against a Yersinia pestis lipopolysaccharide
trisaccharide component
F Broecker, J Aretz, Y Yang, J Hanske, X Guo, A Reinhardt, A Wahlbrink, C Rademacher,
C Anish, PH Seeberger, ACS Chem Biol, 9(4):867
In Structure activity relationship of thiazolopyrimidine derivatives binding to the
preparation murine C-type lectin receptor Langerin
J Aretz, UR Anumala, J Schulze, M Nazare, C Rademacher
Not peer-reviewed publications and talks
2015 Fragment-based design of novel lectin ligands
Talk at Ringberg Conference of Max Planck Institute of Colloids and Interfaces, Kreuth
2014 Computational and Experimental Prediction of Human C-Type Lectin Receptor
Druggability
Poster at Fragment-based lead discovery, Basel
Fragment-based screening against DC-SIGN using fluorine NMR
Poster at 36th Discussion Meeting of the GDCh-Division of "Magnetic Resonance", Berlin
2013 Understanding cell behavior in cultivation processes - A metabolic approach
J Aretz, T Thuete, S Scholz, K Kersting, T Noll, H Buentemeyer, BMC Proc, 7(Suppl
6):P90, Poster with abstract for 23rd ESACT meeting.
Fragment-based design of novel lectin ligands
Talk at Science X talks symposium, Amsterdam
2012 The Bisphenol A-Team: A Cell-Free Approach for Biosensors
Poster at Strategic Process Biotechnology 2020+, Berlin
2011 The Bisphenol A-Team: A Cell-Free Approach for Biosensors
Poster at iGEM european jamboree, Amsterdam
Poster at iGEM international jamboree, Cambridge, MA
In cooperation with media office of Bielefeld University: Press releases about the project
and the competition
Talk at Science Café Bielefeld
Establishing a Luminescent Biosensor in E. coli : The Modulated Acetosyringone
Receptor Sensing System
F Haenisch*, J Aretz*, E Brombacher*, N Kessler*, NC Luebke*, J Marschall*, A Neshat*,
S Unthan*, F Walter*, T Wolf*, C Rueckert, J Kalinowski and K Niehaus, Article on
Promega Pub Hub
MARSS: Modulated Acetosyringone Receptor Sensor System
Talk and poster at 6th CeBiTec symposium, Bielefeld
Poster at Strategic Process Biotechnology 2020+, Berlin
2010 MARSS: Modulated Acetosyringone Receptor Sensor System
Talk and poster at iGEM international jamboree, Boston, MA
In cooperation with media office of Bielefeld University: Press releases about the project
and the competition
Talk at Science Café Bielefeld
Languages
German Mother tongue
English Fluent
French Basic
Stay abroad
2015 Six weeks research exchange at Chemical Biology Research Group, RIKEN Center for
Sustainable Resource Science, Wako, Japan
Computer skills
Very good LATEX, MS office, OriginPro, MestReNova, KNIME
Good Graphpad Prism, InstantJChem
Basic Berkeley Madonna, Cytoscape, JMP10, MOE
Experience and relevant skills: PhD
2012–2016 Non-carbohydrate Scaffolds for Targeted Delivery into Cells expressing the C-type
Lectin Langerin, Max Planck Institute of Colloids and Interfaces, Potsdam.
Aretz et al., in preparation
- Set-up and quality control of a fragment library
- Fragment screening using STD NMR and T2 filtered 1 H NMR
- Design, analysis and interpretation of SARs using SPR and IC50 assays
2013–2014 Computational and Experimental Prediction of Human C-Type Lectin Receptor
Druggability, Max Planck Institute of Colloids and Interfaces, Potsdam.
Aretz et al. (2014) Front Immunol 5:323.
- Expression and purification of different C-type lectin receptors
- Druggability assessment using 19 F and T2 filtered 19 F NMR
2015 Chemical micro arrays for fragment and high-throughput screening, RIKEN institute,
Wako.
Aretz et al. (2016) Chem Commun
- Establishment of a fragment-based druggability assessment using chemical micro arrays
- High-throughput screening of a natural products library using chemical micro arrays
2013–2016 Crystallisation of mLangerin and DC-SIGN, Freie Universitaet, Berlin.
In cooperation with and performed in Prof. Dr. Markus Wahl’s lab
- Crystallisation of C-type lectin receptors using different crystallization systems (96 well
plate with robotic pipetting platform, 48 well plate sitting drop, 24 well plate hanging
drop)
- Structure of mLangerin could be solved, manuscript in preparation
2012–2016 Fragment-based design of novel DC-SIGN ligands, Max Planck Institute of Colloids
and Interfaces, Potsdam.
Aretz et al., in preparation
- Fragment screening using SPR and plate based IC50 assays
- Hit validation using SPR, IC50 assays and 15 N HSQC NMR
2012–2014 Epitope mapping of carbohydrate-protein interactions, Max Planck Institute of Colloids and Interfaces, Potsdam.
Ponader et al. (2014) JACS, 136(5):2008; Broecker et al. (2014) ACS Chem Biol, 9(4):867.
- Epitope mapping using STD NMR
Experience and relevant skills: MSc and BSc
2011–2012 Characterizing the metabolism and growth of the human Age1.hn AAT cell line
using continuous cultivations, Bielefeld University.
Aretz et al. (2013) BMC proc 7(Suppl 6):P90
- Metabolomics of glycolysis, TCA, nucleotides and activated sugars (with LC-ESITripleQuad-MS)
- cell culture technology, continuous cultivations, mathematic modelling of cultivations
2011 iGEM 2011: The Bisphenol A-Team: A Cell-free Approach for Biosensors, Bielefeld
University.
-
Production (7 L) and purification of recombinant S-layer fusion proteins
Establishment of a BPA analysis (by LC-ESI-qTOF-MS/MS)
Characterization and optimization of BPA degradation by recombinant E. coli
Results: Gold medal, Qualification for world championship, Sweet sixteen
2010 iGEM 2010: MARSS - Modulated Acetosyringone Receptor Sensor System, Bielefeld University.
- Synthetic biology using BioBricks (synthesizing, cloning, sequencing, characterization)
- Directed evolution, site-directed mutagenesis
- Results: Gold medal
09/2008 Internship, Fraunhofer-Institut fuer Molekularbiologie und Angewandte Oekologie,
Aachen.
Bioanalytics
- SDS-PAGE, protein crystallography
References
Dr. Christoph Rademacher
Group leader of Structural Glycobiology Lab, Max Planck Institute of Colloids and Interfaces,
Am Mühlenberg 1, D-14476 Potsdam, Phone: +49 (331) 567 9358,
[email protected]
Prof. Dr. Markus Wahl
Head of Structural Biochemistry Lab, Freie Universitaet Berlin, Takustrasse 6, D-14195
Berlin, Phone: +49 (30) 838 53410,
[email protected]
Prof. Dr. Thomas Noll
Deputy Scientific Director of Bielefeld Center for Biotechnology (CeBiTec), Bielefeld
University, Post Box 10 01 31, D-33501 Bielefeld, Phone: +49 (521) 106 6319,
[email protected]
Berlin, April 6, 2016
Jonas Aretz